Wet Lab Procedures $200.00: Registration Form

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WET LAB PROCEDURES $200.00

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Wet Lab Assignment TLC © 6/1/2018 2 www.abctlc.com

Wet Lab Answer Key
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Wet Lab Procedures CEU Training Course Assignment
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mail it back to TLC.
You will have 90 days from the start of this course to complete in order to receive your
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and the completed manual to [email protected].
We would prefer that you utilize the enclosed answer sheet in the front, but if you are
unable to do so, type out your own answer key. Please include your name and address
on your manual and make copy for yourself.
1. Most pathogens are generally associated with diseases that _________and affect
people in a relatively short amount of time, generally a few days to two weeks.
A. Limits the treatment process D. Will cause fatalities
B. Are mild in nature E. Limit the travel of pathogens
C. Cause intestinal illness F. None of the Above
How Diseases Are Transmitted.

2. Waterborne pathogens are primarily spread by the?
A. Fecal-oral, or feces-to-mouth, route D. Influenza route
B. Dermal to fecal route E. Waterborne mishaps
C. Oral to fecal route F. None of the Above
3. When infecte humans or animals pass the bacteria, viruses, and _______________in
their stool, pathogens may get into water and spread disease.
A. Fecal Coliform and E coli D. Cryptosporidiosis
B. Protozoa E. Bioslime
C. Macroorganisms F. None of the Above
4. For another person to become infected, he or she must take that pathogen in through
the mouth.
A. True B. False
5. This term means when in nature it is different from other types of pathogens such as
the viruses that cause influenza (the flu) or the bacteria that cause tuberculosis.
A. Fecal Coliform and E coli D. Waterborne Pathogen(s)
B. Giardia lamblia E. Coliform bacteria
C. Microorganism(s) F. None of the Above
6. According to the text, these are spread by secretions that are coughed or sneezed
into the air by an infected person.
A. Fecal Coliform and E coli D. Influenza virus and tuberculosis bacteria
C. Microorganisms F. None of the Above

Chain of Transmission
7. The pathogens must survive in the water, that will depend on the temperature of the
water and the length of time the __________________are in the water.
A. Stomach bugs D. Germs
B. Turbidity E. Pathogens
C. Microscopic particles F. None of the Above
8. Which pathogen may survive for months such as Giardia or?

A. Illness D. Campylobacteriosis
B. Cryptosporidium E. Tampylobacteriosis
C. Bacteria F. None of the Above
9. This chain lists the events that must occur for the transmission of disease via drinking
water. By breaking the chain at any point, the Transmission of disease will be prevented.
A. True B. False
10. Water must have feces and must contain this term to cause a waterborne disease.
A. Campylobacteriosis D. Fecal-oral material
B. Pathogens E. Contaminated water
C. Waterborne illness(es) F. None of the Above
Viral-Caused Diseases
11. Which of the following terms is an example of a common viral disease that may be
transmitted through water?
A. Pathogen D. Campylobacteriosis
B. Yersiniosis E. Incubation period
C. Hepatitis A F. None of the Above
12. The onset is usually abrupt with fever, malaise, loss of appetite, nausea and
abdominal discomfort, followed within a few days by jaundice.
A. True B. False
13. Which of the following terms in drinking water can be inactivated by chlorine or other
disinfectants?
A. Illnesses D. Pathogen(s)
B. Giardiasis E. Infections
C. Viruses F. None of the Above
The Main Players- History and Biology Chapter 1

Circumstances under which Koch’s postulates do not easily apply
14. According to the text, which of the following terms may develop only when an
opportunistic pathogen invades a susceptible host?
A. Disease(s) D. Divide
B. Mutation(s) E. Reproduction
C. Carriers F. None of the Above
15. Which of the following terms are caused by dietary deficiencies?

A. Disease(s) D. Pathogen(s)
B. Mutation(s) E. Microorganisms

16. According to the text, which of the following terms are very difficult to grow under in-
vitro (in the laboratory) conditions?
A. Disease(s) D. Pathogen(s)
B. Mutation(s) E. Microbes
17. Fastidious organisms can now be grown in cultures of human or animal cells or in
small animals.
A. True B. False
18. Not all laboratory animals are susceptible to all?

A. Pathogens D. Disease
B. Secondary invaders E. Chemical reactions
19. Some of the diseases are inherited or are caused by abnormality in chromosomes
are influenced by?
A. Environmental factors D. Disease
B. Secondary invaders E. Chemical reactions
Bacteria
20. Bacteria are prokaryotes (Kingdom Monera), which means that they have No true
nucleus. They do have one chromosome of double-stranded DNA in a ring.
A. True B. False
21. There are some bacteria relatives that can do photosynthesis--they don’t have
chloroplasts, but their ___________and other needed chemicals are built into their cell
membranes.
A. Chlorophyll D. Double-stranded DNA
B. An organelle E. Bacilli
C. Cellulose F. None of the Above
22. Bacteria consist of only?

A. Chloroplasts D. Double-stranded DNA
B. An organelle E. A single cell
23. Pathogens have been found that can live in temperatures above the boiling point
and in cold that would freeze your blood. They "eat" everything from sugar and starch to
sunlight, sulfur and iron.
A. True B. False
Prokaryotes
24. The only prokaryotes are Bacteria and archaea all other life forms are
_________creatures whose cells have nuclei.
A. Bacteria D. Eukaryotes
B. Peptidoglycan E. Microorganism
C. Bacilli F. None of the Above

Early Origins
25. Bacteria, are basically one of three different shapes, some are rod - or stick-shaped
and called Bacilli. Others are shaped like little balls and called cocci (cox-eye).
A. True B. False
26. According to the text, bacterial cells exist as individuals while others cluster together
to form?
A. AN organism D. Pairs, chains, squares or other groupings
B. An organelle E. Helical or spiral in shape
27. The mitochondria that make energy for your body cells is one example of?
A. Chloroplasts D. Bacilli
B. An organelle E. Eukaryote(s)
28. A single teaspoon of topsoil contains more than a billion (1,000,000,000) bacteria.
A. True B. False
Peptidoglycan
29. The amount and location of the _________are different in the two possible types of
cell walls, depending on the species of bacterium.
A. Capsule D. Cell membrane/plasma membrane/cytoplasmic membrane
B. Peptidoglycan E. True nucleus
C. Cytoplasmic granules F. None of the Above
30. Penicillin, inhibit the formation of the chemical cross linkages needed to make?
A. Disease(s) D. Peptidoglycan
B. Mutation(s) E. Bacteria
31. If a person stops an antibiotic, any living bacteria could start making
_____________, grow, and reproduce.
A. Bacteria D. Germ theory of disease
C. Eukaryotes F. None of the Above
Metabolism
32. Which of the following is a term that describes all the chemical reactions by which
food is transformed for use by the cells?
A. Fastidious D. Germ theory of disease
B. Metabolism E. Osmosis
C. Chemical reactions F. None of the Above
33. A cell can grow through its metabolism, reproduce and it can respond to changes in
its environment.
A. True B. False

Gram Stain
34. One of the two possible types of ___________has more peptidoglycan than the
other.
A. Bacteria D. Bacterial cell walls
B. Peptidoglycan E. Gram stain
C. Gram+ or Gram - F. None of the Above
35. Which type of bacteria often incorporate toxic chemicals into their cell walls, and
thus tend to cause worse reactions in our bodies?
A. Positive D. Gram+
B. Fastidious E. Gram -
C. Gram or Gram F. None of the Above
+ -
36. Which of the bacteria have less peptidoglycan, antibiotics like penicillin are less
effective against them?
+ -
37. Pseudomonas aeruginosa is a strictly aerobic, oxidase positive, non-fermentative

bacterium are?
B. Fastidious E. Gram negative
38. In the Gram process, the amount of peptidoglycan in the cell walls of the bacteria
under study will determine how those bacteria absorb the dyes with which they are
stained; thus, bacterial cells can be Gram+ or Gram -.
A. True B. False
39. Which type of bacteria have simpler cell walls with lots of peptidoglycan, and stain a
dark purple color?
A. Aerobic D. Gram+
B. Positive E. Gram -
C. Gram or Gram
+ - F. None of the Above
40. Which type of bacteria have more complex cell walls with less peptidoglycan, thus
absorb less of the purple dye used and stain a pinkish color?
+ -
41. The Gram-stain appearance is not particularly characteristic although rods are
somewhat thinner than those seen for the?
A. Coliform bacteria D. HPC
B. Enteric-like bacteria E. CFU
C. Standard plate count F. None of the Above

Two types of cells- Procaryotes and Eucaryotes
42. Which of the following terms exhibits all the characteristics of life but it lacks the
complex system of membranes and organelles?
A. Coliform bacteria D. HPC
B. Enteric-like bacteria E. CFU
C. Standard plate count F. None of the Above
43. Which cell has a complex structure, containing a true nucleus and many membrane
bound organelles?
A. Eukaryote(s) D. Protozoan
B. Vesicles E. Paramecium
C. Prokaryote(s) F. None of the Above
Structure of a Eukaryotic Cell

44. Cell Membrane: The cell is enclosed and held intact by the cell membrane/plasma
membrane/cytoplasmic membrane and is composed of large molecules of proteins and?
A. Capsule D. Phospholipids
B. Cell wall E. True nucleus
45. Which of the following terms is selectively permeable?

