Operating Manual DRS2000 ENG PDF

Tissue-Tek DRS 2000
Automatic Slide Stainer
r ating
pe
O nual
M a
IMPORTANT NOTICE
If this equipment is used in a manner not
specified by the manufacturer, the protection
provided by the equipment may be impaired.
© 20 Sakura Finetek USA, Inc.
All Rights Reserved

Printed in U.S.A.
Manufactured for:
Sakura Finetek U.S.A., Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 103-0023, Japan
Sakura Finetek Europe B.V., !6!LPHENAANDEN2IJN.,
Made in Japan
0000030-01 5HY(
TABLE OF CONTENTS
Section Page
1 INTRODUCTION
General Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1
Physical Characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2
Principles of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.6
Safety Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.6
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.7
2 INSTALLATION
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
Environmental Factors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.3
Instrument Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.5
External Connection Terminals and Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.7
Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.8
3 EXPLANATION OF SOFTWARE AND CUSTOMIZATION OF SETTINGS
Station Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.2
Setting a Start, End, or Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.2
Setting a Programmed End Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.3
Converting a Wash Station to/from a Solution Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.3
Adding a Solution or Staining Method Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Deleting a Solution or Staining Method Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Assigning Station Positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.5
Editing a Staining Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.6
Explanation of Function Keys (for the Edit Screen) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.7
Copying a Staining Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.7
Utility Operations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.8
Error Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.9
4 ROUTINE OPERATION
Starting a Staining Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.1
Starting a Staining Program in the Continuous Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2
Starting a Staining Program in Batch Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2
Operations During Staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.3
Adding Basket Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.3
Monitoring a Staining Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.3
Interrupting or Canceling a Staining Process. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4
Checking the Endtime for a Staining Run in Progress . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.5
End of a Staining Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.5
Revised 3/30/2010 iii

Section Page
5 TROUBLESHOOTING
Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.2
6 CARE OF INSTRUMENT
General Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Daily Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Exterior/Interior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Bi-weekly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Replacement of the Activated Carbon Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Monthly Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Drying Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Solution Reservoirs and Slide Baskets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
Reservoir Trays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2
Water Supply Strainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2
7 SERVICE AND REPLACEMENT PARTS
Service Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
When You Have a Problem. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
Where to Call for Service. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.2
Appendix A STAINING RECORD
Sample Staining Record. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A.3
iv Revised 3/30/2010
INTRODUCTION Section 1
General Description The DRS 2000 consists of four major components:

• the control panel, through which all operations are
The Tissue-Tek® DRS™ 2000 Automatic Slide Stainer is controlled
an automated, random-access stainer which carries out • two-levels of solution reservoirs including wash stations
multiple staining protocols simultaneously . The DRS 2000 and one dryer station located on the lower level
(Fig 1-1) features a two-level design which ef ficiently moves
• the robotic arm, which is the transport mechanism for the
slides to various solutions for optimal performance.
slide baskets
The DRS 2000 software is programmable for up to twenty • fume control system, which uses an activated carbon
different staining methods, accommodating routine H & E, filter, for ef fectively removing harmful vapors from inside
gyn, non-gyn, and a variety of special stains or any other the instrument
user-defined protocol. The operator can program the
instrument to begin staining in either a batch or continuous To begin a staining process, the operator places up to two
mode. The batch mode does not allow another process run slide baskets into a designated start station located on the
to start until the previous run has finished. In continuous lower portion of the instrument. Either a batch or continuous
operation, the user may add basket sets in a random-access mode and the desired method may be selected. The robotic
mode, maximizing efficiency and throughput. arm picks up the basket set and sequentially moves the
baskets to the programmed stations. In the continuous
mode, as new baskets are added, the robotic arm will pick up
these additional baskets and move them to their
programmed stations accordingly . The closed-system
design, along with the ventilation system, ensure an
essentially fume-free environment outside of the DRS 2000.
Figure 1-1
Front view of the DRS™ 2000
(cover closed)
Revised 3/30/2010 1.1

INTRODUCTION

Physical Characteristics

Control Panel (Figure 1-2)
The control panel contains the screen display and the
keypad which the operator uses to communicate with the
instrument software. The keypad consists of five function
keys , numeric keys , arrow keys , and a power ON
indicator . The online display of fers the operator visual
information about the status of current operations.

Figure 1-2

Reagent Reservoir Compartment (Figure 1-3)
The reagent reservoirs are arranged on a two-level design.
There are thirteen reservoirs positioned on the upper level
and fourteen on the lower level , plus one drying
station . The lower level accommodates all wash, start,
and end stations. A maximum of five and a minimum of two
wash stations can be used at one time, depending on the
desired set-up. One or two start stations and up to three end
stations may be set as desired. Each reservoir has an
individual lid and handle for ease of filling and transporting.
The reagent capacity is 650 mL; however , two lines molded
on the inside of the reservoirs designate either 650 mL or
Figure 1-3 620 mL. The minimum volume of solution needed for two full
slide baskets is 620 mL. Numbers located on the platform
dividing the levels identify the appropriate stations .
1.2 Revised 3/30/2010

Robotic Arm (Figure 1-4)
The robotic arm can accommodate up to two baskets
of
twenty slides each, at one time. Each basket is secured on
an adapter by a metal hook . The function of the
robotic arm is to move slide baskets to specific stations
based on the user-defined programs. The arm moves on an
x and z axis and ef ficiently carries baskets to the
programmed stations.

Figure 1-4
Fume Control (Figure 1-5)

The activated carbon filter is part of an ef fective fume
control system . As air circulates throughout the inside of
the instrument, fumes generated from various solutions are
captured and extracted by the carbon filter . The fume
Figure 1-6
control housing can be accessed through the lower level of
the instrument, behind the reagent reservoirs. A gasket and
protective cover secures the filter and provides adequate
venting of fumes. It is recommended that the carbon filter
be replaced after two weeks with routine operation of the
stainer.
Figure 1-5
Revised 3/30/2010 1.3

INTRODUCTION
Leveling Feet/Casters (Figure 1-6)

The DRS 2000 has four adjustable feet at each corner of
the instrument to ensure proper leveling. Using two people,
gently tilt the instrument onto one side and rotate the
leveling caster clockwise to decrease the height of the
instrument and counterclockwise to increase the height.
Check all leveling casters to ensure proper positioning of
the instrument.
Figure 1-6 2

Power Switch (Figure 1-6, Figure 1-7)

The power switch is located on the lower , right side of
the instrument. When the switch is moved to the ON
position the green light on the display panel is illuminated.
Brightness/Contrast Dial (Figure 1-6, Figure 1-7)

This dial controls the brightness and the contrast of the
control panel screen.

Figure 1-7
External Connection Terminals (Figure 1-8)

The DRS 2000 has the ability to be connected to an
uninterruptible power supply unit (UPS) and/or an
external alarm device (Not supplied with the instrument).
See page 2.7 for a description of installation procedures.
Figure 1-8
1.4 Revised 3/30/2010

Rear of Instrument (Figure 1-9, Figure 1-10)
The rear of the DRS 2000 shows the inlet for the power
cord on the bottom, right-hand side of the instrument (1-
9). The other end of the cord attaches to an appropriate AC
electrical outlet. The water supply port and the water
supply adjustment valve are both located on the left-
hand side of the instrument (Fig. 1-10). The adjustment
valve is used to increase or decrease the pressure of the
water flowing to the instrument. The drain hose port
allows for suf ficient draining of the DRS. A water supply
hose and drain hose are provided in the accessories for
initial setup.
Figure 1-9

Figure 1-10
Revised 3/30/2010 1.5

INTRODUCTION
The solution, wash, start, and end stations can be

Principles of Operation configured in various arrangements. However , start, end,
and wash stations may only be set on the lower level of the
The DRS 2000 is an automated slide stainer designed instrument. A dryer , fixed at the lower , right corner of the
for use in both the histology and cytology laboratories. Its instrument, can be set at the desired temperature in order
operations include staining of tissue sections mounted on to dry slides and remove residual paraf fin prior to staining.
glass slides, frozen specimens, and cellular specimens, Upon completion of staining methods, the operator is
either non-gynecological or gynecological. alerted by an audible alarm to remove the baskets from the
A microprocessor is responsible for controlling the end station(s).
movement of the robotic arm. It carries the slide baskets to
the appropriate stations, designated by the programs saved
in memory.
Twenty user-defined programs, performing up to fifty Safety Features
steps each, can be set and stored in memory. The operator
also has the flexibility of creating user-defined names for z The DRS 2000 is designed to ef fectively remove fumes
both solution and stain methods to personalize all staining generated from harmful solutions. It uses an activated
operations. carbon filter to purify the air that it releases from within
The staining operations can be performed in either a the instrument.
batch or continuous mode. z Access to the upper level of the stainer is prohibited
In a continuous mode of operation, the user has the during staining by the instrument cover . If the cover is
ability to program one of three options at each station for opened during operation, the audible alarm sounds and
optimum accuracy. a message appears to close the cover. This protects the
operator and the robotic arm from potential harm.
z The instrument contains an alarm that sounds whenever
Exact [z]: Staining time for that step will be performed
there is an error condition that stops or interrupts
exactly as programmed.
operation.
Delta [S]: Staining time may be extended to some z The instrument will alert the operator with an audible
degree but no more than 20% longer than the alarm when any of the end stations are full and the next
programmed time. basket is waiting to complete its run.
z The DRS 2000 software contains various messages that
Infinite [∞ ]: Staining time for that step may be extended inform the user of its current status.
indefinitely.
z If a solution has exceeded its use limit, a message will
The above accuracy settings allow the software to appear to inform the user to change the solution(s) after
determine time critical steps and move baskets as all staining runs are completed.
efficiently as possible. Consistent stain quality results from
this flexibility. Each station can also be individually set for
agitation. Agitation is referred to as a gentle up and down
movement of the robotic arm in a reagent reservoir . (Under
the utilities function, speed, frequency , and amplitude of
agitation can be selected). The [MIX+] key enables the
operator to set the agitation ON or OFF for each station.
The user can also choose an enhanced wash option at any
station. An enhanced wash can be set to avoid excess
staining after a high concentration solution is used. These
key features enable the user to edit a program to best meet
their individual staining needs.
1.6 Revised 3/30/2010

