Anil Kumar Shukla, Ph.

D
Laboratory of Protein Dynamics and Signaling 21112 Archstone Way, Apt. 203
Bldg 560/12-17, CCR, NCI-Frederick, NIH, MD, USA 21702-1201 Germantown, MD, USA 20876
+1(301)846-6023(Office), +1(240 )586-2105 (Mobile)
[email protected], [email protected]

Education
2008- 2012: PhD at Department of Biosciences and bioengineering, Indian Institute of Technology Guwahati, India
(CGPA course work: 9.5/10).
Thesis Title: Targeting redox metabolism of Leishmania parasite for potential chemotherapy of
Leishmaniasis.

2006-2008: Master of Science at Department of Biochemistry, Banaras Hindu University, Varanasi, India. (CGPA:
8.93/10).
st
(BHU Gold Medal for 1 Rank).

2001-2004: Bachelor of Science in Zoology, Botany & Chemistry at Lucknow University, Lucknow, India. (% Marks:
67.67%)

1999-2001: Higher Secondary Examination (XII), U. P. Board. (% Marks: 74%)

1997-1999: High School Examination (X), U. P. Board. (% Marks: 80.67%)

(Gold Medal for 1st Rank in School).

Research
2012-Present: Postdoctoral Fellow at National Cancer Institute (NCI), National Institutes of Health, Frederick, MD, USA.

Project: Characterization of the process of centriole disengagement at molecular level: Centrioles are microtubule-
based structures that organize a proteinaceous matrix around them, thus, forming a centrosome. There are only two
centrosomes in a cell due to stringent control over centriole duplication. Centrosome over-duplication promotes
mitotic abnormalities, invasion, ciliopathies, and tumorigenesis. The long-term goal of the project is to understand
the mechanism(s) that coordinate centriole cycle and cell cycle events to prevent centriole overduplication. We
employ fixed-cell and correlative live/electron microscopy analysis and other molecular and biochemical
techniques to elucidate the structural and molecular changes at the centrosome during centriole duplication.

2008-2012: PhD student at Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, India.

Project: Targeting redox metabolism of Leishmania parasite for potential chemotherapy of Leishmaniasis: I have done
extensive studies on Trypanothione reductase (TryR), a key enzyme of thiol metabolism of Leishmania sp. and
identified several candidate drugs of natural and commercial source against Leishmaniasis. Subsequently, I
characterized the biochemical and folding parameters of TryR. Furthermore, I have deciphered the molecular
mechanisms underlying anti-leishmanial activity of a few anti-tumor agents and iridoid glucosides from Nyctanthes
arbortristis, an Indian medicinal plant. I have also worked on delivery of these compounds to macrophages using
polymeric nanoparticles.

Technical Skills
1. Imaging: Live/fixed cell widefield, confocal and super resolution microscopy (SIM/STORM/STED), Correlative
light and Electron microscopy (CLEM), MATLAB.

2. Cell Culture: Maintenance of different types of mammalian cell lines, Cell cycle analysis, Cytotoxicity studies,
Oxidative stress studies, Apoptosis analysis, drug targeting.
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3. Proteomics & Molecular Biology: Protein expression, purification & characterization, Western blots, Silver
staining, Immunoprecipitation, centrosome isolation, MASS spectrometry, SILAC, Enzyme kinetics, determination
of folding-unfolding parameters, Gene cloning and expression.

4. Instrumental Skills: Fluorescence activated cell sorter (FACS), Scanning Electron Microscope (SEM),
Fluorescence, UV-Visible and Infrared Spectrophotometer, Exposure to Atomic Force Microscope (AFM), Nuclear
Magnetic Resonance (NMR), X-ray diffractometer (XRD), High Pressure Liquid Chromatography (HPLC), GC
(Gas Chromatography) etc.

5. Computational Skills: ImageJ, Molecular modeling, Docking studies, Analysis of protein structures, Basics of
alignment, Computational analysis of protein-ligand binding.

