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    Data Pengamatan Dan Perhitungan HPLC PCT

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    Indarti Ulfayani
    This document contains data and calculations for chromatographic analysis. It presents results from tests of system suitability, including retention times and peak areas. It also describes preparation of standard and sample solutions for qualitative and quantitative analysis, including weighing amounts, making dilutions, and calculating concentrations. Finally, it shows the calibration curve generated from standard solutions, used to determine the concentration of the sample solution based on its peak area. The concentration was found to be 101.4% of the expected value based on calibration and 100.5% using a single point response.

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    Data Pengamatan Dan Perhitungan HPLC PCT

    Uploaded by

    Indarti Ulfayani
    221 views3 pages
    This document contains data and calculations for chromatographic analysis. It presents results from tests of system suitability, including retention times and peak areas. It also describes preparation of standard and sample solutions for qualitative and quantitative analysis, including weighing amounts, making dilutions, and calculating concentrations. Finally, it shows the calibration curve generated from standard solutions, used to determine the concentration of the sample solution based on its peak area. The concentration was found to be 101.4% of the expected value based on calibration and 100.5% using a single point response.

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    This document contains data and calculations for chromatographic analysis. It presents results from tests of system suitability, including retention times and peak areas. It also describes preparation of standard and sample solutions for qualitative and quantitative analysis, including weighing amounts, making dilutions, and calculating concentrations. Finally, it shows the calibration curve generated from standard solutions, used to determine the concentration of the sample solution based on its peak area. The concentration was found to be 101.4% of the expected value based on calibration and 100.5% using a single point response.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    221 views3 pages

    Data Pengamatan Dan Perhitungan HPLC PCT

    Uploaded by

    Indarti Ulfayani
    This document contains data and calculations for chromatographic analysis. It presents results from tests of system suitability, including retention times and peak areas. It also describes preparation of standard and sample solutions for qualitative and quantitative analysis, including weighing amounts, making dilutions, and calculating concentrations. Finally, it shows the calibration curve generated from standard solutions, used to determine the concentration of the sample solution based on its peak area. The concentration was found to be 101.4% of the expected value based on calibration and 100.5% using a single point response.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 3

    Data Pengamatan dan Perhitungan

    5.1 Uji Kesesuaian Sistem


    Fase gerak total : 200 mL
    Perbandingan : 3:1
    1
    Metanol pro HPLC : 4 x 200 mL = 50 mL
    3
    Aqua p.i : 4 x 200 mL = 150 mL

    Tabel 1. Uji Kesesuaian Sistem (Dari kel.A5)


    Penyuntikan Waktu Retensi Luas Area
    1 3,343 38994353
    2 3,33 32118351
    3 3,327 36980288
    4 3,323 38009655
    5 3,327 36742752
    6 3,317 37612217
    7 3,303 37368157
    Rata-rata 3,324285714 36832253
    Sd 0,01228433 2206327,5
    Sbr/rsd 0,369532916% 599,02%

    5.2 Analisis Kualitatif


    a. Larutan Standar
     Penimbangan : 25,3 mg
    25,3 𝑚𝑔
    x 1000 mL = 506 ppm
    50 𝑚𝐿

     Pengenceran
    𝑉1 . 𝑁1 = 𝑉2 . 𝑁2
    10 mL . 𝑁1 = 1 mL . 506 ppm
    𝑁1 = 50,6 ppm

    b. Larutan Uji
     Penimbangan : 25,2 mg
    25,2 𝑚𝑔
    50 𝑚𝐿
    x 1000 mL = 504 ppm
     Pengenceran
    𝑉1 . 𝑁1 = 𝑉2 . 𝑁2
    10 mL . 𝑁1 = 1 mL . 504 ppm
    𝑁1 = 50,4 ppm

    5.3 Analisis Kuantitatif


    a. Larutan Standar
     Penimbangan : 25,3 mg
    25,3 𝑚𝑔
    x 1000 mL = 506 ppm
    50 𝑚𝐿

     Pengenceran
    𝑉1 . 𝑁1 = 𝑉2 . 𝑁2
    − 10 mL . N1 = 0,2 mL . 506 ppm
    N1 = 10,12 ppm
    − 10 mL . N1 = 0,4 mL . 506 ppm
    N1 = 20,24 ppm
    − 10 mL . N1 = 0,6 mL . 506 ppm
    N1 = 30,36 ppm
    − 10 mL . N1 = 0,8 mL . 506 ppm
    N1 = 40,48 ppm
    − 10 mL . N1 = 1,0 mL . 506 ppm
    N1 = 50,6 ppm
    − 10 mL . N1 = 1,2 mL . 506 ppm
    N1 = 60,72 ppm
    b. Larutan Uji
     Penimbangan : 25,2 mg
    25,2 𝑚𝑔
    x 1000 mL = 504 ppm
    50 𝑚𝐿

     Pengenceran
    𝑉1 . 𝑁1 = 𝑉2 . 𝑁2
    10 mL . 𝑁1 = 1 mL . 504 ppm
    𝑁1 = 50,4 ppm
    5.4 Kurva Kalibrasi
    Table 2. Kurva Kalibrasi
    Konsentrasi (ppm) Luas Area
    10,12 14343130
    20,24 17001621
    30,36 24944611
    40,48 34454617
    50,6 41743361
    60,72 49467098

    a = 4390233,067
    b = 732227,7414
    r = 0,99238
    y = bx + a
    41807113 = 732227,7414x + 4390233,067
    37416879,93 = 732227,7414x
    37416879,93
    x = 732227,7414

    x = 51,1 ppm
    51,1 ppm
    %kadar = 50,4 ppm x 100%

    = 101,389% (hampir)
    5.5 One Point
    𝐿𝑢
    𝐶𝑢 = x 𝐶𝑠
    𝐿𝑠
    41807113
    𝐶𝑢 = x 50,6 ppm
    41743361

    𝐶𝑢 = 50,677 ppm
    50,677 ppm
    %kadar = x 100%
    50,4 ppm

    = 100,549%

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