A. Cytoplasmic granules D. A single circular DNA molecule
B. Cellular membrane E. DNA and proteins
C. Cell wall F. None of the Above
Nucleus
46. Which of the following terms is enclosed in the nuclear membrane and contains
chromosomes?
A. Chromosomes D. Macromolecular polymer-peptidoglycan
B. Nucleus E. Cytoplasmic organelles
C. Cell membrane F. None of the Above
47. A single circular DNA molecule consists of many genes. A gene is a coiled unit made
up of Cytoplasmic granules and proteins that code for or determine a particular
characteristic of an individual organism.
A. True B. False
Cytoplasm
48. Cytoplasm is composed of a semifluid gelatinous nutrient matrix and cytoplasmic
organelles including endoplasmic reticulum, ribosomes, Golgi complex, mitochondria,
________________, microtubules, lysosomes and vacoules.
A. Chromosomes D. Centrioles
B. Procaryotes E. Cytoplasmic organelles
Cell Wall
49. Which of the following is found as an external structure of plant cells, algae, and
fungi?
B. Cilia E. DNA and proteins
C. A cell wall F. None of the Above

Cilia and Flagella
50. Which of the following terms express cells that possess relatively long and thin
structures called Flagella?
A. Eukaryotic D. Protozoan
B. Vesicles E. Paramecium
C. Prokaryotic F. None of the Above
51. Which of the following terms are also organs of locomotion but are shorter and more
numerous?
A. Cytoplasmic granules D. Flagella
B. Cilia E. Hair
Structure of a Procaryotic Cell

52. All bacteria are procaryotes and are simple cells and they divide by binary fission.
A. True B. False
Chromosome
53. The chromosome of a prokaryotic cell usually consists of a single circular
_____________ and serves as the control center of the bacterial cell.
B. DNA molecule E. DNA and proteins
54. A typical bacterial chromosome contains approximately 10,000 genes.

A. True B. False
Cytoplasm
55. Which of the following terms is a semi-liquid that surrounds the chromosome and is
contained within the plasma membrane?
B. Cytoplasm E. Cytoplasmic organelles
56. Which of the following terms occur in certain species of bacteria which can be
specifically stained and used to identify the bacteria?
A. Cytoplasm D. Cell membrane/plasma membrane/cytoplasmic
membrane
Cell Membrane
57. The Cell Membrane is similar to that of the?
C. Eukaryotic cell membrane F. None of the Above

58. Which of the following is selectively permeable and controls the substances entering
or leaving the cell?
C. Eukaryotic cell membrane F. None of the Above
Capsules
59. Some bacteria have a layer of material outside the?
A. Capsule D. Cell membrane/plasma membrane/cytoplasmic membrane
60. Which of the following terms, is highly organized and firmly attached, this layer is
called a capsule or if it is not highly organized and not firmly attached, a slime layer?
A. Capsule D. DNA and proteins
61. Which of the following terms consist of complex sugars or polysaccharides

combined with lipids and proteins?
A. Cytoplasmic granules D. Capsules
B. Cilia E. DNA and proteins
62. Which of the following terms is useful in differentiating between different types of
bacteria?
A. Capsule D. Cell membrane/plasmamembrane/cytoplasmic
63. Which of the following terms are usually detected by negative staining, where the
bacterial cell and the background become stained but the capsule remains unstained?
B. Capsules E. DNA and proteins
C. Encapsulated bacteria F. None of the Above
64. Encapsulated bacteria produce colonies on Flagellated bacteria are smooth, mucoid
and glistening, whereas the capsulated bacteria produce rough and dry colonies.
A. True B. False
65. Capsules enable the bacterial species to attach to mucus membranes and protect
the bacteria from phagocytosis.
A. True B. False
Flagella
66. Peritrichous bacteria- possess?
A. One flagellum at each end D. Genetic material from one bacteria
B. Tuft of flagella E. Flagella over the entire surface
C. The entire surface F. None of the Above

67. Flagella are ____________that enable the bacteria to move.
A. Cytoplasmic granules D. False feet
B. Cilia E. Hair
C. Thread-like proteins F. None of the Above
68. The following term is said to be motile while non-flagellated bacteria are
generally non-motile.
A. Bacteria D. Flagellated bacteria
69. Lophotrichous bacteria-possess at one or both ends?

A. Forming spores D. Tuft of flagella
B. Spore formation E. Cilia
C. A single polar flagellum F. None of the Above
70. Amphitrichous bacteria-bacteria with?

A. One flagellum at each end D. Transfer genetic material from one bacteria cell to
another
B. A single polar flagellum E. One or both ends
71. Monotrichous bacteria-bacteria with?

A. One flagellum at each end D. Genetic material from one bacteria cell to another
B. A single polar flagellum E. One or both ends
Pili or Fimbriae
72. Pili or Fimbriae enable the bacteria to attach to other bacteria or to membrane
surfaces such as intestinal linings or?
B. RBC E. Cytoplasmic organelles
73. Which of the following terms is used to transfer genetic material from one bacteria
cell to another?
B. Pili or Fimbriae E. Cytoplasmic organelles
Spores
74. Which of the following terms is enclosed in several protein coats that are resistant to
heat, drying and most chemicals?
A. Spores D. Spore formation
B. Genetic material E. Macromolecular polymer-peptidoglycan

75. Which of the following terms lands on a nutrient rich surface, can form a new
vegetative cell?
A. Spores D. Spore formation
B. Genetic material E. Dried spore
C. Several protein coats F. None of the Above
76. Spore formation is related to the survival of bacterial cells, not reproduction.
A. True B. False
Bacterial Nutrition
77. Which of the following terms may in significant quantities, but some seem to only
need it in trace amounts?
A. Water D. Oxygen
B. Nitrogen E. Calcium
C. Iron, Zinc, Cobalt F. None of the Above
78. Which of the following terms all life requires in order to grow and reproduce?
A. Water D. Oxygen
B. Copper E. Calcium
79. Which of the following terms are required by some enzymes to function?
A. Water D. Oxygen
B. Copper E. Calcium
80. All life has the same basic nutritional requirements which include: Energy. This may
be light or inorganic substances like sulfur, carbon monoxide or ammonia, or preformed
organic matter like sugar, protein, fats etc.
A. True B. False
81. Which of the following terms may be nitrogen gas, ammonia, nitrate/nitrite, or a
nitrogenous organic compound like protein or Nucleic acid?
A. Water D. Oxygen
B. Nitrogen E. Calcium
82. Which of the following terms may be carbon dioxide, methane, carbon monoxide, or
a complex organic material?
A. Water D. Oxygen
B. Carbon E. Calcium
83. Which of the following terms may require gaseous air?

A. Water D. Oxygen
B. DNA molecule E. Calcium

Fastidious
84. Which of the following terms may synthesize every complex molecule they need
from the basic minerals?
A. Eukaryote(s) D. Centrioles
B. Bacteria E. Viruses
85. Which of the following terms are said to be fastidious, require preformed organic
molecules like vitamins, amino acids, nucleic acids, carbohydrates?
What in the World is an Eukaryote?

86. Which of the following terms represents animals, plants, and fungi, which are mostly
multicellular, as well as various other groups called protists, many of which are
unicellular?
87. Which of the following terms represents organisms such as bacteria lack nuclei and
other complex cell structures?
88. The eukaryotes share a common origin, and are often treated formally as a super
kingdom, empire, or domain.
A. True B. False
Eukaryotic Cells
89. According to the text, Eukaryotic cells are generally much larger
than____________, typically with a thousand times their volumes.
90. Many cells ingest food and other materials through a process of osmosis, where the
outer membrane invaginates and then pinches off to form a Flagella.
A. True B. False
91. Which of the following terms is surrounded by a double membrane, with pores that
allow material to move in and out?
A. The nucleus D. Cilia
B. Flagella E. Cell wall
C. DNA molecule F. None of the Above

92. Which of the following terms represents a variety of Internal membranes and
structures, called organelles, and a cytoskeleton composed of microtubules and
microfilaments?
93. Which of the following terms represent this DNA which is divided into several
bundles called chromosomes, which are separated by a microtubular spindle during
nuclear division?
94. Which of the following terms represent cells that include a variety of membrane-
bound structures, collectively referred to as the endomembrane system.
B. Golgi bodies or dictyosomes E. Eukaryotic
Protozoan Reservoirs of Disease
95. Which of the following bugs/disease/causes terms represents the causative
organism of Legionnaires' disease?
A. Centrioles D. Amoebae
B. Viruses E. Bacterium Legionella pneumophila
C. Autotropic F. None of the Above
96. Which of the following bugs/disease/causes terms live and reproduce in the
cytoplasm of some free-living amoebae?
A. Centrioles D. Amoebae
B. Viruses E. Bacterium Legionella pneumophila
C. Autotropic F. None of the Above
97. The presence of bacteria in the cytoplasm of protozoa is well known, whereas that
of viruses is less frequently reported. Most of these reports simply record the presence
of bacteria or viruses and assume some sort of symbiotic relationship between them and
the Protozoa.
A. True B. False
98. Which of the following terms were shown to not only survive but also to multiply in
the cytoplasm of free-living, nonpathogenic protozoa?
A. Human pathogens D. Soil-dwelling protozoa
B. Foraminifera E. Marine protozoa
C. Freshwater protozoan F. None of the Above
99. Protozoa are the natural habitat for certain pathogenic bacteria.
A. True B. False

Symbionts
100. Which of the following terms inhabit the rumen and reticulum of ruminates and the
cecum and colon of equids?
A. Protozoa D. Soil-dwelling protozoa
B. Foraminifera E. Ciliates
Viruses
101. Which term are acellular microorganisms, made up of only genetic material and a
protein coat?
A. Viruses D. Antiviral therapies
B. Genetic material E. Virions
C. Poxviruses F. None of the Above
102. Which term depend on the energy and metabolic machinery of the host cell to
reproduce?
A. Fungi D. Virus(es)
103. Viruses are found in virtually all life forms, including humans, animals, plants,
___________, and bacteria.
A. Fungi D. Antiviral therapies
104. According to the text, viruses consist of genetic material—either
__________________or ribonucleic acid (RNA)—surrounded by a protective coating of
protein, called a capsid, with or without an outer lipid envelope.
A. Deoxyribonucleic acid (DNA) D. Antiviral therapies
105. Which of the following terms are between 20 and 100 times smaller than bacteria
and hence are too small to be seen by light microscopy?
A. Viruses D. Fungi
106. Which of the following terms vary in size from the largest poxviruses of about 450
nanometers in length to the smallest polioviruses of about 30 nanometers?
A. Viruses D. Bacteria
107. Which of the following terms are not considered free-living, since they cannot
reproduce outside of a living cell; they have evolved to transmit their genetic information
from one cell to another for the purpose of replication?
A. Viruses D. Bacteria

