Chemistry project for Class 12

1. 1. INVESTIGATORY PROJECT WORK Submitted By SHAHBAN ALI Class: XII Science Under the Guidance of
Mr. S.P Gupta PGT (Chemistry) Department of Chemistry Kendriya Vidyalaya Khagaria.
2. 2. Department of Chemistry Kendriya Vidyalaya Khagaria. C E R T I F I C A T E This is to certify that SHAHBAN
ALI of Class XII Science has prepared the investigatory chemistry project entitled “To Study the digestion of starch
by salivary amylase and effect of temperature and pH on it”. The report is the result of his efforts & endeavors. The
report is found worthy of acceptance as final project report for the subject Chemistry of Class XII. He has prepared
the report under my guidance. (Mr. S.P. Gupta) PGT (Chemistry) Department of Chemistry Kendriya Vidyalaya
Khagaria.
3. 3. Department of Chemistry Kendriya Vidyalaya Khagaria C E R T I F I C A T E The investigatory project entitled
“To Studythe digestion of starch by salivary amylase and effectof temperature and pH on it” Submitted by
SHAHBAN ALI of Class XII Science for the C.B.S.E Senior Secondary Examination class XII of Chemistry
Practical at Kendriya Vidyalaya Khagaria has been examined. SIGNATURE OF EXAMINER
4. 4. D E C L A R A T I O N I hereby declare that the project work entitled “To Study the digestion of starch by
salivary amylase and effect of temperature and pH on it”, submitted to Department of Chemistry, Kendriya
Vidyalaya Khagaria is prepared by me. SHAHBAN ALI Class XII Science
5. 5. A C K N O W L E D G E M E N T I would like to express a deep sense of thanks & gratitude to my project guide
Mr. S.P. Gupta Sir for guiding me immensely through the course of the project. He always evinced keen interest in
my work. His constructive advice & constant motivation have been responsible for the successful completion of this
project. My sincere thanks go to Shri Yeshdeep Rohilla, Our principal sir, for his co-ordination in extending every
possible support for the completion of this project. I also thanks to my parents for their motivation & support. I must
thanks to my classmates for their timely help & support for compilation of this project. Last but not the least; I would
like to thank all those who had Helped directly or indirectly towards the completion of this project. SHAHBAN ALI
6. 6. Class: XII Science
7. 7. CONTENTS 1. Certificateby Teacher. . . . . . . . . . . . . . . 2. Certificateby Examiner. . . . . . . . . . . . . . 3.
Declaration.. . . . . . . . . . . . . . . . . . . . . . . 4. Acknowledgement... . . . . . . . . . . . . . . . . 5. Verification.. . . . . . . . . . . . .
. . . . . . . . . . 6. Objectives of the Project. . . . . . . . . . . . . 7. Introduction. . . . . . . . . . . . . . . . . . . . . .
8. 8. . 8. MaterialRequired . . . . . . . . . . . . . . . . . . 9. Procedure. . . . . . . . . . . . . . . . . . . . . . . . . 10. Observation . . . . . .
. . . . . . . . . . . . . . . . . 11. CONCLUSION. . . . . . . . . . . . . . . . . . . . . . 12. BIBLIOGRAPHY. . . . . . . . . . . . . . . . . . . . .
9. 9. Objectives of the Project Report The main objective of this chemistry project report is “To Study the digestion of
starch by salivary amylase and effect of temperature and pH on it” and 1) To study digestion of starch by saliva. 2)
To study the effect of temperature on the digestion of starch by saliva. 3) To study the effect of pH on the salivary
digestion of starch.
