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    Disusi 10

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    Zaqina Erin Setya Fazri
    1. The document describes the preparation and calculation of standard working solutions from a stock solution with a concentration of 5034 ppm. Six working standard solutions were prepared with concentrations ranging from 201.36 to 805.44 ppm. 2. A 500 ppm standard solution was also prepared for recovery calculations. Three samples were weighed and found to have masses ranging from 0.0213 to 0.0219 g. 3. Three recovery samples were also weighed and had masses ranging from 0.0209 to 0.0217 g. Linear regression of the standard solutions yielded a correlation coefficient of 0.9324. Concentrations of the samples and recoveries were calculated from the regression equation.

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    Disusi 10

    Uploaded by

    Zaqina Erin Setya Fazri
    18 views8 pages
    1. The document describes the preparation and calculation of standard working solutions from a stock solution with a concentration of 5034 ppm. Six working standard solutions were prepared with concentrations ranging from 201.36 to 805.44 ppm. 2. A 500 ppm standard solution was also prepared for recovery calculations. Three samples were weighed and found to have masses ranging from 0.0213 to 0.0219 g. 3. Three recovery samples were also weighed and had masses ranging from 0.0209 to 0.0217 g. Linear regression of the standard solutions yielded a correlation coefficient of 0.9324. Concentrations of the samples and recoveries were calculated from the regression equation.

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    1. The document describes the preparation and calculation of standard working solutions from a stock solution with a concentration of 5034 ppm. Six working standard solutions were prepared with concentrations ranging from 201.36 to 805.44 ppm. 2. A 500 ppm standard solution was also prepared for recovery calculations. Three samples were weighed and found to have masses ranging from 0.0213 to 0.0219 g. 3. Three recovery samples were also weighed and had masses ranging from 0.0209 to 0.0217 g. Linear regression of the standard solutions yielded a correlation coefficient of 0.9324. Concentrations of the samples and recoveries were calculated from the regression equation.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    18 views8 pages

    Disusi 10

    Uploaded by

    Zaqina Erin Setya Fazri
    1. The document describes the preparation and calculation of standard working solutions from a stock solution with a concentration of 5034 ppm. Six working standard solutions were prepared with concentrations ranging from 201.36 to 805.44 ppm. 2. A 500 ppm standard solution was also prepared for recovery calculations. Three samples were weighed and found to have masses ranging from 0.0213 to 0.0219 g. 3. Three recovery samples were also weighed and had masses ranging from 0.0209 to 0.0217 g. Linear regression of the standard solutions yielded a correlation coefficient of 0.9324. Concentrations of the samples and recoveries were calculated from the regression equation.

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    © All Rights Reserved
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    of 8

    1.

    Kosentrasi Larutan Baku


    botol timbang + ekstrak = 13,3952 g
    Botol timbang = 13,1435 g _
    Bobot ektrak = 0,2517 g

    Penimbangan dan konsentrasi Baku induk


    Baku induk 1 = 0,2517 g ~251,7 mg
    251 ,7 mg
    = × 1000=5034 ppm
    50 ml
    Baku induk 2 = N1 x V1 = N2 x V2
    5034 ppm x 4,0 ml = N2 x 10,0 ml
    N2 = 2013,6 ppm
    Konsentrasi baku kerja
    BK 6 : N1 x V1 = N2 x V2
    2013,6 ppm x 4,0 ml = N2 x 10,0 ml
    N2 = 805,44 ppm
    BK 5 : N1 x V1 = N2 x V2
    2013,6 ppm x 3,0 ml = N2 x 10,0 ml
    N2 = 604,08 ppm
    BK 4 : N1 x V1 = N2 x V2
    5034 ppm x 5,0 ml = N2 x 50,0 ml
    N2 = 503,4 ppm
    BK 3 : N1 x V1 = N2 x V2
    805,44 ppm x 5,0 ml = N2 x 10,0 ml
    N2 = 402,72 ppm
    BK 2 : N1 x V1 = N2 x V2
    604,08 ppm x 5,0 ml = N2 x 10,0 ml
    N2 = 302,04 ppm
    BK 1 : N1 x V1 = N2 x V2
    402,72 ppm x 5,0 ml = N2 x 10,0 ml
    N2 = 201,36 ppm
    2. Perhitungan Konsentrasi Larutan Standar EPMS (dibuat 500 ppm untuk recovery)
    botol timbang + ekstrak = 13,2013 g
    Botol timbang = 13,1495 g _
    Bobot ektrak = 0,0518 g ~ 51,8 mg
    51, 8 mg
    = × 1000=518 ppm
    100 ml

