Lab Experiment 2

Republic of the Philippines
BATANGAS STATE UNIVERSITY

The National Engineering University
Alangilan Campus
Golden Country Homes, Alangilan Batangas City, Batangas, Philippines 4200
Tel Nos.: (+63 43) 425-0139 local 2121 / 2221
E-mail Address: [email protected] | Website Address: http://www.batstate-u.edu.ph
General Education Office
College of Engineering
Department of Electrical Engineering

Bachelor of Science in Computer Engineering
SCI 402
MODERN BIOLOGY
Laboratory Experiment # 2
MICROSCOPIC OBSERVATION OF MICROORGANISMS
Submitted by:
GROUP MEMBERS (IN ALPHABETICAL ORDER)
SECTION
Submitted to:
INSTRUCTOR
DATE
Leading Innovations, Transforming Lives, Building the Nation

Alangilan Campus
Tel Nos.: (+63 43) 425-0139 local 2121 / 2221
Group No: Date Performed:

Section: Date Submitted:
Lab Activity No. 2

Microscopic Observation of Microorganisms
I. Introduction
In this laboratory activity, we will explore the microscopic world of
microorganisms, including bacteria, fungi, and protozoa. These organisms are crucial
components of various ecosystems and play significant roles in environmental
processes and human health. Using an electron microscope, we will observe and
identify these microorganisms, gaining insights into their structures, behaviors, and
ecological importance. Through this hands-on exploration, we aim to gain a deeper
understanding of the world of microorganisms and the significance of microscopic
observation in microbiology.
II. Objectives:
•To observe and identify microorganisms (bacteria, fungi, and protozoa) using a
electron microscope.
•To understand the roles of microorganisms in different habitats and their impact on
ecosystems.
III.Materials:
- Electron microscope
- Microscope slides
- Cover slips
- Dropper or pipette
- Distilled water
- Cotton swabs
-Sample sources (pond water, yogurt/Yakult, slice of bread with visible molds)
- Microscope slide labels
- Permanent marker
IV. Procedures:
1. Preparation of Microscope Slides:
a. Place a clean microscope slide on a flat, clean surface.
b. Using a dropper or pipette, place a small drop of distilled water in the center of
the slide.
c. If working with solid samples:
For yogurt (bacteria): Gently streak a small portion of yogurt onto the drop of
water using a sterile cotton swab.
For bread (fungi): Press a small section of moldy bread onto the slide.
d. For liquid samples (e.g., pond water for protozoa), use a dropper to place a small
sample onto the drop of water on the slide.
e. Place a cover slip over the drop of water, gently lowering it at an angle to avoid air
bubbles. Ensure the cover slip rests evenly on the sample.

Alangilan Campus
Tel Nos.: (+63 43) 425-0139 local 2121 / 2221
2. Labeling the Slides:

a. Use microscope slide labels and a permanent marker to label each slide with the
source of the sample and the date.
3. Microscopic Observation:
a. Place the prepared slide on the stage of the electron microscope.
b. Begin with the lowest magnification setting to locate and focus on the
microorganisms.
c. Adjust the focus using the appropriate knobs until the sample comes into clear
view.
d. Increase the magnification to observe microorganisms more closely.
e. Take note of the size, shape, and arrangement of any microorganisms you
observe.
4. Recording Observations:
a. Record your observations in a notebook, noting the morphology, arrangement, and

any other notable features of the observed microorganisms.
V. Data and Observations

(Compile all recorded observations into a comprehensive data set for analysis and
discussion in yoghurt, bread, and pond water)

Alangilan Campus
Tel Nos.: (+63 43) 425-0139 local 2121 / 2221
VI. Guide Questions:

Yoghurt
1. What did you observe in the yogurt sample under the microscope? Were you able to
identify any rod-shaped bacteria that might be probiotic bacteria?
2. Describe the potential benefits of the microorganisms found in yogurt for human
health.
Slice of bread with Molds:

3. What did you observe in the mold on the bread sample under the microscope?
Describe the structures and characteristics of fungal cells.
4. How do fungi contribute to the decomposition process of organic matter like bread?
Pond Water
5. What microorganisms did you observe in the pond water sample under the
microscope? Describe their shapes, sizes, and movements.
6. Discuss the ecological significance of microorganisms in pond ecosystems.
VII. Conclusion:

Lab Experiment 2

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Republic of the Philippines

BATANGAS STATE UNIVERSITY

General Education Office

Department of Electrical Engineering

MICROSCOPIC OBSERVATION OF MICROORGANISMS

Leading Innovations, Transforming Lives, Building the Nation

General Education Office

Group No: Date Performed:

Lab Activity No. 2

Leading Innovations, Transforming Lives, Building the Nation

General Education Office

2. Labeling the Slides:

a. Record your observations in a notebook, noting the morphology, arrangement, and

V. Data and Observations

Leading Innovations, Transforming Lives, Building the Nation

General Education Office

VI. Guide Questions:

Slice of bread with Molds:

6. Discuss the ecological significance of microorganisms in pond ecosystems.

Leading Innovations, Transforming Lives, Building the Nation

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