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    Experiment Vit C

    Uploaded by

    inadirah
    1. The document describes an experiment to determine which fruit juice (orange juice, calamansi juice, or lemon juice) contains the highest amount of vitamin C. 2. The experiment involves using a solution of ascorbic acid (vitamin C) and DCPIP (dye) to titrate samples of each fruit juice and calculate the concentration of vitamin C based on the volume needed to decolorize the dye. 3. Based on the results, the conclusion will identify which fruit juice has the highest concentration of vitamin C and thus the highest vitamin C content.

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    © All Rights Reserved
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    Experiment Vit C

    Uploaded by

    inadirah
    92 views4 pages
    1. The document describes an experiment to determine which fruit juice (orange juice, calamansi juice, or lemon juice) contains the highest amount of vitamin C. 2. The experiment involves using a solution of ascorbic acid (vitamin C) and DCPIP (dye) to titrate samples of each fruit juice and calculate the concentration of vitamin C based on the volume needed to decolorize the dye. 3. Based on the results, the conclusion will identify which fruit juice has the highest concentration of vitamin C and thus the highest vitamin C content.

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    experiment vit C

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    1. The document describes an experiment to determine which fruit juice (orange juice, calamansi juice, or lemon juice) contains the highest amount of vitamin C. 2. The experiment involves using a solution of ascorbic acid (vitamin C) and DCPIP (dye) to titrate samples of each fruit juice and calculate the concentration of vitamin C based on the volume needed to decolorize the dye. 3. Based on the results, the conclusion will identify which fruit juice has the highest concentration of vitamin C and thus the highest vitamin C content.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    92 views4 pages

    Experiment Vit C

    Uploaded by

    inadirah
    1. The document describes an experiment to determine which fruit juice (orange juice, calamansi juice, or lemon juice) contains the highest amount of vitamin C. 2. The experiment involves using a solution of ascorbic acid (vitamin C) and DCPIP (dye) to titrate samples of each fruit juice and calculate the concentration of vitamin C based on the volume needed to decolorize the dye. 3. Based on the results, the conclusion will identify which fruit juice has the highest concentration of vitamin C and thus the highest vitamin C content.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    Problem statement

    Which fruit juice has the highest content of vitamin C?

    Materials
    Orange juice, calamansi juice and lemon juice, 0.1% ascorbic acid solution, 1% DCPIP
    solution and distilled water
    Apparatus
    Syringe needles (1 ml and 5 ml), knife, 50 ml beaker, specimen tube, juicer and sieve

    Procedure
    1 Put 1 ml of 1% DCPIP solution in a specimen tube.
    2 Fill the 1 ml syringe needle with a 0.1% ascorbic acid solution. Ensure that there are no air
    bubbles trapped in it.
    3 Insert the tip of the syringe needle into the specimen tube and drip ascorbic acid drop by
    drop into the DCPIP solution while stirring slowly until the blue colour of the DCPIP solution
    is decolourised.
    4 Record the volume of the 0.1% ascorbic acid solution needed to decolourise the blue colour
    of the DCPIP solution.
    5 Repeat steps 1 to 4 twice to get the average volume for each different juice.
    6 Record the volume of each juice in the table below.
    7 Calculate the concentration of vitamin C of each juice using the following formula.
    Percentage of vitamin C = volume of ascorbic acid solution × 0.1%
    volume of juice used
    Vitamin C concentration (mg ml-1) = volume of ascorbic acid solution × 1.0 mg ml-1
    volume of juice used

    Results

    Discussion
    1 Which juice has the highest content of vitamin C?

    2 Why is a 0.1% ascorbic acid solution used as the standard?

    Conclusion
    Is the hypothesis accepted?

    Suggest a suitable conclusion for this experiment.


    Answer all questions
    1. Based on table result above, state one observation.
    ____________________________________________________________________
    ____________________________________________________________________

    2. State an inference which correspond to the obsevation in question 1.


    ____________________________________________________________________
    ____________________________________________________________________

    3. Complete the variables based on the experiment;

    Manipulated variable
    Responding variable
    Constant variable

    4. State the hypothesis for the experiment.

    _____________________________________________________________________
    _____________________________________________________________________

    5. Construct a table and record all the data collected from the experiment. Your table
    should have the following titles;
     Types of solution or fruit juice
     Average volume of solution or fruit juice used to decolorise 1ml of 0.1%
    DCPIP solution
     Concentration of vitamin C in mgml-1

    6. Use graph paper to answer this question.


    Using the data in table question 5, draw a bar chart of the concentration of vitamin C
    againts the types of fruit juices.

    7. Another group of students carried out the same experiment by using processed mango
    juice as shown in diagram.

    Predict the volume of processed mango juice that need to decolorised the DCPIP
    solution. Explain your answer.
    _____________________________________________________________________
    _____________________________________________________________________

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