Photosynthesis Ncellularrespiration
Photosynthesis Ncellularrespiration
Photosynthesis Ncellularrespiration
What is Photosynthesis?
The word photosynthesis can be separated to make two smaller words:
“photo” which means light
Plants need food but they do not have to wait on people or animals to provide for
them. Most plants are able to make their own food whenever they need it. This is done
using light and the process is called photosynthesis.
Photosynthesis is the process by which plants make their own food. We will add
more details to this definition after making a few things clear as you will see below.
What is needed for Photosynthesis?
To make food, plants need not just one but all of the following:
carbon dioxide
water
sunlight
Let’s take a look at how these are collected by plants.
Chlorophyll and other pigments absorb energy from sunlight. This energy is
transferred to the photosystems responsible for photosynthesis.
Water is used to provide electrons and hydrogen ions but also produces oxygen.
Do you remember what happens to the oxygen?
The electrons and hydrogen ions are used to create ATP and NADPH. ATP is
an energy storage molecule. NADPH is an electron carrier/donor molecule. Both
ATP and NADPH will be used in the next stage of photosynthesis.
Details about the flow of electrons through Photosystem II, b6-f complex,
Photosystem I and NADP reductasehave not been included here but can be found
under The Process of Photosynthesis in Plants.
The Calvin Cycle
The Calvin Cycle reactions occur in the stroma of the chloroplasts. Although these
reactions can take place without light, the process requires ATP and NADPH which
were created using light in the first stage. Carbon dioxide and energy from ATP along
with NADPH are used to form glucose.
The equation below is the same as the one above but it shows the chemical formula
for carbon dioxide, water, glucose and oxygen.
Oxygen that is produced during photosynthesis is released into the atmosphere. This
oxygen is what we breathe and we cannot live without it.
While it is important that photosynthesis provides food and oxygen, its impact on our
daily lives is far more extensive. Photosynthesis is so essential to life on earth that
most living organisms, including humans, cannot survive without it.
All of our energy for growth, development and physical activity comes from eating
food from plants and animals. Animals obtain energy from eating plants. Plants obtain
energy from glucose made during photosynthesis.
Photosynthesis is also responsible for balancing oxygen and carbon dioxide levels in
the atmosphere. Plants absorb carbon dioxide from the air and release oxygen during
the process of photosynthesis.
Lesson Objectives
Name the three stages of cellular respiration.
Explain why glycolysis probably evolved before the other stages of aerobic respiration.
List the steps of the Krebs cycle, and identify its products.
State the possible number of ATP molecules that can result from aerobic respiration.
Vocabulary
aerobic respiration
anaerobic respiration
glycolysis
Krebs cycle
Introduction
You have just read how photosynthesis stores energy in glucose. How do living things
make use of this stored energy? The answer is cellular respiration. This process
releases the energy in glucose to make ATP, the molecule that powers all the work of
cells.
Stages of Cellular Respiration
Cellular respiration involves many chemical reactions. As you saw earlier, the reactions
can be summed up in this equation:
The reactions of cellular respiration can be grouped into three stages: glycolysis, the
Krebs cycle (also called the citric acid cycle), and electron transport. Figure below gives
an overview of these three stages, which are also described below.
Cellular respiration takes place in the stages shown here. The process begins with a molecule of glucose, which has six carbon
atoms. What happens to each of these atoms of carbon?
Splitting Glucose
The word glycolysis means “glucose splitting,” which is exactly what happens in this
stage. Enzymes split a molecule of glucose into two molecules of pyruvate (also known
as pyruvic acid). This occurs in several steps, as shown in Figure below. You can
watch an animation of the steps of glycolysis at the following
link: http://www.youtube.com/watch?v=6JGXayUyNVw.
In glycolysis, glucose (C6) is split into two 3-carbon (C3) pyruvate molecules. This releases energy, which is transferred to ATP.
How many ATP molecules are made during this stage of cellular respiration?
Results of Glycolysis
Energy is needed at the start of glycolysis to split the glucose molecule into two
pyruvate molecules. These two molecules go on to stage II of cellular respiration. The
energy to split glucose is provided by two molecules of ATP. As glycolysis proceeds,
energy is released, and the energy is used to make four molecules of ATP. As a result,
there is a net gain of two ATP molecules during glycolysis. During this stage, high-
energy electrons are also transferred to molecules of NAD + to produce two molecules of
NADH, another energy-carrying molecule. NADH is used in stage III of cellular
respiration to make more ATP.
Anaerobic and Aerobic Respiration
Scientists think that glycolysis evolved before the other stages of cellular respiration.
This is because the other stages need oxygen, whereas glycolysis does not, and there
was no oxygen in Earth’s atmosphere when life first evolved about 3.5 to 4 billion years
ago. Cellular respiration that proceeds without oxygen is called anaerobic respiration.
