Root System

Currently used in many countries in the world, vetiver grass (Vetiveria zizanioides) applications include soil and water conservation systems in agricultural environment, slope stabilization, mine rehabilitation, contaminated soil and saline land remediation, as well as wastewater treatment. The root system morphology of vetiver was investigated in a small plantation growing on abandoned marl terraces in southern Spain. Root distribution with depth, laterally from the plant, as well as root parameters such as root diameter and tensile strength were also investigated. The profile wall method combined with the block excavation showed that the vetiver grass grows numerous positively gravitropic roots of more or less uniform diameter. These were generally distributed in the uppermost soil horizon closer to the culm base. In situ shear test on blocks of soil permeated with vetiver roots were carried out and showed a greater shear strength resistance than the samples of non vegetated soil. The root reinforcement measured in situ was comparable to the one predicted by the perpendicular root reinforcement model. The stability of a modelled terraced slope planted with vetiver was marginally greater than the one of a nonvegetated slope. A local instability on one terrace can have a detrimental effect on the overall stability of the terraced slope.

In established Australian vineyards compacted soil either created naturally or by trafficking prevents root growth to desirable depths and restricts root systems from getting water and nutrients. This can reduce the life span and productivity of grapevines considerably over a period of years. To break up these compacted soil layers using small tractors a deep working oscillatory ripper was developed at University of South Australia which had two deep working oscillating tines. One of the most important factors for the performance of this oscillatory ripper is the oscillation angle of the tines which is described as the relationship between tine pivot point and tine tip. To find the optimum oscillation angle a series of field tests were conducted in a sand-loam soil at 6 different oscillation angles. The tines oscillated at a frequency of 4.9 Hz and with amplitudes of between ±60-69mm, with a nominal forward speed of 3 km/h. The trial results are quantified based on draft requirement, power saving, power ratio and loosened area. The greatest draft reduction of 50% was achieved for negative oscillation angles, where power ratio was less than one. Power saving was significantly affected by changing oscillation angle. In general, vibration of the driver seat was higher for negative angles. In terms of performance-22.5º oscillation angle was the optimum for draft reduction and power saving, whereas the least vibration was obtained at an oscillation angle of +16º which was equal to Australian Standard threshold for 8 hours continuous work.

Root and soil samples of three potted or ground-grown cycads (Cycas circinalis, C. revoluta, Zamia sp.) were collected between November 1999 and June 2000 and surveyed for arbuscular mycorrhizal (AM) colonization and spore populations. AM fungi were associated with all root systems and rhizosphere samples examined. Root colonization was of a typical Arum type and AM colonization levels differed significantly between species and between potted and groundgrown cycads. Mycorrhizal colonization levels were inversely related to root hair number and length. Spores of nine morphotypes belonging to three genera (Acaulospora, Glomus, Scutellospora) were extracted from soil. The percentage root length colonized by AM fungi was not related to soil factors, but total AM fungal spore numbers in the rhizosphere soil were inversely related to soil nitrogen and phosphorus levels. AM fungal spore numbers in the soil were linearly related to root length colonized. The co-occurrence of septate non-mycorrhizal fungi was recorded for the first time in cycads. These observations and the relationship between plant mycorrhizal status and soil nutrients are discussed.

Extensive and deep root systems have been recognized as one of the most important traits for improving chickpea (Cicer arietinum L.) productivity under progressively receding soil moisture conditions. However, available information on the range of variation for root traits is still limited. Genetic variability for the root traits was investigated using a cylinder culture system during two consecutive growth seasons in the mini-core germplasm collection of ICRISAT plus several wild relatives of chickpea. The largest genetic variability was observed at 35 days after sowing for root length density (RLD) (heritability, h 2 = 0.51 and 0.54) across seasons, and followed by the ratio of plant dry weight to root length density with h 2 of 0.37 and 0.47 for first and second season, respectively. The root growth of chickpea wild relatives was relatively poor compared to C. arietinum, except in case of C. reticulatum. An outstanding genotype, ICC 8261, which had the largest RLD and one of the deepest root system, was identified in chickpea mini-core germplasm collection. The accession ICC 4958 which was previously characterized as a source for drought avoidance in chickpea was confirmed as one with the most prolific and deep root system, although many superior accessions were also identified. The chickpea landraces collected from the Mediterranean and the west Asian region showed a significantly larger RLD than those from the south Asian region. In addition, the landraces originating from central Asia (former Soviet Union), characterized by arid agro-climatic conditions, also showed relatively larger RLD. As these regions are underrepresented in the chickpea collection, they might be interesting areas for further germplasm exploration to identify new landraces with large RLD. The information on the genetic variability of chickpea root traits provides valuable baseline knowledge for further progress on the selection and breeding for drought avoidance root traits in chickpea.

A rootbox is described which allows observation of an intact, entire root system. Roots are sandwiched against a plexiglass surface by a nylon mesh that is impermeable to roots, but permeable to water and nutrients. To quantify root growth non-destructively, roots of different size classes are traced onto acetate sheets using different color pens, and root lengths determined by digital image analysis.