108. Which of the following terms often damage or kill the cells that they infect, causing
disease in infected organisms?
109. Because of the difficulty in developing antiviral therapies stems from the large
number of variant ________________ that can cause the same disease, as well as the
inability of drugs to disable a virus without disabling healthy cells.
110. With the development of antiviral agents is a major focus of current research, and
the study of _______________has led to many discoveries important to human health.
111. A few viruses stimulate cells to grow uncontrollably and produce cancers. Although
many infectious diseases, such as the common cold, are caused by viruses, there are
no cures for these illnesses.
A. True B. False
Virions
112. Which of the following terms is a complete functional virus that has the capacity to
infect living tissue?
A. Podoviruses D. Phage lambda of E. coli
B. Phage's host range E. A virion
C. Myovirus bacteriophages F. None of the Above
113. If the cell was burst artificially, then these virus particles cannot be called virion
because they will lack certain proteins that will make them infectious even though the
___________is present.
A. Podoviruses D. Genetic material
B. Phage's host range E. Viral genome
114. According to the text, biomolecules found in virions: genetic material,

_____________, single or double stranded, nucleoprotein capsid.
A. Either DNA or RNA D. Phage lambda of E. coli
B. Phage's host range E. Viral genome
Bacteriophage
115. According to the text, bacteriophages consist of ___________enclosing genetic
material.
A. Bacteriophages D. Peptidoglycan
B. Phages E. An outer protein hull
C. Microbial mats F. None of the Above

116. One of the densest natural sources for phages and other viruses is sea water,
where up to 9×108 virions per milliliter have been found in _________ at the surface,
and up to 70% of marine bacteria may be infected by phages.
B. Phages E. Virions
117. The genetic material can be ssRNA (single stranded RNA), dsRNA, ssDNA, or
dsDNA between 5 and 500 kilo base pairs long with linear arrangement. Bacteriophages
are much smaller than the Plasma membrane they destroy - usually between 20 and
200 nm in size.
A. True B. False
118. Which creature or substance is estimated to be the most widely distributed and
diverse entities in the biosphere?
B. Phages E. Virions
119. Which creature or substance is ubiquitous and can be found in all reservoirs
populated by bacterial hosts, such as soil or the intestine of animals?
A. Host cell secretion D. Plasma membrane
B. Phage(s) E. Bacterial hosts
C. Lysogenic F. None of the Above
120. Phages may be released via cell lysis or by?

121. Which phages does not kill the host but rather become long-term parasites and
make the host cell continually secrete more new virus particles?
Protozoa
122. When protozoa are in the form of _______________, they actively feed and grow.
A. Cysts D. Hermaphroditic
B. Trophozoites E. Apicomplexans
C. Pathogens F. None of the Above
123. Which bug/creature/organism/species play a role both as herbivores and as

consumers in the decomposer link of the food chain?
A. Protozoa D. Algal production
B. Malaria parasites E. Trophozoites and cysts
C. Microinvertebrates F. None of the Above

124. Which bug/creature/organism/species are an important food source for
microinvertebrates?
A. Meiofauna D. Algal production
B. Malaria parasites E. Protozoa
125. The ecological role of protozoa in the transfer of bacterial and _______________
to successive trophic levels is important.
126. According to the text, the process by which the protozoa takes its cyst form is
called encystation, while the process of transforming back into _______________ is
called excystation.
B. Trophozoite E. Apicomplexans
127. Protozoa occupy a range of trophic levels, as predators, they prey upon unicellular
or filamentous algae, bacteria, and?
A. Microfungi D. Algal production
128. Most protozoa exist in 5 stages of life which are in the form of ________________.
129. Which bug/creature/organism/species can survive harsh conditions, such as

exposure to extreme temperatures and harmful chemicals, or long periods without
access to nutrients, water, or oxygen for a period of time.
130. An individual protozoan is?

B. Trophozoite E. Apicomplexans
131. Which bug/creature/organism/species are around 10–50 micrometer, but can grow
up to 1 mm and can easily be seen under a microscope.

132. Which bug/creature/organism/species exist throughout aqueous environments and
soil.
Ecological Role of Protozoa

133. Which of the following terms represents an organism which is frequently
overlooked, these play an important role in many communities where they occupy a
range of trophic levels?
A. Protozoa D. Fossil foraminifera
134. According to the text, these are predators of unicellular or filamentous algae,
________, and microfungi, protozoa play a role both as herbivores and as consumers in
the decomposer link of the food chain.
A. Bacteria D. Pathogenic bacteria
B. Many ecological conditions E. Bacterium
C. Amazingly diverse organisms F. None of the Above
135. The ecological role of Foraminifera in the transfer of bacterial and algal production
to successive trophic levels is important.
A. True B. False
Factors Affecting Growth and Distribution

136. Which of the following terms reproduce by cell division?
A. Most free-living protozoa D. Fossil foraminifera
Wastewater Bug Section

137. In the Activated Sludge process, the ____________are also called waste activated
sludge.
A. Organisms D. Secondary treatment
B. Settled bugs E. Sludge Volume Index
C. Mixed liquor F. None of the Above
138. The waste sludge is treated separately. The remaining wastewater is now much
cleaner. In fact, after primary and ____________, about 85% or more of all pollutants in
the wastewater has been removed and it goes on to Disinfection.
A. Oxygen D. Secondary treatment
B. Settled bugs E. Settleable Solids
C. Activated sludge F. None of the Above
139. The first group is the bacteria which eat the dissolved organic compounds is
generally four (4) groups of bugs that do most of the “eating” in this process.
A. Mixed liquor D. Secondary treatment
B. Settled bugs E. Total Dissolved Solids

140. The second and third groups of bugs are microorganisms known as the free-
swimming and?
A. Mixed liquor D. Bacteria
B. Suctoria E. Volatile Solids
C. Stalked ciliates E. None of the Above
141. This bug which feeds on the larger bugs and assist with settling is in the fourth
group, known as?
A. Water bear D. Rotifer
B. Suctoria E. Vorticella
C. Activated sludge bugs F. None of the Above
142. The Bacteria have several interesting properties--their “fat reserve” is stored on the
outside of their body and this strange feature?
A. Fur D. No Mouth
B. Feet E. No Cilia
C. Eyes F. None of the Above
143. Once the bacteria have “contacted” their food, they start the digestion process, a
chemical Enzyme is sent out through the cell wall to break up the?
A. Mixed liquor D. Bacteria
B. Organic compounds E. Total Dissolved Solids
144. The cell is highly engineered and because of this hydrolytic enzyme, it breaks the
organic molecules into small units which are able to pass through the cell wall of the?
A. Mixed bugs D. Bacteria
B. Compound E. Protozoan
C. Organism F. None of the Above
145. In wastewater treatment, the process of using bacteria-eating-bugs in the presence

of oxygen to reduce the organics in water is called?
A. Mixed liquor D. Reduction
B. Oxidation E. Settleable Solids
146. Activated Sludge: The first step in the process, the contact of the bacteria with the
organic compounds, takes about?
A. 24 hours D. 30 Minutes
B. 2 Hours E. 72 Hours
C. 1 Hour F. None of the Above
147. An asset in settling the bug is its fat storage property and as the bugs “bump” into
each other, the fat on each of them sticks together and causes flocculation of the?
A. Mixed liquor D. WAS
B. Floc E. Settleable Solids
C. Non-organic solids and biomass F. None of the Above

148. What does facultative mean as far as bugs? What environments are they
adaptable to survive and multiply in?
A. Either anaerobic or aerobic conditions D. Aerobic only
B. Anaerobic only E. Volatile
C. Facultative F. None of the Above
149. The next step as in the text, this substance, which is the activated sludge, is used
again by returning it to the influent of the aeration tank for mixing with the primary
effluent and ample amounts of air?
A. Carry over D. Super WAS
B. RAS E. Sludge Volume Index
C. Solids biomass F. None of the Above
150. We need to be able to properly identify the bugs and this common bugs are a
medium size to large swimming Ciliate, commonly observed in activated sludge,
sometimes in abundant numbers.
A. Vorticella D. Euchlanis
B. Euglypha E. Rotifer
C. Paramecium F. None of the Above
Giardiasis Giardia lamblia

151. According to the text, Giardia lamblia (intestinalis) is a single celled animal, i.e., a
protozoon, that moves with the aid of five flagella. In Europe, it is sometimes referred
to as?
A. Chronic cases D. Typically, the disease
B. The organism E. Morphologically distinct organism
C. Lamblia intestinalis F. None of the Above
152. Giardiasis is the most frequent cause of non-bacterial diarrhea in North America.
Giardia duodenalis, cause of giardiasis, is a one-celled, Microscopic parasite that can
live in the intestines of animals and people. It is found in every region throughout the
world and has become recognized as one of the most common causes of waterborne
(and occasionally foodborne) illness often referred to as "Beaver Fever."
A. True B. False
153. Approximately one week after ingestion of the_________, prolonged, greasy

diarrhea, gas, stomach cramps, fatigue, and weight loss begin.
A. Intestinal flora D. Various degrees of symptoms
B. Giardia cysts E. The microaerophilic Giardia
C. Human giardiasis F. None of the Above
154. The basic biology of this ________________--including how it ravages the

digestive tract--is poorly understood.
A. Chronic case D. Disease
B. Organism E. Morphologically distinct organism
C. Parasite F. None of the Above

155. Which of the following terms uses these mitosomes in the maturation of iron-sulfur
proteins rather than in ATP synthesis as is the case in mitochondria-possessing
eukaryotes?
B. The disease mechanism E. Microaerophilic Giardia
Nature of Disease
156. Which of the following terms that appear identical to those that cause human
illness have been isolated from domestic animals and wild animals.
A. Chronic cases D. Typically, the disease
B. The organism E. Organisms
157. Which of the following terms may involve diarrhea within 1 week of ingestion of the
cyst, which is the environmental survival form and infective stage of the organism?
B. The disease mechanism E. The microaerophilic Giardia
158. Chronic cases, both those with defined _____________and those without, are
difficult to treat.
A. Immune deficiencies D. Typically, the disease
159. Which of the following terms is unknown, with some investigators reporting that the
organism produces a toxin while others are unable to confirm its existence?
160. Which of the following terms has been demonstrated inside host cells in the
duodenum, but most investigators think this is such an infrequent occurrence that it is
not responsible for disease symptoms?
A. Intestinal flora D. Typically, the disease