Specifications Continuous Operation:
Basket sets can be continually loaded on demand as the
start station(s) are available for loading.
Power Required:
Programs/Methods:
North America
Model Number: 4929 115 VAC ±10%, 5.0 amps, Up to 20 programs can be stored in memory
60 Hz Up to 50 steps can be programmed for each method
Europe Solution Names:

Model Number: 4930 220/240 VAC ±10%, 0.3 amps, Up to 100 solution names can be stored in memory
50/60 Hz
4931 220/240 VAC ±10%, 2.5 amps, Staining Method Names:
50/60 Hz (with dryer) Up to 50 staining method names can be stored in memory
Dimensions: Facilities:
Width—115.0 cm (45 in.) Water Supply: Tap water
Height— 66.0 cm (26 in.) Water Pressure:
Depth— 49.0 cm (19 in.) Dynamic Pressure: 0.098 to 0.441 MPa
(approx 1.0 to 4.5 kgf/ cm2)
Weight: Max. Static Pressure: 0.833 MPa
101 kgs (223 lbs) (8.5 kgf/ cm2)
Water Temperature:
Operating Conditions: Less than 30°C (86° F) [not freezing]
Temperature — 10° C to 40° C (50° F to 104° F) Drain Type:
Relative Humidity — 30% to 85% (noncondensing) Method — Gravity drain
Atmospheric Pressure — 70 to 106 KPa Capacity — More than 15 liters (3.96 gal)/minute
(10.15 to 15.37 psi)
Safety Standards:
Capacity/Fill Volumes: Complies with: UL 3101-1, 1st ed.
CAN/CSA-C22.2 No. 1010.1-92
Solution Reservoirs (total) — (26)
EN 61010-1:93- A1:92 + A2:95
Wash Reservoirs — (5)
European standards for CE Marking.
Dryer Stations — (1)
Fill level/solution reservoirs — 620 - 650 mL
Batch Operation:
Up to 40 glass slides in two baskets
(2 baskets = 1 basket set)
Revised 3/30/2010 1.7

Section 2
INSTALLATION
General Information Unpacking
This section provides information on selecting a proper 1. Unpack the instrument by removing the large nails along
location, unpacking, and installing the T issue-Tek® DRS™ the bottom of the shipper , then lift the shipper of f the
2000 Automatic Slide Stainer . A T issue-Tek® instrument pallet. Remove all unpacking materials and accessory
representative or a certified Biomedical Equipment boxes prior to moving the instrument off the wooden base.
Technician should perform the installation. The instrument
CAUTION: The instrument is very heavy and large;
must be installed correctly to ensure proper operation and
therefore, it is strongly recommended that it be lifted
service. Read this complete Operating Manual before
and transported by at least two people.
attempting to operate the instrument. Follow all instructions
carefully. 2. After the instrument is unpacked, it is suggested that the
The DRS 2000 is a precision instrument and must be power cord, water supply hose, and drain hose be
handled accordingly. Rough handling or dropping of the secured at the rear of the instrument before placing on a
instrument will disturb or damage internal components. bench or counter top. (Refer to page 2.5, Instrument
Always handle the instrument with care. Setup, for detailed instructions).
3. Carefully lift it into place on a firm, level surface in the

Environmental Factors designated work area. Be sure that each leveling foot fully
contacts the benchtop and that each foot has been
properly adjusted insuring that the instrument is level.
Environmental factors influence the selection of a proper
Turning the height adjustment feet clockwise will lower the
location for the DRS 2000. As with all sensitive electronic
level and turning the feet counterclockwise will increase
instruments, prolonged exposure to excessive humidity and
the level of the instrument.
temperature should be avoided. T emperature and humidity
should be held relatively constant to obtain the highest
WARNING: PLACE THE INSTRUMENT IN A WELL
degree of operating stability. The ambient temperature range
VENTILATED AREA.
for operating the instrument is 10° C to 40° C (50° F to 104°
F). The ambient operating humidity range is between 30% to
4. Remove all packing material from inside the instrument.
85% relative humidity.
Make certain that all styrofoam is removed from around
Locate the instrument in a well-ventilated area, avoiding the base of the robotic arm. (Refer to page 2.6, Step 5.,
exposure to corrosive vapors or temperature extremes. for instructions on removing the bracket from the robotic
Avoid proximity to direct sunlight, open windows, ovens, arm).
open flames, hot plates, radiators, and dry ice baths. A void
proximity to any instrument that consumes a high voltage or
large current, including large refrigerators and ovens. The
bench must have a firm level surface capable of supporting at
least 130 kgs (287 lbs.) of weight. Be sure the instrument
will be located near a power source that meets the electrical
requirements (voltage and amperage) specified on the rating
label located on the rear of the instrument. The power
receptacle must be grounded and should be a clean, noise-
free, dedicated line. The water supply port is located on the
back of the instrument at the lower left corner . The location
of the DRS 2000 should be positioned to the left of the
external water source making certain the water supply hose
can be properly connected to the instrument and external
water supply.
Revised 3/30/2010 2.1

INSTALLATION
5. The accessory box includes all items necessary for routine

operation. Please verify that all of the following
accessories are included with the instrument. Refer to the
next two pages for a photo of each accessory.
• Reagent reservoirs — 26
• Wash reservoirs — 5
• Reagent trays (for upper level) — 2
3. • Reagent tray (for lower level) — 1
• Wash reservoir grommets — 10 (5 installed)
3. • Wash station plug — 3 (2 installed)
3. • Wash nozzle — 5 (3 installed)
3. • Reservoir lids — 26
3 • Lid for drying station — 1
3 • Slide baskets — 6
3 • Basket hooks — 6
3. • Basket adapters — 3
3. • Drain hose — 1
3. • Drain hose clamp — 1
3. • Drain elbow assembly— 1
(includes drain elbow , connecting hose, and two
connecting hose clamps)
• Water supply hose set — 1
(includes drain hose, washer/filter, and washer)
• Activated carbon cartridge filter — 1
• Filter gasket and metal cover — 1 each
3. • Fuse — 1
3. • Power cord — 1
3. • Labels (Start, End, and PE)
3. • Operating Manual — 1
(including W arranty Registration Card — U.S.
customers only)
If any of these items are missing, contact your

Customer/Product Support Representative at Sakura Finetek
at (800) 725-8723.
If there is any visible shipping damage to the instrument or
any item, immediately file a complaint with the carrier , then
notify Sakura directly.
2.2 Revised 3/30/2010

Accessories

Wash Reservoir - 5
Reagent Reservoir - 26

Reagent Reservoir Lid - 26

Basket Adapter - 3
Basket Hook - 6
Wash Nozzle - 5
Slide Basket - 6
Wash Cap - 3
Wash Reservoir Grommet - 10

Drain Elbow Assembly

Drain Elbow
Drain Hose - 1 Connecting Hose
Connecting Hose Clamps
Revised 3/30/2010 2.3

INSTALLATION
Activated Carbon Filter Cartridge -1
Water Supply Hose Set -1

(includes drain hose, washer/filter, and washer)
Filter Gasket for Carbon Cartridge - 1
Power Cord - 1
Metal Cover for Carbon Cartridge -1
Reagent Trays (for upper level) - 2
Drain Hose Clamp - 1
Reagent Tray (for lower level) - 1
2.4 Revised 3/30/2010

