Tissue-Tek TEC 5 ® ™

Tissue Embedding Console System

rating
MODEL NUMBERS
pe
O nual
Ma
5100 (115 VAC, America)
5229 (230 VAC, Europe)
5232 (220-240 VAC, Asia)
5235 (115 VAC, Asia)
5238 (100 VAC, Asia)
 IMPORTANT NOTICE
If this equipment is used in a manner not
specified by the manufacturer, the protection
provided by the equipment may be impaired.

© 2011 Sakura Finetek USA, Inc.

All Rights Reserved

Printed in U.S.A.

Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan
Sakura Finetek Europe B.V., 2804 AV Alphen aan den Rijn, NL

0001338-02 Rev.C
TABLE OF CONTENTS

Section Page
1 INTRODUCTION
General Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1
Operation: Warm Up and Ready Times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.3
2 UNPACKING, SYSTEM DESCRIPTION
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
Carton Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1
System Description
Embedding Module (Front Panel) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.2
Embedding Module (Back Panel) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.3
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.4
Embedding Module (Control Panel) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.5
Cryo Module (Front Panel). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.7
Cryo Module (Back Panel) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.7
Audible Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.7
3 SET UP
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.1
Attach Magnifying Lens Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.2
Verify Proper Installation/System Functioning/Power On. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.3
Access Set Up Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Set Day and Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Set Cryo Module Mode of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Input Cryo Offset Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.4
Change or View Set Temperatures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.5
Add Paraffin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.6
Place Base Molds into Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.6
What Happens When Power Is Turned Off. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.7
When To Set Power Switches On Front Panel To Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.7
4 AUTOMATIC TIMER (OPTIONAL USE)
EMBEDDING MODULE
ON and OFF Times for Selected Days of the Week. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.1
Setting the Automatic Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.1
Checking the Status of the Automatic Timer (What Time is Set?). . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2
5 EMBEDDING PROCEDURE
Embedding Procedure (example) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.1

Revised 07/12/11 iii

TABLE OF CONTENTS
Section Page
6 TROUBLESHOOTING
Error Condition, Cause, Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1
7 CARE OF INSTRUMENT AND MINOR REPAIRS
Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
Routine Maintenance Chart (summary) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1
Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.2
Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.2
Periodic Maintenance (As Required) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.3
Minor Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.6
8 SERVICE AND REPLACEMENT PARTS
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.1
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.3
Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.3
Other Available Equipment and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.4

iv Revised 07/12/11
INTRODUCTION Section 1

GENERAL DESCRIPTION FEATURES

The Tissue-Tek® TEC™ 5 Tissue Q A large insulated work space (allows you to
Embedding Console System is used to manipulate more samples comfortably and effi-
ciently)
embed processed tissue in paraffin, form-
ing paraffin blocks ready for sectioning. Q Individual temperature control in six areas (hot
The System consists of: plate, paraffin chamber, left and right chambers,
Cryo Module, optional electric forceps)
Q Embedding Module
Q Up-front keypad and LCD display (for easy
Q Cryo Module access)

Q Tampers (1 large and 1 small) Q Two 3-hole forceps holders located on each side
of the paraffin dispenser (for right or left hand
Q Transfer Tray operations)
Q Plastic Scraper Q Menu-driven software simplifies setup of the
Automatic Timer (you specify the time you
Q Foot Pedal (sold separately, see Section 8)
require paraffin to be ready. The instrument cal-
culates the time it should automatically turn the
Q Magnifying Lens (sold separately, see Section 8)
heaters on)
Q Electric Forceps (sold separately, see Section 8)
Q Embedding and Cryo Modules work areas of
Q Base Molds (sold separately, see Section 8) the same height (so modules can be placed side
by side, in any order to facilitate your choice of
Q Tissue Cassettes (sold separately, see Section 8) “right-to-left” or “left-to-right” work flow)

Q Large capacity (4L) paraffin reservoir with

optimized paraffin dispensing rates (do more
work, faster)

Q Larger cold spot (accepts larger base molds)

Q Hinged, thermal chamber doors move com-

pletely out of the way (allows full access to both
thermal chambers)

Q Optional magnifying lens (enhances visibility

while positioning tissue specimen)

Q Optional foot pedal (for hand-free operation)

Q Optional electric forceps (always at the right

temperature to allow for more efficient workflow)

Revised 07/12/11 1.1

INTRODUCTION
OPERATION: Cryo Module
WARM UP and READY TIMES The Cryo Module is ready to use (reaches set
temperature) 15 minutes after the compressor is
turned ON. It can also be turned on automatically
Embedding Module
using the automatic timer mode.
When the Embedding Module is turned on, either
manually or automatically, 4 hours* is required for
Temperature can be set down to -10°C in 1°C incre-
paraffin to liquefy and reach set temperature.
ments. The Cryo Module must be connected to the
The Embedding Module stores the instrument set- Embedding Module to operate.
tings in memory whenever power is turned off or is
lost. When power is restored or turned on, a Power NOTE: Make sure the Cryo Module is connected to
Outage message will appear on the display if power the Embedding Module and that the power is on. If
was turned off or lost when the instrument status was the Cryo Module is not powered up or not connected
<READY>, <WARMING UP>, or <CRYO>. A to the Embedding Module, and if the CRYO key on
Power Outage message will not be given if the the Embedding Module key is pressed ON or the
instrument status was <STANDBY>. Automatic Timer software tries to turn the Cryo
Module ON, the following error message will be
Manual Mode: Set power switch on front panels given within 15 seconds:
to ON; press AUTO/MAN key to MAN (green indi-
cator illuminates). Five areas begin to heat when the <CRYO ERROR> CRYO POWER IS OFF OR
HEAT key is pressed ON. All areas, except the COOLING FAN ERROR
paraffin chamber and the left and right chambers, To silence the alarm press any key.
reach set temperature within 60 minutes. The paraf-
fin and the left and right chambers reach set temper- To operate the Cryo Module if this situation occurs,
ature in 4 hours.* Using paraffin already in the first turn the Embedding Module power switch to
molten state will significantly reduce the warming OFF, verify that the Cryo Module is connected to the
up time. The instrument status will be <WARMING Embedding Module, and turn the Cryo Module
UP>. When all heated areas reach set temperature, power switch to ON. Then turn the Embedding
the instrument status will change to <READY>. Module ON again.
The heat remains ON until you manually press the
HEAT key again. NOTE: The Cryo Module’s power indicator will
Automatic Timer Mode: The Embedding Module stay on when the Cryo Module is turned off using
can be preset to be ready to use at your specified the Cryo key on the Embedding Module.
time. The instrument calculates an automatic “turn
on” time from your preset ready time. When the Ensure your safety! Comply with all
instrument automatically turns ON, only the paraffin WARNING and CAUTION statements found
chamber begins to heat. The remaining four areas in this manual.
begin to heat 60 minutes prior to the preset ready
time. During that time, the instrument status will be
<WARMING UP>. When all set temperatures are
reached, the instrument status will change to
<READY>. The module automatically turns OFF at
your preset “Shut down” time or if you select the
MANUAL mode and press HEAT.