CITATIONS 11 READS 48 4 authors, including: Some of the authors of this publication are also working on these related projects: Zotino Tall Tower Observatory (ZOTTO) View project Russian Science Foundation project RSF#14-14-00219 "Experimental and theoretical analysis of the treering growth variability in mainland of Siberia (the Yenisey-Lena transect)" (2014-2016)) View project Anatoly Prokushkin V.N. Sukachev Institute of forest SB RAS 95 PUBLICATIONS 1,053 CITATIONS SEE PROFILE

Soilless plant growth systems are widely used as a means to save irrigation water and to reduce groundwater contamination. While nutrient concentrations in the growth medium are depleted due to uptake by the plants, salinity and toxic substances accumulate due to transpiration. A theoretical model is suggested, to simulate nutrient uptake by plants grown in soilless cultures with recycled solutions. The model accounts for salinity accumulation with time and plant growth, and its effects on uptake of the different nutrients by means of interaction with Na and Cl ions. The sink term occurs due to uptake by a growing root system. Influx as a function of the ion concentration is according to Michaelis-Menten active mechanisms for K + , NO 3 − -N, NH 4 + -N, PO 4 -P, Ca 2+ , Mg 2+ and SO 4 2− , whose influx parameters are affected by Na and Cl − , but not with time (age). Sodium influx is passive above a critical concentration. Sum of cations-anions concentrations is balanced by Cl − to maintain electro-neutrality of the growth solution. Salinity (by means of Na concentration) suppresses root and leaf growth, which further effect uptake and transpiration. The model accounts for instantaneous transpiration losses, during daytime only and its effect on uptake of nutrients and plant development due to salt accumulation. The model was tested against NO 3 − and K + uptake by plants associated with cumulative transpiration and with different NaCl salinity levels. Deviations from observed K + uptake should be attributed to the salinity tolerance of the plants. In a study with data obtained from published literature, the model indicated that nutrient depletion and salinity buildup might be completely different with fully grown-up plants (that do not grow) and plants that grow with time. Depletion of different nutrients are according to their initial concentration and plant uptake rate, but also affected by their interactions with Na and Cl ions.

Actinorhizal plants form a nodular, nitrogen-fixing root symbiosis with the actinomycete Frankia and are economically and ecologically important due to their ability to improve the nitrogen fertility of disturbed and infertile substrates. In this study, water-retentive polymer inoculum carriers were applied as a root dip. This treatment significantly increased nodulation and in some cases early growth of Alnus glutinosa (L.) Gaertn. and Casuarina equisetifolia var. equisetifolia Forst. & Forst. in a controlled environment and also of A. glutinosa under field conditions. Nodule number and nodule dry weight per plant were at least two to three times greater after 56 to 140 days for plants inoculated with Frankia carried in a water-retentive polymer base compared with plants inoculated with Frankia in water. Nodules on the roots of the plants that were inoculated with Frankia in a polymer slurry were distributed throughout the entire root system, rather than concentrated near the root collar. When amended with water-retentive polymers, actinorhizal plants inoculated with 5-to 10-fold lower titers of Frankia exhibited early growth and nodule numbers equal to or greater than those plants inoculated with standard titers without polymers. The water-retentive, superabsorbent polymers clearly increased the nodulation of two actinorhizal plant species.

Aluminium toxicity as a consequence of increasing soil acidity is discussed as a primary factor in forest decline in middle Europe. Ecologically relevant concentrations of A1 disturbed root systems of hydroponically grown spruce (Picea abies Karst.) seedlings. The needles, with a much lower A1 content in comparison to the roots, were also affected by A1 at these external concentrations. In this investigation needle chlorosis as the visible symptom of A1 toxicity, was accompanied by decreasing pigment concentrations and photosynthetic rates as well as increasing starch levels and transpiration rates. The magnesium concentrations of roots and needles were lowered by treatment with A1. By feeding the needles directly with Mg, it was possible to differentiate between direct and indirect effects of A1 on needle metabolism. Magnesium deficiency was found to drive all the experimental variables except transpiration.

The rhizosphere is a densely populated area in which plant roots must compete with invading root systems of neighboring plants for space, water, and mineral nutrients, and with other soil-borne organisms, including bacteria and fungi. Root-root and root-microbe communications are continuous occurrences in this biologically active soil zone. How do roots manage to simultaneously communicate with neighboring plants, and with symbiotic and pathogenic organisms within this crowded rhizosphere? Increasing evidence suggests that root exudates might initiate and manipulate biological and physical interactions between roots and soil organisms, and thus play an active role in root-root and root-microbe communication. Glossary Allelopathy: Chemical inhibition of one species by another. Allelochemicals: Small molecular weight phyto-inhibitory compounds produced by a plant to ward-off another plant. Chemotaxis: Phenomenon by which a biotic adherence takes place by virtue of the production of a chemical signal. Edaphic factors: Involve both biological and abiotic climatic conditions required to regulate a specific niche. Root exudates: The chemicals secreted into the soil by roots.

