FLOWCHART

Attachment 1.

Determination of moisture content (AOAC 1970)

Weigh the sample as much as 1-2 g in a bottle that has been known to weigh scales

Then dry it in the oven at 100-C 105o during 3-5 hours depending on the material.

Then cooled in exilator and

weighed

This treatment is done until a

Reheat in oven 30 minutes constant weight is achieved.
longer

chill in exilator and weighed

Weight reduction is the abundance of water in the material

Attachment 2.

Determination Of Total Ash

Prepare a cup of ignorance, then Sear in Tanu

Allow to cool in the desiccator and weigh

Weigh as much as 3-5 g sample in the Cup

then put in the furnace ignorance, burned ash can be gray

ignorance is done in 2 stages: the first at a temperature of around 400 oC and second at
550oC

Allow to cool in the desiccator, then weigh

Note : before entering the furnace, used to fuel existing samples in vials of a gas burner
until the smoke is discharged
Attachment 3.

Procedure analysis of Nitrogen Total/Total Protein micro-

Kjeldahl Method

Weigh samples already in the puree as much as 0.2 grams

Enter the Kjeldahl pumpkin.

Add 0.7 grams of catalyst N

then add 4 ml of concentrated H2SO4.

Destruction in the cupboards of acid

then cool then add 10 ml aquadest.

Then in the distillation by adding 20 ml of NaOH – Tio

distillate in capacity use H3BO3 4%

distillate in capacity use H3BO3 4%

Run the distillate until volume distillate reach 60 ml

After the volume reached 60 ml stop distillation and then distillate in tit ration using a
standard solution 0.02 N HCl to the end point tit-ration

Record the volume of tit-ration are retrieved and then calculate the protein using the
formula.
Attachment 4.

The Procedure Analysis Method Of The Soxhlet Fat

Grab a fat pumpkin size corresponds to a Soxhlet extraction tool to be used

Dried in an oven

Allow to cool in the desiccator and weigh

Weigh 5 g of the sample in the form of flour in a sieve either directly, as appropriate to
its size, then cover with a cotton-wool fat-free.

Place the wrapped or filter paper containing the sample in a Soxhlet extraction tool

Then install the condenser tool on it, and a fat pumpkin below

Pour the solvent diethyl ether or petroleum ether into the FAT to taste the pumpkin, in
accordance with the size of the Soxhlet used

Do re-flux during minimum 5 hours until the solvent that fall back to clear colored fat
pumpkin

Solvent distillation in fat pumpkin, the capacity of solvent. Next pumpkin contains a fat
extraction results in a heated oven at 105oC

After dried up weight anyway and let cool in the desiccator, weigh the flask with the fat
can be calculated.
Attachment 5.

Coarse Fiber Analysis Procedure

Puree the ingredients until it can sift, the material must be free from grease or oil

Weigh ingredients 1gr, Erlenmeyer entries 250 ml

Add 200 ml of H2SO4 1.25%, heat in a water-bath temperature of 100C for 30 minutes
while

Then filter with filter paper and then wash with hot water until neutral (test with litmus
Paper)

Transfer the residue quantitatively into a 250 ml Erlenmeyer, and then the rest in the
wash with a solution of Na-OH 1.25% as much as 200 ml

Heat in a water bath temperature of 100oC for 30 minutes while in stir,

Filter by using filter paper already in the known reply constant weight (a)

Wash the residue with using ethanol 96% 15 ml

Wash using hot water until neutral (test with litmus paper)

Residue in filter paper and then in the oven at a temperature of 100oC to severe constant,

Weigh the residue in filter paper-me constant (b)

Attachment 6.
The procedure Analysis method of Flavonoid Spectrometer,
Worotikan In J, 2007

Weigh the sample 5 gr, dissolve in 100 ml of ethanol

Saring atau centrifuge larutan

Ambil 1 ml larutan jernih,tambahkan 3 ml larutan AlCl3 5 %

Tambahkan aquadest hingga volume 10 ml

Baca absorbansinya menggunakan spektrofotometer dengan panjang gelombang 420 nm

Buat kurva standarnya menggunakan Quercetein

Attachment 7.

Prosedur Analisa Phenol Metode Spektrofotometry

, JB. Harboune 1987

Timbang 5 gram sampel yang telah di haluskan ke dalam erelenmayer 100 ml.

Encerkan dengan aquadest sampai volume 100 ml menggunakan labu ukur.

Larutan disaring/centrifuge hingga diperoleh lrutan/filtrate jernih.

Ambil 1 ml larutan/filtrate jernih ke dalam tabung reaksi kemudian tambahkan 0,5 ml

follin denis ( follin 1:1 ),kemudian tambahkan 1 ml larutan Na2CO3 jenuh kemudian
diamkan selama 10 menit.

Tambahkan aquadest sampai volume 10 ml, kemudian vortex larutan hingga homogeny.

Baca Absorbasi sampel dengan menggunakan spektrofotometer dengan panjang

gelombang 730 nm.

Catat data yang diperoleh kemudian hitung dengan menggunakan kurva standar phenol.

Buat kurva standar phenol.

Attachment 9.

Kuesioner Uji Organeleptik Jeli Campuran Kersen Dan Kurma

KUESIONER

Nama :

NRP :

Tanggal pengujian :

Jenis sampel : Jeli campuran

Jenis pengujian : Aroma, warna, tekstur, dan rasa

Diahadapan saudara terdapat 3 sampel jeli campuran kersen dan kurma, saudara
diminta untuk memberikan penelitian kesukaan terhadap ketiga sampel tersebut, penelian
terhadap kesukaan terdiri dari angka 1-10 yang memiliki arti sangat tidak suka hingga suka.
Saudara diminta memberikan nilai sesuai dengan tingkat kesukaan saudara pada setiap
sampel.

Kolom penilaian AROMA :

Sampel Jeli 1 Jeli 2 Jeli 3

Nilai
Komentar : ...............................................................................................................................

Kolom penilaian WARNA :

Sampel Jeli 1 Jeli 2 Jeli 3

Nilai
komentar : ...............................................................................................................................

Kolom penilaian TEKSTUR :

Sampel Jeli 1 Jeli 2 Jeli 3

Nilai
Komentar : ...............................................................................................................................

Kolom penilaian RASA :

Sampel Jeli 1 Jeli 2 Jeli 3

Nilai

Komentar : ...............................................................................................................................