*May be longer depending on current source and environmental

factors.

1.2 Revised 07/12/11

SPECIFICATIONS
EMBEDDING CRYO
MODULE MODULE

Ambient Operating 10° – 40° C 10° – 40° C

Temperature and
Relative Humidity Range 30% – 85% RH, 30% – 85% RH
(noncondensing) (noncondensing)
Dimensions/Weight Width 57.5 cm (22.6 in) Width 33.0 cm (13.0 in)
Depth 64.2 cm (25.3 in) Depth 61.7 cm (24.3 in)
Height 37.7 cm (14.8 in) Height 37.7 cm (14.8 in)

Weight approx. 26 kgs (57 lbs) Weight approx. 22 kgs (48 lbs)
Power Required Model No. 5238: 100 VAC ±10% or less,
100 VAC ±10% or less, @ 2.0 amps 50/60 Hz
@ 9.0 amps 50/60 Hz
Model Nos. 5100, 5235: 115 VAC ±10% or less,
115 VAC ±10% or less, @ 2.0 amps 60 Hz
@ 9.0 amps 60 Hz
Model Nos. 5229, 5232: 220 – 240 VAC ±10% or
220 – 240 VAC ±10% or less, @ 2.0 amps 50 Hz
less, @ 9.0 amps 50 Hz
Chamber Capacity Paraffin Chamber:
approx. 4 liters (no chambers)
Left and Right Chambers:
approx. 1.5 liters
Temperature Range Paraffin Chamber, Right and Cooling Plate:
Available Left Chambers, Hot Plate: –10°C to 0°C
50°C – 75°C
Electric Forceps(optional):1 to 5
Factory Set Temperature Paraffin Chamber, Left and Cooling Plate:
Right Chambers, Hot Plate: – 5°C
62°C
Cold Spot: approx. 15°C at
25°C room temp. and
40% R.H.
Electric Forceps(optional): 3
Circuit Breaker/ON-OFF 15 amps
Switch
Memory Backup Long-life battery
Safety Standards Tested and listed by Intertek Testing Services (ITS)
Complies with: UL 3101-1, 1st ed., IEC 1010-1 and
CAN/CSA-C22.2 No. 1010.1-92

Revised 07/12/11 1.3

UNPACKING, Section 2
SYSTEM DESCRIPTION
UNPACKING Q Use extreme care when unpacking each module.
Each module contains sensitive electronic parts.
The Tissue-Tek® TEC™ 5 Tissue Embedding Check each item for shipping damage.
Console System arrives in two cartons tied together. Q If there is visible shipping damage, immediately
file a complaint with the carrier. Then, notify your
Inspect each carton for shipping damage. nearest Tissue-Tek instrument distributor.
Unpack each carton.



Missing items?…Dial 1-800-725-8723 (U.S.

CARTON CONTENTS Customers, only). If located outside the U.S.,
contact your nearest Tissue-Tek instrument

Embedding Module distributor.
 Cryo Module
 Cryo Module Power Cord  
 Transfer Tray
 Tampers (1 large and 1 small) 
 Plastic Scraper
 Connecting Cable to Embedding Module
 Embedding Module Power Cord
Operating Manual (not shown – instructions for
both Embedding and Cryo Modules) 

 

Revised 07/12/11 2.1

UNPACKING,
SYSTEM DESCRIPTION

  

SYSTEM DESCRIPTION

Embedding Module
(FRONT PANEL)  Forceps Warmer Holders – hold forceps; can
be set to any temperature at or between

Paraffin Chamber – holds up to four liters of 50°–75°C (122°–167°C) using the Hot Plate
molten paraffin; fine mesh filter at bottom of temperature settings; factory set at 62°C (149°F).
chamber catches debris; can be set to any tem-
perature at or between 50°–75°C (122°–167°F); Paraffin Dispenser – molten paraffin is dis-
factory set at 62°C (143.6°F). pensed here; press fingerplate to dispense paraf-
fin or use the optional foot pedal; turn knob
 Power Switch – turns the power to the module “clockwise” to decrease flow or “counterclock-
ON or OFF; circuit breaker. wise” to increase flow. Optional foot pedal (not
shown) can be used instead of the fingerplate to
 Control Panel – consists of an LCD display and dispense paraffin.
keypads (each component discussed in detail
later in this section).  Work Light – increases visibility in hot plate
area making tissue positioning easier.
 (Optional) Electric Forceps – (Connector
shown only) connects underneath the control  (Optional) Magnifying Lens Assembly –
panel. Adjustable to 5 different temperature lev- increases visibility while positioning tissue.
els.

2.2 Revised 07/12/11

 Left and Right Chambers – can be set to any
temperature at or between 50° – 75°C
(122°–167°F); each chamber can be used for one

of the following at any one time (preference
depends on your chosen work flow direction):

Q warming area for various sized base molds (close

lid to keep heat in and dust out)
OR
Q paraffin bath to keep processed tissue specimens at
the appropriate temperature (close lid to keep heat
in, and dust out, when embedding process is fin-
ished or temporarily stopped).


Transfer Tray – may be used to transfer
processed specimens to the Embedding Module
or may be half-filled with paraffin and then used
as a paraffin bath.

 Waste Drawers – catch molten paraffin that drains

through two holes in grooves around hot plate
area. Embedding Module
(BACK PANEL)
 Cold Spot – cools liquid paraffin at the bottom
of a base mold so tissue can be correctly posi-
Cryo Module Connector – accepts the plug to
tioned. the Cryo Module. The Cryo Module operation is
controlled by the Embedding Module.
 Hot Plate – ensures that paraffin does not solidi-
fy during embedding process; can be set to any  Power Cord Connector – accepts the plug for
temperature at or between 50°– 75°C connecting the power cord to the module.
(122°–167°F); factory set at 62°C (143°F). This
temperature setting is also used for the Forceps
Warmer Holders.

 (Optional) Foot Pedal – for hands-free paraffin

dispensing.

 Display Adjustment Wheel – controls the con-

trast for the display.