3. To install the drain hose, place the drain hose assembly
Instrument Setup onto the drain port and tighten the connecting hose
clamps to ensure hose clamps are properly seated.
For ease of installation, the power cord, water supply hose, Next, the drain hose can be attached to the drain hose
and drain hose should be secured at the rear of the assembly ensuring the clamped end of the hose attaches
instrument prior to placing on a bench or countertop. to the elbow . Tighten the clamp by using a phillips
CAUTION: The robotic arm is secured at the lower left screwdriver. The other end of the drain hose should be
side of the instrument by a metal bracket and must be routed to a floor or sink drain which is capable of draining
removed before turning on power. at a rate of 15 liters (or 4 gallons) per minute.
1. Locate the serial number label found on the left side of the CAUTION: Make sure that the entire length of the
instrument, next to the power cord. W rite the installation drain hose is lower than the instrument drain port. It
date and instrument serial number on the W arranty should be straight and not twisted or looped. Do not
Registration Card found in the front section of this manual. extend the drain hose by coupling it with another
Completely fill out the Warranty Registration Card and mail hose. The end of the drain hose should be at least 4
it back to Sakura for registration. The warranty is valid from inches (100 mm) or higher from the water surface in
1 year after the date of installation. the floor or sink drain.
2. Before attaching the water supply hose to the
instrument, place the plain washer in the curved end of the
hose and place the screen washer (screen first) in the
straight end of the water supply hose. Next, connect the
3. To in
preassembled water supply fitting to the instrument, which
is located at the rear, left side of the unit. The water supply
hose may now be placed on the fitting and secured. The
other end of the hose should be securely fixed to a faucet
or water source at the customer location.
CAUTION: When installing the water supply hose,
make sure it is securely connected to the instrument
and the faucet. Failure to do so may allow water
pressure to loosen the connection and cause overflow.
CAUTION: It is recommended that the water supply to
the instrument be turned OFF when the laboratory is
left unattended, i.e., overnight or weekends. 4. In order for water to begin flowing to the wash reservoirs,
the water supply knob must be opened. T urning the
knob counterclockwise will open the valve and clockwise
will close the valve. When adjusting the water flow for the
first time, please follow the guidelines below:
Number of wash Number of

stations used turns
5 3 turns
4 2 turns
3 1/2 turn
2 1/4 turn

Revised 3/30/2010 2.5

INSTALLATION
5. To release the robotic arm, cut the tie wrap and gently 8. The solution reservoirs may be placed in the upper and
raise the arm . Completely remove the two screws lower sections of the instrument. The wash reservoirs
securing the bracket to the left wall . Next, loosen the are pre-set on the lower level. To change a wash station
two screws securing the bracket to the tower . Lift the to/from a solution station, please refer to Section 3, page
bracket upward then remove it . Retighten the two 3.3. Check to be sure all reservoirs are properly seated.
screws on the tower . Labels on the upper level designate each station number.
Start and end labels are included in the accessory box
and should be placed on the lower , inside door once all
start/end stations have been determined.
9. The solution reservoirs can now be filled with the desired
reagents. A molded line on the inside of each container
designates the proper fill level. The lower line (620 mL)
can be used as a fill line if two baskets of forty slides will
always be run. The higher line designates 650 mL and
should be used to ensure adequate solution levels if only
one basket is usually run.
10. If the power cord is not already attached, plug the
instrument power cord into the DRS, located on the rear ,
lower right corner of the instrument and the other end
into a dedicated, grounded electrical power source of the
6. The three metal trays enclosed in the accessory box fit proper voltage and amperage.
securely on both the right and left side of the upper level 11. Move the power switch, located at the bottom right side
and along the lower level. When installing the tray on the below the keypad, to the ON position. A small green light
lower level, insert it below the protruding wash nozzles on the control panel will illuminate. W ater is introduced
with the black plastic arrows facing toward you. These into the instrument for one minute upon power up if the
trays ensure the proper placement of the solution water supply hose is properly attached to the instrument
reservoirs. Insert them so that the edges are facing down. external water source, and if the water flow adjustment
valve is in the open position.
7. The activated carbon cartridge, filter gasket, and metal
cover can be installed in the lower , rear section of the NOTE: If water is leaking, close the faucet and the valve
DRS 2000. Make sure that the robotic arm is positioned and verify the connections again.
all the way to the right of the instrument over station 27. 12. The screen on the control panel will display the initial
Release the metal cover in the rear of the unit by pressing Main Menu screen.
the black button on the right. The carbon cartridge can be
installed, making sure that the filter gasket is placed onto
the filter housing first, then the filter and the metal cover
last. To secure in place, put the left end of the filter and ® TM
metal cover in first, then secure the right side by pressing Tissue-Tek DRS 2000
on the black button.
START MONITOR EDIT STATION MORE
Main Menu Screen
2.6 Revised 3/30/2010

2. Connection to an External Alarm Device
External Connection Terminals
If an operational error occurs, the instrument sends an
and Options alarm signal to an external alarm device through the
external alarm output terminals. The external alarm
If desired, the DRS 2000 may be connected to an device connected to these terminals must be able to
uninterruptible power supply unit and to an external alarm meet the following specifications:
device, (not supplied with instrument).
• The external alarm device must have its own power
1. Connection to an uninterrupted power supply unit source
(UPS)
• Rated voltage: 30 Vrms., less then 60VDC (24VDC is
To connect the UPS unit, connect the power failure input recommended)
terminals of the instrument to the output terminals of the
UPS using a biaxial cable (20 A WG, approximately 3 • Rated current: more than 10mA, less than 1A
meters in length). Next, plug the instrument power cord • Connecting cable: Biaxial cable, approx. 10 meters in
into the power output receptacle of the UPS. Turn on the length, 20 AWG
instrument power switch, and then change the [UPS
Connect] default in the System Setup screen from [NO] • Output terminal structure: Non-voltage relay (C
to [YES]. contact)
When the instrument detects a power failure signal The instrument can transmit the following error signals to
coming from the UPS, it operates as follows: the external alarm device:
• The instrument will continue normal operation for 5 ERROR NO. 1 [System Error]
minutes after receipt of the power failure signal; ERROR NO. 2 [Power Failure]
however, the heater of the drying station will be ERROR NO. 3 [Memory Error]
disabled. ERROR NO. 10 [WARNING: Close the cover.
Pressing exit will abort all runs.
• If power is restored within 5 minutes, the instrument Press resume to continue staining.]
will continue normal operation. The drying station will ERROR NO. 11 [Robotic arm does not work normally .
then be operational again. Refer to the manual.]
• If the power failure continues over 5 minutes, the Status of output terminal circuit:
instrument will stop operation while all basket sets
are immersed in their respective stations. Terminal Normal Condition Error Output Conditions
The UPS should have the following specifications: 3-4 Short Open
3-5 Open Short
• Back-up capacity: 200 VA, more than 10 minutes
• Power failure output terminal: Non-voltage relay
output, or open collector transistor output
During power on: Short signal 3. Duct Connection Adapter (optional)
During power off: Open signal The optional duct connection adapter (part number A-
AK23-0631-01) is designed to connect the instrument to
an external air duct in order to exhaust the fumes. The
outer diameter of the adapter is 38 mm.
If the duct from the instrument to the air connection
adapter and the air duct is used, a ventilation fan can be
installed, which is external to the instrument, for more
effective fume control in the laboratory.
Revised 3/30/2010 2.7

INSTALLATION
Precautions
There are several precautions that must be observed
before operating this instrument.
1. Check the fill level of each solution reservoir to ensure
the specified volumes. Overfilling the reservoirs may
cause an overflow and too little solution volume may be
insufficient for adequate staining.
2. Check that the water supply and drain hose are properly
attached. The water supply hose should be securely
connected to the water supply port on the rear, left side of
the instrument and the faucet connection at the sink.
The drain hose should be connected to the instrument at
the rear, left side and checked to be sure it is draining
properly. If the drain hose is not installed correctly , water
may overflow inside the instrument.
Verify the following:
— the full length of the drain hose should be lower than
the drain port
— the drain hose should be straight, not twisted
— the end of the drain hose should be at least 4 inches
or higher from any fluid surface in the sink or drain
3. Check to be certain that the bracket, securing the robotic
arm is removed and the arm can move freely throughout
the instrument.
2.8 Revised 3/30/2010

EXPLANATION OF SOFTWARE/CUSTOMIZATION Section 3
General Information When the DRS 2000 instrument is first turned on, the
Main Menu is displayed. The Main Menu screen is the
The Tissue-Tek® DRS™ 2000 Automatic Slide Stainer central screen from which all set up, programming, and utility
contains software that can be customized for the routine functions are accessed. T wo groups of functions are
work and procedures performed in the laboratory . This accessed through the Main Menu. Pressing the [MORE] key
section explains the various operating modes and provides allows the operator to access both groups.
information on customizing the software. Many of the The [START] function key accesses the Start Process
procedures in this section will be used upon initial screen, which allows the user to select a method and begin
installation. However , detailed information on the staining. Once the method has started, the Process Monitor
progression of screen displays and prompts is given here; screen appears on the display , which indicates where each
therefore, this section should be read thoroughly before basket set is located. The [MONIT OR] key cannot be
beginning routine operation. Once you are familiar with this activated from the Main Menu screen unless the instrument
information, Section 4, Routine Operations, can be used as is actively staining. The [EDIT] key accesses the Staining
a quick reference guide for everyday use. Method List screen. Through this screen, names can be
added/deleted and methods can be programmed. The
[STATION] key allows the operator to choose user-defined
and/or factory set solutions. In this screen, the user can
verify or modify the solution names and verify or reset the
solution usage counters. Pressing [MORE] accesses the
second group of functions.
The only function keys on the second group of keys are
the [UTILITY] and the [EXIT] key . The [UTILITY] key
accesses the Utility Function Selection screen, where the
instrument default parameters can be changed and the error
records can be checked. It also enables the operator to set
up the solution configuration from the Station Configuration
screen. When pressed, the [MORE] key will return again to
the first set of functions.
® TM
Tissue-Tek DRS 2000