161. Which of the following terms of the absorptive surface of the intestine has been
proposed as a possible pathogenic mechanism, as has a synergistic relationship with
some of the intestinal flora?
B. The disease mechanism E. Mechanical obstruction

162. Which of the following terms can be excysted, cultured and encysted in vitro; new
isolates have bacterial, fungal, and viral symbionts?
A. Intestinal flora D. Typically, the disease
B. The organism E. Giardia
163. Which of the following terms have been isolated and described through analysis of
their proteins and DNA; type of strain, however, is not consistently associated with
disease severity?
A. Several strains of G. lamblia D. Various degrees of symptoms
164. Different individuals show various degrees of symptoms when infected with the
same strain, and the symptoms of an individual may vary during the?
B. Disease mechanism E. Course of the disease
Giardiasis Giardia lamblia Chapter 2

Diagnosis of Human Illness
165. Giardia lamblia is frequently diagnosed by visualizing the organism, either the
trophozoite or the cyst in stained preparations or unstained wet mounts with the aid of a
microscope.
A. True B. False
166. Which of the following terms may be concentrated by sedimentation or flotation;

however, these procedures reduce the number of recognizable organisms in the
sample?
A. Organisms D. Giardiasis
B. Infective cysts E. Recognizable organisms in the sample
C. Acute outbreaks F. None of the Above
167. Which of the following terms that detects excretory secretory products of the
organism is also available?
A. Bac-T D. Infective cysts
B. An enzyme E. An enzyme linked immunosorbant assay (ELISA)
C. Lab array F. None of the Above
Relative Frequency of Disease

168. Which of the following terms is more prevalent in children than in adults, possibly
because many individuals seem to have a lasting immunity after infection?
A. Cryptosporidium D. Infective cysts
B. An enzyme E. Trophozoite
C. Giardiasis F. None of the Above

169. Which of the following terms is implicated in 25% of the cases of gastrointestinal
disease and may be present asymptomatically, the overall incidence of infection is
estimated at 2% of the population.
A. Cryptosporidium D. Infective cysts
B. An enzyme E. Trophozoite
170. Which of the following terms appear to be common with infants and is not usually
associated with water but is related to child care and diaper changing hygiene
procedures.
A. Flagyl D. Giardiasis
B. Infective cysts E. Intestinal flora
171. According to the text, this is an example of infectious diarrhea due to

__________infection of the small intestine.
A. This organism D. Infective cysts
B. Giardia lamblia E. The small pear-shaped trophozoites
172. Which of the following terms in immunodeficient and normal individuals are
frequently refractile to drug treatment?
B. An enzyme E. Chronic cases of giardiasis
173. Which of the following terms is normally quite effective in terminating infections?
A. Flagyl D. Giardiasis
B. Infective cysts E. Recognizable organisms in the sample
Target Populations
174. Which of the following terms occurs throughout the population, although the
prevalence is higher in children than adults?
B. An enzyme E. The small pear-shaped trophozoites
175. Chronic symptomatic giardiasis is more common in adults than children.

A. True B. False
Cryptosporidiosis Cryptosporidium Chapter 3

176. Which bug or disease term describes the following symptoms, watery diarrhea and
cramps, sometimes severe, weight loss, nausea, vomiting, and fever are also possible?
A. Agammaglobulinemia D. Cryptosporidiosis
B. Toxoplasmosis E. Anti-water Infection
C. Malaise F. None of the Above

177. Cryptosporidium is a protozoan pathogen of the Phylum Apicomplexa and causes
a diarrheal illness called?
A. Cryptosporidium parvum D. Congenital agammaglobulinemia
B. Cryptosporidiosis E. Cryptosporidium
C. AIDS F. None of the Above
178. Apicomplexan pathogens include the malaria parasite Plasmodium, and

Toxoplasma, the causative agent of?
C. Toxoplasmosis F. None of the Above
179. Few people had heard of __________________, or the disease it causes,

cryptosporidiosis that until 1993, when over 400,000 people in Milwaukee became ill with
diarrhea after drinking water contaminated with the parasite.
180. Which of the following is most particularly a danger for the immunocompromised,
especially HIV-positive persons and persons with AIDS?
A. Giardia lamblia D. Cryptosporidiosis
B. Giardiasis E. Anti-water Infection
181. Immunosuppression if severe enough, can lead to chronic?

A. Cryptosporidium parvum D. Cryptosporidiosis
182. Which of the following organism/disease related terms was first identified as a
human pathogen, diagnosis was made by a biopsy of intestinal tissue?
A. C. parvum D. Congenital agammaglobulinemia
183. Staining methods were developed to detect and identify the oocysts directly from
stool samples.
A. True B. False
184. The modified acid-fast stain is traditionally used to most reliably and specifically
detect the presence of?
A. Cryptosporidium parvum D. Giardiasis
C. Cryptosporidial oocysts F. None of the Above

185. According to the text, even though persons who are taking immunosuppressive
drugs may develop chronic and/or severe _____________________, the infection
usually resolves when these drugs are decreased or stopped.
186. The following persons include child care workers; diaper-aged children who attend
child care centers; persons exposed to human feces by sexual contact; and caregivers
who might come in direct contact with feces while caring for a person infected with?
187. Which term below does not utilize an insect vector and is capable of completing its
life cycle within a single host, resulting in cyst stages which are excreted in feces and
are capable of transmission to a new host?
A. Cryptosporidium parvum D. Cryptosporidiosis
Cholera Vibrio cholerae Chapter 4

Cholera Vibrio cholerae
188. Cholera is an infection of the small intestine that causes a large amount of watery
diarrhea.
A. True B. False
189. According to the text, Cholera is an infection in the small intestine caused by?
A. Amoebiasis D. Rapid dehydration and electrolyte imbalance
B. Cholera E. Diarrheal disease
C. The bacterium Vibrio cholerae F. None of the Above
190. Which of the following terms is protected from the severe effects of cholera
because they don't lose water as quickly?
A. Blood types D. Carriers of the cystic fibrosis gene
B. Thin people E. Carriers of rabies
C. Antibacterial drugs F. None of the Above
191. Which of the following terms appears in a community it is essential to ensure three
things: hygienic disposal of human feces, an adequate supply of safe drinking water, and
good food hygiene?
A. Cryptosporidium parvum D. El Tor
C. Cholera F. None of the Above
192. Because of the severity of the diarrhea and vomiting can lead to rapid dehydration
and electrolyte imbalance, and?
A. Amoebiasis D. Death

193. To shorten its duration and severity, antibacterial drugs are beneficial in those
with?
C. Severe disease F. None of the Above
194. Which of the following organism/disease related term has been very rare in
industrialized nations for the last 100 years?
A. Amoebiasis D. Entamoeba histolytica
B. Cholera E. Cystic fibrosis gene
C. Mexicana cholera F. None of the Above
195. Which of the following organism/disease related term is the most feared epidemic
diarrheal disease because of its severity?
B. Vibrio cholerae E. Cystic fibrosis
C. Bacterial meningitis F. None of the Above
196. Which of the following organism/disease related term is a disease of the

gastrointestinal tract caused by the Vibrio cholerae bacterium and is also known as?
A. Amoebic cholera D. European cholera
B. Amoebiasis E. Asiatic cholera
197. Cholera has been found in only two other animal populations: shellfish and
plankton.
A. True B. False
198. Which of the following terms is rarely spread directly from person to person?
199. Cholera has two strains, toxic and nontoxic.

A. True B. False
200. Which of the following organism/disease related term was prevalent in the 1800s
but has been virtually eliminated by modern sewage and water treatment systems?
A. Amoebic cholera D. European cholera
B. Cholera E. Asiatic cholera
201. Genetic research has determined that a person's susceptibility to cholera and other
diarrheas) is affected by their blood type.
A. True B. False
202. Those with type A blood is the most susceptible.

A. True B. False

203. Those with type O are the most resistant, virtually immune. Between these two
extremes are the O and O negative blood types.
A. True B. False
204. The mode of transmission of this disease by water was proven in 1849 by John
Snow.
205. Cholera is typically transmitted by either contaminated food or water. With seafood
being the usual cause, while in the developing world it is more often water.
A. True B. False
Cholera Treatment
206. When consumed, most bacteria do not survive the?
A. Stomach acid D. Resistance
B. Lack of nutrients E. Antibiotic treatments
207. During the passage through the stomach, few surviving bacteria conserve their
energy and stored nutrients by shutting down much?
A. Life support D. Resistance
B. Protein production E. Antibiotic treatments
C. Reproduction F. None of the Above
208. Surviving Cholera Bacteria exit the stomach and reach the small intestine; they
need to propel themselves through the thick mucus that lines the small intestine to get to
the intestinal walls, where they can thrive.
A. True B. False
209. V. cholerae bacteria start up production of the hollow cylindrical protein flagellin to
make flagella, the cork-screw helical fibers they rotate to propel themselves through the
mucus of the small intestine.
A. True B. False
210. If Cholera bacteria reach the intestinal wall, they will no longer need?
A. Lamblia D. Cyst
B. Shell E. Flagella
C. Case F. None of the Above
211. On reaching the intestinal wall, V. cholerae start producing the Antibiotic
treatments that give the infected person a watery diarrhea.
A. True B. False
212. This carries the multiplying new generations of V. cholerae bacteria out into the
drinking water of the next host if even if proper sanitation measures are in place.
A. True B. False

213. Which of the following terms can be administered for one to three days shorten the
course of the disease and reduce the severity of the symptoms?
A. Verotoxin D. Resistance
B. Antibiotic treatments E. Aspirin
214. If sufficient hydration is maintained, people will recover without drugs.

A. True B. False
215. Which of the following terms is typically used first line, although some strains of V.
cholerae that have shown resistance?
A. Verotoxin D. Resistance
B. Doxycycline E. Aspirin
216. Rapid diagnostic assay methods are available for the identification of?
A. Cholera bacteria-resistant cases D. Resistance
B. Multiple drug-resistant cases E. Antibiotic treatments
217. Cholera remains a pandemic in many areas of the world.