Revised 07/12/11 2.3

UNPACKING,
SYSTEM DESCRIPTION
Accessories
(found inside paraffin chamber; refer to photo on
page 2.1)

Embedding Module Power Cord – carries electri-

cal current to Embedding Module

Tampers (1 large and 1 small) – used (as neces-

sary) to press tissue firmly against the bottom of the
base mold

Plastic Scraper – used to remove frost from cooling

plate of Cryo Module

2.4 Revised 07/12/11

 




Embedding Module  AUTO/MAN key – selects between automatic

(CONTROL PANEL) and manual operation. The corresponding LED
will be lit to indicate the current mode of opera-

LCD Display – consist of two lines of up tion. Selecting the Manual Mode of operation
to 40 characters per line. does not permanently cancel the automatic timer
On/Off times.
Displays (depending on the mode):
(a) current time of day  LIGHT key– turns Work Light ON or OFF.
(b) On or Off times
(c) current temperatures  CRYO key – turns the Cryo Module ON or OFF.
(d) actual preset temperatures The independent operation of the CRYO Module
(e) actual operating mode can be restricted if the Automatic Timer has been
(f) system status information and error condi- programmed to also control the ON and OFF
tions times of the Cryo Module. Under this condition,
the CRYO key will not operate when the system
 Left and right arrow keys– move the display operating mode is in AUTO (AUTO LED indica-
cursor left and right. Rapid scrolling is enabled tor is ON). See Section 3, SET UP to change the
by holding these keys for more than 2 seconds. setting. NOTE: Power to the Cryo Module
must be turned on before the Embedding Module
is turned ON.

Revised 07/12/11 2.5

UNPACKING,
SYSTEM DESCRIPTION
 HEAT Key – turns the heating components of
the Embedding Module ON or OFF.

 SET key – allows operator to enter the SETUP

mode (See Section 3, Set Up).

 EXIT key – allows to return to the previous

menu item without saving the changes made in
the SETUP mode.

 + and – keys – increase or decrease numerical

values and add or delete days when in the
SETUP mode. Holding these keys for more than
2 seconds enables rapid scrolling.

ENTER key – when pressed, stores data into

memory in the SETUP mode.

 Display Adjustment Wheel – controls the con-

trast for the display.

2.6 Revised 07/12/11

 

Revised 07/12/11 2.7

SET UP Section 3

INSTALLATION 5. Connect the Cryo Module and Embedding

Module power cords into properly grounded out-
CAUTION: Do not place the Tissue-Tek® TEC™ 5 lets. The power cord with the right angle con-
Embedding System where it would be subjected nector plugs into the Cryo Module.
to direct sunlight and/or direct heat, an open
flame source, excessive humidity or air current, 6. Connect the Foot Pedal (sold seperately, refer to
excessive particulate matter, or electrical noise section 8) to the back of the Embedding Module.
(such as from refrigerators, centrifuges, ultra-
sonic cleaners and microwave ovens). 7. Move the modules into place on the designated
work space.
1. Select a level surface near one properly ground-
ed electrical outlet in a well-ventilated area of CAUTION: For cooling purposes, allow at
minimum humidity. least 76 mm (3 in) of space between the wall
(or other surface) and the back panel of each
CAUTION: Make sure the electrical outlet module.
supplies the proper voltage (within ± 10%).
Refer to the rating label on the Embedding
Module.

2. Place the Embedding Module next to the Cryo

Module. It is not necessary to leave any space
between the two modules.

NOTE: The working area of each module is of

the same height. This allows you to place the
modules next to each other, in any order, to
accommodate your choice of “right-to-left” or
“left-to-right” work flow.

3. Check to make sure the power switches on the

front panel of the Embedding Module and the
Cryo Module are set to OFF (O).

4. Connect the Cryo Module to the Embedding

Module using the provided cable. The end of
this cable having the right-angle connector is
plugged into the Cryo Module. The end of this
cable having the straight connector is plugged
into the Embedding Module.

Revised 07/12/11 3.1

SET UP
ATTACH MAGNIFYING LENS CONNECT ELECTRIC FORCEPS
ASSEMBLY (sold separately, refer to section 8)
(sold separately, refer to Section 8)

1. Remove the screw from the top of the 1. Connect the wire underneath the Embedding
paraffin dispenser. Module control panel.

2. Mount assembly in the threaded hole of the

paraffin dispenser. The end of the flexible attach-
ment screws directly into the hole. Tighten.
3. Adjust magnifying lens to preferred focal range
(pull lens forward and down).

3.2 Revised 07/12/11

VERIFY PROPER INSTALLATION If System functions properly:
AND SYSTEM FUNCTION
Q press CRYO key to turn off compressor (the mes-
1. Set the power switch on the front panel of the sage CRYO IS OFF is back on the display);
Cryo Module to ON (I).
Q continue with “Set Day and Time ” procedure
2. Set the power switch on the front panel of the (next page).
Embedding Module to ON(I).
NOTE: Paraffin will not be dispensed and an audi-
The MAN indicator is illuminated.
ble alarm will sound if the fingerplate or the optional
foot pedal is pressed unless <READY> is displayed
The following appears on the LCD display:
on the top left end corner.
(The time and day may be different)

<STAND BY> 6:30PM WED

If System does not function properly:

If the LCD display appears dark (no characters Dial 1-800-725-8723 (U.S. Customers, only). If
visible), rotate the display adjustment wheel fully located outside the U.S., contact your nearest Tissue-
in both directions until the display characters are Tek instrument distributor.
easily read.

3. Press HEAT key (on Embedding Module control

panel). The following appears on the LCD dis-
play:

<WARMING UP> CRYO IS OFF 6:30PM WED

PLEASE WAIT.

4. Press CRYO key (on Embedding Module control

panel).

The “CRYO IS OFF” message will disappear

from the display and the compressor will start.

Revised 07/12/11 3.3

SET UP
ACCESS SET UP MODE SET CRYO MODULE MODE OF
OPERATION
Access the SET UP mode by pressing the SET key.
The first two menu items are displayed. The Cryo Module can be operated independently of
the Embedding Module when the MAN/AUTO keys
SETUP MODE> SELECT MENU (1-7) is set to MANUAL (MAN LED is illuminated). The
1. TEMPERATURE 2. CRYO MODULE CRYO key can then be used to turn the Cryo Module
ON or OFF.
The other five menu items are:
NOTE: The Cryo Module power switch must be
3. TIMER 4. CLOCK turned ON first. The Embedding Module power
switch must then be ON to operate the Cryo module.
5. TIME FORMAT 6. ERROR LIST
When AUTOMATIC operation is selected (AUTO
7. CRYO OFFSET LED is illuminated) control of the Cryo Module ON
and OFF times can be synchronized with the ON and
Pressing the ENTER key will allow access to the OFF times of the Embedding Module or can be inde-
item number highlighted by the cursor. pendent. To synchronize the operation of the Cryo
Module with the Embedding Module, enter the
SET DAY AND TIME SETUP mode by pressing, the SET key and high-
light “2. CRYO MODULE”. Press ENTER and
Q Clock settings are not lost when a power failure select item “1. TIMER ON” using the Arrow keys
occurs or when the Embedding Module power and pressing ENTER. If independent operation of
switch (on the front panel) is set to OFF. The the Cryo Module is preferred, select “2. TIMER
instrument memory is backed-up by a battery. At OFF”. If the Timer OFF option is selected, the
installation and periodically as required, it may be CRYO key must be pressed to turn the Cryo Module
necessary to set the clock. on.

