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    1-Microscopy Lab Instruction Summary

    Uploaded by

    Anastasia Veldboom
    The document provides instructions for microscopy procedures including arrival, simple staining, hanging drop preparation, and clean up. Arrival procedures include putting on PPE like a face mask, lab coat, and gloves. Simple staining involves making a smear, heat fixing, staining with methylene blue, and observing under a microscope. Hanging drop preparation involves placing a sample between a slide and coverslip with Vaseline to create a hanging drop for observation. Clean up involves disposing of materials properly and disinfecting work areas and equipment.

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    © All Rights Reserved
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    1-Microscopy Lab Instruction Summary

    Uploaded by

    Anastasia Veldboom
    18 views4 pages
    The document provides instructions for microscopy procedures including arrival, simple staining, hanging drop preparation, and clean up. Arrival procedures include putting on PPE like a face mask, lab coat, and gloves. Simple staining involves making a smear, heat fixing, staining with methylene blue, and observing under a microscope. Hanging drop preparation involves placing a sample between a slide and coverslip with Vaseline to create a hanging drop for observation. Clean up involves disposing of materials properly and disinfecting work areas and equipment.

    Original Description:

    Microbiology Lab 1 Front Screen - Arrival Procedures, Simple Stain, Hanging Drop

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    The document provides instructions for microscopy procedures including arrival, simple staining, hanging drop preparation, and clean up. Arrival procedures include putting on PPE like a face mask, lab coat, and gloves. Simple staining involves making a smear, heat fixing, staining with methylene blue, and observing under a microscope. Hanging drop preparation involves placing a sample between a slide and coverslip with Vaseline to create a hanging drop for observation. Clean up involves disposing of materials properly and disinfecting work areas and equipment.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    18 views4 pages

    1-Microscopy Lab Instruction Summary

    Uploaded by

    Anastasia Veldboom
    The document provides instructions for microscopy procedures including arrival, simple staining, hanging drop preparation, and clean up. Arrival procedures include putting on PPE like a face mask, lab coat, and gloves. Simple staining involves making a smear, heat fixing, staining with methylene blue, and observing under a microscope. Hanging drop preparation involves placing a sample between a slide and coverslip with Vaseline to create a hanging drop for observation. Clean up involves disposing of materials properly and disinfecting work areas and equipment.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    Microscopy

    ARRIVAL PROCEDURES

     Put on Face Mask


     Find Work Station
     Place personal belongings in cabinet near
    Work Station
     Roll Up Sleeves and Tie Back Hair, if necessary
     Wash Hands
     Put on Lab Coat
    o Roll up sleeves, if long

     Put on Goggles
    o Select pair from white cabinet near autoclave room

     Put on Gloves
    o Be careful not to touch your face, hair, face mask, or goggles with
    gloves on
    o Change gloves whenever they become contaminated

     Disinfect Work Station


    Microscopy

    Simple Stain

    Prepare a Smear:
    1) Collect sample with clean wooden stick
    and apply to slide, rough side up
    2) Dispose of swab/stick in small biohazard
    container on table
    3) Air-dry slide COMPLETELY (may use
    slide warmer for this step)
    4) Heat-fix, using clothespin

    Simple Stain:
    1) Cover smear with methylene blue for 1
    minute
    2) Rinse stain off gently with wash bottle
    3) Remove clothespin, and pat slide dry
    with Bibulous Paper
    4) Observe with microscope
    Microscopy
    HANGING-DROP
    1) Place slide and coverslip separately on top of “Kimwipe” tissue
    2) Put a dot of Vaseline on the 4 corners of the cover slip
    3) Collect 2 loops of Proteus mirabilis and place on a cover slip:
    a) Flame loop
    b) Open tube, flame lip
    c) Collect first sample
    d) Flame lip, close tube
    e) Apply sample to cover slip, repeat steps a)-e) at least one
    more time
    4) Carefully attach a depression slide to the cover slip
    5) Turn, so cover slip is on top
    6) Observe on HIGH POWER, before proceeding to Oil Immersion
    7) When you are finished, place slide and cover slip both in a
    designated Beaker of Disinfectant on Hanging-Drop Cart,
    DO NOT Discard Depression Slides into Sharps Container!!!

    IF YOU CANNOT SEE BACTERIA MOVING, DO NOT MAKE A FRESH SLIDE UNTIL YOU TRY
    THE TRICKS BELOW:
    1) Use low light: Keep your lamp BRIGHT, but close the IRIS DIAPHRAGM
    2) Locate the edge of your drop on SCAN. The drop will appear grainy. The graininess
    is the bacteria
    3) As you increase magnification, keep the edge of the drop in view and increase light
    as needed
    4) If you see a grainy background that DOES NOT MOVE when you move your slide,
    RAISE OR LOWER THE CONDENSOR to eliminate the background
    5) If you see images that move when you rotate your OCULAR LENSES, clean the
    ocular lenses with lens paper, and open the IRIS DIAPHRAGM to let in a bit more light

    IF YOU STILL CANNOT SEE BACTERIA MOVING, DO NOT MAKE A FRESH SLIDE UNTIL YOU
    CHECK WITH YOUR INSTRUCTOR
    Microscopy

    CLEAN UP
     Submit Lab Data Sheets – front lab bench

     Hanging-Drop Depression Slides – Disinfectant Beaker on


    Hanging-Drop Cart
     Simple Stain Slides – Sharps Bin (red box on tabletop)
     Box of clean, unused slides – Label with your name and
    place in drawer at work station
     Stains – Stain Waste Jug in Fume Hood
     Test Tubes – Remove labels and place in rack next to
    autoclave
     Paper Towels – Trash

     Disinfect work area with disinfectant in spray bottle, wipe


    with paper towels.
     Clean Microscope ocular and objective lenses with Formula
    409 and lens paper.
     Clean Microscope stage, mechanical arms, and adjustment
    knobs with Formula 409 and paper towels
     Check that the Microscope 4x/Scanning Objective is in the
    downward position

     Lab Coat, Goggles – Plastic bag labeled with your name

     Gloves – “Gloves Only” bin beside sink


     Wash hands

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