UTILITY MORE
Main Menu Screen
Revised 3/30/2010 3.1

EXPLANATION OF SOFTWARE
Station Configuration NOTE: The new configuration must be saved before exiting.
NOTE: Start and end stations S1 and E1 will always occupy
The Station Configuration screen allows the operator to the lower station number and S2 and E2 will occupy the
set all solution, wash, start, and end stations, including the higher station number. Default settings for start stations are
programmed end. The programmed end station is a user- S1 at station 26 and S2 at 27. Default settings for END
defined end station which may be set on the lower level of stations are E1 at station 14 and E2 at 15.
the DRS 2000 and may be programmed at any station other NOTE: A minimum of one and a maximum of two start/end
than a wash station. stations may be set. Start/end stations may only be set on
the lower level.
Setting a Start, End, Solution, or Wash NOTE: A minimum of two and a maximum of five wash
Station stations may be set. If you want to reconfigure the wash
stations, you must also do a hardware change in order to
To set a Start, End, or Wash station: either convert a wash station to a solution station, or a
1. Press the [MORE] function key from the Main Menu to solution station to a wash station. (Refer to page 3.3 for
access the [UTILITY] key . Then press the [UTILITY] key detailed instructions).
and the [SETUP] key to display the message,
“INSTRUMENT SETUP.”
2. Press the [ST ATION] key which will display the Station
Configuration screen. The cursor will initially be on the
station labeled “E” (end station) at station 14. The arrow
keys on the keypad will move the cursor to any desired
station location on the upper or lower level; however all
start, end, and wash stations must be positioned on the
lower level of the instrument.
If you would like to change the factory set positions in the
station configuration, use the arrow keys to move to the
desired station location and press the function key(s),
[START], [END], [SOLN], [W ASH], and/or [PGM END] to
complete the solution arrangement. The [PGM END] key
can be accessed by pressing the [MORE] key from the
Station Configuration screen.
1 2 3 4 5 6 7 8 9 10 11 12 13
E1 E2 16 17 18 19 20 W1W2 W3 24 25 S1 S2 DRY
SOLN START END WASH MORE
UTILITY MORE
Station Configuration Screen
3.2 Revised 3/30/2010

3. Pressing [EXIT] will enable you to modify the existing
Setting a Programmed End Station station configuration, [VERIFY] allows you to view the
A programmed end (PE) station may be set on the lower staining programs which will be deleted and [DELETE]
level to allow the operator to set up a user-defined end will delete those programs which are incompatible with an
station in addition to the fixed end station(s). The existing program. If the [DELETE] key is pressed, the
programmed end may be used for the purpose of running station configuration is then replaced according to the
special stains and/or deparaffinizing. modification. The display will return to the System Setup
To position a programmed end Station: Selection screen.
1. Press [MORE] to choose the programmed end from the
Station Configuration screen. Use the arrow keys to
Converting a wash station to/from a
select the desired station location and press [PGM END].
solution station
NOTE: The programmed end does not need to be used;
Once you have selected the desired station configuration,
however, if you choose to edit a method with a
you may or may not need to make changes to remove the
programmed end station, it must first be set up in the
factory set wash stations. The wash stations are set at
Station Configuration screen. Once a PE station is set,
station positions 21, 22, and 23. W ash stations may only be
that station cannot be used for anything other than a PE
set at station positions 21 to 27. A maximum of five and a
station.
minimum of two may be used at one time.
2. After the desired configuration is achieved, press the
[SAVE] function key . The station configuration will be
To transform a wash station to a solution station:
saved in memory.
1. Remove the wash reservoir(s) from the lower level.
The start/end and PE labels provided as an accessory
Turning the wash cap counter-clockwise, remove the
item may be placed on the inside of the lower door
wash nozzle and gasket from the instrument. Replace the
accordingly.
wash nozzle with a wash station plug, and secure the plug
The following section provides some corrective procedures and gasket in place using the wash cap. Next, place a
and explains display messages pertaining to the station solution reservoir at that position.
configuration process.
z If the station configuration is incomplete, a message will To transform a solution station to wash station:
appear to alert you of an error so that you may modify the 2. Follow the procedure in reverse order . Turning the wash
configuration. cap counter-clockwise, remove the wash cap, wash
For instance, if there are no programmed wash stations, a station plug, and gasket. Replace the wash station plug
display message will appear: “A MINIMUM OF TWO WASH with the nozzle and secure all items in place. Install a
STATIONS MUST BE PROGRAMMED ”. Pressing the [EXIT] wash reservoir at this station.
key will return you to the Station Configuration screen and 3. Verify that the station configuration on the display monitor
allow you to verify and correct solution arrangement. reflects the proper wash station positions.
z If there are already edited staining programs in memory ,

and the new station configuration is incompatible with any
of them, the following message will appear:
“THE NEW SOLUTION CONFIGURATION IS INCOMPATIBLE WITH
AT LEAST ONE EXISTING PROGRAM. PRESS EXIT TO
RECONFIGURE, VERIFY TO VIEW OR DELETE TO DELETE
THESE PROGRAMS.”
Revised 3/30/2010 3.3

5. The [BACK SPC] key will delete the last character

Adding a Solution or Staining Method entered. The [SHIFT] key will convert the highlighted
Name character to either upper or lowercase.
This procedure allows you to create and enter user-defined NOTE: The space in front of the letter “a” is used to create
solution or method names. a space in the text.
To edit a solution or method name: 6. Press the [SA VE] key after all characters have been
1. From the Main Menu, press the [EDIT] key . Press the entered. The new solution or staining method name(s)
[MORE] key to access the second group of function keys. are saved into memory and will be recalled when
The [NAMES] key, will allow you to add or delete solution assigning solutions and editing programs. Pressing
or method names. [EXIT] will return you to the Name Creation Selection
screen and pressing [EXIT] sequentially will return you to
2. Press the [NAMES] key to add a solution or method the Main Menu.
name.
3. Press [SOLN+] or [METHOD+] to edit names.
Deleting a Solution or Staining Method

Name
ADD OR DELETE SOLUTION OR METHOD NAMES
To delete an unwanted solution or staining method name:
SOLN+ METHOD+ DELETE REMOVE EXIT 1. Press [DELETE] from the Name Creation screen. Next,
press the [SOLN-] or [METHOD-] key relevant to the
Name Creation Selection Screen name(s) you wish to delete.
2. The display will either read, “DELETE SOLUTION NAME” or
4. Select each character by using the arrow keys to move “DELETE METHOD NAME.”
the cursor to the desired letter , number or symbol and
press [ENTER]. The character will appear next to the 3. Using the up and down ( S T) arrow keys, move the
Solution/Method name at the top of the display. cursor to highlight the name(s) to be deleted and press
the [DELETE] key. To confirm, press [YES] or [NO].
ADD NEW SOLUTION NAME
SOLUTION NAME ? HEM 4. Pressing [EXIT] sequentially will return you to the Main
abcdefghijklmnopqrstuvwxyz Menu.
0123456789!"#$%&'()*+,-./:;
<=>?@[\]^_{I}
ENTER BACK SPC SHIFT SAVE EXIT
Solution Name Creation Screen
3.4 Revised 3/30/2010

or down arrow keys or FUNCTION keys to scroll through
Assigning Solution Positions the Solution selection list.
From the Solution Configuration screen, you can assign or 3. To set the solution usage limit, move the cursor to the
modify solutions in the setup configuration. The solutions “Count” column by using the right arrow key . Enter a
stored in memory , either user-defined or factory set, will be desired number of times you would like that specific
recalled when assigning solutions. Y ou may also use this solution used. You may select a range of (1-999) by using
screen for solution management. The “COUNT” column in the numeric keys. If the solution management option is
this display allows you to set the number of times you would not needed, press the [DISABLE] key to change the
like each station to be used. The first number under the numbers in the “Count” column to [---(---)]. The Solution
count column identifies the “set” number . The number in management option will be disabled for each station
parenthesis increases each time that specific solution is displaying [---(---)]. Pressing the [EXIT] key will return you
used. The instrument will keep track of the number of uses to the Main Menu
and will alert you when a station needs to be changed. This
function can be on or off for each station. The symbol, “_ __” ASSIGN SOLUTIONS TO STATIONS
indicates this function is not being used at a given station. STA SOLUTION COUNT
1 XYLENE ---(---)
ASSIGN SOLUTIONS TO STATIONS 2 XYLENE 1 ( 10)
STA SOLUTION COUNT 3 E-ALC:100% 3 ( 5)
1 XYLENE ---(---) ASSIGN COUNT 0 ALL 0 PRINT EXIT
2 XYLENE 1 ( 10)
3 E-ALC:100% 3 ( 5) DISABLE COUNT 0 ALL 0 PRINT EXIT
ASSIGN COUNT 0 ALL 0 REMOVE EXIT
Solution Configuration and Usage Count Screen
Solution Configuration and Usage Count Screen
NOTE: The function key [COUNT 0] will reset the number