To select a 12-hour or 24-hour time format, use the

arrow keys to move the cursor to item “5. TIME
FORMAT” and press ENTER. Then, use the arrow INPUT CRYO OFFSET VALUE
keys to select the desired format and press ENTER.
Since the Embedding Module computer controls the
When ready to set the actual day and time, use the operation of the Cryo Module, an offset value must
arrow keys to move the cursor to item “4. CLOCK” be entered to ensure temperature accuracy.
and press ENTER. Use the Arrow keys to highlight
the actual day of the week and press ENTER. A Write down the offset value found on the Cryo
prompt will then appear to input the current time. Offset Label located at the back of the Cryo Module.
Use the Arrow keys to move the cursor between The range is -4.9 to +4.9.
“Hour” and “Minutes”. Use the + and - keys to set
the actual values. When finished, press ENTER to Enter the SETUP mode by pressing the SET key and
save the values into memory. If you do not want to highlight “7. CRYO OFFSET”. Press ENTER and
save the changes, press the EXIT key instead. use the + and - keys to set the actual value. When
finished, press ENTER to save the value into memo-
ry. If you do not want to save the changes, press the
EXIT key instead.

3.4 Revised 07/12/11

SET UP
CHANGE OR VIEW SET *Factory set temperatures:
Paraffin Chamber 62°C (143.6°F)
TEMPERATURES Hot Plate 62°C (143.6°F)
Left and Right Chambers 62°C (143.6°F)
Q Temperature settings are retained in memory
Cryo Module -5°C (23.0°F)
and are not lost when a power failure occurs or
Electric Forceps (optional) 3
when the Embedding Module power switch
(on the front panel) is set to OFF.
It is recommended that factory set chamber temperatures be veri-
Q During operation, actual temperatures will be dis- fied prior to first use of the System.
played and the Arrow keys can be used to scroll
through all six temperature controlled compo-
nents. If the AUTO MODE is on, the
SHUTDOWN time will also appear under item 7.

To change or view the set temperatures*, access the

SETUP mode by pressing the SET key. Highlight
“1. TEMPERATURE” and press ENTER. The fol-
lowing information will be displayed:

<SET TEMPERATURES> SET UP (1-6) (50°C-75°C)

1. PARAFFIN: **°C 2. CRYO : **°C

Use the Arrow keys to select the desired component

and use the + and - keys to set the temperatures.
When finished, press ENTER to save the values into
memory or EXIT to discard the changes.
Temperature can be set for the following compo-
nents in 1°C increments:

1. Paraffin Paraffin Chamber from 50°C to 75°C

2. Cryo Cryo Module from -10°C to 0°C
3. Hot Plate Hot Plate from 50°C to 75°C (The Hot
Plate setting also controls the Forceps
Warmer Holders)
4. Left Left Chamber from 50°C to 75°C
5. Right Right Chamber from 50°C to 75°C
6. Forceps Optional Electric Forceps from 1 to 5

Revised 07/12/11 3.5

SET UP
ADD PARAFFIN PLACE BASE MOLDS INTO
(Embedding Module) CHAMBER
Do not use paraffin with a melting point Place base molds (sold separately, see Section 8) into
higher than 75°C (167°F).* empty chamber (either right or left depending on
your chosen work flow direction).
*or paraffin will not liquefy; the maximum temperature of
the paraffin chamber is 75 ± 2°C (167.0 ± 3.6°F)

WARNING: MOLTEN PARAFFIN IS HOT AND

MAY CAUSE BURNS. DO NOT OVERFILL
PARAFFIN CONTAINER.

1. Open Paraffin Chamber Lid.

2. Add approximately 4 liters of clean molten paraf-

fin (or equivalent volume of paraffin chips/flakes)
to the chamber. Do not overfill.

NOTE: 2 parts dry flakes/chips = 1 part molten paraffin

3. If using the transfer tray as the paraffin bath,

add equivalent of 50 mL of molten paraffin** to
either right or left chamber (whichever you select
depending on your work flow direction)

**added to increase heat conductivity between tray and

chamber

Place transfer tray into the chamber (over 50 mL

molten paraffin).

4. Add paraffin to the paraffin bath to a depth of

about 13 mm (0.5 in), or about .9 liter (.24 gal)
molten paraffin or equivalent in dry chips/flakes.

4. NOTE: The paraffin bath may be either the right or left

chamber or transfer tray (whichever you select).

4. NOTE: Change paraffin frequently to prevent build-up

of tissue debris.

5. Pour about 1 mL of clean molten paraffin into

each well in forceps holder.

6. Place one forceps into each well.

3.6 Revised 07/12/11

WHAT HAPPENS WHEN POWER WHEN TO SET POWER SWITCH
IS TURNED OFF ON FRONT PANEL TO OFF
Embedding Module Set power switch to OFF when modules are not in
When the power is turned off for maintenance proce- use for extended periods of time (e.g., over a month),
dures, etc. (via power switch on front panel) or and when performing designated maintenance proce-
interrupted by a power failure: dures.

Q current time of day and all instrument settings are

not lost

Q preset On/Off times will be retained and will

occur as scheduled

Cryo Module
To protect the compressor from damage, the com-
pressor will not restart for 3 to 5 minutes if power
is interrupted or turned off while the compressor is
ON. The fan will start immediately.

When the Cryo Module is intermittently not in use

during the day, turn the compressor OFF by pressing
the CRYO key on the Embedding Module control
panel. This action will not be allowed if the Cryo
Module operation was set to synchronize with the
Embedding Module and the mode of operation
selected is AUTO (AUTO LED is illuminated).