Awards and Scholarships

1. Awarded prestigious and highly competitive Cancer, Genetic and Signaling fellowship (CGS) by NCI, National
Institutes of Health (NIH) to pursue postdoctoral career.

2. Selection and Funding by IIT Gandhinagar to attend Young Researcher Conclave-2011 at IIT Gandhinagar, India
during 27-28 December, 2011.

3. Selected for the prestigious meeting organized by Wellcome Trust and DBT India Alliance among top 30
researchers, 28-30 March, 2012

4. International Travel Fellowship grant by Department of Science and Technology, Govt. of India to attend EMBO
Meeting 2011 at Vienna, Austria during 10-13 September 2011.

5. Graduate Aptitude Test in Engineering (GATE-2008), a National Level Examination (All India Rank-06;
Percentile: 99.96).

6. Qualified a national level examination conducted by Council for Scientific and Industrial research, Govt. of India
for lectureship and research fellowship (CSIR-NET): JRF 2008, LS 2007

7. Call for Shyama Prasad Mukherjee (SPM) fellowship, 2008.

8. Gold Medal for 1st Rank in Master of Science (M. Sc.) from Banaras Hindu University.

9. Gold Medal for 1st Rank in school in High School.

Publications
1. Thirthagiri, E., Klarmann, K.D., Shukla A.K., Southon, E., Biswas, K., Martin, B.K., et al. BRCA2 minor transcript
lacking exons4-7 supports viability in mice and may account for survival of humans with a pathogenic biallelic
mutation. Human Molecular Genetics, DOI: 10.1093/hmg/ddw066 (2016).

2. Shukla, A., Kong, D., Sharma, M., Magidson V., and Loncarek, J. Plk1 relieves centriole block to reduplication by
promoting daughter centriole maturation. Nature Communications 6:8077 doi: 10.1038/ncomms9077 (2015).

3. Kong, D., Farmer, V., Shukla, A., James, J., Gruskin, R., Kiriyama, S., and Loncarek, J. Centriole maturation
requires regulated Plk1 activity during two consecutive cell cycles. Journal of Cell Biology 206, 855-865 (2014).

4. Khanna, S., Shukla, A.K., Goyal, A., Moholkar, V.S. Alcoholic Biofuels Production from Biodiesel Derived
Glycerol by Clostridium pasteurianum Whole Cells Immobilized on Silica, Waste and Biomass Valorization 5 789-
798 (2014).

5. Kim, T.S., Park, J.E., Shukla, A., Choi, S., Murugan, R.N., Lee, J.H., Ahn, M., Rhee, K., Bang, J.K., Kim, B.Y., et
al. Hierarchical recruitment of Plk4 and regulation of centriole biogenesis by two centrosomal scaffolds, Cep192 and
Cep152. PNAS 110, 25 (2013).
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6. Shukla, A.K., Patra, S., Dubey, V,K. Nanospheres encapsulating antileishmanial drugs for their specific
macrophage targeting, reduced toxicity and deliberate intracellular release, Vector Borne and Zoonotic Diseases 12,
953-960 (2012).

7. Shukla, A.K., Patra, S., Dubey, V.K. Iridoid glucosides from Nyctanthes arbortristis result in increased Reactive
Oxygen Species (ROS) and cellular redox homeostasis imbalance in Leishmania parasite. European Journal of
Medicinal Chemistry 54, 49-58 (2012).

8. Sharma, N., Shukla, A.K., Das, M., Dubey, V.K. Evaluation of plumbagin and its derivative as potential modulator
of redox thiol metabolism of Leishmania parasite. Parasitology Research 110. 341-348 (2012).

9. Venkatesan, S.K., Saudagar, P., Shukla, A.K., Dubey, V.K. Screening natural products database for identification
of potential antileishmanial chemotherapeutic agents. Interdisciplinary Sciences: Computational Life Sciences, 3,
217-231(2011).