A. True B. False
218. According to the text, little is known about the mechanisms behind the spread of
cholera, this has not led to a full understanding of what makes cholera outbreaks happen
in some places and not others.
A. True B. False
219. The term Cholera morbus was used to describe both nonepidemic cholera and
other gastrointestinal diseases (sometimes epidemic) that resembled cholera.
A. True B. False
El Tor
220. El Tor strain has a low degree of "epidemic virulence," allowing it to spread across
the world as previous strains have done. First, the ratio of cases to carriers is much less
than in Giardia lamblia due to classic biotypes (1: 30-100 for El Tor vs. 1: 2 - 4 for
"classic" biotypes).
A. True B. False
Legionnaires' Disease Legionella Chapter 5

221. The first discovery of bacteria was from the ________________that came in 1976
when an outbreak of pneumonia at an American Legion convention led to 29 deaths.
A. Legionnaire’s disease D. Genus Legionella
B. Legionella pneumophila E. Legionella species
C. Legionella bacteria F. None of the Above
222. The causative agent of Legionnaires' Disease, would come to be known as

Legionella pneumophila, was isolated and given its own genus.
A. True B. False

223. Which of the terms are classified in this genus are Gram-negative bacteria that are
considered intracellular parasites?
A. Legionnaire’s disease D. Organisms
224. Which of the terms is caused most commonly by the inhalation of small droplets of
water or fine aerosol containing Legionella bacteria?
A. Legionnaire’s disease D. Legionella
225. Which of the terms are naturally found in environmental water sources such as
rivers, lakes and ponds and may colonize manmade water systems?
226. Which of the terms is responsible for approximately 90% of infections?

227. Most Legionnaire’s disease cases are caused by__________________, serogroup

1. Legionella species are small (0.3 to 0.9 μm)
A. Legionnaire’s disease D. L. pneumophila
Chlorine Dioxide
228. Which term provides good Giardia and virus protection but its use is limited by the
restriction on the maximum residual of 0.5 mg/L ClO2/chlorite/chlorate allowed in
finished water?
A. Dry sodium chlorite D. Ammonia residual(s)
B. Chlorine dioxide E. Free and/or combined chlorine
C. Chlorinated byproducts F. None of the Above
229. Chlorine dioxide may be used for either taste or odor control or as a?
A. Chloramine D. Chlorine dioxide
B. T10 value E. Pre-disinfectant
C. Free chlorine F. None of the Above
Escherichia Coli Chapter 6

E-Coli Section – Some information may be in the Appendix.
230. Escherichia coli. There are several pathogenic strains of Escherichia coli, which
are classified under enterovirulent E. coli.
A. True B. False
231. Escherichia coli. are enterohemorrhagic, enteroinvasive, enterotoxigenic,

enteropathogenic, and enteroaggregative.
A. True B. False

232. Escherichia coli. In its most severe form, it can cause?
A. Hemorrhagic colitis D. Pseudomonas
B. Escherichia coli O157:H7 E. Salmonellosis
C. Beaver fever F. None of the Above
233. Prevention strategies for E. coli O157:H7 include _____________, halogenation of

water, or boiling water for one minute.
A. Primary protection D. Eliminating snails with a molluscicide
B. Source protection E. Backflow prevention
C. Sodium chlorite F. None of the Above
234. What is the bacterial disease caused by the Salmonella species that causes
diarrheal illness?
A. Beaver fever D. Pseudomonas
B. Escherichia coli O157:H7 E. Salmonellosis
C. Bacteria F. None of the Above
235. Prevention strategies for Salmonella include source protection, halogenation of

water, and also?
A. KNMO4 D. Eliminating snails with a molluscicide
B. Source protection E. Boiling water for one minute
C. Chlorine dioxide F. None of the Above
Waterborne Pathogens and Disease Review

How Diseases Are Transmitted.
236. Waterborne pathogens are primarily spread by the?
A. Fecal-oral, or feces-to-mouth, route D. Influenza route
B. Dermal to fecal route E. Waterborne mishaps
C. Oral to fecal route F. None of the Above
237. When infected humans or animals pass the bacteria, viruses, and
_________________in their stool, pathogens may get into water and spread disease.
A. Fecal Coliform and E coli D. Cryptosporidiosis
B. Protozoa E. Bioslime
C. Macroorganisms F. None of the Above
238. For another person to become infected, he or she must take that pathogen in
through the mouth.
A. True B. False
239. This term means when in nature it is different from other types of pathogens such
as the viruses that cause influenza (the flu) or the bacteria that cause tuberculosis.
A. Fecal Coliform and E coli D. Waterborne Pathogen(s)
C. Microorganism(s) F. None of the Above
240. According to the text, __________________are spread by secretions that are

coughed or sneezed into the air by an infected person.
A. Fecal Coliform and E coli D. Influenza virus and tuberculosis bacteria

Bacterial Diseases
241. Bacterial diseases that can be transmitted through water, Cholera, Legionellosis,
salmonellosis, shigellosis, and?
A. Shigellosis D. Campylobacteriosis
B. Cysts E. HIV
C. Hepatitis A F. None of the Above
242. Which of the following organism/disease related term polio, and viral gastroenteritis
(Norwalk agent) and this one are other viral diseases that can be transmitted through
water?
A. Pathogens D. Foodborne or waterborne illnesses
B. Bacterial diseases E. Amebiasis
C. Aseptic meningitis F. None of the Above
243. According to the text, few viruses in drinking water can be inactivated by chlorine
or other disinfectants.
A. True B. False
244. A susceptible person must drink the water that contains the pathogen in order for
illness (disease) to occur.
A. True B. False
245. Which of the following organism/disease term will is the most common diarrheal
illness caused by bacteria?
A. Aseptic meningitis D. Giardia or Cryptosporidium
B. Campylobacteriosis E. Tuberculosis bacteria
Amebiasis– Some information may be in the Appendix.

246. Amebiasis is an infection of the intestines caused by?
A. Pathogens D. Foodborne or waterborne illnesses
B. Bacterial diseases E. The parasite Entamoeba histolytica
C. Norwalk agent F. None of the Above
247. Amoebiasis, or Amebiasis, refers to infection caused by?

A. Amoebiasis D. The amoeba Entamoeba histolytica
248. Which of the following organism/disease related term is usually transmitted by the
fecal-oral route, but it can also be transmitted indirectly through contact with dirty hands
or objects as well as by anal-oral contact?

249. Any non-encysted amoebae, or__________________________, die quickly after
leaving the body?
A. Amoebiasis D. Electrolytes
C. Trophozoites F. None of the Above
250. Which of the following organism/disease term that may or may not be symptomatic
and can remain latent in an infected person for several years?
A. Gastrointestinal infection D. Rapid dehydration and electrolyte imbalance
251. Which of the following organism/disease term can live in the large intestine (colon)
without causing disease?
Hepatitis Section
252. Which of the following hepatitis types affects only those who also have hepatitis B,
and hepatitis E is extremely rare in the United States?
A. Type B D. Type D
B. Type A E. Type E hepatitis
C. Type C F. None of the Above
253. Which of the following hepatitis can be passed through contact with infected blood,
contaminated needles, or by sexual contact with an HIV-infected person?
A. Type A hepatitis D. Type D hepatitis
B. Type B hepatitis E. Type E hepatitis
C. Type C hepatitis F. None of the Above
254. Which of the following hepatitis is most likely to be transmitted in feces, through
oral contact, or in water that's been contaminated?
A. Type B hepatitis D. Type D hepatitis
B. Type A hepatitis E. Type E hepatitis
255. Which of the following hepatitis is contracted through anal-oral contact, by coming
in contact with the feces of someone with it, or by eating or drinking hepatitis
contaminated food or water?
256. Which of the following hepatitis can be contracted from infected blood, seminal
fluid, vaginal secretions, or contaminated drug needles, including tattoo or body-piercing
equipment.

257. Which of the following hepatitis is not easily spread through sex?
A. Type B hepatitis D. Type D hepatitis
B. Type A hepatitis E. Type E hepatitis
Shigella dysenteriae– Some information may be in the Appendix.

258. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E.
coli O157:H7. Both Shiga toxin and verotoxin are associated with causing hemolytic
uremic syndrome.
A. True B. False
259. Which term invades the host through epithelial cells of the large intestine?
A. Bacillary dysentery D. Shigella
B. Shigellae E. Verotoxin
C. S. dysenteriae F. None of the Above
260. Which term are diarrhea, fever, nausea, vomiting, stomach cramps, and straining
to have a bowel movement?
A. Bacillary dysentery D. Shigella infection
261. Shigella dysenteriae is a species of the ball-shaped bacterial genus Shigella.

A. True B. False
262. Which term can cause shigellosis (bacillary dysentery)?

A. Bacillary dysentery D. Infection
B. Shigella E. Verotoxin
263. Which term are Gram-negative, non-spore-forming, facultatively anaerobic, non-

motile bacteria?
264. According to the text, S. dysenteriae, spread by contaminated water and food,
causes the most severe dysentery because of its potent and deadly________________,
but other species may also be dysentery agents.
A. Bacillary dysentery D. Shiga toxin
265. Which term is typically via ingestion (fecal–oral contamination); depending on age
and condition of the host as few as ten bacterial cells can be enough to cause an
infection?

266. Which term causes dysentery that result in the destruction of the epithelial cells of
the intestinal mucosa in the cecum and rectum?
267. Which term usually last for several days, but can last for weeks. Shigella is
implicated as one of the pathogenic causes of reactive arthritis worldwide?
C. Symptoms F. None of the Above
Shigellosis
268. Which term is an infectious disease caused by a group of bacteria called Shigella?
A. Typhoid fever D. Gram-negative short bacillus
B. Gastric fever E. A positive reaction
C. Shigellosis F. None of the Above
269. Those who are infected with Shigella develop diarrhea, fever, and stomach cramps
starting a week or two after they are exposed to the bacteria. The diarrhea is often
watery.
A. True B. False
270. Which term usually resolves in 5 to 7 days?