Revised 07/12/11 3.7

AUTOMATIC TIMER (optional use) Section 4

ON AND OFF TIMES FOR Days already programmed will be annoted by a “>”
SELECTED DAYS OF THE WEEK: symbol. The current day of the week will be high-
lighted. In the above example, the Automatic Timer
Q can be preset* will turn on and off the TEC 5 from Monday to
Q can vary daily (for up to 7 days) Friday but not during the weekends; the current day
Q is automatically reused from week to week is Tuesday.
Q can be overridden at any time using the
MAN/AUTO key Use the Arrow keys to select a day of the week.
Q can be easily cancelled Press the + key to add to the list of days where auto-
Q can be used to also turn the Cryo Module on and matic operation is performed. An asterisk will
off automatically appear, indicating that day has been selected. To
remove a day from the list, move the cursor to high-
*The System calculates an automatic “ ON” time from your preset
“ready” time. The calculation is based on normal room tempera- light it and press the – key.
ture conditions. “Ready” time may be delayed as much as 1 to 1½
hours, if room temperature approaches the low end of the operating
temperature specification. Add to the list all days that will be controlled by the
Automatic Timer. When done, press ENTER to save
the list into memory. Days can be set individually or
SETTING THE AUTOMATIC as a group. The display will then prompt you to
TIMER input the ON and OFF times.
Q Automatic Timer settings are stored in memory
The following example uses the 12 hour clock format:
and are not lost if a power failure occurs or when
the power switch on the front panel is set to OFF. <SET TIMER> INPUT ON AND OFF TIMES
Q To control the Cryo Module ON/OFF times the 10:00 AM [ON] – 5:00 PM [OFF]
Cryo Module Operation must be set as “TIMER
ON”. (See page 3.4, set Cryo Module Mode of Use the Arrow keys to move between the fields and
operations). use the + and – keys to set the desired times. When
finished, press the ENTER key to save the values
IMPORTANT--In the following instruc- into memory or press EXIT if you do not want to
save the changes.
tions: “ON” time refers to the time
you want the modules to be “ready to
use” and “OFF” time refers to the
time you want to turn them off.

To program the Automatic Timer, access the SET

UP mode by pressing the SET key and highlight
menu item “3. TIMER.” Press ENTER. The fol-
lowing information will be displayed:
<SET TIMER> SELECT DAYS OF OPERATION.
>MON >TUE >WED >THU >FRI SAT SUN

Revised 07/12/11 4.1

AUTOMATIC TIMER (optional use)
CHECKING THE STATUS OF THE
AUTOMATIC TIMER
(WHAT TIME IS SET?)
To view these times whatever the current operating
mode is, press the SET key and use the arrow keys
to move the cursor to item “3. TIMER”. Press the
ENTER key. Then, highlight any day annotated
with a “>” and press the + key and then ENTER.
The ON and OFF times will be displayed. Use the
EXIT key to return to any previous menu.

If the operating mode is AUTO (AUTO LED is illu-

minated), and the system is in Standby, the following
information will be displayed.

<STANDBY> 6:00 PM WED

NEXT ON/OFF CYCLE: 8:00AM –5:00PM TUE

When the instrument is in STANDBY, all heaters are

off and the Cryo module is off. To turn the heaters
on before the next scheduled ON cycle, press the
HEAT Key. To turn the Cryo Module ON, press the
AUTO/MAN key to illuminate the MAN LED, then
press the CRYO key (NOTE: The CRYO key can
be pressed to turn the CRYO Module ON even if the
AUTO LED is illuminated if the “2. TIMER OFF”
item was programmed in the SETUP Mode).

4.2 Revised 07/12/11

EMBEDDING PROCEDURE Section 5

EMBEDDING PROCEDURE 4. Select (and remove from heated chamber) one

(Example) base mold that would best fit the tissue(s) in the
cassette. Place under the paraffin dispenser.
NOTE: The following procedure is an example
only. You may prefer to use your own method. 5. While holding base mold under paraffin dis-
penser, press fingerplate or use the optional
pedal to dispense only enough paraffin to half-
Before proceeding, make sure: fill base mold.
Q both modules are properly set up according to
6. Place base mold on hot plate under paraffin dis-
instructions in Section 3 (and Section 4, if auto-
penser.
matic timer is used)

Q both modules are ready to use (check temperature 7. Using forceps, place tissue(s) from cassette into
indicators, paraffin volume, base molds, paraffin base mold.
tray)
If tissues do not sink to bottom of mold, use
Q tissues have been processed and are in the paraffin tampers or forceps to lightly push tissue(s)
bath of the Embedding Module. down (into proper position) on the bottom of
base mold.

8. Move the base mold to the cold spot. Bottom of

Embedding Procedure the base mold rapidly cools and a thin layer of
(example) paraffin solidifies.

NOTE: If the tissue is not correctly positioned,

1. Press LIGHT key to turn on Work Light. move base mold back to hot plate; then use for-
Optional: ceps to reposition tissue(s). Return base mold
Properly position magnifying glass for best to cold spot. Repeat this procedure as necessary
view until tissue(s) are properly oriented.

2. Using heated forceps, remove one cassette NOTE: Never allow paraffin to completely
from the paraffin bath and place on either solidify during this process.
right or left hot plate (depending on your
work flow direction). 9. Place one cassette over properly positioned tis-
sue(s) in base mold.
3. Remove cassette cover, if required.
Embedded tissue(s) adhere to cassette.

Revised 07/12/11 5.1

EMBEDDING PROCEDURE
10. Move the base mold to the hot plate under
paraffin dispenser.

11. While holding the cassette under paraffin dis-

penser (and above center hot plate), press fin-
gerplate or use optional foot pedal to dispense
paraffin into base mold until the cassette is
filled with paraffin.

NOTE: Do not overfill base mold.

12. Place base mold containing embedded tissue(s)

onto cooling plate.

NOTE: The surface of the cooling plate will

usually be covered with a light layer of frost
(depending on ambient humidity). This
enhances heat exchange between the warmed
base mold and the cooling plate.

13. Check to make sure the paraffin block is com-

pletely solidified; then remove paraffin block
from base mold.

NOTE: The paraffin block should easily release

from the base mold. If not, the base mold may
need cleaning. Use of the Tissue-Tek®Mold
Release Concentrate (product code 4141) will
make demolding easier.

14. If the paraffin block is not to be sectioned

immediately, the block may be stored on the
cooling plate.

15. Press LIGHT key to turn off Work Light.

NOTE: To extend life of light bulbs, always

turn off Work Light after embedding procedure
is completed.

5.2 Revised 07/12/11

TROUBLESHOOTING Section 6

Error conditions are listed in alphabetical order.

Use the table below to quickly determine if this section addresses

your particular instrument problem.

Cryo Module Page

Alarm sounds................................... 6.2
Cooling plate does not function............... 6.2
Cooling plate does not reach
set temperature .............................. 6.2
Power indicator will not illuminate........... 6.2

Embedding Module Page

Alarm sounds and error message is
displayed ..................................... 6.3
Paraffin will not dispense correctly........... 6.4
Power indicator/other indicators will
not illuminate ................................ 6.4

In the event this section cannot assist you in solving your

instrument problem, call:

1-800-725-8723
(U.S. Customers, only)

If located outside the U.S., contact your nearest Tissue-Tek®

instrument distributor or representative.

