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    100mL・30mL: For pluripotent stem cell stirred suspension culture

    Uploaded by

    Rupal Charaniya Hirani
    This document describes single-use bioreactors for pluripotent stem cell culture. It summarizes bioreactors in 100mL and 30mL sizes that use gentle mixing to form cell aggregates in stirred suspension culture. The bioreactors are made of plastic and gamma radiation sterilized. They are used with a 6-channel magnetic stirrer at low speeds of 20-120rpm to minimize stress on cells during expansion and differentiation.

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    100mL・30mL: For pluripotent stem cell stirred suspension culture

    Uploaded by

    Rupal Charaniya Hirani
    48 views2 pages
    This document describes single-use bioreactors for pluripotent stem cell culture. It summarizes bioreactors in 100mL and 30mL sizes that use gentle mixing to form cell aggregates in stirred suspension culture. The bioreactors are made of plastic and gamma radiation sterilized. They are used with a 6-channel magnetic stirrer at low speeds of 20-120rpm to minimize stress on cells during expansion and differentiation.

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    bioreactor

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    This document describes single-use bioreactors for pluripotent stem cell culture. It summarizes bioreactors in 100mL and 30mL sizes that use gentle mixing to form cell aggregates in stirred suspension culture. The bioreactors are made of plastic and gamma radiation sterilized. They are used with a 6-channel magnetic stirrer at low speeds of 20-120rpm to minimize stress on cells during expansion and differentiation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    48 views2 pages

    100mL・30mL: For pluripotent stem cell stirred suspension culture

    Uploaded by

    Rupal Charaniya Hirani
    This document describes single-use bioreactors for pluripotent stem cell culture. It summarizes bioreactors in 100mL and 30mL sizes that use gentle mixing to form cell aggregates in stirred suspension culture. The bioreactors are made of plastic and gamma radiation sterilized. They are used with a 6-channel magnetic stirrer at low speeds of 20-120rpm to minimize stress on cells during expansion and differentiation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Single use bioreactor series

    For pluripotent stem cell stirred suspension culture

    Made in Japan

    100mL・30mL
    EB formation
    in
    stirred suspension
    culture

    Cell expansion
    Differentiation

    PAT. pending

     Low share stress agitation


     Aggregates formation by gentle mixing
     Plastic, gamma radiation sterilized

    6ch magnetic stirrer

    For 100mL, 30mL

    Very low heat diffusion

    20-120rpm

    This research is granted by the Japan Society for the Promotion of Science (JSPS) through the
    "Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)".
    Application
    Viable cells Cell line ：253G1（human iPS cell）
    Medium ：mTeSR1 (STEMCELL Technologies)
    120
    × 10000 cells/mL

    100
    Culture condition*
    80
    60 • Volume 100mL
    • Agitation 40rpm
    40
    • Temp. 37℃
    20 • pH 7.2
    0 • DO 40% (Air saturation）
    0 1 2 3 4 5 6
    * with cell culture system “Bio Jr. 8” (ABLE)
    Day

    Reference
    1: Matsuura K Fabrication of mouse embryonic stem cell-derived layered cardiac cell sheets using a
    bioreactor culture system. PLoS One. 2012;7(12):e52176.
    2: Matsuura K Creation of human cardiac cell sheets using pluripotent stem cells. Biochem Biophys Res
    Commun. 2012 Aug 24;425(2):321-7.

    Product information
    Product name Cat. No.

    Single use bioreactor for 100mL BWV-S10A

    Single use bioreactor 30mL BWV-S03A

    6ch magnetic stirrer BWS-S03N0S-6

    Contact Us

    日本語: http://www.able-biott.co.jp/
    English: http://www.able-biott.co.jp/en/
    Biott corporation Head office

    6-10, Nishigoken-cho, Shinjuku-ku, Tokyo, 162-0812, Japan


    Phone: +81-3-3260-0415
    Fax : +81-3-3260-0407

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