Chapter 6 - Nutrition (Vitamin C)
Chapter 6 - Nutrition (Vitamin C)
Chapter 6 - Nutrition (Vitamin C)
Aim/
Objective
Problem
statement
Hypothesis
Manipulated
Variable
Responding
Variable
Fixed Variable
Material
Apparatus
Technique
Procedure
1.
Three specimen tubes are labeled as ascorbic acid, lime juice and
pineapple juice.
1 ml of DCPIP solution is placed in each specimen tube.
A syringe is filled with 5 ml of ascorbic acid solution.
The needle of the syringe is immersed in the DCPIP solution.
The ascorbic solution is added drop by drop to the DCPIP solution and
the tube is shaken slowly.
The amount of ascorbic acid solution use to decolorize the DCPIP
solution is recorded.
Steps 2 to 6 are repeated using lime and pineapple juices.
The percentage and concentration of vitamin C in the in the fruit juices
are calculated. using the following
2.
3.
4.
5.
6.
7.
8.
9.
0.1% ascorbic acid solution, DCPIP solution, freshly prepared fruit juice
and pineapple juice
Specimen tubes, a syringe (1 ml), syringes (5ml) with needles, beakers
(50ml), gauze cloth, knife and mortar.
Results
:
Solution
Ascorbic
Acid
Lime
juice
Pineapple
juice
Conclusion
Note
Initial
Volume
(ml)
Final
Volume
(ml)
Volume
used
(ml)
Percentage Of
Vitamin C (%)
Concentration
Of Vitamin C
(mg ml)
5.0
4.0
1.0
5.0
2.5
2.5
1/2.5 x
0.1=0.04
0.4
5.0
1.4
3.6
1/3.6 x
0.1=0.03
0.3
Lime juice contain more vitamin C than pineapple juice. The hypothesis
is accepted
Formula Calculation:
Percentage of vitamin C= Volume of 0.1% ascorbic acid solution X 0.1
Volume of fruit juice
Concentration of Vitamin C = Volume of 0.1% ascorbic acid solution
Volume of fruit juice
The greater the volume of fruit juice needed to decolorize 1ml DCPIP
solution, the lower the content of vitamin C in the fruit juice