Then, about 2 or 3 billion years ago, oxygen was gradually added to the atmosphere by
early photosynthetic bacteria. After that, living things could use oxygen to break down
glucose and make ATP. Today, most organisms make ATP with oxygen. They follow
glycolysis with the Krebs cycle and electron transport to make more ATP than by
glycolysis alone. Cellular respiration that proceeds in the presence of oxygen is
called aerobic respiration.
The structure of a mitochondrion is defined by an inner and outer membrane. This structure plays an important role in aerobic
respiration.
As you can see from Figure above, a mitochondrion has an inner and outer membrane.
The space between the inner and outer membrane is called the intermembrane space.
The space enclosed by the inner membrane is called the matrix. The second stage of
cellular respiration, the Krebs cycle, takes place in the matrix. The third stage, electron
transport, takes place on the inner membrane.
Cellular Respiration Stage II: The Krebs Cycle
Recall that glycolysis produces two molecules of pyruvate (pyruvic acid). These
molecules enter the matrix of a mitochondrion, where they start the Krebs cycle. The
reactions that occur next are shown in Figure below. You can watch an animated
version at this link: http://www.youtube.com/watch?v=p-k0biO1DT8.
The Krebs cycle starts with pyruvic acid from glycolysis. Each small circle in the diagram represents one carbon atom. For
example, citric acid is a six carbon molecule, and OAA (oxaloacetate) is a four carbon molecule. Follow what happens to the
carbon atoms as the cycle proceeds. In one turn through the cycle, how many molecules are produced of ATP? How many
molecules of NADH and FADH2 are produced?
Before the Krebs cycle begins, pyruvic acid, which has three carbon atoms, is split apart
and combined with an enzyme known as CoA, which stands for coenzyme A. The
product of this reaction is a two-carbon molecule called acetyl-CoA. The third carbon
from pyruvic acid combines with oxygen to form carbon dioxide, which is released as a
waste product. High-energy electrons are also released and captured in NADH.
Steps of the Krebs Cycle
The Krebs cycle itself actually begins when acetyl-CoA combines with a four-carbon
molecule called OAA (oxaloacetate) (see Figure above). This produces citric acid,
which has six carbon atoms. This is why the Krebs cycle is also called the citric acid
cycle.
After citric acid forms, it goes through a series of reactions that release energy. The
energy is captured in molecules of NADH, ATP, and FADH 2, another energy-carrying
compound. Carbon dioxide is also released as a waste product of these reactions.
The final step of the Krebs cycle regenerates OAA, the molecule that began the Krebs
cycle. This molecule is needed for the next turn through the cycle. Two turns are
needed because glycolysis produces two pyruvic acid molecules when it splits glucose.
Watch the OSU band present the Krebs cycle: http://www.youtube.com/watch?
v=FgXnH087JIk.
2 FADH2
The Krebs cycle is reviewed at http://www.youtube.com/watch?v=juM2ROSLWfw.
Cellular Respiration Stage III: Electron Transport
Electron transport is the final stage of aerobic respiration. In this stage, energy from
NADH and FADH2, which result from the Krebs cycle, is transferred to ATP. Can you
predict how this happens? (Hint: How does electron transport occur in photosynthesis?)
Transporting Electrons
High-energy electrons are released from NADH and FADH 2, and they move along
electron transport chains, like those used in photosynthesis. The electron transport
chains are on the inner membrane of the mitochondrion. As the high-energy electrons
are transported along the chains, some of their energy is captured. This energy is used
to pump hydrogen ions (from NADH and FADH 2) across the inner membrane, from the
matrix into the intermembrane space. Electron transport in a mitochondrion is shown
in Figure below.
Electron-transport chains on the inner membrane of the mitochondrion carry out the last stage of cellular respiration.
Making ATP
The pumping of hydrogen ions across the inner membrane creates a greater
concentration of the ions in the intermembrane space than in the matrix. This
chemiosmotic gradient causes the ions to flow back across the membrane into the
matrix, where their concentration is lower. ATP synthase acts as a channel protein,
helping the hydrogen ions cross the membrane. It also acts as an enzyme, forming ATP
from ADP and inorganic phosphate. After passing through the electron-transport chain,
the “spent” electrons combine with oxygen to form water. This is why oxygen is needed;
in the absence of oxygen, this process cannot occur.
Lesson Summary
Cellular respiration uses energy in glucose to make ATP. Aerobic (“oxygen-using”)
respiration occurs in three stages: glycolysis, the Krebs cycle, and electron transport.
In glycolysis, glucose is split into two molecules of pyruvate. This results in a net gain of
two ATP molecules.
Life first evolved in the absence of oxygen, and glycolysis does not require oxygen.
Therefore, glycolysis was probably the earliest way of making ATP from glucose.
The Krebs cycle and electron transport occur in the mitochondria. The Krebs cycle takes
place in the matrix, and electron transport takes place on the inner membrane.
During the Krebs cycle, pyruvate undergoes a series of reactions to produce two more
molecules of ATP and also several molecules of NADH and FADH2.