Understanding the impact of root architecture on plant resource efficiency is important, in particular, in the light of upcoming shortages of mineral fertilizers and changed environmental conditions. In the 1950s, a great number of root systems of European cultivated plants were excavated and studied by L. Kutschera (1960). Her work gave enormous insight into the variety of root system architectures and helped to realize the importance of belowground processes to plant productivity. We analysed the resulting hand drawings by using mathematical modelling and found root system parameters for a newly developed parametric L-System model. In this way we were able to first reproduce the illustrations, second computationally analyse root system traits and finally access the dynamic root architecture development.

The root growth of trees close to buildings can cause directly or indirectly structural damages and the necessity arises of diagnostic tools to follow their time-spatial behaviour. In this framework, sensing techniques as Ground Penetrating Radar (GPR) and Electrical Resistivity Tomography (ERT) are of relevant interest since they allow in principle the detection and identification of roots in a fast and non invasive way.

Extensive communication occurs between plants and microorganisms during different stages of plant development in which signaling molecules from the two partners play an important role. Volatile organic compounds (VOCs) emission by certain plant-growth promoting rhizobacteria (PGPR) has been found to be involved in plant growth. However, little is known about the role of bacterial VOCs in plant developmental processes. In this work, we investigated the effects of inoculation with twelve bacterial strains isolated from the rhizosphere of lemon plants (Citrus aurantifolia) on growth and development of Arabidopsis thaliana seedlings. Several bacterial strains showed a plant growth promoting effect stimulating biomass production, which was related to differential modulation of root-system architecture. The isolates L263, L266, and L272a stimulated primary root growth and lateral root development, while L254, L265a and L265b did not significantly alter primary root growth but strongly promoted lateral root formation. VOC emission analysis by SPME-GC-MS identified aldehydes, ketones and alcohols as the most abundant compounds common to most rhizobacteria. Other VOCs, including 1-octen-3-ol and butyrolactone were strain specific. Characterization of L254, L266 and L272a bacterial isolates by 16S rDNA analysis revealed the identity of these strains as Bacillus cereus, Bacillus simplex and Bacillus sp, respectively. Taken together, our data suggest that rhizospheric bacterial strains can modulate both plant growth promotion and root-system architecture by differential VOC emission.

Stands of maritime pine (Pinus pinaster Ait.) cover about one million hectares of land in south-western France and produce 19% of all French timber, thanks to the intensive management methods employed. Evaluations of carbon fixation and storage in this forest are facilitated by its general homogeneity with respect to soil, climate and tree genetics. However, initial assessments were based on basic values for expansion factors and carbon concentration in the biomass, and more accurate results could be obtained.

Water for irrigation is limited worldwide. Therefore water saving practices will have to be adopted. This experiment was carried out to compare deficit irrigation (DI) with partial rootzone drying (PRD) for their effects on the processing tomato (Lycopersicon esculentum Mill.) cv. Petopride. The treatments were: full watering of both sides of the root system (RS) at each irrigation as control (C), half of irrigation water in C divided equally to both sides of the RS with each watering (DI), and half of irrigation water in C given only to one side of the RS with each irrigation (PRD). Photosynthetic rate, transpiration, stomatal conductance, and leaf water potential were measured on five occasions, and were found to be the same among treatments. Total fruit fresh mass

High rooting percentages and high-quality adventitious root systems for papaya (Carica papaya L.) were obtained in vitro by appropriate auxin source, duration of exposure to auxin and use of riboflavin. Root initiation of papaya shoots was higher using IBA than IAA, NAA or PCPA. Maximum rooting percentage (96%) was achieved by exposure of shoots to a medium containing 10 I~M IBA for 3 days before transfer to a hormone-free medium. However, the resultant plants had small shoots and callused roots. Shoot and root growth were improved when shoots were transferred after 2 days from medium containing 10 ~M IBA to hormone-free medium containing 10 p.M riboflavin. Good root initiation, and root and shoot growth were also obtained when shoots were incubated for 2 days in darkness on a medium containing 10 IxM IBA and 31 txM riboflavin before transfer to light. Alternatively, cultures could be placed in the light on medium containing 10 I~M IBA, and after 1 day the medium overlaid with 300 ~M riboflavin (1 ml over 10 ml of medium).

Carnivorous plants may benefit from animal-derived nutrients to supplement minerals from the soil. Therefore, the role and importance of their roots is a matter of debate. Aquatic carnivorous species lack roots completely, and many hygrophytic and epiphytic carnivorous species only have a weakly developed root system. In xerophytes, however, large, extended and/or deep-reaching roots and sub-soil shoots develop. Roots develop also in carnivorous plants in other habitats that are hostile, due to flooding, salinity or heavy metal occurance. Information about the structure and functioning of roots of carnivorous plants is limited, but this knowledge is essential for a sound understanding of the plants’ physiology and ecology. Here we compile and summarise available information on: The morphology of the roots. The root functions that are taken over by stems and leaves in species without roots or with poorly developed root systems; anchoring and storage occur by specialized chlorophyll-less stems; water and nutrients are taken up by the trap leaves. The contribution of the roots to the nutrient supply of the plants; this varies considerably amongst the few investigated species. We compare nutrient uptake by the roots with the acquisition of nutrients via the traps. The ability of the roots of some carnivorous species to tolerate stressful conditions in their habitats; e.g., lack of oxygen, saline conditions, heavy metals in the soil, heat during bushfires, drought, and flooding.kg]Key words

