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    6 Laboratory Safety

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    Nipun Shamika
    1. Laboratory safety involves administrative, personal, procedural, and engineering controls to minimize risks when working with hazardous biological agents. 2. Key aspects of safety include proper training, use of personal protective equipment, biosafety cabinets, waste disposal procedures, and routine equipment maintenance. 3. Organisms are classified into hazard groups based on their risk level to help determine the containment measures needed like biosafety level to safely handle different biological agents.

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    6 Laboratory Safety

    Uploaded by

    Nipun Shamika
    69 views17 pages
    1. Laboratory safety involves administrative, personal, procedural, and engineering controls to minimize risks when working with hazardous biological agents. 2. Key aspects of safety include proper training, use of personal protective equipment, biosafety cabinets, waste disposal procedures, and routine equipment maintenance. 3. Organisms are classified into hazard groups based on their risk level to help determine the containment measures needed like biosafety level to safely handle different biological agents.

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    1. Laboratory safety involves administrative, personal, procedural, and engineering controls to minimize risks when working with hazardous biological agents. 2. Key aspects of safety include proper training, use of personal protective equipment, biosafety cabinets, waste disposal procedures, and routine equipment maintenance. 3. Organisms are classified into hazard groups based on their risk level to help determine the containment measures needed like biosafety level to safely handle different biological agents.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    69 views17 pages

    6 Laboratory Safety

    Uploaded by

    Nipun Shamika
    1. Laboratory safety involves administrative, personal, procedural, and engineering controls to minimize risks when working with hazardous biological agents. 2. Key aspects of safety include proper training, use of personal protective equipment, biosafety cabinets, waste disposal procedures, and routine equipment maintenance. 3. Organisms are classified into hazard groups based on their risk level to help determine the containment measures needed like biosafety level to safely handle different biological agents.

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    Download as pdf or txt
    of 17

    Laboratory Safety

    Dr Nayomi Danthanarayana
    Consultant Virologist
    TH Karapitiya
    Working with Risks

    • Smallpox How  smallpox  claimed  its  


    final  victim
    • Only infectious disease to be
    eradicated
    The  Smallpox  death  that  locked  
    • Global vaccination – successful down  Birmingham  could  have  
    been  avoided
    • Last natural case – 1977 in Somalia

    • Last laboratory acquired infection


    Birmingham UK

    • Medical photographer died

    • Virus escaped through the duct system


    Components of Laboratory Safety

    Safety
    Control
    Levels

    Classification of
    Electrical
    Safety
    organisms on 1. Personal level safety
    Hazardous level
    measures
    Laboratory
    Safety
    2. Safety equipments
    Use of
    Maintenance
    devices to
    of
    ensure 3. Laboratory structure
    equipments
    safety

    Routine
    procedures
    to ensure
    safety
    Safety control levels

    1. Administrative control

    2. Personal control

    3. Work practice or Procedure control

    4. Engineering control
    Administrative Control
    This has to be planed by the Laboratory In-Charge

    • Safety manual (with lab guidelines and policies)

    • Training (regularly, fire evacuation)

    • Occupational health measures (Vaccination, Records,


    post exposure prophylaxis, ICU to supervise)

    • Safety audits (regularly)

    • Inspection
    Personal Control
    Laboratory staff is responsible for the correct practice

    • PPE - gloves, masks, goggles, gowns, lab coats

    • Adequate supply

    • Train to choose appropriate PPE

    • Instructions in the safety manual

    • Supervision
    Work practices or procedure control

    Ensures safety when performing a test or using an equipment


    • Standard precautions
    • Work instructions
    • Access to equipment
    • Pre-work checklists
    • Good housekeeping
    • Lab space and equipments
    • Storage and labeling
    • Decontamination
    • Waste segregation and disposal
    Engineering Control
    Items or equipment used to reduce exposure to harmful
    substances

    • Closed systems

    • BSC

    • Safety carriers

    • Barrier tips

    • Emergency wash areas


    Hazard Group Organisms
    • 4 groups

    • Classification based on

    Organism pathogenic to man?

    Hazard to workers?

    Transmissible to community?

    Effective prophylaxis or treatment available?


    Hazard Groups
    1. Hazard Group 1 - Biological agent unlikely to cause human disease -
    Risk Negligible

    2. Hazard Group 2 - Biological agent, can cause human disease, may be


    a hazard to employees, unlikely to spread to community, treatment
    available Eg: Legionella - Risk Low

    3. Hazard Group 3 - Biological agent, can cause severe human disease,


    serious hazard to employees, may spread to community, but treatment
    available to prevent or control the onset of disease Eg: - Risk medium

    4. Hazard Group 4 - Biological agent, can cause severe human disease,


    serious hazard to employees, likely to spread to community, no
    treatment available to prevent or control the onset of disease Eg: Ebola
    - Risk High
    Containment levels
    Use of devices to ensure
    safety
    • Biological safety cabinets

    1. Class I - Operator and environment protection. No


    product protection

    2. Class II - Operator, environment and product


    protection

    3. Class III - High level Operator, environment and


    product protection
    • PPE

    • Air current control - avoid sudden movements, minimise


    opening and closing of door and windows, wait until flow
    is stabilised, Should not obstruct the front grill, flames are
    not allowed

    • Good laboratory practices - minimal equipments,


    decontamination before and after use, minimise spills,
    decontaminate any spill, UV lamp when indicated

    • Periodic checks - UV lamp, air flow, HEPA filters


    Routine Procedures to
    Ensure Safety

    • Hand washing

    • Sharp disposal

    • Waste disposal - colour code for waste segregation


    Electrical safety

    • Quality of equipments

    • Positioning and installation

    • Operation
    Maintenance of laboratory
    equipments
    • SOPs - operation, calibration, validation

    • Maintenance schedules with check lists with responsibility

    • Log book

    • Serviced as recommended

    • Repairs by qualified persons

    • Stock of essential spare parts


    Laboratory safety in summery

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