Revised 07/12/11 6.1

TROUBLESHOOTING
CRYO MODULE
ERROR
CONDITION CAUSE SOLUTION

Alarm (tone) sounds for Cryo Module has Turn Embedding Module power to OFF. Verify that
10 seconds or error malfunctioned or Cryo the Cryo Module is connected to the Embedding .
message appears on the Module power is OFF or Module. Turn the Cryo Module power ON. Turn the
Embedding Module is not connected to the Embedding Module power ON.
display Embedding Module If the error persists, press “SET” key. Highlight
menu item 6. ERROR LIST and press ENTER.
Record the error indication and press EXIT.
Contact Customer Support.

Cooling plate does not Power outage occurred Check to make sure:
function or compressor has Q the power cord is connected to a “live” outlet
malfunctioned. Q the connecting cable is securely attached to the

Q Cryo and the Embedding Modules.

Q the power switch on the front panel of the

Embedding Module is set to ON and power indica-

tor is illuminated
Q the CRYO key on the Cryo Module control

Q panel is ON

If the problem persists, contact Customer Support.

Cooling plate does not Cryo Module has Check to make sure:
reach set temperature malfunctioned. Q at least 76 mm (3 inches) of space exists between

Q the wall and module

Q the module is not in an area of excessive air

Q current, direct heat or sunlight, excessive humidity,

Q excessive particulate matter, or electrical noise

If the problem persists, contact Customer Support.

6.2 Revised 07/12/11

EMBEDDING
MODULE ERROR
CONDITION CAUSE SOLUTION

Power indicator will not Power outage occurred Check to make sure:
illuminate or Cryo Module has Q power cord is securely plugged into a “live”
malfunctioned. Q power outlet.

Q the power switch on the front panel of Cryo

Module is set to ON.

Q The cable connecting both modules is firmly

inserted at both ends.

If the problem persists, contact Customer Support.

Alarm (tone) sounds for Temperature is Press “SET” key. Highlight menu item
10 seconds; out-of-range by more “6. ERROR LIST” and press ENTER. Record
than 5°C (9°F) the error indication and press EXIT.
If more than one error code is shown, use the
arrow keys to move the cursor to highlight the next
error code number, and record the error indication.

Embedding Module Check to make sure:

status never reaches Q if you have recently added cold paraffin, base

<READY> Q molds, install a cool forceps warmer, etc.,

Q that may have caused more than a 5°C drop

Q in an area temperature. If so, wait a few minutes

Q for the temperature to stabilize and the error

Q condition will then cease.

Q that at least 76 mm (3 inches) of space exists

Q between the wall or other surface and the

Q back panel of the module.

Q that you have checked the temperature of the

Q room. Adjust the temperature if necessary

Q the AUTO/MAN key is set to MANUAL and

the HEAT key is pressed ON.

Q The AUTO/MAN key is set to AUTO and the

ON and OFF times for the current day are set

properly.

If the error condition persists, contact Customer

Support.

Revised 07/12/11 6.3

TROUBLESHOOTING

EMBEDDING
MODULE ERROR
CONDITION CAUSE SOLUTION

Paraffin will not Paraffin may not be Q Check that the LCD display shows <READY>
dispense molten. Q Check the temperature of the room; adjust
temperature if necessary.
Q If using the automatic timer, check to verify
that the correct AM or PM “On” time is set.
Q If using the manual mode, wait at least 4*
hours after the module is turned ON for
paraffin to liquefy and reach set temperature.
Q Check the temperature of the paraffin
chamber. If the temperature is slightly below
the set temperature and decreases over a long
time period, the heater has failed. Contact
Customer Support.

*May take longer depending on current source and

environmental factors.

Paraffin filter may If paraffin is molten and set temperature has been
need cleaning. reached, paraffin flow may be obstructed by a
clogged paraffin filter. Refer to Section 7 for
cleaning instructions.

If the problem persists, contact Customer Support.

Power indicator/other Power outage or Check to make sure:

indicators will not Embedding Module Q power cord is securely connected to the

illuminate has malfunctioned. Q module and plugged into a “live” power outlet.

Q the power switch on the front panel is set to ON.

If the problem persists, contact Customer

Support.

LCD display is dark Display adjustment Rotate the display adjustment wheel fully in both
wheel misadjusted. directions until the display characters are easily
read.

6.4 Revised 07/12/11

CARE OF THE INSTRUMENT Section 7
AND MINOR REPAIRS
INSTRUMENT CARE Q Set the power switch on the front panel of both
Modules to OFF when modules are not in use for
The Tissue-Tek® TEC™ 5 Embedding and Cryo long periods of time.
Modules contain sensitive electronic parts.
Q During the day, when the Cryo Module is not
Q Handle modules with extreme care. Severe being used, turn OFF the compressor (use CRYO
mechanical shock can damage and/or dislodge key on control panel).
internal parts and connections.
Q Do not place anything on the top panels of either
Q Do not place the system where it would be sub- module. Placing items on the top panels interferes
jected to direct sunlight and/or heat, an open with proper ventilation and heat exchange.
flame source, excessive air currents, excessive
humidity, excessive particulate matter, or electri- Q WARNING: MOLTEN PARAFFIN MAY
cal noise (such as from refrigerators, centrifuges, CAUSE BURNS. DO NOT MOVE THE
ultrasonic cleaners, and microwave ovens). EMBEDDING MODULE WHEN THE
PARAFFIN CHAMBER (OR PARAFFIN
Q Make sure the cable connecting both instruments BATH) IS FILLED WITH MOLTEN
together is properly inserted at both ends and that PARAFFIN. TURN THE POWER OFF,
the cable is not crimped. THEN ALLOW THE PARAFFIN TO SOLID-
IFY BEFORE MOVING THE MODULE. DO
Q Do not block the ventilation panels on the back NOT REMOVE THE TRANSFER TRAY IF
panel of each module. Allow at least 76 mm (3 in) IT IS FILLED WITH MOLTEN PARAFFIN.
of space between the wall (or other surface) and ALLOW PARAFFIN TO SOLIDIFY
back panels. BEFORE REMOVING TRAY.

ROUTINE MAINTENANCE CHART (Summary)

Periodic and As Required (by spillage,
Daily troubleshooting or contamination)

Replenish Paraffin (Embedding Module) Clean Paraffin Bath, Paraffin Chamber and
Filter, Forceps Wells, Exterior Surfaces and
Remove Spilled Paraffin/Debris from Ventilation Panels, Waste Drawer, and
insulated workspace and hot plate area Chamber storing Base Molds; add clean
(Embedding Module) paraffin (Embedding Module)

Turn off compressor (press CRYO key on Clean Magnifying Lens Assembly (optional
Embedding Module Control Panel) purchase for Embedding Module)

Remove condensate from cooling plate (Cryo Clean exterior surfaces (Cryo Module)
Module)

Revised 07/12/11 7.1

CARE OF THE INSTRUMENT

DAILY MAINTENANCE
Embedding Module Cryo Module
‹ REPLENISH PARAFFIN Turn off compressor (press CRYO key on
Embedding Module Control Panel).
WARNING: MOLTEN PARAFFIN MAY
CAUSE BURNS. DO NOT OVERFILL Allow cooling plate to reach ambient temperature.
PARAFFIN CONTAINER.
Remove condensate from cooling plate (use an
Q 2 parts dry paraffin = 1 part molten paraffin. absorbent cloth/towel).