Scarcity of water is a severe environmental constraint to plant productivity. Drought-induced loss in crop yield probably exceeds losses from all other causes, since both the severity and duration of the stress are critical. Here, we have reviewed the effects of drought stress on the growth, phenology, water and nutrient relations, photosynthesis, assimilate partitioning, and respiration in plants. This article also describes the mechanism of drought resistance in plants on a morphological, physiological and molecular basis. Various management strategies have been proposed to cope with drought stress. Drought stress reduces leaf size, stem extension and root proliferation, disturbs plant water relations and reduces water-use efficiency. Plants display a variety of physiological and biochemical responses at cellular and whole-organism levels towards prevailing drought stress, thus making it a complex phenomenon. CO2 assimilation by leaves is reduced mainly by stomatal closure, membrane damage and disturbed activity of various enzymes, especially those of CO2 fixation and adenosine triphosphate synthesis. Enhanced metabolite flux through the photorespiratory pathway increases the oxidative load on the tissues as both processes generate reactive oxygen species. Injury caused by reactive oxygen species to biological macromolecules under drought stress is among the major deterrents to growth. Plants display a range of mechanisms to withstand drought stress. The major mechanisms include curtailed water loss by increased diffusive resistance, enhanced water uptake with prolific and deep root systems and its efficient use, and smaller and succulent leaves to reduce the transpirational loss. Among the nutrients, potassium ions help in osmotic adjustment; silicon increases root endodermal silicification and improves the cell water balance. Low-molecular-weight osmolytes, including glycinebetaine, proline and other amino acids, organic acids, and polyols, are crucial to sustain cellular functions under drought. Plant growth substances such as salicylic acid, auxins, gibberrellins, cytokinin and abscisic acid modulate the plant responses towards drought. Polyamines, citrulline and several enzymes act as antioxidants and reduce the adverse effects of water deficit. At molecular levels several drought-responsive genes and transcription factors have been identified, such as the dehydration-responsive element-binding gene, aquaporin, late embryogenesis abundant proteins and dehydrins. Plant drought tolerance can be managed by adopting strategies such as mass screening and breeding, marker-assisted selection and exogenous application of hormones and osmoprotectants to seed or growing plants, as well as engineering for drought resistance.

A rapid and accurate assessment of root morphological characteristics can be important for identifying promising plant genotypes. Scanner-based image analysis has made the measurement of root morphology faster, more accurate, and less subjective, and allows for estimation of fractal dimension (FD). We used scanner-based image analysis to investigate root morphology in leafy vs. conventional maize (Zea mays) genotypes. Two experiments were conducted following a completely randomized design with ten and five replicates. In the first experiment, treatments were factorial combinations of four hybrids (leafy reduced-stature (LRS), non leafy-reduced stature (NLRS), leafy normal stature (LNS), and non leafy normal stature (conventional commercial) hybrid, Pioneer 3979 (P3979)), and four sampling dates (5, 10, 15, and 20 days from transfer to growth pouches). In the second experiment, plants were grown for 15 days from emergence in 10-L plastic pots. This experiment included three maize gen...

Scarcity of water is a severe environmental constraint to plant productivity. Drought-induced loss in crop yield probably exceeds losses from all other causes, since both the severity and duration of the stress are critical. Here, we have reviewed the effects of drought stress on the growth, phenology, water and nutrient relations, photosynthesis, assimilate partitioning, and respiration in plants. This article also describes the mechanism of drought resistance in plants on a morphological, physiological and molecular basis. Various management strategies have been proposed to cope with drought stress. Drought stress reduces leaf size, stem extension and root proliferation, disturbs plant water relations and reduces water-use efficiency. Plants display a variety of physiological and biochemical responses at cellular and whole-organism levels towards prevailing drought stress, thus making it a complex phenomenon. CO2 assimilation by leaves is reduced mainly by stomatal closure, membrane damage and disturbed activity of various enzymes, especially those of CO2 fixation and adenosine triphosphate synthesis. Enhanced metabolite flux through the photorespiratory pathway increases the oxidative load on the tissues as both processes generate reactive oxygen species. Injury caused by reactive oxygen species to biological macromolecules under drought stress is among the major deterrents to growth. Plants display a range of mechanisms to withstand drought stress. The major mechanisms include curtailed water loss by increased diffusive resistance, enhanced water uptake with prolific and deep root systems and its efficient use, and smaller and succulent leaves to reduce the transpirational loss. Among the nutrients, potassium ions help in osmotic adjustment; silicon increases root endodermal silicification and improves the cell water balance. Low-molecular-weight osmolytes, including glycinebetaine, proline and other amino acids, organic acids, and polyols, are crucial to sustain cellular functions under drought. Plant growth substances such as salicylic acid, auxins, gibberrellins, cytokinin and abscisic acid modulate the plant responses towards drought. Polyamines, citrulline and several enzymes act as antioxidants and reduce the adverse effects of water deficit. At molecular levels several drought-responsive genes and transcription factors have been identified, such as the dehydration-responsive element-binding gene, aquaporin, late embryogenesis abundant proteins and dehydrins. Plant drought tolerance can be managed by adopting strategies such as mass screening and breeding, marker-assisted selection and exogenous application of hormones and osmoprotectants to seed or growing plants, as well as engineering for drought resistance.

