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    Urine Sediment Preparation

    Uploaded by

    Anas Shwbk
    رواسب بولية ٢

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Urine Sediment Preparation

    Uploaded by

    Anas Shwbk
    90 views6 pages
    رواسب بولية ٢

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

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    رواسب بولية ٢

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    90 views6 pages

    Urine Sediment Preparation

    Uploaded by

    Anas Shwbk
    رواسب بولية ٢

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 6

    Urine Sediment Preparation

    Year Group: BVSc3 +


    Document number: CSL_L05
    Equipment list:
    Urine Sediment Preparation

    Equipment for this station:


    • Urine sample
    • 5ml or 1ml disposable conical tip centrifuge tubes
    • Disposable plastic pipettes
    • Centrifuge
    • Stain (Kova™ or Sedi-stain™)
    • Microscope glass slide
    • Microscope slide coverslips
    • Paper towel
    • Gloves

    Considerations for this station:


    • Centrifuges can be dangerous – make sure you know how to use it
    properly. Always use the table top centrifuge on a flat, stable
    surface and do not try to open the centrifuge while it is in
    operation. For more information please refer to General Risk
    Assessment form ‘Centrifuge CSL_R04’ (in the CSL)
    • Microscope slides must be disposed of in a sharps bin. Take care
    when handling glass slides and cover slips. Apply gentle pressure
    when placing a cover slip to a microscope slide. For more
    information please refer to General Risk Assessment form
    ‘Microscope slides CSL_R01’ (in the CSL)
    • Make sure you are familiar with ‘CSL_I02 Lab Area Rules’: wear a
    correctly fastened lab coat/scrub top, mop up any spills and spray
    work surface with 1% Virkon. Wash hands in the hand wash sink.

    Anyone working in the Clinical Skills Lab must read the ‘CSL_I01 Induction’ and
    agree to abide by the ‘CSL_I00 House Rules’ & ‘CSL_I02 Lab Area Rules’
    Please inform a member of staff if equipment is damaged or about to run out.
    Clinical Skills:
    Urine Sediment Preparation

    1 2 3

    Urine sediment preparation is Place the tube into the Centrifuge the sample for 5
    undertaken to identify cells, centrifuge. minutes at 450g
    casts, crystals, and/or Whatever type and size of (approximately equivalent to
    microorganisms. centrifuge, remember to 1500-2000 rpm depending
    Mix the urine sample by balance it correctly and on the centrifuge used).
    gently inverting the container. secure the lid.
    Using a disposable plastic
    pipette, transfer 5 ml of urine
    to a conical tip centrifuge
    tube.
    In practice, there may be 1ml
    or 5ml conical tubes to use.

    4 5 6

    Once the centrifuge has Add one drop of the stain to Re-suspend the sediment in
    stopped, remove the tube. the tube, using a pipette. the supernatant by gently
    Remove the supernatant swirling or “finger-flicking”
    (free fluid at the top of the the tube.
    sample) with a plastic
    pipette or by decanting.
    Keep approximately 0.5 ml
    of supernatant in the tube.
    Take care not to disrupt the
    sediment “pellet” whilst
    removing the supernatant.
    Clinical Skills:
    Urine Sediment Preparation

    7 8 9
    C D

    Use a pipette to transfer one Place a coverslip over the Decrease the intensity of the
    drop of the reconstituted sample. When handling a microscope light by lowering
    sediment to a microscope coverslip, hold it at one the condenser (dial C) and
    slide. corner as shown below. closing the iris diaphragm (D).

    10 11

    Place the covered slide on Microscopic examination of


    the stage of the microscope urine sediment can reveal
    and perform examination the presence of cells, casts,
    using the low power (x10) crystals, and/or organisms.
    first and then the high The photograph above
    power (x40) lens. shows numerous calcium
    oxalate crystals.
    Resetting the station:
    Urine Sediment Preparation

    1. Dispose of glass slides and coverslips in a sharps bin


    2. Dispose of plastic pipettes in a clinical waste bin
    3. If using glass centrifuge tubes dispose in a sharps bin;
    if using plastic centrifuge tubes dispose in a clinical
    waste bin
    4. Securely close the urine pot
    5. Wipe up any spills and leave the area clean and tidy
    6. Anything contaminated with urine (e.g. tissue, paper
    towel, etc.) must also be disposed of in a clinical
    waste bin

    Station ready for the next person:

    Please inform a member of staff if equipment is damaged or


    about to run out.
    I wish I’d known:
    Urine Sediment Preparation

    • A urine sediment can be prepared for examination without


    the addition of a stain. Follow the instructions in this
    booklet but omit step 5.

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