To set solution positions: at that station to 0. The [ALL 0] key will reset all stations
1. From the Main Menu, press the [ST ATION] key. The to 0.
display will read, “ ASSIGN SOLUTIONS TO STATIONS .” Move
the cursor to the desired station number by using the up 4. When a solution has reached its usage limit, the
and down arrow keys and press [ASSIGN]. The Solution message, “A SOLUTION HAS REACHED ITS USE LIMIT. PLEASE
Name Selection screen will appear and the solution list CHANGE THE SOLUTION AFTER ALL STAINING RUNS HAVE
will appear as a window in the upper right corner of the ENDED” will appear. Y ou may change the appropriate
display. stations and reset the “COUNT” column for each station
by pressing [COUNT 0]. If you do not reset the count
ASSIGN SOLUTIONS TO STATIONS SOLUTION LIST
STA SOLUTION COUNT E-ALC: 100% column, after the next staining run is completed, you will
1 XYLENE ---(---) E-ALC: 50% be prompted again to exchange solutions after all runs
E-ALC: 70%
2 XYLENE 1 ( 10) E-ALC: 75% have ended.
NOTE: Many internal components of the DRS 2000 are
ENTER UP DOWN EXIT made of stainless steel. These components have a
protective coating which is resistant to most solvents and
Solution Name Selection Screen reagents used in histological and cytological staining
procedures. Iodine, by nature, will penetrate the
2. Using the arrow keys, scroll through the solution list and protective coating and allow corrosion to occur . To
select the desired solution for a station by pressing enhance the life of the DRS 2000 instrument and its
[ENTER]. The solution name will appear under the components, the use of iodine or any other halogen
column marked “SOLUTION”. You may use either the up salt is not recommended.
Revised 3/30/2010 3.5

3. Once the edit screen is displayed, use the arrow keys to

Editing a Staining Method move the cursor to the desired step and the numeric keys
This section describes the process of creating and editing a to enter the number of the station position. When a
staining method. The edit mode allows you to set up a station number is entered under the column labeled
staining method to best meet your needs by allowing you to “STA”, the corresponding solution from the solution list will
program times, agitation, and other options to enhance stain automatically appear.
quality. Before editing a method, the station configuration NOTE: To program a W ash, Dryer, End, or PE station,
and solution assignment needs to be set up. Please refer to use the function keys labeled [W ASH], [DRYER], [END],
pages 3.2 and 3.4 to complete these procedures if they have and [PE]. Use the [MORE] key to access these functions.
not already been completed.
4. The time may be set from (00’01 to 99’99) for each
To edit a staining program: station. Set the desired time using the numeric keys,
1. From the Main Menu, press [EDIT]. The display will read: press [MORE], then [SAVE].
“SELECT STAIN METHOD.” Any method showing an asterisk
5. The “MIX” column is used to set agitation or an enhanced
“*” next to the name means that it has already been wash at that specific station. Press the [MIX+] key to
edited and saved in memory. display the character “+” to choose agitation at that
NOTE: If you are editing a method for the first time, the
only “*” method will be the factory set HE(Sakura)
*
specific station or “ ” to choose an enhanced wash at that
station. If nothing is displayed, neither agitation or an
method. enhanced wash will take place.
2. Move the cursor to the desired method name by using the
up and down arrow keys and press [EDIT]. The Staining
Program Editing screen is displayed. (If you wish to
create a new staining method name, please refer to page
3.4, Adding a Solution or a Staining Method Name.
STP STA SOLUTION TIME MIX
8 10 E-ALC 00'30" +
9 11 XYLENE 02'00" *
10 12 XYLENE 03'00" +
11 13 DISTILLED WATER 02'00" +
12 E* END STATION _ _ '_ _"
EXACT MIX + WASH END MORE
Staining Program Editing Screen
3.6 Revised 3/30/2010

Explanation of Function Keys Copying a Staining Method
(for the Edit Screen)
To copy one Staining Method in order to create a new
The [EXACT] function key is used for the purpose of method, follow the procedure below:
establishing critical and non-critical process times for a
specific step. Its main purpose is to create flexibility in
process times which ultimately increase throughput. Each 1. From the Main Menu press [EDIT]. The Staining Method
time the [EXACT] key is pressed, the symbol in front of the List screen is displayed.
time in the “TIME” column will change from [z] to [S] to [∞ ],
then will cycle back through the sequence. 2. Use the arrow keys to select the stain method name you
EXACT [z]: The process time at that step will be would like to copy from, then press [COPY].
performed exactly as programmed.
DELTA [S]: The process time for that step may be 3. The display will read, “SELECT STAIN METHOD TO COPY TO.”
extended to some degree but by no more Use the arrow keys to scroll down and select the method
than 20%. you would like to copy to and press [ENTER].
INFINITE [∞]: The process time at that step may be
extended indefinitely to provide maximum 4. To verify, press [ENTER].
flexibility.
If there are no time critical steps, the infinite [ ∞] symbol 5. To modify the copied method, press [EDIT] and follow edit
may be used at all stations for optimum throughput. Exact procedures on page 3.6.
[z] should only be used when the time for a specific solution
should not be modified. The delta [ S] symbol should be
6. Press [EXIT] to return to the Main Menu.
used when a time for a specific station can be lengthened
for no more than 20% of its programmed time.
The [MIX+] key offers three options:
1 If the [+] symbol is shown, the instrument will agitate in
that station based on the parameters set under the
[UTILITY] function. Mix amplitude, frequency , and
speed can be programmed under the [UTILITY]
function.
2 If the [ *] symbol is displayed, that indicates that the
Enhanced Wash feature is set for that step. The
Enhanced Wash feature can be used to avoid excess
staining when using highly concentrated solutions.
Under the [UTILITY] function you may also set the
enhanced wash frequency and speed.
3 The blank field [ ] indicates that neither mixing or an
enhanced wash will be carried out.
Revised 3/30/2010 3.7

3. Move the cursor to the parameter you would like to

Utility Operations change by using the up and down arrow keys, then press
The [SETUP] function key under utility operations of fers the the [SELECT] key. The parameter options will appear in a
user a wide range of user-selected features, which enhance window in the upper right corner of the display.
stain quality. The error log may also be accessed under 4. To modify the parameters, move the cursor to the desired
[UTILITY] by pressing the [ERR LOG] key. parameter setting by using the up and down arrow keys
and press [ENTER]. The default parameter will change to
1. The [UTILITY] function key can be accessed by pressing the selected option. Follow this procedure for each
the [MORE] key from the Main Menu. The functions parameter you wish to change. After all parameter
under [SETUP] enable you to set up parameters for all changes have been made, press the [EXIT] key . A
programs which enhance staining operations. message will appear , “DO YOU WANT TO SAVE SYSTEM
SETUP?” Press the [YES] key to confirm, to cancel, press
2. Press in sequence [UTILITY] Î[SETUP] Î[MODE]. the [NO] key. Pressing [EXIT] sequentially will return you
The Default Parameters screen appears and listed are the to the Main Menu.
following default settings:
1. MIX AMPLITUDE 25 mm
2. MIX FREQUENCY 3 times
3. MIX SPEED 3
4. BASKET LIFTING SPEED 1
5. DRYER TEMPERATURE SET 65°C
6. WATER WASH CYCLE TIME 1min 00 sec.
7. ENHANCED WASH FREQUENCY 3 times
8. ENHANCED WASH SPEED 5
9. UPS CONNECT NO
10. KEY SOUND YES
11. PRINTER BOX CONNECT NO
3.8 Revised 3/30/2010

A description of the parameters and their allowable ranges ENHANCED WASH SPEED:
are as follows: The speed at which the robotic arm moves up and down
during the enhanced wash. Range: (1, slowest - 5, fastest)
MIX AMPLITUDE:
The travel distance of the robotic arm during the up and UPS CONNECT:
down movement of the mixing cycle.
This indicates whether or not the Uninterrupted Power
Range: 10/15/20/25/30 mm.
Supply (UPS) is connected to the instrument. Range:
Yes/No
MIX FREQUENCY:
The number of times the robotic arms moves up and down NOTE: The UPS connect parameter needs to be set at [NO]
during each mixing cycle. Range: (0-10) times. if there is no UPS connected to the instrument. If [YES] is
set, the instrument will enter into the power outage
MIX SPEED: operation mode regardless if a power outage actually
The speed at which the robotic arms move up and down happened.
during mixing. Range: (1-5). (1, slowest - 5, fastest)
KEY SOUND:
BASKET LIFTING SPEED: The operator can select between silent operation or an
audible signal whenever a key is pressed. Range: Y es
The speed at which the instrument lifts the basket set from
(audible)/No (silent).
the solution reservoir. Range: (1, slowest - 5, fastest)
DRYER TEMPERATURE SET:

The drying station temperature, which can be input by the Error Log
numerical keys. Range: (30-65°C in 1° increments)
The [UTILITY] function key can also be used to view any
errors that may have occurred during staining. The Error
WATER WASH CYCLE TIME: Code Log will display a code, which is identified by a
The length of time the water flow to the wash stations number, and will provide a brief description of the problem.
continues after the baskets(s) have left the wash station. If an error has occurred, you can access this display and
Range: (minimum: 30 seconds, maximum: 1 minute and 30 refer to the Troubleshooting Chart, beginning on page 5.1, to
seconds in 1 second increments) review a description of the problem and a possible remedy .
The error codes can be cleared out of the log by pressing
ENHANCED WASH FREQUENCY: the [CLEAR] key.
The number of times the robotic arm moves up and down
during each enhanced wash cycle. Range: (1, slowest - 5,
fastest)
Revised 3/30/2010 3.9

