Meselson and Stahl's Experiment
Meselson and Stahl's Experiment
Meselson and Stahl's Experiment
15S
Student
Procedure
Complete the interactive tutorial associated with the activity, and then complete this worksheet.
Matthew Meselson and Frank Stahl worked at the California Institute of Technology. In 1958 they
grew bacteria in growth medium containing ammonium ions (NH4+) as the source of nitrogen. The
type of DNA made by the cells depended on the type of nitrogen present in the bacterias growth
medium. They used two isotopes of nitrogen 14N and 15N. 14N is the common, light form (isotope)
of nitrogen. 15N is the heavier form. They then extracted DNA from the bacterial cells and centrifuged
the resulting solution to isolate the DNA. The DNA made with 14N and the DNA made with 15N
accumulated at different levels in the centrifuged solutions, according to the DNAs density.
Questions
Q1 In each of the label boxes in Figure 1 below, fill in the number to show the type of nitrogen present in
each band.
Q2 In each magnified circle in Figure 1, colour in the sections of DNA molecule found in each band to show
14
the type of DNA in each band. Use your own colour code, selecting one colour for DNA made with N
15
and another colour for DNA made with N. You will need a colour for medium DNA later, so red
(heavy), blue (light) and purple (medium DNA) would work.
Q3 How did Meselson and Stahl produce bacterial cells containing only DNA
15
made with N?
Bacteria containing DNA made with 15N were allowed to divide once in a solution
containing only 14N. Any new DNA made would contain 14N. After a single
replication the DNA was extracted and centrifuged. Figure 2 DNA found after
first replication.
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A2.15S
iii Draw bands on the centrifuge tubes in Figure 5 to show the DNA present after the first and second
dispersive replications. Assume all the DNA has replicated after each cell division.
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b i In Figure 6 showing the semi-conservative theory of replication colour in the DNA and DNA
nucleotides using your colour code from question 2.
ii Now draw in the four DNA molecules produced when the DNA replicates for the second time,
according to the semi-conservative theory. This is where new DNA molecules have one strand of the
original (15N) DNA and one strand of the (14N) DNA. This is theory 2 in the interactive tutorial. Use
your colour code from question 2.
iii Draw bands on the centrifuge tubes in Figure 7 to show the DNA present after each replication.
Assume all the DNA has replicated after each cell division.
Q7 Meselson and Stahl found equal amounts of light and intermediate density (medium) DNA present after
two DNA replications. Explain which of these three theories for DNA replication is supported by this
evidence and which is refuted. Use a separate sheet of paper if you need more space for your answer.
Salters-Nuffield Advanced Biology, Pearson Education Ltd 2008. University of York Science Education Group.
This sheet may have been altered from the original. 3 of 3