MolBioLec Quiz3

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1. This RNA sequence is the sequence used to 11.

This mediates the transferring of Amino Acids to


determine what amino acids are produced the Aminoacyl-tRNA at the A site, becoming a
through mRNA. dipeptidyl- tRNA.
Coding Strand or Sense strand or Non- Peptidyl transferase
templating strand
12. This is the counterclockwise rotation of incident
2. How many STOP codons are present in an light in proteins.
organism? L configuration
Three
13. The configuration of proteins depends on the
3. To create a positive sense RNA from a negative location of what part of the Amino Acid
sense RNA, what enzyme facilitates this attached to the Alpha Carbon?
reaction? Amino group
RNA dependent RNA polymerase
14. The RNA chain transcribed will start on what
4. These are regions at the end of genes that end?
signal the end of transcription 5’ End
Terminator region
15. What is the end anticodon?
5. Protein that regulates transcription but itself is None
not a subunit of RNA polymerase
Transcription factor 16. Eukaryotic RNA polymerase are the only
capable of binding to___________ in the
6. During transcription, how many ribonucleotides presence of transcription factors.
must be added to the DNA-RNA hybrid when Promoters
the RNA polymerase starts to move?
9-10 nucleotides 17. This can couple transcription to processing of
RNA transcript to produce mature mRNA
7. This happens when the 2’OH of an Adenosine RNA Polymerase II
residue located within introns itself attack
previous exon- intron boundary, detaching 18. It is the factor where RNA polymerase II activity
intron from the previous exon has dependence on
RNA Splicing C- Terminal Domain

8. During translation in bacteria, this carries the 19. This is the first step in the termination of
uncharged tRNA transcription
E Site PAUSING

9. What is the start Amino Acid during 20. Approximately how many Adenine nitrogenous
transcription? bases are added in the 3’ tail of mRNA?
Methionine None or Zero (if asked, we add ADENOSINE,
not Adenine)
10. During termination, this attaches to the UAG in
human cells 21. These should be removed from the pre- mature
Release Factor mRNA before it goes out of the nucleus for
translation.
Introns
22. A group of three nucleotides in the DNA is 33. This is the structure that should be produced for
called the Polymerase to move out of the promoter
Base triplets RNA- DNA or DNA- RNA Stable Hybrid Complex

23. The direction of the RNA Polymerase for 34. What is the first base for the initiation of
Transcription elongation starts at what end? Transcription?
3’ end Adenine or Guanine

24. Amino acids forms a covalent bond with what 35. This factor guides the aminoacyl- tRNA into part
nucleotide in tRNA in the presence of of the A Site and aligns the anticodon with the
Aminoacyl- snthetase? mRNA codon
Adenine EF- Tu

25. What part of the mRNA does transcription 36. The part of the ribosome which contains the
start? active site where the peptide transfer and
Promoter region hydrolysis reactions occur and used for the
initiation of translation in eukaryotic cells. Give
26. The part of Ribosomal RNA that catalyzes the Svedberg units
peptide bond formation 60S
Large Ribosomal Subunit
37. The site for the cutting, folding, and shaping of
27. This is the location at which the aminoacyl- the translated proteins
tRNA anticodon base pairs up with the mRNA Endoplasmic Reticulum
codon
A Site or Aminoacyl Site 38. This helsps new polypeptides to fold properly to
avoid misfiling which may be inactive or deadly
28. Where the empty tRNA sits before released toxic to a cell
back into the cytoplasm to bind to another Molecular Chaperone
amino acid
E Site or Exit Site 39. The composition of rRNA in ribosomes
2/3
29. Give one posttranscriptional modification that
happens in prokaryotes that does not happen in 40. Because of the DNA is too large to leave the
Eukaryotic cells nucleus, what is formed in prokaryotes to
None facilitate protein synthesis?
None
30. Location of the rRNA where the Amino Acid is
transferred from its tRNA to the growing 41. This type of transcriptional modification is the
polypeptide chain removal of introns and ligation of exons bbefore
P Site or Peptidyl Site it goes outside of the nucleus
RNA Splicing
31. This is the elongation factor needed for 42. What type of sequence is found on tRNA that
translocation are specific to the mRNA?
EF- G Anticodons

32. A transcription bubble will move in the mRNA. 43. This type of bond holds 2 amino acids together
This movement will start at what end of the during Protein synthesis/
mRNA? Peptide bond
3’end
44. What is the start codon?
AUG
45. What is the start anticodon?
UAC
46. What is the start amino acid during translation?
Methionine
47. This transports amino acids in the ribosomes.
tRNA or transport RNA
48. What is the element present in the functional
center of an amino acid structure?
Carbon
49. This is called an antisense strand because its
code is the complement of the RNA that is
produced
Template Strand
50. What primer is needed during RNA synthesis?
None

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