Section 4
ROUTINE OPERATION
3. Open the water faucet at the sink connection to enable
Routine Operation the flow of water to the instrument.
This section describes the basic procedures necessary to To manually open the wash valve to each wash reservoir,
operate the DRS 2000. T o program a staining method or please follow the procedure below:
edit an existing program, refer to Section 3, Explanation of
Software. 1. Press the [MORE] key from the Main Menu to access the
second group of function keys and then press the
[UTILITY] key. The Utility Function Selection screen will
be displayed.
Starting a Staining Process
2. Press the [SETUP] key and then the [W ASH ON] key .
The Water Flow Confirmation screen will appear.
1. Before starting a run, verify the following:
• The water supply and drain hose are securely 3. The water begins flowing into the wash stations and the
connected water supply time begins counting when the “ VALVE IS
• The robotic arm is free to move OPEN” message is displayed.
• The cover and the lower door are properly closed.
4. When the water begins flowing through the rectangular
CAUTION: Check that the water supply hose and the rim of the wash reservoirs, the valve can be turned of f by
drain hose are not kinked and that all connections pressing [CLOSE]. When the [CLOSE] key is pressed,
are secure. the valve shuts off and the water stops flowing to all wash
stations. Once the adjustment is completed, pressing the
2. Turn the power switch to “I” position. The Main Menu [EXIT] key twice will again return you to the Main Menu.
screen will appear.
® TM
Tissue-Tek DRS 2000

UTILITY MORE
Main Menu
Revised 3/30/2010 4.1

ROUTINE OPERATION
Starting a Staining Program in the Starting a Staining Program in Batch Mode

Continuous Mode To begin a method in the Batch Mode, follow the steps
To begin a staining method in the Continuous Mode follow below:
the steps below:
1. Press the [ST ART] key from the Main Menu. Place the
1. Press the [ST ART] key from the Main Menu. Place the basket set to be run in the Start Station labeled, S1.
basket set to be run in the appropriate Start Station
labeled, S1 or S2. 2. Press the [MODE] key to display: “MODE: BATCH” at the
bottom right side of the Start Process screen.
2. If the “ BATCH” message is displayed on the monitor as
follows: “ MODE: BATCH ”, pressing [MODE] key will change The robotic arm gives priority to only one basket set. When
“BATCH” to “ CONTINUOUS” and toggle between the two, staining in the batch mode, you may not add additional
each time it is pressed. In order to run baskets is a basket sets until each run is completed. The total length of
continuous mode, “CONTINUOUS” must be displayed time it will take to run one basket set in a batch mode is
completely dependent on the times programmed for that
3. If the desired method is not currently displayed at the top specific protocol. If you would like to begin staining in the
of the screen, press the [METHOD] key to view your batch mode of operation, you may choose a specific start
options. Use the arrow keys to scroll through the list of step to begin. From the Start Process screen:
methods then press [SELECT] to choose the program
you wish to run. T o start the desired method press the 1. Press the [STEP] key.
[START] key. The robotic arm will pick up the basket set
and move it to the first programmed station. 2. Use the arrow keys to scroll to the desired start step for
that run, open the door and cover, place the basket set in
NOTE: The [MODE] key cannot be selected while the the desired start step, close the door and cover and
instrument is in operation. All baskets must be removed press [START].
before changing modes.
In batch mode operation, the stainer will accommodate up
to 40 slides/run, one basket set.
4.2 Revised 3/30/2010

Operations During Processing Monitoring a Staining Process
During the staining process, the Process Monitor screen is
Adding Basket Sets displayed. The Process Monitor is a visual display which
identifies where all staining runs are located at any given
This section describes how to add additional baskets during time. From the Process Monitor screen the operator can
staining. The [MODE: CONTINUOUS] indication must be make various selections. Additional basket sets maybe
displayed at the bottom right side of the Start Process added, end times for each run may be verified or runs may
screen. This indicates that the instrument is currently in the be aborted. Pressing [MAIN] will return you to the Main
continuous operation mode which allows the operator to Menu where you may perform other operations.
add more baskets.
Each basket set will be moved through the stainer according
To add a basket to the stainer: to the specific staining method selected. A run number is
associated with each basket set and as the robotic arm
1. Press the [ADD] key from the Process Monitor screen. moves the basket, the run number will appear in the correct
The run # and method will be displayed on the first line. station position displayed on the Process Monitor screen.
The message reads, “PLACE THE BASKETS FOR THE 1ST The Process Monitor will be continually displayed while
RUN AT S1 AND PRESS START.” If you wish to change the staining is occurring.
method, press the [METHOD] key and select the
desired method to be run. 2 1
1 2 3 4 5 6 7 8 9 10 11 12 13
NOTE: If a key is not pressed within 30 seconds, the display
E1 E2 16 17 18 19 20 W1W2 W3 24 25 S1 S2 DRY
automatically returns to the Process Monitor screen.
ADD ENDTIME PAUSE REMOVE MAIN
RUN:# 1 METHOD: H&E (SAKURA) START
Process Monitor Screen
PLACE THE BASKETS FOR THE 1ST RUN
AT S1 AND PRESS START
MODE:CONTINUOUS
START METHOD MODE EXIT
2. To add a basket, open the lower door by pressing on

the left side of the door and place the basket set in the
appropriate start station. It is not necessary to open
the cover. Close the door and press the [ST ART] key if
the desired method is already displayed. Pressing
[START] will resume operation and the display will
return to the Process Monitor screen. The process run
number of the added basket set will appear at the
appropriate station position.
Revised 3/30/2010 4.3

ROUTINE OPERATION
2. To obtain a status report of a specific process run, use

Interrupting or Canceling a Staining the arrow keys to highlight that run number and press the
Process [STATUS] key. A screen will be displayed indicating the
run number, staining method name, and the time left to
From the Process Monitor screen, the operator may choose complete the process in hours, minutes and seconds. (If
to interrupt or cancel a staining run. During process the selected staining process has already been finished,
interruption, the robotic arm will not move. The interruption [00:00:00] will be displayed.) Pressing the [EXIT] key will
should be as short as possible to avoid af fecting stain return you to the Process Cancellation screen.
quality if multiple staining programs are in progress.
3. To cancel a staining run, highlight the run number using
To interrupt a staining run: the arrow keys and press the [ABOR T] key from the
Process Cancellation screen. A display message will
1. Press [PAUSE] from the Process Monitor screen. (If the appear, “DISCONTINUE THE FOLLOWING RUN?” With the
robotic arm is moving, the message, “PLEASE WAIT” will selected run number , station number and stain method
appear. When the robotic arm has stopped, the Process identified. Pressing [YES] will cancel the run highlighted.
Cancellation screen will be displayed.) From this screen, Pressing [NO] will return you the previous screen and
the operator has three options, pressing [EXIT] will return [EXIT] will return you to the Process Monitor screen. The
you to the Process Monitor screen and staining will [ABORT] key should only be used if the operator wishes
resume, pressing [ST ATUS] will provide an endtime to discontinue a specific run. The arrow keys, in this
report of a specific run in progress and pressing [ABORT] case, are used to highlight the desired run number which
will cancel a staining run. will be deleted.
5 If a staining run was aborted, the basket set will need to be

1 2 3 4 5 6 7 8 9 10 11 12 13 removed from either the upper or lower level of the
instrument. The displayed message may read, “PLEASE
E1 E2 16 17 18 19 20 W1W2 W3 24 25 S1 S2 DRY OPEN THE DOOR AND THE COVER.” The Process Monitor
ABORT STATUS EXIT screen will automatically appear after the basket has been
Process Cancellation Screen removed for confirmation of removal. V erify that the cursor
highlights the correct station position and press the
[CONFIRM] key. The display will return again to the
Process Cancellation screen or to the Main Menu if there
are no other baskets currently staining.
4.4 Revised 3/30/2010

The “END OF RUN” audible tone is triggered once a run is
Checking the Endtime for a Staining Run completed and has entered either the PE station or one of
in Progress the end stations. The alarm will beep for ten seconds and
While a staining run is in progress, the operator can check continues every 30 seconds until the basket is removed.
the time left for that run and get an estimated time for
completion. By pressing the [ENDTIME] key from the To change the alarm to a single beep, please perform the
process monitor, the screen will display the run number for following steps:
each program currently running and the estimated time
remaining to complete the process in hours, minutes, and 1. If the instrument is not operation turn the power to the
seconds. The staining step, position, and the time left for OFF position.
completion in that specific station is also displayed.
2. Press and hold the “0” key while turning the power ON.
TIME REMAINING TO END
3. Select “1. PROPERTIES”
RUN TIME LEFT STP STA TIME LEFT
1 00:31:28 3 2 00:01:57
2 00:42:27 2 1 00:02:24 4. Select “4. MISCELLANEOUS”
3 00:53:27 1 S1 00:02:46
UPDATE EXIT
5. Select [CONTINUOUS] or [SINGLE] by using the arrow
Endtime Screen keys; confirm by pressing [MEMORY].
NOTE: The clock is not a real-time clock. Y ou may press 6. Turn the power to the OFF position. In the continuous
the [UPDATE] key which will update the times left for each mode, the alarm will beep for 10 seconds in 30 second
run. intervals. The single mode will alarm for 10 seconds and
stop. It is necessary to remove the basket(s) when the
stain process has ended.
End of a Staining Process
When a staining process is completed, the message “THE
STAIN PROCESS COMPLETED. PLEASE REMOVE THE BASKET
FROM E1, E2, OR PE” is displayed and an audible tone is
triggered. The Process Monitor screen will automatically
appear after 15 seconds. The End Station, (E1) and or (E2)
or (PE) is highlighted.
1. Remove the basket set from the (E1), (E2), or (PE) End
Station. You will need to press the [CONFIRM] key to
verify removal of the basket set.
If there are other staining programs in progress, the display

returns to the Process Monitor screen and the stainer
resumes operation of remaining runs. If no other staining
programs are in process, the display will return to the Main
Menu screen.
Revised 3/30/2010 4.5