Q The paraffin chamber holds 4 liters (1.06 gal)

of molten paraffin.

Q The paraffin bath (either transfer tray or

chamber, whichever you are using) should be
half-filled or about .9 liter (.24 gal) with DISINFECTION
paraffin.
After performing daily maintenance, you may disin-
NOTE: Refer to Section 3 for complete instructions
regarding “Adding Paraffin.”
fect the surfaces and keypads (if desired) as follows:

Q Wipe area using a soft cloth dampened in alcohol

(70 – 75% solution isopropyl or ethyl).
‹ REMOVE SPILLED PARAFFIN/DEBRIS
Q Do not wipe dry. Allow area to air dry to maxi-
WARNING: XYLENE IS FLAMMABLE. mize disinfection.

Q Using the provided plastic scraper, remove

solidified paraffin from the gray insulated
work space and hot plate area.

Q As necessary, wipe the insulated work space

and hot plate area with a xylene-dampened
cloth.

Q Clean the grooves surrounding the hot plate

areas with a xylene-dampened cotton swab.

7.2 Revised 07/12/11

PERIODIC MAINTENANCE 4. WARNING: BEFORE PROCEEDING,
(AS REQUIRED) MAKE SURE THE HEAT KEY IS OFF.

Allow paraffin (in chamber where tray was locat-

Embedding Module ed) to solidify. Using the plastic scraper, remove
as much solidified paraffin as possible from this
‹ CLEAN TRANSFER TRAY (IF USED AS
chamber.
THE PARAFFIN BATH); ADD CLEAN
PARAFFIN
5. WARNING: XYLENE IS FLAMMABLE. BE
SURE MODULE IS COOL BEFORE USING
1. Allow paraffin in transfer tray to solidify.
XYLENE.
NOTE: Check to make sure AUTO/MAN key is
Wipe transfer tray and appropriate chamber with
set to MAN (MAN LED is illuminated) and the
xylene-dampened cloth. Dry transfer tray and
HEAT key is OFF. If using automatic timer, per-
chamber with a soft, clean, dry cloth.
form this procedure during a time period when
module is not set to come on. NOTE: To add paraffin, refer to “Adding Paraffin” in
Section 3 of this manual.
2. After paraffin is solidified, press
AUTO/MAN key to MAN (MAN LED is
illuminated) and press the HEAT key ON.

Allow paraffin between chamber and transfer

tray to liquefy (just enough to be able to
remove tray); remove tray. Press the HEAT
key OFF.

3. Turn tray upside down over a waste container

and “slightly twist” tray to eject solidified
paraffin.

Revised 07/12/11 7.3

CARE OF THE INSTRUMENT

‹ CLEAN THE FOLLOWING (AND THEN Using a clean cloth, remove loose debris from
ADD CLEAN PARAFFIN WHERE the chamber used to store/heat base molds.
APPROPRIATE)
7. Using a xylene-dampened cloth, wipe exterior
Paraffin Chamber surfaces, ventilation panels and inside surfaces
Paraffin Filter of paraffin chamber, paraffin bath, base mold
Forceps Wells storage chamber and waste drawer.
Exterior Surfaces
Waste Drawer Wipe each forceps well with a xylene-dampened
Paraffin Bath (right or left chamber) cotton swab.
Chamber Storing/Heating Base Molds
WARNING: XYLENE IS FLAMMABLE. BE
WARNING: MOLTEN PARAFFIN MAY SURE THE MODULE IS COOL PRIOR TO
CAUSE BURNS. USING XYLENE.

1. Allow solidified paraffin to liquefy 7. CAUTION:

7. (a) Do not pour xylene into paraffin cham-
NOTE: Press AUTO/MAN key to MAN and the ber. Xylene will damage the gasket and
HEAT key ON to turn heater ON if paraffin is sealing materials.
not molten. 7. (b) Excessive use (soaking) of xylene or other
organic solvents will destroy the painted
2. Using a cup (or similar device) remove as surfaces of each module. Minimize use of
much paraffin as possible from the paraffin such solvents on painted surfaces.
chamber and “right or left chamber” paraffin
bath. Do not tilt module. 8. Dry surfaces (as necessary) with a soft, clean,
dry cloth. Replace base molds.
3. Place container under paraffin dispenser.
Press fingerplate or optional foot pedal to 9. Using a curved tipped forceps (or similar
drain remaining paraffin from chamber. device) remove metal ring over paraffin filter in
paraffin chamber; then remove paraffin filter.
4. Using cotton swabs, remove liquefied paraf-
fin from each forceps well. Turn the HEAT 10. Remove as much foreign particulate matter and
key OFF. paraffin debris as possible from the paraffin fil-
ter.
5. WARNING: BEFORE PROCEEDING,
MAKE SURE THE HEAT KEY IS OFF. 11. Immerse the paraffin filter and metal ring in
xylene. Use a stiff nylon brush (or similar
Allow Embedding Module to cool; allow device) to remove remaining particulate matter
remaining paraffin on surfaces to solidify. from filter and metal ring.

Remove base molds from either right or left

chamber.

6. Remove solidified paraffin from exterior sur-

faces, waste drawer, paraffin bath and paraf-
fin chamber (use plastic scraper).

7.4 Revised 07/12/11

12. Dry paraffin filter and metal ring with a soft, To remove paraffin debris:
clean, dry cloth. 1. Dampen a soft cloth with xylene.

13. Replace paraffin filter. Replace metal ring (place 2. Wipe assembly to remove paraffin and other debris.
on top of filter).
2. WARNING: XYLENE IS FLAMMABLE. BE
WARNING: RETURN XYLENE AND SURE THE MODULE IS COOL PRIOR TO
XYLENE-DAMPENED CLOTH TO SAFE USING XYLENE.
STORAGE PRIOR TO RESTORING
POWER TO THE MODULE. 2. CAUTION: Do not immerse assembly in
xylene or frame surrounding lens may soften
NOTE: To add paraffin, refer to “Adding Paraffin” in or disintegrate.
Section 3 of this manual.
3. Dry assembly using a soft, clean, dry cloth.