A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1–1.0 mg/l), α-naphthalene acetic acid (0.01–0.2 mg/l), thidiazuron (0.5–2.0 mg/l), 6-benzylaminopurine (0.5–1.0 mg/l), and kinetin (0.5–1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l α-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto–Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l α-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto–Teixeira-3. Shoots formed a strong adventitious root system on New Winarto–Teixeira-3 containing 0.2 mg/l α-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14–18), 15 aneuploid (n = 20–26), 126 diploid (n = 28–34) and 5 triploid (n = 45–57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed.

Protozoan communities around roots with different types of ectomycorrhizae were distinct. These protozoan communities differed both qualitatively and quantitatively with the host (Pinus ponderosa, Pseudotsuga menziesii, Picea sitchensis, Tsuga heterophylla and Abies grandis) and the ectomycorrhizal fungal species.

Soil physical properties play an important role in vegetation growth on rangelands as a result of the effects they have on the development of root systems. The goal of this research was to investigate the trampling effect of livestock grazing on soil physical properties (moisture, bulk density, infiltration rate and mechanical resistance) and vegetation cover. The experiment was conducted on three rangeland condition areas - the reference area, a moderately grazed area and a heavily grazed area - in the Lar rangelands (84 km northeast of Tehran) during the grazing season of 2000 and 2001. Soil moisture was reduced from both the reference area and the heavily grazed area throughout the grazing season to depths of 0-15 and 15-30 cm. Soil bulk density increased during the grazing season, being lowest in the reference area and highest in the heavily grazed area. Throughout the grazing season, bulk density was higher in the top soil layer (0-15 cm) compared to underlying layer (15-30 cm)...

The potential of Leucaena leucocephala as an erosion control plant was investigated in terms of its capacity of root reinforcement, root profile and root shear strength. The species studied exhibited extensive, dense rooting and depth of penetration. These aspects, to some extent, could provide surficial as well as deep-seated erosion control. A high water absorption capacity would increase suction, thus potentially extracting water at the greater depth. The soil-root matrix significantly affects cohesion factor but not the angle of friction. The effect varies with increasing depth and age of plant depending on the root length density. After 6 months of growth, the cohesion factor had tremendously increased by two-to fivefold (0.1-0.5 m soil depth). This range almost reached the values of those in the 12-month treatment, indicating a high contribution of the root system to soil-root reinforcement.

1 In water-limited environments, the availability of water and nutrients to plants depends on environmental conditions, sizes and shapes of their root systems, and root competition. The goal of this study was to predict root system sizes and shapes for different plant growth forms using data on above-ground plant sizes, climate and soil texture. 2 A new data set of > 1300 records of root system sizes for individual plants was collected from the literature for deserts, scrublands, grasslands and savannas with ≤ 1000 mm mean annual precipitation (MAP). Maximum rooting depths, maximum lateral root spreads and their ratios were measured.

A major challenge for mineral explorers is to efficiently detect mineralisation beneath the weathered cover that extends across the landscape. Determination of the elemental composition of plants (biogeochemical exploration) can aid in the detection of buried ore deposits due to their root penetration through the weathered cover. At the coyote prospect in Western Australia a range of plant species were sampled traversing a buried Au orebody. Here it is shown that the soft spinifex (Triodia pungens) accumulated important pathfinder elements related to the mineralisation, which produced a multi-element surficial expression of the underlying ore deposit. Spinifex grasses are one of the most widespread grasses over the Australian continent and have vertical root systems that can extend for many 10s of metres through sedimentary cover and interact with buried mineral deposits and alteration zones in the underlying substrate. Spinifex biogeochemistry therefore has potential to be a low cost, low environmental impact, sampling medium for mineral exploration programs across large parts of arid Australia. The examination of similar grasses from other semi-arid and arid regions from around the world therefore also warrants further investigation.

Coal-based power generation is a principal source of electricity in India and many other countries. About 15-30% of the total amount of residue generated during coal combustion is fly ash (FA). FA is generally alkaline in nature and contains many toxic metals like Cr, Pb, Hg, As and Cd along with many essential elements like S, B, Ca, Na, Fe, Zn, Mn and P. Dumped FA contaminates the biosphere by mobilization of its fine particles and hazardous metals. Despite the negative environmental impact of FA, coal continues to be a major source of power production in India and therefore FA disposal is a major environmental issue. To overcome this problem, FA dumping sites have been started as a potential resource for biomass production of tree species. Phytoremediation is a strategy that uses plants to degrade, stabilize, and remove contaminants from soils, water and waste FA. Phytomanagement of FA is based on the plants' root systems, high biomass, woody nature, native nature, and resistance to pH, salinity, and toxic metals. Recently Indian researchers mostly from the National Botanical Research Institute have been working on phytoremediation and revegetation of FA dykes, inoculation of bacterial strains for reducing FA stress and biomass production from FA dykes. Many international researchers have worked on reclamation, revegetation and utilization of FA. FA utilization saves resources, mainly land (topsoil), water, coal, limestone and chemical fertilizer. Safe utilization of FA is a major concern around the world and regulatory bodies are enforcing stringent rules for the proper management of FA. This article summarizes various viable avenues in India for FA utilization and environmental management.