TROUBLESHOOTING Section 5
General Information
This section helps the operator identify instrument
malfunctions, their probable causes, and corrective actions
that can be taken to remedy the problem. If the malfunction
listed in the troubleshooting section is observed, follow the
instructions given in the REMEDY column. If you have
difficulties in resolving the problem or if the problem is
beyond the scope of this manual, contact Sakura Finetek
for technical support.
The following T roubleshooting Chart lists the warning

messages and error codes that could occur during
operation of the Tissue-Tek® DRS™ 2000 Automatic Slide
Stainer. Possible problems could be electrical, mechanical,
or operational. Probable causes and recommended
remedies are also included so that many isolated problems
can be quickly corrected.
If additional assistance is required concerning an

instrument problem, or if the problem cannot be isolated or
is beyond the scope of this manual, please contact Sakura
Finetek U.S.A. for technical support. Y ou can reach the
Customer/Product Support Department by calling toll free 1-
800-725-8723, option 2 (U.S. only). If located outside the
U.S., contact the nearest T issue-Tek ® distributor or
representative for information and assistance.
Revised 3/30/2010 5.1

TROUBLESHOOTING
TROUBLESHOOTING CHART
PROBLEM POSSIBLE CAUSE REMEDY
If a UPS is connected, the instrument has Power Out Signal The instrument will continue operation for five
received a power failure signal from it. minutes since the UPS supplies back-up power;
however, no heat will be supplied to the drying
station. If the power failure lasts for over five
minutes, the instrument will stop. If the UPS is
connected, wait until the power is restored. If the
UPS is not connected, the [UPS Connect]
parameter needs to be changed to [No] in the
Default Parameter screen.
Display does not light up. Instrument does not Power switch is off. Move the switch to the “I” position.
operate. Power cord is not plugged in. Plug the cord into power source.
Defective power source. Check the laboratory breaker or receptacle.
Display is too dark or too bright. Brightness of display is not properly adjusted. Adjust the display to the desired brightness
level. Use the brightness adjustment dial on the
front, right side of the instrument above the
power switch.
Staining process does not start. (Robotic arm Cover and/or lower door is open or not properly Close the cover and/or the lower door
does not move.) closed. completely.
Error Message [Power Out Signal] is displayed. If the UPS is connected, power failure has
occurred. Staining cannot begin until power is
restored. If the UPS is not connected, the [UPS
Connect] default is set to [YES]. Change the
[UPS Connect] default value to [NO].
Robotic arm suddenly stopped and an audible Robotic arm hit an obstacle. Remove or adjust the obstacle.
alarm sounded. Solution reservoirs are not properly installed. Level solution reservoirs and place them in the
proper position.
Solution or water leaks from the instrument. The drain hose is occluded or bent. Remove any bends or kinks in the hose.
The drain hose is higher than the drain port of Place the hose lower than the drain port, and
the instrument. position it straight.
The drain hose is twisted or looped.
Water supply stainer connection is not properly Tighten the water supply strainer. If solution still
sealed. leaks, the strainer may have a defective gasket.
Contact the Sakura Customer/Product Support
Department.
Wash reservoir does not fill to the proper level. Defective rubber grommet. Replace the grommet. (Product code 4985)
Water flow is not sufficient. The water faucet is closed. Open the water faucet.
The water supply adjustment valve is closed. Open the water supply adjustment valve.
Water flow is not sufficient. Low water supply pressure. Check the water supply pressure, 0.098 to 0.441
MPa (1.0 to 4.5 kgf/cm2) is required for
adequate water supply pressure, (dynamic
pressure).
Clogged screen filter in supply hose. Clean filter screen.
5.2 Revised 3/30/2010

ERROR DESCRIPTION POSSIBLE CAUSE REMEDY
CODE
1 SYSTEM ERROR A control error has occurred. The Turn off instrument power. Call and inform the Sakura
instrument will immediately stop Customer/Product Support Department.
operation.
2 POWER FAILURE The power supply was shut down due When power is restored, the robotic arm will move to its home position and
to a power failure or the power cord the operation will stop. Follow these steps:
was disconnected. 1. (Over station 27) P ower failed for only a short period of time, the
operator can decide to continue the staining process at the point
where the interruption occurred.
2. To continue the process where the instrument was interrupted,
press the [RESUME] key. The staining process will resume.
3. To cancel the process, press the [EXIT] key. To confirm, press
[YES] or [NO], then the “All Basket Removal Confirmation” screen
will appear. Remove all the basket sets from the instrument, and
press the [EXIT] key. The robotic arm will move to the center of the
instrument and the Main Menu screen will be displayed.
3, 5, 6 MEMORY ERROR The instrument memory has been Service is required. Call and inform the Sakura Customer/Product
corrupted. The instrument will Support Department.
immediately stop operation. Any of
Error Codes 3, 5, or 6 will appear in
the upper right hand corner of the
display which relate to the cause of
the memory malfunctions.
4 BATTERY LOW The voltage of the memory back-up is The battery will need to be changed. Call Sakura Customer/Product
low. Support. Although the instrument will continue operation, failure to
replace the battery may induce a memory loss when the instrument is
turned off or if power failure occurs. Pressing the [EXIT] key will allow
operation to resume.
Revised 3/30/2010 5.3

TROUBLESHOOTING
ERROR DESCRIPTION POSSIBLE CAUSE REMEDY

CODE
11-15 ROBOTIC ARM The robotic arm does not operate as The instrument will immediately stop. An error code from Code 11 to
MALFUNCTION expected. 15 will appear in the upper right hand corner of the display according
to which sensor has detected a failure. Follow the steps below:
1. Open the lower door (and the cover, if needed). Look inside the
instrument for an obstacle to the robotic arm movement and
remove it if necessary. Close the cover and lower door and press
the [RESUME] key. After the instrument performs the recovery
operation, the staining process will resume.
2. If the same error message occurs again, press the [EXIT] key. The
“All Basket Removal Confirmation” screen will appear. Remove all
the basket sets from the instrument and press the [EXIT] key. The
robotic arm will move to the center of the instrument and the Main
Menu screen will be displayed.
3. If the error message does not disappear, the driving mechanism or
the position detection mechanism of the robotic arm may possibly
be at fault. Call and inform the Sakura Customer/Product Support
Department.
30 SENSOR OPEN The thermal sensor for the drying The instrument will operate normally except that no heat will be
station does not function. applied to the drying station. Call and inform the Sakura
Customer/Product Support Department.
31 SENSOR SHORT The thermal sensor for the drying Call and inform the Sakura Customer/Product Support Department.
station is short circuited.
32 TEMPERATURE The drying station cannot reach the Call and inform the Sakura Customer/Product Support Department.
LOW set temperature.
33 TEMPERATURE The drying station temperature has Call and inform the Sakura Customer/Product Support Department.
HIGH exceeded the set value.
34 DRY FAN STOPPED The drying station fan has stopped The instrument will operate normally except that no heat will be
operating. applied to the drying station. Repair will be required. Call and inform
the Sakura Customer/Product Support Department.
50 FUME FAN The ventilation fan has stopped. The instrument will continue operation except that the fan will not run
STOPPED Since there is no ventilation inside the instrument, service is required.
Call the Sakura Customer/Product Support Department.
5.4 Revised 3/30/2010

CARE OF THE INSTRUMENT Section 6
5. Release the filter cover by pressing on the black cover