During electron transport, energy from NADH and FADH2 is used to make many more
molecules of ATP.
Recall
1. List the stages of aerobic respiration in the order in which they occur.
2. Describe what happens during glycolysis. How many ATP molecules are gained
during this stage?
6. What is the maximum number of ATP molecules that can be produced during the
electron transport stage of aerobic respiration?
Apply Concepts
7. When you exhale onto a cold window pane, water vapor in your breath condenses on
the glass. Where does the water vapor come from?
8. Assume that a new species of organism has been discovered. Scientists have
observed its cells under a microscope and determined that they lack mitochondria.
What type of cellular respiration would you predict that the new species uses? Explain
your prediction.
Think Critically
9. Why do scientists think that glycolysis evolved before the other stages of cellular
respiration?
10. Explain why two turns of the Krebs cycle are needed for each molecule of glucose.
Points to Consider
The last two stages of aerobic respiration require oxygen. However, not all organisms
live in places where there is a plentiful supply of oxygen.
How do you think organisms get energy from glucose to make ATP if they cannot use
oxygen?
Do they just use glycolysis, which produces only two ATP molecules? Or do you think
there might be other steps involved?
Lesson Objectives
Define fermentation.
Vocabulary
alcoholic fermentation
fermentation
Introduction
Today, most living things use oxygen to make ATP from glucose. However, many living
things can also make ATP without oxygen. This is true of some plants and fungi and
also of many bacteria. These organisms use aerobic respiration when oxygen is
present, but when oxygen is in short supply, they use anaerobic respiration instead.
Certain bacteria can only use anaerobic respiration. In fact, they may not be able to
survive at all in the presence of oxygen.
Fermentation
An important way of making ATP without oxygen is called fermentation. It involves
glycolysis but not the other two stages of aerobic respiration. Many bacteria and yeasts
carry out fermentation. People use these organisms to make yogurt, bread, wine, and
biofuels. Human muscle cells also use fermentation. This occurs when muscle cells
cannot get oxygen fast enough to meet their energy needs through aerobic respiration.
There are two types of fermentation: lactic acid fermentation and alcoholic fermentation.
Both types of fermentation are described below. You can also watch animations of both
types at this link: http://www.cst.cmich.edu/users/schul1te/animations/fermentation.swf.
Lactic acid fermentation produces lactic acid and NAD +. The NAD+ cycles back to allow glycolysis to continue so more ATP is
made. Each circle represents a carbon atom.
Did you ever run a race and notice that your muscles feel tired and sore afterward? This
is because your muscle cells used lactic acid fermentation for energy. This causes lactic
acid to build up in the muscles. It is the buildup of lactic acid that makes the muscles
feel tired and sore.
Alcoholic Fermentation
In alcoholic fermentation, pyruvic acid changes to alcohol and carbon dioxide. This is
shown in Figure below. NAD+ also forms from NADH, allowing glycolysis to continue
making ATP. This type of fermentation is carried out by yeasts and some bacteria. It is
used to make bread, wine, and biofuels.
Alcoholic fermentation produces ethanol and NAD+. The NAD+ allows glycolysis to continue making ATP.
Have your parents ever put corn in the gas tank of their car? They did if they used gas
containing ethanol. Ethanol is produced by alcoholic fermentation of the glucose in corn
or other plants. This type of fermentation also explains why bread dough rises. Yeasts
in bread dough use alcoholic fermentation and produce carbon dioxide gas. The gas
forms bubbles in the dough, which cause the dough to expand. The bubbles also leave
small holes in the bread after it bakes, making the bread light and fluffy. Do you see the
small holes in the slice of bread in Figure below?
The small holes in bread are formed by bubbles of carbon dioxide gas. The gas was produced by alcoholic fermentation carried
out by yeast.
The amount of energy produced by aerobic respiration may explain why aerobic
organisms came to dominate life on Earth. It may also explain how organisms were able
to become multicellular and increase in size.
E. coli bacteria are anaerobic bacteria that live in the human digestive tract.
Another advantage of anaerobic respiration is its speed. It produces ATP very quickly.
For example, it lets your muscles get the energy they need for short bursts of intense
activity (see Figure below). Aerobic respiration, on the other hand, produces ATP more
slowly.
The muscles of these hurdlers need to use anaerobic respiration for energy. It gives them the energy they need for the short-term,
intense activity of this sport.
Lesson Summary
Fermentation is a way of making ATP from glucose without oxygen. There are two types
of fermentation: lactic acid fermentation and alcoholic fermentation.
Lactic acid fermentation changes pyruvic acid to lactic acid and forms NAD+. The
NAD+ allows glycolysis to continue so it can make more ATP.
Alcohol fermentation changes pyruvic acid to ethanol and carbon dioxide and forms
NAD+. Again, the NAD+ allows glycolysis to keep making ATP.
Aerobic respiration produces much more ATP than anaerobic respiration. However,
anaerobic respiration occurs more quickly.