10. Shukla, A.K., Patra, S., Dubey, V.K. Evaluation of selected antitumor agents as subversive substrate and potential
inhibitor of trypanothione reductase: An alternative approach for chemotherapy of Leishmaniasis. Molecular and
Cellular Biochemistry 352, 261-70 (2011). (Research Highlight of the article published in Nature India)

11. Shukla, A.K., Patra, S., Dubey, V.K. Deciphering molecular mechanism underlying antileishmanial activity of
Nyctanthes arbortristis, an Indian medicinal plant. Journal of Ethnopharmacology, 134, 996-998 (2011).

12. Shukla, A.K., Patra, S., Dubey, V.K. Biophysical and Folding parameters of Trypanothione reductase from
Leishmania infantum. Applied Biochemistry and Biotechnology, 165, 13-23 (2011).

13. Shukla, A.K.*, Kannan, S.*, and Dubey, V.K. Molecular docking studies of selected tricyclic and quinone
derivatives on trypanothione reductase of Leishmania infantum. Journal of Computational Chemistry, 31, 2463-
*
2472 (2010) ( Equal contribution) .(Research Highlight of the article published in Nature India)

14. Shukla, A.K., Singh, B.K., Patra, S., Dubey, V.K. Rational approaches for drug designing against leishmaniasis.
Applied Biochemistry and Biotechnology 160, 2208-2218 (2010).

15. Singh, A.N., Shukla, A.K., Jagannadham, M.V., Dubey, V.K. Purification of a novel cysteine protease, procerain B,
from Calotropis procera with distinct characteristics compared to procerain. Process Biochemistry, 45, 399-406
(2010).

16. Shukla, A.K., Bora, U., Dubey, V.K. Functional Adaptations in Fibroblast Growth Factor (FGFs) Family. Journal
of Proteins and Proteomics, 1, 11-13 (2009).

17. Rizvi, S.B., Shukla, A.K., Dubey, V.K. A Simple method based on multiple alignment and phylogeny to derive a
correlation between the protein fold and sequence via motif search. Interdisciplinary Sciences: Computational Life
Sciences, 1, 235-243 (2009).

Manuscript in Preparation

Shukla, A., Kong D., and Loncarek, J. Prophase: A point of no return for the loss of centriole block to reduplication.
(Manuscript in preparation)

Conferences/Seminars
1. Shukla, A.K., Kong, D., Sharma, N., Loncarek, J. Plk1 relieves centriole block to reduplication by promoting
daughter centriole maturation 2015 ASCB Meeting, San diego, CA, USA, Dec 12-16, 2015.(Poster)

2. Shukla, A.K., Loncarek, J. The loss of block to centriole reduplication without a loss of orthogonal orientation.
Centrosome Retreat, NCI-Frederick, MD, USA, Mar 26, 2015.(Poster)
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3. Shukla, A.K., Kong, D., Loncarek, J. The loss of block to centriole reduplication without a loss of orthogonal
orientation. ASCB Meeting Philadelphia, PA, USA, Dec 6-10, 2014. (Poster)

4. Shukla, A.K., Loncarek, J. The intrinsic block to centriole reduplication: a matter of mother-to-daughter centriole
distance. Centrosome Retreat, NCI-Frederick, MD, USA, Jun 26, 2014 (Oral).

5. Shukla, A.K., Loncarek, J. The intrinsic block to centriole reduplication: a matter of mother-to-daughter centriole
distance. CGS Seminar, NCI-Frederick, MD, USA, Jun 10, 2014 (Oral).