General Maintenance button while holding the filter cover with your left hand,
slide the filter cover slightly to the left and remove it.
Keep the exterior of the DRS 2000 free from dust. Do not
6. Remove the activated carbon filter from the instrument
use solvents of any kind on the front of the control panel;
and dispose of it properly.
however, if disinfection of the keypad is desired, 70% to 85%
ethanol or isopropanol can be wiped on and allowed to air 7. Unpack the new activated carbon filter and reinstall the
dry. The plastic cover may be cleaned with a glass cleaner filter gasket and the new filter.
and soft cloth. 8. Hang the left end of the filter cover on the cover hook of
the instrument and lock the filter cover by pressing on the
black cover button.
Daily Maintenance 9. Return all reservoirs to their original positions, then close
the cover and the lower door.
Exterior/Interior Surfaces
Daily clean the control panel, plastic, and painted areas by
wiping with a clean cloth. Clean the interior of the solution Monthly Maintenance
area by wiping with a clean cloth.
Drying Station
The drying station should be cleaned once a month or more
Bi-monthly Maintenance often as needed.
1. Turn the power of f by pressing the “O” side of the power
Replacement of the Activated Carbon Filter switch. The power switch is located on the front side of the
It is recommended that the activated carbon filter be instrument to the right of the lower door . Open the lower
changed after two weeks or sooner if fume monitoring door first and then the cover. Wait until the temperature of
reveals elevated reagent vapors. the drying station has reached a comfortable level, then
take out the inner lining of the drying station.
Keep the filter in place even if there is an internal duct
system attached. 2. Remove the residue from the inner lining by washing with
water and air drying. Return the inner lining to the inside
To replace the activated carbon filter , follow these of the drying station and verify that the raised portion of
instructions: the liner is facing the right hand side of the drying
1. Turn the power of f by pressing the “O” side of the power compartment.
switch.
2. Open the lower door first and then the cover and remove Solution Reservoirs and Slide Baskets
all the solution reservoirs in the lower section of the The solution reservoirs and slide baskets should be washed
stainer. with tap water or a mild detergent once a month. If you have
NOTE: Label the reservoirs so that you can place them difficulty in removing stains and residue from dried reagents,
back in their original positions. soak first in a commercially available chemical cleaning
detergent or bleach solution and then rinse with a mild
3. Pull each wash reservoir gently toward you to drain any detergent and water . Cleaning reservoirs and slide baskets
water in the reservoir then remove all wash reservoirs. monthly will help keep these items free from permanent
4. Move the robotic arm to the right side of the instrument stains. Always use the same reservoir for a given solution to
with enough care to prevent it hitting on the solution avoid contamination.
reservoirs in the upper section.
Revised 3/30/2010 6.1

CARE OF INSTRUMENT
2. To reattach the strainer , turn it clockwise and tighten it by

Reservoir Trays using your hand only. Fit the drain receiver into the drain
The reservoir trays should be cleaned once a month. T urn port of the reducing valve. At this time, the fastener has to
power off by pressing the “O” side of the power switch. be held in an “open” position. T urn the fastener 90 °
clockwise to lock the drain receiver . Open the water
NOTE: If the robotic arm is interfering with the cleaning
faucet and turn the instrument on.
procedure gently move the robotic arm out of the way , only
if the power is off. CAUTION: Failure to close the external water supply
faucet before attempting to clean the strainer may
Open the lower door first and then the cover . Carefully cause spillage.
remove all the solution reservoirs from the instrument.
NOTE: For a more thorough cleaning, use an ultrasonic
NOTE: Be sure to label the reservoirs in order to put them bath if available. Attach the strainer by turning it clockwise
back in their original position once the cleaning procedure is and tighten it by using your hand only.
complete. NOTE: If there is water a leak at the strainer connection,
Pull each wash reservoir gently toward you to drain out the tighten it using a wrench. If the leak is still present,
water and then remove all solution reservoirs from the lower contact the Sakura Customer/Product Support
section. The lower reservoir tray can be removed by gently Department as the sealing gasket may be damaged.
lifting the front edge with both hands and sliding the tray
away from the front of the instrument. Remove the left and
right reservoir trays from the upper section. Clean the
reservoir trays and the inside of the upper and lower
compartments by wiping with a clean wet cloth. Reinstall the
reservoir trays in the upper and lower compartments. The
lower reservoir tray can be reinstalled by gently placing the
tray under the wash nozzles and sliding the tray toward the
front of the instrument. Return the solution reservoirs and the
wash reservoirs to their original positions. Close the cover
first and then the lower door.
Water Supply Strainer

The water supply strainer should be cleaned once a month.
You will need a toothbrush and a wrench to perform this
procedure.
1. Close the external water supply faucet. Failure to close
the water faucet before attempting to clean the strainer
may cause spillage. Turn the power off by pressing on the
“O” side of the power switch. T urn the fastener 90 °
counterclockwise to release the drain receiver. Pull out the
drain receiver toward you. Using a wrench if necessary ,
remove the strainer by turning it counterclockwise. Use a
cloth to avoid spilling water from the strainer . Wash the
mesh portion of the strainer in water using a toothbrush to
dislodge particles.
6.2 Revised 3/30/2010

SERVICE AND REPLACEMENT PARTS Section 7
Service Information Where to Call for Service

When you have a problem with the If located within the United States, contact the
instrument Customer/Product Support Department of Sakura Finetek
U.S.A., Inc. by calling toll free:
When problems arise during operation of the T issue-Tek®
DRS™ 2000 Automatic Slide Stainer , first refer to Section 5, 1-800-725-8723, option 2
Troubleshooting. A void problems by carefully following
In countries other than the United States, contact the
proper operating and cleaning procedures. If the problem
nearest authorized T issue-Tek® instrument distributor or
cannot be solved and an instrument failure is apparent, our
representative for service information and assistance.
Technical Support Staff is available to assist you.
Before calling for technical support, note the instrument’s
serial number and any error codes. Be sure that you know
the details of the problem you are experiencing. This
information will help the T echnical Specialist to identify the
probable cause of your instrument malfunction.
Revised 3/30/2010 7.1

SERVICE AND REPLACEMENT PARTS
Accessories and
Replacement Parts
Please use the following product codes to order
replacement accessories. Replacement of the battery should
be done by a qualified service technician only
CAUTION: When replacing parts, only use the
recommended parts. The use of non-compatible parts
may cause instrument malfunction.
Accessories
Description Product Code Quantity
Activated Carbon
Filter 2008 2/case
Water Supply Hose 2208 Each
Slide Basket 4768 10/case
Solution Reservoir 4974 Each
Wash Reservoir 4975 Each
Reservoir Lid 4976 Each
Basket Hook 4977 Each
Basket Adapter 4978 Each
Wash Station Plug 4979 Each
Drain Hose 4981 Each
Wash Reservoir
Grommet 4985 Each
Six- Reservoir Lid 4986 Each
Seven- Reservoir Lid 4993 Each
Qualified Service Technician Replacement Parts
Description Product Code Quantity

Battery Unit F51-074-00 Each
7.2 Revised 3/30/2010

STAINING RECORD Appendix A
Sample Staining Record

On the following page is an example page for keeping a
record of your staining programs and progress. It is highly
recommended that you keep records of your individual
programs and notes. Make copies of the blank staining
record for your laboratory use.
The page for recording staining progress is intended only as
a guide; modify it as necessary to satisfy the individual needs
of your laboratory.
Revised 3/30/2010 A.1

A.2 Revised 3/30/2010
Staining Record
Revised 3/30/2010 A.3

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Operating Manual DRS2000 ENG PDF

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Tissue-Tek DRS 2000

Automatic Slide Stainer

© 20 Sakura Finetek USA, Inc.

All Rights Reserved

Revised 3/30/2010 iii

General Description The DRS 2000 consists of four major components:

Revised 3/30/2010 1.1

1.2 Revised 3/30/2010

Fume Control (Figure 1-5)

Revised 3/30/2010 1.3

Leveling Feet/Casters (Figure 1-6)

Power Switch (Figure 1-6, Figure 1-7)

Brightness/Contrast Dial (Figure 1-6, Figure 1-7)

External Connection Terminals (Figure 1-8)

1.4 Revised 3/30/2010

Revised 3/30/2010 1.5

The solution, wash, start, and end stations can be

1.6 Revised 3/30/2010

Europe Solution Names:

Revised 3/30/2010 1.7

3. Carefully lift it into place on a firm, level surface in the

Revised 3/30/2010 2.1

5. The accessory box includes all items necessary for routine

If any of these items are missing, contact your

2.2 Revised 3/30/2010

Drain Elbow Assembly

Revised 3/30/2010 2.3

Activated Carbon Filter Cartridge -1

Water Supply Hose Set -1

Filter Gasket for Carbon Cartridge - 1

Metal Cover for Carbon Cartridge -1

Reagent Trays (for upper level) - 2

Drain Hose Clamp - 1

Reagent Tray (for lower level) - 1

2.4 Revised 3/30/2010

Number of wash Number of

Revised 3/30/2010 2.5

2.6 Revised 3/30/2010

Revised 3/30/2010 2.7

2.8 Revised 3/30/2010

Tissue-Tek DRS 2000

Main Menu Screen

Revised 3/30/2010 3.1

Station Configuration Screen

3.2 Revised 3/30/2010

z If there are already edited staining programs in memory ,

Revised 3/30/2010 3.3

5. The [BACK SPC] key will delete the last character

Deleting a Solution or Staining Method

3.4 Revised 3/30/2010

NOTE: The function key [COUNT 0] will reset the number

Revised 3/30/2010 3.5

3. Once the edit screen is displayed, use the arrow keys to

EXACT MIX + WASH END MORE

Staining Program Editing Screen

3.6 Revised 3/30/2010

Revised 3/30/2010 3.7

3. Move the cursor to the parameter you would like to

2. MIX FREQUENCY 3 times

© 20 Sakura Finetek USA, Inc.