In present-day alluvial environments, the impact of vegetation on sedimentological processes and deposits is well known. A vegetated catchment may decrease sediment yield, sediment erodibility, Hortonian overland flow, aeolian winnowing of fines, the proportion of sediment transported as bedload, and may increase bank stability, infiltration into substrates, and bed roughness. Vegetation also promotes the production of chemically-weathered clays and soils and the adoption of a meandering style. It is generally understood that, prior to the evolution of terrestrial vegetation during the Early Palaeozoic, ancient alluvial systems were markedly different from modern systems, with many systems adopting a "sheet-braided" style. This understanding has previously informed the interpretations of many Precambrian pre-vegetation alluvial successions, but there has been relatively little work regarding Early Palaeozoic alluvial successions laid down prior to and during the initial colonization of the Earth's surface by plants.

Scarcity of water is a severe environmental constraint to plant productivity. Drought-induced loss in crop yield probably exceeds losses from all other causes, since both the severity and duration of the stress are critical. Here, we have reviewed the effects of drought stress on the growth, phenology, water and nutrient relations, photosynthesis, assimilate partitioning, and respiration in plants. This article also describes the mechanism of drought resistance in plants on a morphological, physiological and molecular basis. Various management strategies have been proposed to cope with drought stress. Drought stress reduces leaf size, stem extension and root proliferation, disturbs plant water relations and reduces water-use efficiency. Plants display a variety of physiological and biochemical responses at cellular and whole-organism levels towards prevailing drought stress, thus making it a complex phenomenon. CO2 assimilation by leaves is reduced mainly by stomatal closure, membrane damage and disturbed activity of various enzymes, especially those of CO2 fixation and adenosine triphosphate synthesis. Enhanced metabolite flux through the photorespiratory pathway increases the oxidative load on the tissues as both processes generate reactive oxygen species. Injury caused by reactive oxygen species to biological macromolecules under drought stress is among the major deterrents to growth. Plants display a range of mechanisms to withstand drought stress. The major mechanisms include curtailed water loss by increased diffusive resistance, enhanced water uptake with prolific and deep root systems and its efficient use, and smaller and succulent leaves to reduce the transpirational loss. Among the nutrients, potassium ions help in osmotic adjustment; silicon increases root endodermal silicification and improves the cell water balance. Low-molecular-weight osmolytes, including glycinebetaine, proline and other amino acids, organic acids, and polyols, are crucial to sustain cellular functions under drought. Plant growth substances such as salicylic acid, auxins, gibberrellins, cytokinin and abscisic acid modulate the plant responses towards drought. Polyamines, citrulline and several enzymes act as antioxidants and reduce the adverse effects of water deficit. At molecular levels several drought-responsive genes and transcription factors have been identified, such as the dehydration-responsive element-binding gene, aquaporin, late embryogenesis abundant proteins and dehydrins. Plant drought tolerance can be managed by adopting strategies such as mass screening and breeding, marker-assisted selection and exogenous application of hormones and osmoprotectants to seed or growing plants, as well as engineering for drought resistance.

Management of avocado trees for optimal yields requires the maintenance of a healthy root system that is fully functional for nutrient and water uptake and growth of beneficial microorganisms that help to suppress root disease. Many different physical, chemical and biological factors influence root growth, all of which interact to influence conditions for development of the root system. This review provides an overview of best management techniques that growers should consider and examines the effects of individual soil factors on root function. The benefits of mulching, proper fertilizer timing, ir - rigation and salinity management are considered in detail as variables that can be managed to optimize avocado yields and root health.