    3. Penimbangan sampel 20 mg → 0,02±10% (0,018 g -0,022 g )


     Sampel 1 → botol timbang + ekstrak = 12,8108 g
    Botol timbang = 12,7895 g _
    Bobot ektrak = 0,0213 g
     Sampel 2 → botol timbang + ekstrak = 12,6956 g
    Botol timbang = 12,6737 g _
    Bobot ektrak = 0,0219 g
     Sampel 3 → botol timbang + ekstrak = 13,1110 g
    Botol timbang = 13,0906 g
    Bobot ektrak = 0,0204 g

    4. Penimbangan recovery 20 mg → 0,02±10% (0,018 g -0,022 g )


     Recovery 1 → botol timbang + ekstrak = 12,9669 g
    Botol timbang = 12,9460 g _
    Bobot ektrak = 0,0209 g
     Recovery 2 → botol timbang + ekstrak = 12,2763 g
    Botol timbang = 12,2548 g _
    Bobot ektrak = 0,0215 g
     Recovery 3 → botol timbang + ekstrak = 13,1123 g
    Botol timbang = 13,0906 g _
    Bobot ektrak = 0,0217 g
    Tabel Konsentrasi dan Luas Area baku Kerja
    Konsentrasi (ppm) Luas Area (AV) Sampel dan
    BK Luas Area (AV)
    Recovery
    Teoritis Praktis (x) (y)

    1 200 201,36 14455,3 S1 20429,0

    2 300 302,04 25549,4 S2 21865,1

    3 400 402,72 21885,7 S3 21704,4

    4 500 503,40 22380,2 R1 18853,2

    5 600 604,08 22750,5 R2 20104,8

    6 800 805,44 25964,7 R3 19992,1

    Regresi 6 BK
    a : 16439,74
    b : 12,18406
    r hitung : 0,64 < r table (0,917)
    1 sampel direject (BK2)
    a : 12639,03
    b : 17,576976 ~ 17,577
    r hitung : 0,9324 < r table untuk 5 data

    Perhitungan Konsentrasi kadar Sampel dan Recovery


    a. S1
    y = 17,577x + a
    20429,0 = 17,577x + 12639,03
    x = 443,19 ppm
    b. S2
    y = 17,577x + a
    21865,1 = 17,577x + 12639,03
    x = 524,89 ppm
    c. S3
    y = 17,577x + a
    21704,4 = 17,577x + 12639,03
    x = 515,75 ppm
    d. R1
    y = 17,577x + a
    18853,2 = 17,577x + 12639,03
    x = 353,54 ppm
    e. R2
    y = 17,577x + a
    20104,8 = 17,577x + 12639,03
    x = 424,75 ppm
    f. R3
    y = 17,577x + a
    19992,1 = 17,577x + 12639,03
    x = 418,335 ppm

    Perhitungan sampel untuk penetapan kadar EPMS dalam ekstrak kering


    % kadar cab-o-sil = bobot cab-o-sil/ bobot ekstrak kering x 100%
    = 20 gram / 55,16 gram x 100% = 36,26%

    Penimbangan sampel
    Sampel 1 ektrak tanpa cab-o-sil = 21,3 mg - (36,26% x 21,3 mg) = 13,58 mg
    Sampel 2 ektrak tanpa cab-o-sil = 21,9 mg - (36,26% x 21,9 mg) = 13,96 mg
    Sampel 3 ektrak tanpa cab-o-sil = 20,4 mg - (36,26% x 20,4 mg) = 13,00 mg

