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NARAYANA E
TECHNO
SCHOOL
CHEMISTRY PROJECT
SESSION 2023-2024
A Project Report On
“Digestion of starch by salivary amylase
and effect of temperature and pH on it”
Submitted by Likith Reddy B V

Class – XIIth (Science)
Roll no. (cbse) – ______
Under the
Guidance of
Table of Contents
 Certificate
 Declaration
 Acknowledgement
 Aim of Project
 Objective of the Project Report
 Introduction
 Apparatus Required
 Procedure
 Observation
 Conclusion
 Precaution
 Bibliography
NARAYANA E TECHNO SCHOOL

Certificate
This is to certify that LIKITH REDDY B V student of class XIIth has
successfully prepared the report on the Project entitled “To
study the digestion of starch by salivary amylase and effect of
temperature and pH on it” under the guidance of
The report is the result of his efforts & endeavours. The

report is found worthy of acceptance as final Project
report for the subject Chemistry of class XIIth.
Signature of Chemistry Teacher Signature of External Examiner
--------------------------- --------------------------
Signature of Principal
-----------------------------
Declaration
I hereby declare that the project work entitled
“To study the digestion of starch by salivary
amylase and effect of temperature and pH on
it”, submitted to Department of chemistry,
NARAYANA E TECHNO SCHOOL is prepared by
me.
LIKITH REDDY
BV Class: XIIth (Science)
Acknowledgement
I would like to express a deep sense of thanks and gratitude to
my project guide Mr. S.P. Gupta sir for guiding me immensely
through the course of the project. He always evinced keen
interest in my project. His constructive advice & constant
motivation have been responsible for the successful completion of
his project.
My sincere thank goes to our principal sir for his co-ordination in

extending every possible support for the completion of this
project.
I must thanks to my classmates for their timely help and support

for completion of this project.
Last but not the least, I would like to thank all those who had
helped directly or indirectly towards the completion of this
project.
LIKITH REDDY B V
CLASS XIIth
AIM
“To study the digestion of
Starch by salivary amylase
and effect of temperature
and pH on it.”
Objective of the Project
Report
The main objective of this chemistry project
report is “To study the digestion of starch by
salivary amylase and effect of temperature and
pH on it”.
1. To study the digestion of starch by saliva.
2. To study the effect of temperature on the
digestion of starch by saliva.
3. To study the effect of pH on the salivary
digestion of starch.
Introduction
Every health book insists on the chewing of food. The act of chewing
stimulates the excretion of saliva. Saliva mixes up with the food and
helps its digestion. That is, the enzyme ptyalin or amylase present in
human saliva hydrolyses the big molecules of food into many
molecules.
For example, starch into mono-saccnaricies maltose and glucose;

proteins into amino acids and fats into fatty acids and glycerol.
Thus, saliva not only helps in digestion of food but also convert into
energy generating substances. Further, enzyme and their activity are
very sensitive to temperature and pH. Even a slight variation in these
two factors, can disturb the action of enzymes. In other words,
digestion of food by salivary amylase is also affected by temperature
and pH and can be verified experimentally.
For example, hydrolysis of starch can be verified by testing it

with iodine solution. Starch forms blue coloured complex
with iodine. If no starch is present in a system, it will not give
blue colour with iodine.
Apparatus Required
The requirements for experiment of chemistry project report Are as
follow:
1. Test tubes
2. Test tube stand
3. One dropper
4. Beaker
5. Stop watch
6. Starch
7. Iodine solution
8. Thermometer
9. Dilute HCl solution
10. Dilute NaOH solution
Experiment 1
Aim: - To study the digestion of starch by saliva
Procedure for Chemistry Experiment 1 is:
1. Collection of Saliva: - Rinse mouth thoroughly with cold water

and ensure that it does not contain any food particles. Now
take about 250ml of lukewarm water in the mouth and keep
for about three minutes so that saliva mixes up well with it.
Spit this into a beaker. Filter, if there is any suspended
impurity clear filtrate is saliva solution and contains enzyme
ptyalin.
2. Preparation of starch solution: - Take about 0.5g of starch in
100ml beaker and add enough water to make a paste. Dilute
the paste by adding 50ml water and boil for about 5 minutes.
3. Digestion of starch: -
a. Take 5ml of the starch solution in a test tube. Add 2ml of
saliva solution into it. Mix the solution well by shaking
the tube carefully and start a step watch.
b. After one minute, take out two drops of the mixture
solution from the test tube with the help of dropper and
transfer it into another test tube containing about 1ml of
1% iodine solution. Note the colour produced, if any.
c. Repeat this test after every one minute taking two