To any finite group Γ⊂Sp(V) of automorphisms of a symplectic vector space V we associate a new multi-parameter deformation, Hκ of the algebra ℂ[V]#Γ, smash product of Γ with the polynomial algebra on V. The parameter κ runs over points of ℙ r , where r=number of conjugacy classes of symplectic reflections in $Γ$. The algebra Hκ, called a symplectic reflection algebra, is related to the coordinate ring of a Poisson deformation of the quotient singularity V/Γ. This leads to a symplectic analogue of McKay correspondence, which is most complete in case of wreath-products. If Γ is the Weyl group of a root system in a vector space ? and V=?⊕?*, then the algebras Hκ are certain ‘rational’ degenerations of the double affine Hecke algebra introduced earlier by Cherednik. Let $Γ=S n , the Weyl group of ?=?? n . We construct a 1-parameter deformation of the Harish-Chandra homomorphism from ?(?)?, the algebra of invariant polynomial differential operators on ?? n , to the algebra of S n -invariant differential operators with rational coefficients on the space ℂ n of diagonal matrices. The second order Laplacian on ? goes, under the deformed homomorphism, to the Calogero-Moser differential operator on ℂ n , with rational potential. Our crucial idea is to reinterpret the deformed Harish-Chandra homomorphism as a homomorphism: ?(?)? ↠ spherical subalgebra in Hκ, where Hκ is the symplectic reflection algebra associated to the group Γ=S n . This way, the deformed Harish-Chandra homomorphism becomes nothing but a description of the spherical subalgebra in terms of ‘quantum’ Hamiltonian reduction. In the ‘classical’ limit κ→∞, our construction gives an isomorphism between the spherical subalgebra in H∞ and the coordinate ring of the Calogero-Moser space. We prove that all simple H∞-modules have dimension n!, and are parametrised by points of the Calogero-Moser space. The family of these modules forms a distinguished vector bundle on the Calogero-Moser space, whose fibers carry the regular representation of S n . Moreover, we prove that the algebra $H∞ is isomorphic to the endomorphism algebra of that vector bundle.

The rhizosphere is the soil-plant root interphase and in practice consists of the soil adhering to the root besides the loose soil surrounding it. Plant growth-promoting rhizobacteria (PGPR) are potential agents for the biological control of plant pathogens. A biocontrol strain should be able to protect the host plant from pathogens and fulfill the requirement for strong colonization. Numerous compounds that are toxic to pathogens, such as HCN, phenazines, pyrrolnitrin, and pyoluteorin as well as, other enzymes, antibiotics, metabolites and phytohormones are the means by which PGPR act, just as quorum sensing and chemotaxis which are vital for rhizosphere competence and colonization. The presence of root exudates has a pronounced effect on the rhizosphere where they serve as an energy source, promoting growth and influencing the root system for the rhizobacteria. In certain instances they have products that inhibit the growth of soil-borne pathogens to the advantage of the plant root. A major source of concern is reproducibility in the field due to the complex interaction between the plant (plant species), microbe and the environment (soil fertility and moisture, day length, light intensity, length of growing season, and temperature). This review listed most of the documented PGPR genera and discussed their exploitation.

A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1-1.0 mg/l), a-naphthalene acetic acid (0.01-0.2 mg/l), thidiazuron (0.5-2.0 mg/l), 6-benzylaminopurine (0.5-1.0 mg/l), and kinetin (0.5-1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto-Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto-Teixeira-3. Shoots formed a strong adventitious root system on New Winarto-Teixeira-3 containing 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14-18), 15 aneuploid (n = 20-26), 126 diploid (n = 28-34) and 5 triploid (n = 45-57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed.

† Background Salt stress has become a major threat to plant growth and productivity. Arbuscular mycorrhizal fungi colonize plant root systems and modulate plant growth in various ways. † Scope This review addresses the significance of arbuscular mycorrhiza in alleviation of salt stress and their beneficial effects on plant growth and productivity. It also focuses on recent progress in unravelling biochemical, physiological and molecular mechanisms in mycorrhizal plants to alleviate salt stress. † Conclusions The role of arbuscular mycorrhizal fungi in alleviating salt stress is well documented. This paper reviews the mechanisms arbuscular mycorrhizal fungi employ to enhance the salt tolerance of host plants such as enhanced nutrient acquisition (P, N, Mg and Ca), maintenance of the K þ : Na þ ratio, biochemical changes (accumulation of proline, betaines, polyamines, carbohydrates and antioxidants), physiological changes ( photosynthetic efficiency, relative permeability, water status, abscissic acid accumulation, nodulation and nitrogen fixation), molecular changes (the expression of genes: PIP, Na þ /H þ antiporters, Lsnced, Lslea and LsP5CS) and ultra-structural changes. Theis review identifies certain lesser explored areas such as molecular and ultrastructural changes where further research is needed for better understanding of symbiosis with reference to salt stress for optimum usage of this technology in the field on a large scale. This review paper gives useful benchmark information for the development and prioritization of future research programmes.

