1.1.3 Study - Applied Science and Technology (Study Guide)

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1.1.

3 Study: Applied Science and Technology Study Guide


AP Environmental Science Name:
Date:

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Use this study sheet to keep track of the important concepts covered in this study. If
you need help answering any of the questions, check with your instructor. Save
these study sheets for review when preparing for quizzes and tests.

Record key term definitions in the table.

Term Definition

acoustics

applied science

biostimulation

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biotechnology

climate

ecosystem

engineering

environmental DNA (eDNA)


surveillance

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gel electrophoresis

geographic information
system (GIS)

Global Positioning System


(GPS)

invasive species

negative (–) control

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positive (+) control

polymerase chain reaction


(PCR)

remote sensing

system

technology

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topography

Studying Earth's Systems

1. Fill in the term for each component of an ecosystem described here.

Component of an Description
ecosystem

The structure of Earth, including its rocks and


soils

The features and elevation of a land area

The weather conditions that prevail in an area


over a long period

Animals, plants, and other living things

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Studying Earth's Organisms

2. For each type of technology, list the example given in the study.

Technology 1: GPS tracking Technology 2: GIS and satellite imagery

Example: Example:

Technology 3: Drone surveillance Technology 4: Acoustic analysis

Example: Example:

Solving Environmental Problems

3. Explain why biostimulation could be considered less invasive for an ecosystem


than many other remediation techniques.

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Biotechnology: Tracking Invaders

4. Describe an invasive species in terms of the three criteria listed here.

Criteria Description of invasive species

Reproduction

Predators

Native species

On the Trail of eDNA

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5. List three methods scientists use to identify species and estimate the number of
fish in a body of water.

Methods used to identify species and estimate their numbers

Identifying Invaders: How It's Done

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6. Put these steps in order by numbering them from 1 to 4.

Step Process

DNA is fragmented using restriction enzymes.

The banding patterns of different organisms are compared with a


positive sample.

The polymerase chain reaction amplifies DNA so it can be analyzed.

DNA fragments are placed in a gel medium with an electric current


running through it.

7. Explain the functions of the negative control lane and the positive control lane.

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Testing Three Sites for Silver Carp

8. Use the table to record the bands from the gel electrophoresis.

North Shore Chicago site 1 Calumet site 1 Negative Positive


site 1 control control

Testing the Remaining Sites for Silver Carp

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9. Use the table to record the bands from the gel electrophoresis.

North Shore Chicago site 2 Calumet site 2 Negative Positive


site 2 control control

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10. If fish move from upstream to downstream, and site 2 is upstream and site 1 is
downstream, which of the following represents the least likely result?

Result

eDNA is found at site 1 but not site 2.

eDNA is found at site 2 but not site 1.

eDNA is found at both site 1 and site 2.

Examining the Results: Silver Carp

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11. Use the table to combine the results from the gel electrophoresis for the first and
second sets of sampling sites.

North North Chicago Chicago Calumet Calumet Negative Positive


Shore Shore site 1 site 2 site 1 site 2 control control
site 1 site 2

12. Silver carp DNA was found at North Shore site 1 and Chicago site 1, but not at
North Shore site 2 or Chicago site 2. Explain how this could be true.

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Testing Three Sites for Bighead Carp

13. Use the table to record the bands from the gel electrophoresis.

North Shore Chicago site 1 Calumet site 1 Negative Positive


site 1 control control

14. Explain why it's necessary to perform separate tests for silver carp and for
bighead carp.

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Testing the Remaining Sites for Bighead Carp

15. Use the table to record the bands from the gel electrophoresis.

North Shore Chicago site 2 Calumet site 2 Negative Positive


site 2 control control

16. Based on your initial analysis, are bighead carp a larger or smaller threat to Lake
Michigan than silver carp? Why?

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Examining the Results: Bighead Carp

17. Use the table to combine the results from the gel electrophoresis for the first and
second sets of sampling sites.

North North Chicago Chicago Calumet Calumet Negative Positive


Shore Shore site 1 site 2 site 1 site 2 control control
site 1 site 2

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18. What are some possible hypotheses to explain why bighead carp do not show up
in eDNA surveillance to the extent that silver carp do? Check the boxes of reasonable
hypotheses about the differences between the two fish species.

Step Process

There are more silver carp than bighead carp.

Bighead carp do not leave eDNA as easily as silver carp do.

Silver carp started colonizing the rivers before bighead carp


did.

Bighead carp are less likely to die at a young age than are
silver carp.

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