Activity 14 – Biochemical Engineering

A. Dixon Plot
1. The enzyme inhibition can occur by
B. Woolf-Augusteinssion-Hofstee plot
A. Reversible inhibitors
C. Eadie-Scatchard Plot
B. Irreversible inhibitors
D. Haes-Wolf plot
C. Both (a) and (b)
8. A noncompetitive inhibitor of an enzyme-
D. None of these
catalyzed reaction
2. In a Lineweaver-Burk Plot, competitive inhibitor
A. Increase KM and increase Vmax
shows which of the following effect?
B. Increase KM and reduces Vmax
A. It moves the entire curve to right
C. Reduces KM and increases Vmax
B. It moves the entire curve to left
D. Reduces KM and reduces Vmax
C. It changes the x-intercept
9. If liquid density and viscosity remains constant,
D. It has no effect on the slope
then the Reynolds number in a stirred tank
3. Which of the statement is true for continuous
reactor will vary with the
reactor at steady state?
A. Impeller diameter
A. The rates of biomass, substrate and product
B. Square rot of the impeller diameter
concentrations are zero
C. Square of the impeller diameter
B. Biomass, substrate and product
D. Cube of the impeller diameter
concentrations are zero
10. The mechanical means of accomplishing
C. Biomass, substrate and product
sterilization of fermentation media/equipment
concentrations do not change with time
is
D. Biomass, substrate and product
A. Ultrasonic
concentrations change with time
B. Radiation
4. An enzyme and a reactant molecule maintain
C. Chemical agents
relationship as
D. All of these
A. A temporary association
11. Sterilization can be carried out by
B. An association stabilized by a covalent bond
A. Heat
C. One in which the enzyme is changed
B. Radiation
permanently
C. Chemical agents
D. Non complementary binding
D. All of these
5. The plot commonly used for determining the
12. The collection efficiency by the inertial
value of Vmax is
impaction mechanism is the function of
A. Lineweaver Burk plot
A. Stokes and Reynolds Number
B. Langmuir Plot
B. Stokes and Schmidt Number
C. Eadie Hofstee Plot
C. Grashoff and Reynolds Number
D. All of these
D. Stokes and Grashoff Number
6. A classical uncompetitve inhibitor is a
13. Non-competitve inhibitor of an enzyme
compound that binds
catalyzed reaction
A. Reversibly to the enzyme substrate complex
A. Decreases Vmax
yielding an inactive ESI complex
B. Binds to Michaelis complex (ES)
B. Irreversibly to the enzyme substrate
C. Both (a) and (b)
complex yielding an inactive ESI complex
D. Can actually increase reaction velocity in
C. Reversibly to the enzyme substrate complex
rare cases
yielding an active ESI complex
14. The highest temperature which appears to be
D. Irreversibly to the enzyme substrate
feasible for batch sterilization is
complex yielding an active ESI complex
A. 121 deg C
7. The reciprocal equation for non competitive
B. 100 deg C
inhibition can be arrange to the equation for
C. 105 deg C
the
 Activity 14 – Biochemical Engineering
D. 130 deg C A. Using baffles
15. Gas hold up, characterizing the hydrodynamics B. Using diffusion ring with turbines
in a fermenter, mainly depends on the C. Both (1) and (2)
A. Superficial gas velocity D. None of these
B. Power consumption 23. The relationship between Keq, Km and Vmax is
C. Both (a) and (b) known as
D. Gas concentration A. Haldane equation
16. The collection efficiency by impaction increases B. Michaelis Menten equation
with C. Numerical solution approach
A. Increasing particle diameter D. Gibbs- Helmholtz equation
B. Increasing air flow velocity 24. Linear inhibition is sometimes called as
C. Both (a) and (b) A. Complete inhibition
D. Increasing particle size B. Incomplete inhibition
17. The value of K C. Partial inhibition
A. Increases with increase in air velocity D. Mixed inhibition
B. Decreases with increase in air velocity 25. Which of the following statements is true for
C. Increases to an optimum with increase in air enzymatically catalyzed reaction?
velocity A. The activation energy of the reaction is
D. None of these lowered so that a larger proportion of the
18. Which of the following is most likely to cause an substrate qualifies to overcome it.
increase in the rate of oxygen transfer into a B. Additional substrate molecules are
particular aerated fermentation system? energized to overcome the activation
A. Addition of antifoams energy of the reaction
B. Increase in temperature C. The activation energy of the reaction is
C. Increase in stirrer speed increased, thus decreasing the likelihood
D. Both (1) and (2) that any substrate molecules will overcome
19. The destruction of microorganisms by steam it
may be describe as D. The activation energy of the reaction is
A. First order chemical reaction lowered so that a fewer substrate
B. Zero order chemical reaction molecules can overcome it
C. Second order chemical reaction 26. The overall del factor may be represented as
D. None of these A. Δoverall = Δheating + Δholding – Δcooling
20. The mechanism of air filtration in fibrous filter is B. Δoverall = Δheating + Δholding + Δcooling
A. Electrostatic attraction C. Δoverall = Δheating - Δholding + Δcooling
B. Impaction D. Δoverall = Δheating - Δholding – Δcooling
C. Interception 27. The active site of n enzyme remains
D. All of these A. At the center of globular proteins
21. If the packing density is defines as the volume B. Rigid and does not change shape
of the fiber unit volume of filter bed, the C. Complementary to the rest of the molecule
velocity within the filter void space is equal to D. None of the above
(where V is velocity in the fluid void space and 28. The deviation from ideal plug flow due to axial
Vo is undisturbed fluid velocity) mixing can be describes by the
A. V=Vo/(1-α) A. Dispersion model
B. V-Vo=(1-α) B. Langmuir model
C. V=Vo(1-α) C. Friedlander model
D. V=Vo/(1+α) D. Pasceri Model
22. Swirling and vortex formation can be prevented 29. The heated medium passes through a holding
by section, which is usually maintained in
 Activity 14 – Biochemical Engineering
A. Adiabatic conditons
B. Isothermal conditions
C. Isotropic conditons
D. Isobaric conditions
30. The collection efficiency by diffusion increases
with
A. Decreasing particle size
B. Decreasing air velocity
C. Both (1) and (2)
D. Increasing particle size
31.