271. Persons with shigellosis in the United States often require hospitalization.
A. True B. False
272. According to the text, some persons who are infected may have no symptoms at
all, but may still pass the _______________to others.
B. Gastric fever E. Shigella bacteria
273. Which term is an acute bacterial infection of the lining of the intestines?
Typhoid
274. Typhoid fever, also known as Typhoid, is a common worldwide bacterial disease,
transmitted by the ingestion of food or water contaminated with the feces of an infected
person, which contain the bacterium Salmonella typhi, Serovar Typhi.
A. True B. False
275. Salmonella typhi is a Gram-negative short bacillus that is motile due to its
peritrichous flagella.
A. True B. False

276. Salmonella typhi grows best at 37°C / 98.6°F – human body temperature.
A. True B. False
277. This fever received various names, such as gastric fever, _______________,
infantile remittent fever, slow fever, nervous fever, pythogenic fever, etc.
C. Abdominal typhus F. None of the Above
278. Typhoid fever is unrelated to?

A. Typhoid flu D. Typhus
B. Gastric fever E. Typhoid hurricane
279. Which term is divided into 4 individual stages, each lasting approximately 1 week.
In the 1st week, the temperature rises slowly and fever fluctuations are seen with relative
bradycardia, malaise, headache, and cough?
280. According to the text, there is leukopenia, with eosinopenia and relative
lymphocytosis, a positive reaction and blood cultures are positive for?
A. Typhoid fever D. Salmonella typhi or paratyphi
How is typhoid fever spread?

281. Salmonella Typhi lives only in humans. Persons with typhoid fever carry the
bacteria in their bloodstream and intestinal tract.
A. True B. False
282. Typhoid fever is spread through food or drink beverages that have been handled
by a person who is shedding ______________________ or if sewage contaminated with
Salmonella Typhi bacteria gets into the water you use for drinking or washing food.
A. Typhoid bugs D. Salmonella Typhi
283. Which term when eaten or drunk, they multiply and spread into the bloodstream,
the body reacts with fever and other signs and symptoms?
B. Gastric fever E. Salmonella Typhi

EPA Regulations Chapter 8
Disinfection Rule Review
284. Chlorine is the most widely used water disinfectant due to its effectiveness and
cost. Using chlorine as a drinking water disinfectant has prevented millions of water
borne diseases, such as typhoid, cholera, dysentery, and diarrhea. Most states require
community water systems to use chlorination.
A. True B. False
285. These compounds are called disinfection by-products (DBPs). All disinfectants
form DBPs in one of two reactions: Chorine and chlorine-based compounds (halogens)
react with organics in water causing the chlorine atom to substitute other atoms resulting
in?
A. Chlorine D. Halogenated by-products
B. Organic sulfide(s) E. HOCl
C. Calcium carbonate F. None of the Above
286. Oxidation reactions, where chlorine oxidizes __________present in water.

Secondary by-products are also formed when multiple disinfectants are used.
A. Carbon D. Chorine and chlorine-based compounds (halogens)
B. Surface water E. Secondary by-products
C. Compounds F. None of the Above
287. Which of the following terms requires systems using public water supplies from
either surface water or groundwater under the direct influence of surface water to
disinfect?
A. TTHM and HAA5 Rule D. Disinfection byproducts (DBPs) Rule
B. DBP MCLs Rule E. Surface Water Treatment Rule (SWTR)
C. A community water system (CWS) F. None of the Above
288. Since some disinfectants produce chemical by-products, the dual objective of
disinfection is to provide the required level of organism destruction and remain within the
maximum contaminant level (MCL) for the SWTR disinfection set by EPA.
A. True B. False
289. At this time, an MCL is set for only _______________, and proposed for additional
disinfection byproducts.
B. DBP MCLs Rule E. Total Trihalomethanes
290. Which of the following rules apply to all community and non-community water
systems using a disinfectant such as chlorine, chloramines, ozone and chlorine dioxide?
B. DBP MCLs Rule E. Disinfectants and Disinfection Byproducts (DBP)
C. A community water system F. None of the Above

291. The Long Term 2 Enhanced Surface Water Treatment Rule (LT2) rule applies to all
water systems using _________________under the influence of a surface water, as well
as groundwater/surface water blends.
A. Surface water, groundwater D. Disinfection byproducts (DBPs) Rule
B. DBP MCLs Rule E. Total Trihalomethanes
Public Health Concerns

292. While disinfectants are effective in controlling many microorganisms, they react
with natural organic and inorganic matter in source water and distribution systems to
form?
A. DBPs D. Classes of DBPs
B. Chlorine and chloramine E. Ultraviolet light
C. Stage 2 DBPR F. None of the Above
293. Which term is used to express (e.g., chlorite, bromodichloromethane, and certain
haloacetic acids) have also been shown to cause adverse reproductive or
developmental effects in laboratory animals?
294. Several epidemiology studies have suggested a weak association between certain
cancers or reproductive and developmental effects, and exposure to chlorinated surface
water.
A. True B. False
295. More than 200 million people consume water that has been disinfected. Because of
the large population exposed, health risks associated with ____________, even if small,
need to be taken seriously.
296. Which Rule and Disinfection Byproduct Rule updates and supersedes the 1979
regulations for total trihalomethanes? In addition, it will reduce exposure to three
disinfectants and many disinfection byproducts.
A. DBPs D. Stage 3 Disinfectant and Disinfection Byproduct
B. The Stage 1 Disinfectant E. The LT2 requirements
C. SDWA in 1996 F. None of the Above
Stage 2 DBP Rule Federal Register Notices

297. Which Rule is one part of the Microbial and Disinfection Byproducts Rules
(MDBPs), which are a set of interrelated regulations that address risks from microbial
pathogens and disinfectants/disinfection byproducts?
A. Groundwater Rule D. Long Term 2 Enhanced Surface Water Treatment
B. Compliance E. Interim Enhanced Surface Water Treatment
C. The Stage 2 DBP F. None of the Above

298. Which Rule focuses on public health protection by limiting exposure to DBPs,
specifically total trihalomethanes (TTHM) and five haloacetic acids (HAA5), which can
form in water through disinfectants used to control microbial pathogens?
A. Stage 2 DBPR D. Long Term 2 Enhanced Surface Water Treatment
B. DBP exposure E. Traditional disinfection practices
C. The Stage 2 DBP F. None of the Above
299. This rule will apply to all community water systems and nontransient non-
community water systems that add a primary or residual disinfectant other than
___________or deliver water that has been disinfected by a primary or residual
disinfectant other than UV.
A. Ultraviolet (UV) light D. UV source
B. The open-channel system E. UV radiation
C. UV rather than ozone F. None of the Above
300. Which of the following rules has been highly effective in protecting public health
and has also evolved to respond to new and emerging threats to safe drinking water?
A. Stage 2 DBPR D. Long Term 2 Enhanced Surface Water Treatment Rule
B. DBP exposure E. Safe Drinking Water Act (SDWA)
C. The Stage 2 DBP rule F. None of the Above
301. There are specific microbial pathogens, such as__________________, which can
cause illness, and are highly resistant to traditional disinfection practices.
A. Enteric virus(es) D. C. perfringens
B. Oocyst(s) E. E. coli host culture
C. Cryptosporidium F. None of the Above
302. Amendments to the SDWA in 1996 require EPA to develop rules to balance the
risks between microbial pathogens and disinfection byproducts (DBPs).
A. True B. False
303. The Stage 1 Disinfectants and Disinfection Byproducts Rule and__________,

promulgated in December 1998, were the first phase in a rulemaking strategy required
by Congress as part of the 1996 Amendments to the Safe Drinking Water Act.
A. Major health advances D. Amendments to the SDWA in 1996
B. The Stage 2 DBPR E. Interim Enhanced Surface Water Treatment Rule
C. This final rule F. None of the Above
304. The Stage 2 Disinfectants and Disinfection Byproducts Rule (Stage 2 DBPR)
builds upon the ____________to address higher risk public water systems for protection
measures beyond those required for existing regulations.
305. Which Rule and the Long Term 2 Enhanced Surface Water Treatment Rule are the
second phase of rules required by Congress?
A. Major public health advances D. Amendments to the SDWA in 1996
B. The Stage 2 DBPR E. Primary or residual disinfectant

306. Which Rule will reduce potential cancer and reproductive and developmental
health risks from disinfection byproducts (DBPs) in drinking water, which form when
disinfectants are used to control microbial pathogens?
A. Stage 3 DBPR D. Long Term 2 Enhanced Surface Water
C. Stage 2 Disinfection Byproducts F. None of the Above
307. Which Rule strengthens public health protection for customers by tightening
compliance monitoring requirements for two groups of DBPs, trihalomethanes (TTHM)
and haloacetic acids (HAA5)?
C. Stage 2 Disinfection Byproducts F. None of the Above
308. Which Rule targets systems with the greatest risk and builds incrementally on
existing rules. This regulation will reduce DBP exposure and related potential health
risks and provide more equitable public health protection?
B. Stage 2 DBPR E. Stage 4 DBPR
309. Which Rule is being promulgated simultaneously with the Long Term 2 Enhanced
Surface Water Treatment Rule to address concerns about risk tradeoffs between
pathogens and DBPs?
What does the rule require?

310. Under which Rule, systems will conduct an evaluation of their distribution systems,
known as an Initial Distribution System Evaluation (IDSE), to identify the locations with
high disinfection byproduct concentrations?
311. Compliance with the maximum contaminant levels for two groups of disinfection
byproducts (TTHM and HAA5), referred to as the?
A. TTHM and HAA5 D. Disinfection byproducts (DBPs)
B. DBP MCLs E. Trihalomethanes and haloacetic acids
C. Locational running annual average (LRAA)) F. None of the Above
312. Which Rule also requires each system to determine if they have exceeded an
operational evaluation level, which is identified using their compliance monitoring
results?