‹ CLEAN MAGNIFYING LENS Cryo Module

ASSEMBLY (OBTAINED BY SEPARATE
PURCHASE) ‹ Clean Exterior Surfaces/Ventilation Panels
The magnifying glass and assembly may be 1. WARNING: SET POWER SWITCH ON
cleaned: FRONT PANEL OF CRYO MODULE TO
OFF. UNPLUG POWER CORD FROM
Q while still attached to the Embedding Module OUTLET.
or
Q when totally removed from Embedding 2. Using a clean cloth dampened in water or mild
Module. soap solution, remove dust/debris from exteri-
or surfaces, ventilation panels and work area
(surfaces under and around module).
To remove non-paraffin debris:
1. Dampen a soft cloth with household glass cleaner. 3. Rinse as necessary. Dry using a soft, clean, dry
cloth.
2. Wipe magnifying glass and entire assembly to
remove fingerprints, dust particles and other non- 4. Connect power cord to module. Plug power
paraffin debris. cord into outlet.

3. Dry entire assembly using a soft, clean, dry cloth.

Revised 07/12/11 7.5

CARE OF THE INSTRUMENT

MINOR REPAIR
Replacing Light Bulbs

Tools Required:
None

Light Bulbs Required:

Use product code 1553 to reorder. Two light bulbs
per package.

WARNING: Set the power switch on the front

panel of the Embedding Module to OFF. Unplug
the power cord. Allow module hot plate to cool to
room temperature.

1. Remove clear plastic shield protecting lights.

2. Grasp light bulb and pull downward to remove

light bulb. Repeat procedure for other light bulb
(if it also requires replacement).

WARNING: Avoid touching hot plate.

3. Grasp new light bulb and insert upwards into

socket. Repeat as required for other light bulb.

4. Plug power cord into wall outlet. Set power switch

on front panel to ON.

5. Press LIGHT key.

5. NOTE: To extend light bulb life, turn off light

when not embedding specimens.

7.6 Revised 07/12/11

SERVICE AND Section 8
REPLACEMENT PARTS
SERVICE

When you have a problem with either

module:
Q Refer to “Troubleshooting” (Section 6).

If Section 6 cannot assist you in solving the prob-

lem, continue with “How To Report the Problem”
(next page).

Revised 07/12/11 8.1

SERVICE AND
REPLACEMENT PARTS
How to Report the Problem CRYO MODULE

STEP ONE 1. Is the cryo module Yes No

power cord plugged in?
Record the following information.
2. Is the power switch on the front Yes No
Embedding Module Serial No. panel of both Modules set to ON?
Cryo Module Serial No.
3. Are the “POWER” indicators lit? Yes No
Embedding Module Model No.
Cryo Module Model No. 4. Is the connecting cable linking Yes No
both modules inserted properly
Date of Installation at both ends?

5. Does the cooling plate reach Yes No

STEP TWO the correct temperature within
15 minutes?
Complete the following “Preservice Checklist”
for the particular module that has malfunctioned. 6. If step “5” is NO, what is the
lowest temperature reached?
EMBEDDING MODULE (record in blank) _______°C
1. Is the power cord plugged in? Yes No
STEP THREE
2. Is the power switch on the Yes No
front panel set to ON? Call 1-800-725-8723 for help (U.S. Customers,
only).
3. Is the “POWER” indicator lit? Yes No
If located outside the U.S., contact your nearest
4. Does the proper LED Yes No Tissue-Tek® instrument distributor or representative.
illuminate when the
MANUAL MODE ON/OFF
key is pressed?

5. Is an error message displayed? Yes No

6. Does the LCD display Yes No

function?

7. Do keys properly function? Yes No

8.2 Revised 07/12/11

REPLACEMENT PARTS ACCESSORIES
(Available for use with your Tissue-Tek Tissue
Part Part Number* Embedding Console System)
Plastic Scraper 1550
Tissue-Tek® Uni-Cassette®
Tamper, Large (3/case) 1551 Embedding Cassettes
Tamper, Small (3/case) 1552
Tissue-Tek® II
Light Bulbs (2/case) 1553 Processing Embedding Cassettes
150-Cassette Tissue-Tek® Base Molds
Transfer Tray 5781
Tissue-Tek® Mold Release Concentrate
65-Cassette (Product Code No. 4141, 12/case)
Transfer Tray 5782
Magnifying Lens Assembly
Operating Manual 0001338-01
(Product Code No. 5784)
Paraffin Drawer N59-043-00
Foot Pedal (Product Code No. 5785)
Embedding Power Cord
100V/115V A4-01-0500 Electric Forceps (Product Code No. 5786)
220V/240V A4-01-0502
Where To Order?
Cryo Power Cord In the U.S., accessory and pricing information is
100V A4-01-0503 available directly from:
220V A4-01-0504
Customer Service
Cryo Connecting cable A4-12-0001 Sakura Finetek U.S.A., Inc.
Embedding Module 1750 West 214th Street
115 VAC, America 5101 Torrance, CA 90501 USA
230 VAC, Europe 5230 or by calling
220-230 VAC, Asia 5233
115 VAC, Asia 5236 1-800-725-8723
100 VAC, Asia 5239 If located outside the U.S., contact your nearest
Cryo Module authorized Tissue-Tek instrument distributor
115 VAC, America 5102 for information on ordering accessories.
230 VAC, Europe 5231
220-240 VAC, Asia 5234
115 VAC, Asia 5237
100 VAC, Asia 5240

*part numbers are subject to change

without notice

Revised 07/12/11 8.3

SERVICE AND
REPLACEMENT PARTS
OTHER AVAILABLE TISSUE-TEK® Tissue-Tek® – Available for use with
EQUIPMENT and ACCESSORIES Uni-Cassette® mechanical imprinters and
for manual identification
An extensive line of Tissue-Tek equipment and
accessory items is available. Refer to the “Tissue- – 11 distincitive colors
Tek Systems Catalog”* for complete information
on each item listed below.
Tissue-Tek® – has 1 mm pores (no slots)
*Catalog is available in the U.S., by dialing 1-800-725-8723. Biopsy
If located outside the U.S., contact your nearest Tissue-Tek Cassette – reduces the need to wrap
instrument distributor or representative. specimens which reduces risk
of specimen loss

– seven contrasting colors assist

Equipment and in locating biopsy specimens
Accessories Features
Tissue-Tek® – increased depth to
Tissue-Tek® – processes and safeguards Mega-Cassette® accommodate larger
VIP™ up to 300 specimens specimens
Vacuum simultaneously
Infiltration – used with mega base molds
Processor – brings reagents to the
specimen (eliminates the Tissue-Tek® – six drawers accommodating
hazards of moving Filing about 250 cassettes per drawer
specimens) Cabinets
– data sheets affixed to each
– air handling system isolates drawer, lightweight
specimens from ambient air construction and molded
and keeps solvent fumes out ridges or stacking legs to
of the working area permit stacking cabinets on
top of one another
Tissue-Tek® – Timing Disk provides for
Rotary automatic processing
Tissue
Processor – handles approximately 100
specimens at a time

8.4 Revised 07/12/11