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    Color Plates (1-60)

    Uploaded by

    Gayle Bocala
    The document contains 48 color plates showing various biological concepts and processes related to immunology and molecular biology. The color plates depict structures like immunoglobulin molecules, agarose gel electrophoresis results, polymerase chain reaction processes, and other diagrams.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Color Plates (1-60)

    Uploaded by

    Gayle Bocala
    30 views12 pages
    The document contains 48 color plates showing various biological concepts and processes related to immunology and molecular biology. The color plates depict structures like immunoglobulin molecules, agarose gel electrophoresis results, polymerase chain reaction processes, and other diagrams.

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

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    The document contains 48 color plates showing various biological concepts and processes related to immunology and molecular biology. The color plates depict structures like immunoglobulin molecules, agarose gel electrophoresis results, polymerase chain reaction processes, and other diagrams.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    30 views12 pages

    Color Plates (1-60)

    Uploaded by

    Gayle Bocala
    The document contains 48 color plates showing various biological concepts and processes related to immunology and molecular biology. The color plates depict structures like immunoglobulin molecules, agarose gel electrophoresis results, polymerase chain reaction processes, and other diagrams.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 12

    .< ' .

    Color Plate 1 Color Plate 4

    Color Plate 2 Color Plate 5

    Color Plate 3 Color Plate 6


    Color Plate 7 Color Plate 1 0

    °
    Color Plate 8
    • «" 4
    Color Plate 11

    Color Plate 9 Color Plate 1 2


    *s

    Color Plate 13 Color Plate 16

    ijSSBf |8£
    Color Plate 14 Color Plate 17

    <f
    Color Plate 15
    o o q^ O O
    Color Plate 18
    i
    Color Plate 19 Color Plate 20

    Immunoglobulin
    Molecule
    Dimeric IgA Molecule
    Fab Fab

    Fab Fab
    Color Plate 21 Color Plate 22

    Tube N o . 1 2 3 4 5 6 7 8 9 1 0 1 1
    " N / ^ ' X / ^ ' N / ' ' " ^ / ' *

    Agglutination 0 1+ 2 + 4 + 4 + 3 + 3 + 2 + 1 + 1 + 0
    Color Plate 23
    Tube No. 1 2 3 4 5 6 7 8 9 1 0

    Agglutination Pos Pos Pos Pos Pos Pos Pos Pos Neg Neg
    Color Plate 24 Color Plate 25

    Color Plate 26 Color Plate 27

    'Wf ' A

    ' I ' r;
    Color Plate 28 Color Plate 29

    Color Plate 30 Color Plate 31


    „<
    J 2* 1*.
    *' %
    « 5
    X,
    II
    '•A
    Color Plate 32
    •
    Color Plate 35

    Color Plate 33 Color Plate 36

    * .fc*'
    ^ • *7< *.

    •
    ^'1
    Color Plate 34 Color Plate 37
    i ••
    •

    • %
    •

    Color Plate 38 Color Plate 41

    ,* MA. -r
    fr^~*

    "'

    Color Plate 39
    9 -%• ^v
    Color Plate 42

    Color Plate 40 Color Plate 43


    Color Plate 44

    r
    Color Plate 45 Color Plate 48

    o 1
    »

    Color Plate 46 Color Plate 49


    O 1
    Color Plate 50 Color Plate 53

    ^V

    o O
    €>•
    4$ 'mCrifra
    *•* §
    C O
    V *}** ^
    7 L•
    r ^^

    /-,
    w'Qo
    M
    Color Plate 54

    II
    •#g#' c>
    F°o
    re^^j
    Color Plate 52 Color Plate 55
    M B D
    Digest DNA with restriction enzyme
    Separate by electrophoresis
    5000 1 2 3 4 5
    4000
    3000 BH •• • E=I C=3
    2000 23kb ••

    1000 9.4 mm

    6.6
    900
    —
    800 4.1
    —
    700 I I 2.3
    2.1 -
    1 1
    600
    0.5
    —
    500 Chemical depurination
    Denaturation
    400
    Neutralization
    Transfer to membrane
    Color Plate 56 Bind DNA to membrane

    Hybridize membrane with 32P labeled probe


    Wash off excess probe
    Expose to X-ray film
    Develop autoradiogram
    1 2 3 4 5
    23kb—
    9.
    6.
    4.1
    2.
    2.1—

    0.5—

    Color Plate 57
    Cycle 1: Start with (1) Double-stranded DNA TEMPLATE ycle 2: Start with 2 double-stranded cDNA intermediates containing target sequence

    ' - G G C A A T A T T G C G A A G C C T G T C T - 3 '

    Step 1: Denaturation

    1A 5' -G G C A A T A ss TARGET C C T G T C T - 3'


    Step 1: Denaturation to 4 ssDNA templates
    -C C G T T
    1A 5'-G G C A A ss TARGET C T G T C T- 3'
    Step 2: Annealing of primers
    SS TARGET
    Reverse primer 5 ' G A
    G C A A T ss TARGET
    -G G C A A T A ss TARGET C C T G T C T- 3' ss TARGET
    Step 2: Annealing of primers
    ss Step 3: Extension of primers to form 4 double-stranded copies
    Step 3: Extension of primers

    I
    dNTPs:| 2 1 E C S • Buffer: 1A 5'-G G C A A ds TARGET C T G T C T- 3'
    3B 3'-C C G T T ds TARGET G [A C A
    KC1,
    IIIIIIIIIIIG G G Mg++, PH 8.3 2B C C G T T A T A A C G C T T C G G [A C A
    4A G C A A T A T T G C G A A G C C T G T
    1A 5' - G G C A A T A T T G C G A A C G T C T- 3'
    Precise length products become preferred templates (i.e., 4A & 4B)
    a
    4B C G T
    2A G C A A T A T T G C G A A G C C T G T C T - 3 '

    3A G C A A T ds TARGET C T G T C T-

    Cycle 3 to 30: Repeat 3 step cycle.


    End of cycle 1: 2 double stranded DNA products At the end of 30 cycles = 23( copies of target sequence
    Color Plate 58a
    Setc.A 5' G C A A T A T T G C G A A G C C T G T C
    Setc.B 3'

    Note: Precise length products are bounded by primer sequences


    Color Plate 58b
    M 1 2 3 4 5 + Neg

    500 bp
    250 bp

    Color Plate 59

    22 25

    600

    Color Plate 60

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