313. A system that exceeds an operational evaluation level is required to review their
operational practices and submit a report to their state that identifies actions that may be
taken to mitigate future high?
A. TTHM5 and HTAA5 D. UV
B. Halos E. Amounts of rainfall
C. DBP levels F. None of the Above
Who must comply with the rule?

314. Entities potentially regulated by the _______________ are community and
nontransient noncommunity water systems that produce and/or deliver water that is
treated with a primary or residual disinfectant other than ultraviolet light.
A. DBPs from chlorination D. Classes of DBPs
B. Chlorine and chloramine E. TTHM and HAA5
315. Which system is a public water system that serves year-round residents of a
community, subdivision, or mobile home park that has at least 15 service connections or
an average of at least 25 residents.
A. NTNCWS D. Trailer park
B. A non-community water system E. A nontransient water system
316. Which system is a water system that serves at least 25 of the same people more
than six months of the year, but not as primary residence, such as schools, businesses,
and day care facilities?
A. Trailer park D. A nontransient non-community water system (NTNCWS)
B. A non-community water system E. A nontransient water system
What are Disinfection Byproducts (DBPs)?

317. Which term forms when disinfectants used to treat drinking water react with
naturally occurring materials in the water (e.g., decomposing plant material)?
A. Disinfectants D. Disinfection byproducts (DBPs)
B. DBLs E. Sodium Thiosulfate
C. Humic F. None of the Above
318. Total trihalomethanes (TTHM - chloroform, bromoform, bromodichloromethane,

and dibromochloromethane) and haloacetic acids (HAA5 - monochloro-, dichloro-,
trichloro-, monobromo-, dibromo-) are widely occurring _____________ formed during
disinfection with chlorine and chloramine.
A. Sodium Thiosufate D. Classes of DBPs
B. Chlorine and chloramine E. Trihalomethanes and haloacetic acids
319. Which term in drinking water can change from day to day, depending on the
season, water temperature, amount of disinfectant added, the amount of plant material
in the water, and a variety of other factors?
A. Thiols D. Classes of DBPs
B. Chlorine and chloramine E. Trihalomethanes and haloacetic acids

Are THMs and HAAs the only disinfection byproducts?
320. The presence of this term is representative of the occurrence of many other
chlorination DBPs; thus, a reduction in the these generally indicates a reduction of DBPs
from chlorination.
A. DBPs from chlorination D. Classes of DBPs
B. Chlorine and chloramine E. TTHM and HAA5
Stage 2 DBP Rule Federal Register Notices

321. Chlorine and its _______________are neutrally charged and therefore easily
penetrate the negatively charged surface of pathogens.
A. Halogen D. Hydrolysis product hypochlorous acid
B. Water chlorination E. Hypochlorous acid
C. Chlorine as a disinfectant F. None of the Above
322. There are specific microbial pathogens, such as ____________, which can cause
illness and is resistant to traditional disinfection practices.
A. Cryptosporidium D. Emerging threats to safe drinking water
B. Sodium hypochlorite E. Hypochlorous acid (HOCl), and hydrochloric acid (HCl)
C. Bromoform F. None of the Above
Water Sampling and Laboratory Procedures Chapter 9

Arsenic
323. Long-term exposure of this compound/element/substance in drinking water to a
variety of cancers in humans.
A. Arsenic D. THHMMS
B. Copper E. Silica
C. Basalt F. None of the Above
324. The EPA set a standard limit or the amount of compound/element/substance in

drinking water to 10 ppb.
A. Arsenic D. Copper
B. Trihalomethanes E. Disinfection byproducts (DBPs)
C. Disinfection F. None of the Above
325. This compound/element/substance is a chemical that occurs naturally in the earth's

crust. When rocks, minerals, and soil erode, they release this compound/ element/
substance into water supplies.
A. Arsenic D. Basalt
B. Trihalomethanes E. Granite
C. Disinfection byproducts F. None of the Above
ICR
326. The EPA has collected data required by the Information Collection Rule (ICR) to
support future regulation of Microbial contaminants, disinfectants, and disinfection
byproducts.
A. True B. False

327. The rule is intended to provide EPA with information on chemical byproducts that
form when disinfectants used for microbial control react with chemicals already present
in source water Disease-causing microorganisms, including Cryptosporidium; and
engineering data to control these contaminants.
A. True B. False
Disinfection Byproduct Regulations

328. Which compound/element/substance are a group of chemicals that are formed
along with other disinfection byproducts when chlorine or other disinfectants are used?
A. Disinfectant residual D. Giardia and viruses
B. Chlorite E. Disinfection By-Products (DBPs)
C. Haloacetic Acids (HAA5) F. None of the Above
329. Which compound/element/substance is a chemical that is formed when ozone,

used to disinfect drinking water, reacts with naturally occurring bromide found in source
water?
A. Bromate D. From the results of coliform testing
B. Counter pathogens E. Bacteria, Virus and Intestinal parasites
C. Monobromoacetic acid F. None of the Above
330. Trihalomethanes are regulated at a maximum allowable annual average level of

100 ppb for water systems serving more than 10,000 people under which of the following
rules?
A. Cryptosporidium D. Total Trihalomethane
B. Trihalomethanes E. Stage 1 Disinfectant/Disinfection Byproduct
C. Disinfection byproduct F. None of the Above
331. Which rule standard became effective for trihalomethanes and other disinfection
byproducts in December 2001 for large surface water public water systems?
B. Trihalomethanes E. Stage 1 Disinfectant/Disinfection Byproduct
C. Disinfection byproduct F. None of the Above
332. Which compound/element/substance are formed when disinfectants used in water

treatment plants react with bromide and/or natural organic matter (i.e., decaying
vegetation) present in the source water?
C. Chlorine byproduct F. None of the Above
333. According to the text, different disinfectants produce different types or amounts of?
C. Chlorine byproduct F. None of the Above
334. Which rule standards have been established have been identified in drinking
water, including trihalomethanes, haloacetic acids, bromate, and chlorite?
A. Cryptosporidium Rule D. Total Trihalomethane Rule
B. Trihalomethanes Rule E. Disinfection byproducts (DBPs)
C. Acceptable standard F. None of the Above

335. Which compound/element/substance are chloroform, bromodichloromethane,
dibromochloromethane, and bromoform?
A. Cryptosporidium D. Total HAAA5s
B. Trihalomethanes E. Maximum Contaminant Levels MCLs
C. HAAs F. None of the Above
Microbial Regulations
336. One of the key regulations developed and implemented by the United States
Environmental Protection Agency to counter pathogens in drinking water is the Surface
Water Treatment Rule requires that a public water system, using surface water as its
source, have sufficient treatment to reduce the source water concentration of Giardia
and viruses by at least 99.9% and 99.99%, respectively.
A. True B. False
337. Which rule specifies treatment criteria to assure that these performance
requirements are met; they include turbidity limits, disinfectant residual, and disinfectant
contact time conditions?
A. Long Term 1 Rule D. Surface Water Treatment Rule
B. Maximum Contaminant Level Goal (MCLG) E. Interim Enhanced Surface Water
C. Stage 1 Byproducts Rule F. None of the Above
338. Which rule was established to maintain control of pathogens while systems lower
disinfection byproduct levels to comply with the Stage 1 Disinfectants/Disinfection
Byproducts Rule and to control Cryptosporidium?
A. Long Term 1 Enhanced Surface Water Treatment Rule
B. Maximum Contaminant Level Goal (MCLG)
C. Stage 1 Disinfectants/Disinfection Byproducts Rule
D. Surface Water Treatment Rule
E. Interim Enhanced Surface Water Treatment Rule
F. None of the Above
339. The EPA established a MCL of 0.0010 for all public water systems and a 99%
removal requirement for Cryptosporidium in filtered public water systems that serve at
least 100,000 people. The new rule will tighten turbidity standards by December 2001.
A. True B. False
340. Color is an indicator of the physical removal of particulates, including pathogens.

A. True B. False
Bacteriological Monitoring Section

341. Which of the following are usually harmless, occur in high densities in their natural
environment and are easily cultured in relatively simple bacteriological media?
A. Indicator bacteria D. Microbiological analysis
B. Bacteria tests E. Presence of an indicator
C. Contaminate F. None of the Above
342. Indicators in common use today for routine monitoring of drinking water include
total coliforms, fecal coliforms, and ______________.
A. Sample container D. Escherichia coli (E. coli)
B. Bacteria tests E. Iron bacteria
C. Coliform bacteria F. None of the Above

343. According to the text, the routine microbiological analysis of your water is for?
A. Indicator bacteria D. Coliform bacteria
C. Contamination F. None of the Above
344. Which of the following terms is used as an indicator organism to determine the
biological quality of your water?
A. Microbiological analysis D. Escherichia coli (E. coli)
B. Bac-T E. Presence of an indicator
345. The presence of an indicator or ________________in your drinking water is an

important health concern.
B. Pathogenic bacteria E. Presence of an indicator
346. Which of the following terms is used to signal possible fecal contamination, and
therefore, the potential presence of pathogens?
B. Pathogenic bacteria E. Presence of an indicator
Bacteria Sampling
347. Water samples for this process must always be collected in a sterile container.
A. Indicators D. pH analysis
C. Contamination F. None of the Above
348. Refrigerate the sample and transport it to the testing laboratory within eight hours
(in an ice chest). Many labs will accept bacteria samples on Friday. Mailing Indicator
bacteria is not recommended because laboratory analysis results are not as reliable.
A. True B. False
349. Which bug forms an obvious slime on the inside of pipes and fixtures. A water test
is not needed for identification. Check for a reddish-brown slime inside a toilet tank or
where water stands for several days.
A. Colonies D. Escherichia coli (E. coli)
B. Algae E. Iron bacteria
350. Which of the following are common in the environment and are generally not
harmful, but the presence of these bacteria in drinking water is usually a result of a
problem with the treatment system or the pipes which distribute water, and indicates that
the water may be contaminated with germs that can cause disease?
A. Diseases D. Escherichia coli (E. coli)
B. Germs E. Iron bacteria

Laboratory Procedures
351. The laboratory may perform the _______________ in one of four methods
approved by the U.S. EPA and your local environmental or health division.
A. Colilert D. Total coliform analysis
B. Coliform E. Pathogen test
C. Sample time F. None of the Above
Methods
352. The MMO-MUG test, a product marketed as ____________, is the most common.
The sample results will be reported by the laboratories as simply coliforms present or
absent.
B. Coliform E. Pathogen media
C. Sample stuff F. None of the Above
353. If coliforms are present, the laboratory will analyze the sample further to determine
if these are ________ and __________and report their presence or absence.
A. Colilert, E. coli D. Total coliform analysis, Pathogens
B. Coliforms, E. coli E. Pathogens, Total coliform analysis
C. Fecal coliforms, E. coli F. None of the Above
Types of Water Samples

354. It is important to properly identify the type of ____________ you are collecting.
B. Coliforms E. Pathogens
C. Sample F. None of the Above
The three (3) types of samples are:

355. Samples collected following a coliform present’ routine sample. The number of
repeat samples to be collected is based on the number of ____________ samples you
normally collect.
A. Repeat D. Total coliform analysis
B. Special E. Routine
C. QA QC F. None of the Above
356. What type of samples can be collected for other reasons? Examples would be a
sample collected after repairs to the system.
357. What type of samples can be collected on a routine basis to monitor for
contamination? Collection should be in accordance with an approved sampling plan.