Scarcity of water is a severe environmental constraint to plant productivity. Drought-induced loss in crop yield probably exceeds losses from all other causes, since both the severity and duration of the stress are critical. Here, we have reviewed the effects of drought stress on the growth, phenology, water and nutrient relations, photosynthesis, assimilate partitioning, and respiration in plants. This article also describes the mechanism of drought resistance in plants on a morphological, physiological and molecular basis. Various management strategies have been proposed to cope with drought stress. Drought stress reduces leaf size, stem extension and root proliferation, disturbs plant water relations and reduces water-use efficiency. Plants display a variety of physiological and biochemical responses at cellular and whole-organism levels towards prevailing drought stress, thus making it a complex phenomenon. CO2 assimilation by leaves is reduced mainly by stomatal closure, membrane damage and disturbed activity of various enzymes, especially those of CO2 fixation and adenosine triphosphate synthesis. Enhanced metabolite flux through the photorespiratory pathway increases the oxidative load on the tissues as both processes generate reactive oxygen species. Injury caused by reactive oxygen species to biological macromolecules under drought stress is among the major deterrents to growth. Plants display a range of mechanisms to withstand drought stress. The major mechanisms include curtailed water loss by increased diffusive resistance, enhanced water uptake with prolific and deep root systems and its efficient use, and smaller and succulent leaves to reduce the transpirational loss. Among the nutrients, potassium ions help in osmotic adjustment; silicon increases root endodermal silicification and improves the cell water balance. Low-molecular-weight osmolytes, including glycinebetaine, proline and other amino acids, organic acids, and polyols, are crucial to sustain cellular functions under drought. Plant growth substances such as salicylic acid, auxins, gibberrellins, cytokinin and abscisic acid modulate the plant responses towards drought. Polyamines, citrulline and several enzymes act as antioxidants and reduce the adverse effects of water deficit. At molecular levels several drought-responsive genes and transcription factors have been identified, such as the dehydration-responsive element-binding gene, aquaporin, late embryogenesis abundant proteins and dehydrins. Plant drought tolerance can be managed by adopting strategies such as mass screening and breeding, marker-assisted selection and exogenous application of hormones and osmoprotectants to seed or growing plants, as well as engineering for drought resistance.

The contribution of plant root systems to slope stability and soil erosion control has received a lot of attention in recent years. The plant root system is an intricate and adaptive object, and understanding the details of soil-root interaction is a difficult task. Although the morphology of a root system greatly influences its soilfixing efficiency, limited architectural work has been done in the context of slope stabilization and erosion control, and hence it remains unknown exactly which characteristics are important. Many of the published research methods are tedious and time-consuming. This review deals with the underlying mechanisms of shallow slope stabilization and erosion control by roots, especially as determined by their architectural characteristics. The effect of soil properties as well as the relative importance of different root sizes and of woody versus non-woody species are briefly discussed.

This review emphasizes recent developments and controversies related to the uptake, transport and loss of water by trees. Comparisons of the stable isotope composition of soil and xylem water have provided new and sometimes unexpected insights concerning spatial and temporal partitioning of soil water by roots. Passive, hydraulic redistribution of water from moister to drier portions of the soil profile via plant root systems may have a substantial impact on vertical profiles of soil water distribution, partitioning of water within and among species, and on ecosystem water balance. The recent development of a technique for direct measurement of pressure in individual xylem elements of intact, transpiring plants elicited a number of challenges to the century-old cohesion-tension theory. The ongoing debate over mechanisms of long-distance water transport has stimulated an intense interest in the phenomenon and mechanisms of embolism repair. Rather than embolism being essentially irreversible, it now appears that there is a dynamic balance between embolism formation and repair throughout the day and that daily release of water from the xylem via cavitation may serve to stabilize leaf water balance by minimizing the temporal imbalance between water supply and demand. Leaf physiology is closely linked to hydraulic architecture and hydraulic perturbations, but the precise nature of the signals to which stomata respond remains to be elucidated. When water transport in trees is studied at multiple scales from single leaves to the whole organism, considerable functional convergence in regulation of water use among phylogenetically diverse species is revealed.

The availability of nitrogen in sediment phytodetritus to seagrass was investigated in 5 tropical species (Thalassia hemprichii, Halodule uninervis, H, pinifolia, Halophila ovalis/ovata and Syringodium isoetifolium) from Indonesia. N-15-labeled phytodetritus was injected into the sediment and the appearance of N-15 in the roots, the rhizomes and the leaves of the plants were measured after 1, 2, 4 and 8 d. The transfer of N-15 from sediment phytodetritus to the plant tissues was very rapid (within 1 d) and continued over the 8 d period, indicating an efficient retention of the phytodetritus and a net mineralization of nitrogen rather than an immobilisation by bacteria. Relative enrichment of roots versus leaves indicated that N-15 was taken up by the roots and then transferred to the leaves. The combined efficiency of seagrass canopy-induced trapping and retention of sestonic particles and root-uptake, results in the acquisition of nutrients released upon mineralization of particula...

Plant roots serve a multitude of functions in the plant including anchorage, provision of nutrients and water, and production of exudates with growth regulatory properties. The root-soil interface, or rhizosphere, is the site of greatest activity within the soil matrix. Within this matrix, roots affect soil structure, aeration and biological activity as they are the major source of organic inputs into the rhizosphere, and are also responsible for depletion of large supplies of inorganic compounds. Roots are very complicated morphologically and physiologically, and their metabolites are often released in large quantities into the soil rhizosphere from living root hairs or fibrous root systems. Root exudates containing root-specific metabolites have critical ecological impacts on soil macro and microbiota as well as on the whole plant itself. Through the exudation of a wide variety of compounds, roots impact the soil microbial community in their immediate vicinity, influence resistance to pests, support beneficial symbioses, alter the chemical and physical properties of the soil, and inhibit the growth of competing plant species. In this review, we outline recent research on root exudation and the role of allelochemicals in the rhizosphere by studying the case of three plants that have been shown to produce allelopathic root exudates: black walnut, wheat and sorghum