drops of the mixture solution and fresh 1% iodine solution
continue until the test shows no blue colour.
d. Record the time and blue colour intensity.
Observation
Time Passed 1 2 3 4
after Mixing minute minute minutes minute
s
Colour Intensity Deep Blue Light Blue No Blue
Blue
Absence of blue colour on addition to iodine solution means absence

of starch in the mixture solution. That is whole of the starch has got
digested or hydrolysed.
Conclusion
Starch gets hydrolysed by salivary amylase
Experiment 2
Aim: - To study the effect of temperature on the digestion

of starch by saliva.
1. Take three test tubes and label these 1,2, and 3.

2. Take 5ml of the starch solution, 2ml of the saliva solution and
5ml of water in each test tube. And shake the test tubes
carefully.
3. Place test tube number 1 in water at room temperature, test
tube number 2 in a beaker containing water at 50 C and test
tube number 3 in boiling water.
4. After 5 minutes, observe the colour change on mixing two
drops of the mixture of every tube with one ml of 1% iodine
solution. Note the intensity of blue coloured form.
Observation
Time passed after Intensity of blue colour
mixing saliva Test tube 1 at room
solution with starch temperature Test tube 2 Test tube 3 in
solution (in min) at 50°𝑪 boiling water
5 Light blue Blue Deep blue
6 No blue colour Light blue Blue
7 - Faint blue Blue
8 - No blue Blue
colour
9 - - Blue
10 - - Blue
Conclusion
Temperature affects the digestion of starch by saliva.
Experiment 3
Aim: - To study the effect of pH on the salivary digestion of
starch.
1. Take three test tubes and label these 1,2, and 3.
2. Add 5ml of the starch solution, 2ml of the saliva solution in
each test tube.
3. Now as 2ml of water in test tube number 1 2ml of Dilute HCl
in test tube number 2 and 2ml of Dilute NaOH solution in test
tube number 3 and shake carefully.
4. Keep the three test tubes in water at room temperature for
about 10 minutes.
5. Add 2 drops of the solution of each test tube with 1% iodine
solution and observe the colour change.
Observation
Colour produced (in
Test tube Solution taken test tube) with 1%
iodine
solution
1 Starch + saliva + No blue colour
water
2 Starch + saliva +dil. Blue colour
HCl
3 Starch + saliva + dil. Blue colour
NaOH
Conclusion
Hydrolysis of starch by the enzyme does not take place in the
acidic or alkaline medium.
Precaution
1. All apparatus should be clean and washed properly.

2. Add equal drops of iodine in all the samples while testing for
starch.
3. Temperature variation affects enzyme activity, so take the
readings at a constant temperature for all the samples.
4. Delay in sampling or the reaction time may affect the result.
So, iodine drops must be added at fixed intervals in all
samples.
Bibliography
1. Wikipedia-the free encyclopaedia

2. http://www.google.com/
3. http://en.wikipedia.org
4. www.icbse.com
5. Comprehensive Chemistry
6. Chemistry NCERT Class XIIth
7. Dinesh chemistry
Thank you!!
-LIKITH REDDY
BV

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NARAYANA E

Submitted by Likith Reddy B V

NARAYANA E TECHNO SCHOOL

The report is the result of his efforts & endeavours. The

Signature of Chemistry Teacher Signature of External Examiner

“To study the digestion of starch by salivary

amylase and effect of temperature and pH on

it”, submitted to Department of chemistry,

NARAYANA E TECHNO SCHOOL is prepared by

My sincere thank goes to our principal sir for his co-ordination in

I must thanks to my classmates for their timely help and support

For example, starch into mono-saccnaricies maltose and glucose;

For example, hydrolysis of starch can be verified by testing it

Aim: - To study the digestion of starch by saliva

Procedure for Chemistry Experiment 1 is:

1. Collection of Saliva: - Rinse mouth thoroughly with cold water

c. Repeat this test after every one minute taking two

Absence of blue colour on addition to iodine solution means absence

Aim: - To study the effect of temperature on the digestion

Procedure for Chemistry Experiment 2 is:

1. Take three test tubes and label these 1,2, and 3.

1. All apparatus should be clean and washed properly.

1. Wikipedia-the free encyclopaedia

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