Repeat Sampling
358. If a _________________ is total coliform or fecal coliform present, a set of repeat
samples must be collected within 24 hours after being notified by the laboratory.
A. MCL compliance D. Original sampling location
B. Distribution system E. Repeat sample(s)
C. Routine sample F. None of the Above
The follow-up for repeat sampling is:

359. If only one _________________per month or quarter is required, four (4) repeat
samples must be collected.
A. Special Sample D. Coliform present
B. Routine sample E. Original sampling location
C. Repeat sample(s) F. None of the Above
360. For systems collecting two (2) or more routine samples per month, three (3)
____________ must be collected.
A. Compliance sample D. QA/QC Split
B. Distribution sample E. Repeat sample(s)
361. Repeat samples must be collected from: Within five (5) service connections
upstream from the?
A. MCL compliance D. Original sampling location
B. Distribution system E. Repeat sample(s)
362. Repeat samples must be collected from: Within five (5) service connections
downstream from the?
363. Repeat samples must be collected from: If the system has only one service
connection, the _________________ must be collected from the same sampling location
over a four-day period or on the same day.
364. Repeat samples must be collected from: All ______________ are included in the
MCL compliance calculation.
Sampling Procedures
365. This must be followed and all operating staff must be clear on how to follow the
sampling plan.
A. Seal individual samples D. Sample siting plan
B. Chain of custody E. Positive for total coliform
C. Distribution system F. None of the Above

366. Staff must be aware of how often sampling must be done, the _______________to
be used for collecting the samples.
A. Multiple sources D. Proper procedures and sampling containers
B. Sample siting plan E. Sampling containers
C. Total coliform F. None of the Above
367. According to the text, proper procedures must be followed for repeat sampling
whenever a routine sample result is?
Chain of Custody Procedures

368. If you have physical possession of a sample, have it in view, or have physically
secured it to prevent tampering then it is defined as being in “custody."
A. True B. False
369. Which of the following terms begins when the sample containers are obtained from
the laboratory?
A. Multiple sources D. Chain of custody record
370. Each custody sample requires a _____ record and may require a seal. If you do
not seal individual samples, then seal the containers in which the samples are shipped.
371. The sample is physical evidence, ____________ procedures are used to maintain
and document sample possession from the time the sample is collected until it is
introduced as evidence.
A. Multiple sources D. TCR
B. Sample siting plan E. Chain of custody
372. If both parties involved in the transfer must sign, date and note the time on the
chain of custody record, this is known as?
A. Multiple sources D. Samples transfer possession
373. The recipient will then attach the _______________showing the transfer dates and
times to the custody sheets.
C. Shipping invoices F. None of the Above

374. If the samples are delivered to after-hours night drop-off boxes, the custody record
should note such __________________and be locked with the sealed samples inside
sealed boxes.
A. Multiple sources D. TCR
B. Sample siting plan E. A transfer
Heterotrophic Plate Count HPC

375. Heterotrophic Plate Count (HPC) --- formerly known as the Standard plate count, is
a procedure for estimating the number of live heterotrophic bacteria and measuring
changes during water treatment and distribution in water or in swimming pools.
A. True B. False
376. Colonies may arise from pairs, chains, clusters, or single cells, all of which are
included in the term?
A. Coliform bacteria units D. HPC units
B. MCLs units E. Colony-forming units
C. Standards F. None of the Above
Spread Plate Method

377. During this method, colonies are on the __________where they can be
distinguished readily from particles and bubbles.
A. Agar surface D. Bottom
B. Surface growth area E. Material
C. Top F. None of the Above
378. According to the text, during the Spread Plate Method, colonies can be transferred
quickly, and __________easily can be discerned and compared to published
descriptions.
A. Colonies grow D. Heterotrophic organisms will grow
B. Surface growth E. Colony morphology
C. Low counts F. None of the Above
Membrane Filter Method

379. This method permits testing large volumes of _______ and is the method of choice
for low-count waters.
A. Colonies D. Heterotrophic organisms
B. Surface water E. MCL
C. Low-turbidity water F. None of the Above
Heterotrophic Plate Count

(Spread Plate Method)
380. Which term uses inorganic carbon sources as their carbon source (food or
substrate)?
A. Colonies D. Heterotrophic organisms
B. Surface growth E. Autotrophic organisms
C. AGAR F. None of the Above

381. Which term provides a technique to quantify the bacteriological activity of a
sample?
A. Colonies D. Heterotrophic Plate Count
B. Heat E. MCL
C. Agar F. None of the Above
382. The R2A agar provides a medium that will support a large variety of?
A. Colonies D. Heterotrophic bacteria
B. Bugs E. MCL
C. Germs F. None of the Above
Total Coliforms
383. According to the text, The MCL is based on the presence of total coliforms, and
compliance is on a daily or weekly basis, depending on your water system type and
state rule.
A. True B. False
384. According to the text, for systems which collect fewer than ______________
samples per month.
A. 5 D. 200
B. 10 E. 40
C. 100 F. None of the Above
385. For systems which collect ___________ or more samples per month, no more than
five (5) percent may be Positive.
A. 5 D. 200
B. 10 E. 40
C. 100 F. None of the Above
Acute Risk to Health (Fecal coliforms and E. coli)

386. A routine analysis shows total coliform present and is followed by a repeat analysis
which indicates?
A. Routine analysis D. Human health violation
B. Drinking violation E. Fecal coliform or E. coli present
C. Water penalty F. None of the Above
387. A routine analysis shows _____________ is followed by a repeat analysis which

indicates total coliform present.
A. Routine analysis D. Presence
B. Drinking water violation E. Total and Fecal coliform or E. coli present
C. MCL violation F. None of the Above
388. Which of the following terms requires the water system to provide public notice via
radio and television stations in the area?
A. Routine analysis violation D. Human health violation
B. Drinking water rule violation E. Acute health risk violation

389. According to the text, the type of contamination can pose an immediate threat to
human health and notice must be given as soon as possible, but no later than 24 hours
after notification from your laboratory of the test results.
A. True B. False
Public Notice
390. A public notice is required to be issued by a water system whenever it fails to
comply with an applicable MCL or?
B. Drinking water rule E. Fecal coliform or E. coli present
C. Treatment technique F. None of the Above
391. This term best describes what also is required whenever a water system fails to
comply with its monitoring and/or reporting requirements or testing procedure.
A. Routine analysis D. Public notice
B. Drinking water rule E. Fecal coliform or E. coli present count
392. There shall be certain information, be issued properly and in a timely manner, and
contain certain_________________ on the public notice.
A. Legal analysis D. Mandatory language
B. Drinking water rule information E. Fecal language
C. NOVs F. None of the Above
393. If there is a(n) __________ present to users, the timing and place of posting of the
public notice may have different priorities.
B. Drinking water rule E. Fecal coliform or E. coli present
C. Acute risk F. None of the Above
The following are acute violations:

394. Which is violation of nitrate?
A. Presence D. Count
B. MCL E. Acute violations
C. MCLG F. None of the Above
395. Concerning total coliforms - when fecal coliforms or E. coli are present in the
distribution system and is a violation of the?
A. Presence D. Count
B. MCL E. Acute violations
C. MCLG F. None of the Above
Waterborne Microorganisms and Bacteria Appendix

Classification
396. Protozoa were commonly grouped in the kingdom of Protista together with the
plant-like algae and fungus-like water molds and slime molds. In the 21st-century
systematics, protozoans, along with ciliates, mastigophorans, and apicomplexans, are
arranged as animal-like protists. However, protozoans are neither Animalia nor Metazoa
(with the possible exception of the enigmatic, moldy Myxozoa).
A. True B. False

397. These pseudopods are also used to capture prey; they simply engulf the food.
They can detect the kind of prey and use different?
A. Eukaryotic cell D. 'Engulfing tactics'
B. Protozoa(ns) E. Cytoplasma
C. Amoeba(s) F. None of the Above
398. Which term means or comprises a unique group of obligate, intracellular parasitic
protozoa?
A. Foraminifera D. Soil biomass
B. Protozoan fauna E. Microsporidia
C. Cytoplasm of protozoa F. None of the Above
399. Many protozoa have_______________, which collect and expel excess water, and
extrusomes, which expel material used to deflect predators or capture prey.
A. Flagella D. Free-living amoebae
B. Contractile vacuoles E. Cell's cytoplasm
C. Vacuole or tonoplast F. None of the Above
400. According to the text, which bug/creature/organism are entirely distinct from
prokaryotic flagella?
A. Eukaryotes D. Free-living amoebae
B. Bacteria or viruses E. Centrioles
C. Protozoa F. None of the Above

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How Diseases Are Transmitted.

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The Main Players- History and Biology Chapter 1

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18. Not all laboratory animals are susceptible to all?

22. Bacteria consist of only?

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Structure of a Eukaryotic Cell

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Structure of a Procaryotic Cell

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What in the World is an Eukaryote?

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