10. 10. Introduction Every Health book insists on the chewing of food. The act of chewing stimulates the excretionof
saliva. Saliva mixes up with the food and helps its digestion. That is, the enzyme ptyalin or amylase present in
human saliva hydrolyzes the big moleculesof food into many molecules. For example starch into mono-
saccnaricies maltose and glucose; proteins into amino acids and fats into fatty acids and glycerol.Thus saliva not
only helps in digestion of food but convert it into energy generating substances. Further, enzymes and their activity
are very sensitive to temperature and ph. Even a slight variationin these two factors, can disturb the action of
enzymes. In other words, digestion of food by salivary amylase is also effected by pH and temperature and can be
verified experimentally.For example, hydrolysis of starch can be verified by testing it with iodine solution. Starch
forms blue coloured complex with iodine. If no starch is present in a system it will not give blue colour with iodine.
11. 11. Material Required The requirementsfor experimentof Chemistry ProjectReportare as under: 1) Test tubes 2)
Test tube stand 3) One dropper 4) Beaker 5) Stop watch 6) Starch and Iodine solution 7) Thermometer 8) Dilute
HCL and Dilute NaOH solution.
12. 12. Chemistry Experiment 1 Procedure Procedure for Chemistry Experiment 1 is: 1) Collection of Saliva – Rinse
mouth throughly with cold water and ensure that it does not contain any food particles. Now take about 20ml of
lupe warm water in the mouth and gangle for about three minutes so that saliva mixes up well with it. Spit this into
a beaker. Filter, if there is any suspended impurity clear filtrate is saliva solution and contains enzyme ptyalin. 2)
Preparation of starch solution – Take about 0.5g of starch in 100ml beaker and add enough water to make a paste.
Dilute the paste by adding 50ml water and boil for about 5 minutes. 3) Digestion of starch :- (a) Take 5ml of the
starch solution in a test tube. Add 2ml of saliva solution into it. Mix the solutions well by shaking the tube carefully
and start a step watch. (b) After one minute take out two drops of the mixture solution from the test tube with the
help of a dropper and transfer it into another test tube containing about one 1ml of 1% iodine solution. Note the
colour produced, if any. (c) Repeat this test after every one minute taking two drops of the mixture solution and
fresh 1% iodine solution continue until the test shows no blue colour. Record the time and blue colour intensity.
13. 13. Observation Time Passed after Mixing 1 Minute 2 Minute 3 Minute 4 Minute Colour Intensity Deep Blue Blue
Light Blue No Blue Absence of blue colouron addition to iodine solution means absence of starch in the mixture
solution.That is whole of the starch has got digested or hydrolysed.
14. 14. Chemistry Experiment 2 Procedure: - Effect of temperature on the digestion of starch by saliva. 1) Take three
test tubes and label these 1, 2, and 3. 2) Take 5ml of the starch solution, 2ml of the saliva solution and 5ml of
water in each test tube. 3) Place test tube Number 1 in water at room temperature, test tube Number 2 in a beaker
containing water at 500 C and test tube Number 3 in boiling water. 4) After 5 minutes, observe the colour change
on mixing two drops of the mixture of every tube with one ml of 1% iodine solution. Note the intensity of blue
coloured form. Conclusion Starch get hydrolysed by saliva amylase
15. 15. Chemistry Experiment 3 Procedure: - To study the effect of pH on the salivary digestion of starch 1) Take three
test tubes and label these 1, 2, and 3. 2) Add 5ml of the starch solution, 2ml of the saliva solution in each test tube.
3) Now ass 2ml of water in test tube Number 1 2ml of Dilute HCL in test tube Number 2 and 2ml of Dilute NaOH
solution in test tube Number 3 and shake carefully. 4) Keep the three test tubes in water at room temperature for
about 10 minutes. 5) Add 2 drops of the solution of each test tube with 1% iodine solution and observe the colour
change Conclusion Temperature effects the digestion of starch by salivary with increase in temperature salivary
amylase get inactivated and process of digestion do not take place.
16. 16. BIBLIOGRAPHY 1 http://www.google.com/ 2 http://en.wikipedia.org 3 N.C.E.R.T Class 12 Chemistry 4 Dinesh
Chemistry 5 Comprehensive Chemistry