Chemistry Investigatory

Chemistry
Investigatory Project
Session 2024-2025
A Project Report on:-

“digestion of starch by salivary
amylase and effect of ph and
temperature on it.”
Submitted by Maryam Zia

From Class 12th DG
CERTIFICATE
This is to certify that “Maryam Zia”
student of class - 12th ‘Science’ ‘DG’ has
succesfully completed their Chemistry
project on “To study the digestion of
starch by salivary amylase and effect of ph
and temperature on it.”
Under the guidance of “Mrs Alpana
Srivastavas” during academic session
2024-2025.
ACKNOWLEDGEMENT
I would like to convey my heartfelt gratitude to
Mrs. Alpana Srivastavas for her tremendous
support and assistance in the completion of my
project. I would also like to thank our Principal,
Mr. RK. Alamgir Islam, for providing me with
this wonderful opportunity to work on the
Project . The completion of the project would
not have been possible without their help and
insights.
Maryam Zia
Class-12th DG
Table of Contents
v Certificate
v Acknowledgement
v Aim of Project
v Objective of the Report
v Introduction
v Apparatus required
v Procedure
v Observation
v Conclusion
v Precaution
v Bibliography
Aim
“To study the digestion of
starch by salivary
amylase and effect of ph
and temperature on it.”
Objective of the Report
The main objective of this
chemistry project report is :-
“To Study the
digestion of starch by
salivary
amylase and effect of
temperature and pH on it.”
1) To study digestion of
starch by saliva.
2) To study the effect of
temperature on the
digestion of starch by saliva.
3)To study the affect of ph on
salivary digestion of starch.
Introduction
Every Health book insists on the chewing
of food. The act of chewing stimulates
the excretion of saliva. Saliva mixes up
with the food and helps its
digestion.That is, the enzyme salinity or
amylase present in human saliva
hydrolyzes the big molecules of food into
many molecules. For example starch into
mono-saccharine maltose and glucose;
proteins into amino acids and fats into
fatty acids and glycerol. Thus saliva not
only helps in digestion of food but
convert it into energy generating
substances. Further, enzymes and their
activity are very sensitive to temperature
and ph. Even a slight variation in these
two factors, can disturb the action of
enzymes. In other words,digestion of food
by salivary amylase is also effected by
pH and temperature and can be verified
experimentally. For example, hydrolysis
of starch can be verified by testing it
with iodine solution. Starch forms blue
coloured complex with iodine. If no
starch is present in a system it will not
give blue colour with iodine.
Apparatus Required
The requirements for
experiment of Chemistry
Project Report are as follows :
1)Test tubes
2)Test tube stand
3)One dropper
4)Beaker
5)Stop watch
6)Starch and Iodine solution
7)Thermometer
8)Dilute HCL and Dilute
NaOH solution
Experiment - 1
Procedure for Chemistry Experiment
1 is :-
1)Collection of Saliva :– Rinse

mouth thoroughly with cold water
and ensure that it does not contain
any food particles. Now take about
20ml of Luke warm water in the
mouth and gargle for about three
minutes so that saliva mixes up well
with it. Spit this into a beaker. Filter,
if there is any suspended impurity
clear filtrate is saliva solution and
contains enzyme salinity.
2)Preparation of starch solution :–

Take about 0.5g of starch in 100ml
beaker and add enough water to
make a paste. Dilute the paste by
adding 50ml water and boil for
about 5 minutes.
3)Digestion of starch :-
(a)Take 5ml of the starch solution

in a test tube. Add 2ml of saliva
solution into it. Mix the solutions
well by shaking the tube carefully
and start a step watch.
(b)After one minute take out two

drops of the mixture solution from
the test tube with the help of a
dropper and transfer it into
another test tube containing about
one 1ml of 1% iodine solution. Note
the colour produced, if any.
(c)Repeat this test after every one

minute taking two drops of the
mixture solution and fresh 1%
iodine solution continue until the
test shows no blue colour. Record
the time and blue colour intensity.
Observation
Time passed 1 minute 2 minute 3 minute 4 minute
after mixing
Colour Deep blue Blue Light blue No blue

intensity
Absence of blue colour on addition

to iodine solution means absence
of starch in the mixture
solution.That is whole of the
starch has got digested or
hydrolyzed.
Experiment - 2
Procedure:- Effect of temperature on
the digestion of starch by saliva.
1)Take three test tubes and label these

1, 2, and 3.
2)Take 5ml of the starch solution, 2ml
of the saliva solution and 5ml of water
in each test tube.
3)Place test tube Number 1 in water at
room temperature, test tube Number 2
in a beaker containing water at 500 C
and test tube Number 3 in boiling
water.
4) After 5 minutes, observe the colour
change on mixing two drops of the
mixture of every tube with one ml of
1% iodine solution. Note the intensity
of blue coloured form.
Conclusion :-
Starch get hydrolyzed by salivary
amylase.
Experiment - 3
Procedure: - To study the effect of pH on
the salivary digestion of starch.
1)Take three test tubes and label these 1,

2, and 3.
2) Add 5ml of the starch solution, 2ml of
the saliva solution in each test tube.
3) Now ass 2ml of water in test tube
Number 1 2ml of Dilute HCL in test tube
Number 2 and 2ml of Dilute NaOH
solution in test tube Number 3 and shake
carefully.
4) Keep the three test tubes in water at
room temperature for about 10 minutes.
5) Add 2 drops of the solution of each test
tube with 1% iodine solution and observe
the colour change.
Conclusion:-
Temperature effects the digestion of starch
by salivary with increase in temperature
salivary amylase get inactivated and
process of digestion do not take place.
Bibliography
1)www.google.com
2)Ncert class 12
3)Wikipedia.org
4)Comprehensive chemistry

Chemistry Investigatory

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Chemistry

A Project Report on:-

Submitted by Maryam Zia

1)Collection of Saliva :– Rinse

2)Preparation of starch solution :–

(a)Take 5ml of the starch solution

(b)After one minute take out two

(c)Repeat this test after every one

Colour Deep blue Blue Light blue No blue

Absence of blue colour on addition

1)Take three test tubes and label these

1)Take three test tubes and label these 1,

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