    Pengenceran 3 ml
    3
    Sampel 1 : x 443 , 19 ppm = 1329,57 ppm
    1
    3
    Sampel 2 : x 524 , 89 ppm = 1574,67
    1
    3
    Sampel 3 : x 515 , 75 ppm =1547,25 ppm
    1
    Bobot sampel dalam 5 ml
    1329 ,57 mg
    Sampel 1 : x 5 ml = 6,6479 mg
    1000 ml
    1574 , 67 mg
    Sampel 2 : x 5 ml = 7,8734 mg
    1000 ml
    1547 ,25 mg
    Sampel 3 : x 5 ml = 7,7363 mg
    1000 ml

    (%) EPMS dalam sampel


    6,6479 mg
    Sampel 1 : x 100 % = 48,95%
    13 ,58 mg
    7,8734 mg
    Sampel 2 : x 100 % = 56,40%
    13 , 96 mg
    7,7363 mg
    Sampel 3 : x 100 % = 59,51%
    13 , 00 mg

    Penentuan Presisi
    48 , 95 %+ 56 , 40 %+ 59 ,51 %
    Rata-rata : = 54,95%
    3
    SD : 5,4266
    SD 5,4266
    KV : x 100 % = x 100 % = 9,88%
    rata−rata 54 , 95
    Penimbangan recovery
    Recovery 1 ektrak tanpa cab-o-sil = 20,9 mg - (36,26% x 20,9 mg) = 13,32 mg
    Recovery 2 ektrak tanpa cab-o-sil = 21,5 mg - (36,26% x 21,5 mg) = 13,70 mg
    Recovery 3 ektrak tanpa cab-o-sil = 21,7 mg - (36,26% x 21,7 mg) = 13,83 mg

    Pengenceran 4 ml
    4
    Recovery 1 : x 353 , 54 ppm = 1414,16 ppm
    1
    4
    Recovery 2 : x 424 , 75 ppm = 1699,00 ppm
    1
    4
    Recovery 3 : x 418,335 ppm = 1673,34 ppm
    1

    Bobot recovery dalam 5 ml


    1414 , 16 mg
    Recovery 1 : x 5 ml = 7,0708 mg
    1000 ml
    1699 ,00 mg
    Recovery 2 : x 5 ml = 8,4950 mg
    1000 ml
    1673 ,34 mg
    Recovery 3 : x 5 ml = 8,3667 mg
    1000 ml

    (%) EPMS dalam recovery


    7,0708 mg
    Recovery 1 : x 100 % = 53,08%
    13 , 32mg
    8,4950 mg
    Recovery 2 : x 100 % = 62,01%
    13 , 70 mg
    8,3667 mg
    Recovery 3 : x 100 % = 60,50%
    13 , 83 mg

    453 , 08 %+ 62 ,01 % +60 ,50 %


    Rata-rata : = 58,53%
    3
    Penentuan akurasi
     Ct : Kadar recovery dalam 5 ml
    rata−rata sampel x bobot penimbangan recovery
     Cp :
    100
    Ct
     Recovery : x 100 %
    Cp+ Cst

    Ct Cp Cst recovery

    54 , 95 x 13 , 32 7,0708
    7,0708 mg Cp1 = =7 , 32 0,518 R1 = x 100 %=90 ,21 %
    100 7 ,32+ 0,518

    54 , 95 x 13 , 70 8,4950
    8,4950 mg Cp2 = =7 , 53 0,518 R2 = x 100 %=105 ,55 %
    100 7 ,53+ 0,518

    54 , 95 x 13 , 83 8,3667
    8,3667 mg Cp3 = =7 , 60 0,518 R3 = x 100 %=103 , 06 %
    100 7 , 60+0,518

    90 , 21% +105 , 55 %+103 , 06 %


    Rata-rata : = 99,61%
    3

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