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    Cardio Practical 2&3

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    mk5152313
    Jfh

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    © All Rights Reserved
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    Cardio Practical 2&3

    Uploaded by

    mk5152313
    2 views4 pages
    Jfh

    Original Title

    Cardio practical 2&3

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    © © All Rights Reserved

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    Jfh

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    2 views4 pages

    Cardio Practical 2&3

    Uploaded by

    mk5152313
    Jfh

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    Practical Examining Blood Smears for Plasmodium Parasites

    Materials Needed:
     Microscope with oil immersion lens
     Glass slides and cover slips
     Sterile lancet or needle
     Alcohol swabs
     Blood sample
     Wright-Giemsa stain
     Staining tray
     Immersion oil
     Gloves
     Disinfectant
    Preparation of Blood Smear:
    1. Collect Blood:
     Clean fingertip with alcohol swab.
     Prick finger with sterile lancet.
     Place a drop of blood near one end of a slide.
    2. Prepare Smear:
     Use another slide to spread the blood drop thinly across the slide.
     Label the slide.
    Staining the Blood Smear:
    3. Air Dry:
     Let the smear air dry completely.
    4. Fixing (if required):
     Cover the dried smear with methanol for 1-2 minutes, then air dry.
    5. Staining:
     Cover smear with Wright-Giemsa stain for 10-15 minutes.
     Rinse gently with water and air dry.
    Examining the Blood Smear:
    6. Microscope Setup:
     Turn on the light and place the slide on the stage.
     Start with a lower magnification (10x or 40x).
    7. Oil Immersion:
     Apply immersion oil on the stained smear.
     Rotate the 100x objective lens into place.
    8. Examine Smear:
     Adjust the fine focus.
     Systematically scan the smear.
     Look for Plasmodium parasites (ring-shaped trophozoites, schizonts, and
    gametocytes).
    Recording and Reporting:
    9. Document Findings:
     Record the presence and stage of any parasites.
     Estimate parasitemia if required.
    10. Clean Up:
     Dispose of lancets and gloves properly.
     Clean the microscope and work area.
    Practical Examining Stool Samples for Roundworm (Ascaris) Eggs
    Materials Needed:
     Microscope
     Glass slides and cover slips
     Stool sample
     Applicator stick or spatula
     Saline solution or iodine stain
     Gloves
     Disinfectant
    Preparation of Stool Sample:
    1. Collect Sample:
     Use an applicator stick to obtain a small amount of stool.
     Place a small portion of stool on a glass slide.
    2. Prepare Smear:
     Mix the stool sample with a drop of saline solution or iodine stain.
     Spread the mixture thinly on the slide.
     Place a cover slip over the smear.
    Examining the Stool Smear:
    3. Microscope Setup:
     Turn on the light and place the slide on the microscope stage.
     Start with a lower magnification (10x or 40x).
    4. Examine Smear:
     Adjust the fine focus.
     Systematically scan the smear.
     Look for characteristic Ascaris eggs, which are oval with a thick shell and sometimes
    a mammillated outer layer.
    Recording and Reporting:
    5. Document Findings:
     Record the presence of Ascaris eggs.
     Estimate the egg count if required for quantification.
    6. Clean Up:
     Dispose of gloves and applicator sticks properly.
     Clean the microscope and work area with disinfectant.

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