Lecture 3
Lecture 3
Lecture 3
Enzyme X Enzyme X
5’-TTC- 5’-TTC-
3”-AAG- 3”-AAG-
*
*
*
*
* *
16S gene * * Cell
membrane
B Drift Slime Streamer
10 µm
DAPI FER656
Oligunucleotide design
FISH variations
• FISH-CARD – instead of a fluorescent
probe on oligo sequence, but another
molecule that can then bond to many
fluorescent probes – better signal-to-noise
ratio
• FISH-RING – design of oligo sequence to
specific genes – image all organisms with
DSR gene or nifH for example
Clone Library
• http://ocw.mit.edu/NR/rdonlyres/Civil-and-Environmental-Engineering/1-89Fall-2004/321BF8FF-75BE-4377-8D74-8EEE753A328C/0/11_02_04.pdf
http://www.ifa.hawaii.edu/UHNAI/NAIweb/presentations/astrobiol6.pdf