6. Shukla, A.K., Kong, D., Loncarek, J. Two step model for centriole disengagement: centriole distancing followed by
(facultative) centriole disorientation, 2014, NCI Retreat, ATRF, NCI-Frederick, USA. (Poster)

7. Shukla, A.K., Farmer, V., Kong, D., Loncarek, J. Centriole disengagement in the absence of microtubules. ASCB
Meeting New Orleans, LA, USA, Dec 13-18, 2013. (Poster)

8. Shukla, A.K., Kong, D., Loncarek, J. Centriole disengagement in the absence of microtubules, 2013 NIH Research
Festival, NIH Bethesda. (Poster)

9. Shukla, A.K., Kong, D., Loncarek, J. Centriole disengagement in the absence of microtubules, 2013 NIH LPDS
Retreat, NCI Frederick. (Poster)

10. Dubey, V.K., Shukla, A.K., Prakash, S. Current trends in anti-leishmanial drug discovery. International conference
on new horizons in biotechnology (BRSI). Trivandram, Nov. 21-24, 2011(Oral).

11. Shukla, A.K., Patra, S., Dubey, V.K. Redox metabolism of Leishmania parasite: Ideal target for drug development
against leishmaniasis. Internationl Journal of Infectious Disease, 16, 1, 34 (2012), Conference proceedings.

12. Shukla, A.K., Dubey, V.K. Subversive substrates of trypanothione reductase of Leishmania parasite: an alternative
approach for chemotherapy. Amino Acids, 41, S34-S34 (2011) Conference proceedings.
th
13. Shukla, A.K., Dubey, V.K. Targeting redox system of Leishmania sp. for drug development. 18 ISCB-2012 at
IASST Guwahati, India, Jan. 28-30, 2012. (Poster)

14. Shukla, A.K., Dubey, V.K. Teaching and Research in Leading Indian Institutes: Scope of Improvements. YRC-
2011 at IIT Gandhinagar, India, Dec. 27-28, 2011.

15. Shukla, A.K., Dubey, V.K. Synthesis of PEGylated Nanospheres encapsulating doxorubicin and mitomycin c for
their specific macrophage targeting, reduced toxicity and enhanced antileishmanial activity. EMBO meeting 2011 at
Vienna, Austria, Sept. 10-13, 2011. (Poster)

16. Bharadwaj, R., Shukla, A.K., Dubey, V.K. Synthesis of Chitosan-PEG coated gold nanoparticles entrapping
doxorubicin for evaluation of antileishmanial activity and targeted delivery to macrophages. 80th Annual Meeting of
SBCI, CIMAP, Lucknow, Nov. 12-15, 2011. (Poster)

17. Sharma, N., Shukla, A.K., Dubey, V.K. Evaluation of Plumbagin and its derivative as potential modulator of redox
thiol metabolism of Leishmania parasite World Congress on Biotechnology, Hyderabad, Mar. 21-23, 2011. (Poster)

18. Shukla, A.K., Dubey, V.K. Underlying the anti-leishmanial activity of Nyctanthes arbortristis, an Indian medicinal
th
plant. 4 ICDDR. Central Drug Research Institute, Lucknow. Feb. 17-21, 2010. (Poster)

19. Shukla, A.K., Dubey, V.K. Biophysical properties of TryR from Leishmania infantum: a step towards drug
development for Leishmaniasis. IncoFIBS-2010, NIT Rourkela, Oct. 1-3, 2010. (Poster)

20. Shukla, A.K., Venkatesan, S.K., Dubey V.K. Studies on Trypanothione Reductase from Leishmania infantum. 78th
Annual Meeting of SBCI held at National Centre for Cell Science and University of Pune, Oct. 30-Nov. 1, 2009.
(Poster)
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Professional Training
1. FAES High Resolution Microscopy Training Course: NIH, Bethesda, Maryland, USA (Oct 15th to Oct 17th
2014).
2. FAES Statistical Analysis Course: NIH, Bethesda, Maryland, USA (Jun 12th to 13th 2013)

Patents
1. Iridoid glucosides from Nyctanthes arbortristis as new class of inhibitor of Trypanothione reductase of leishmania
parasite. (306/KOL/2010)
2. A novel cysteine protease, procerain B, from Calotropis procera. (1346/KOL/2009).

Scientific / Teaching Service

1. 2012-2015: Training of three summer interns at NCI Frederick, NIH.
2. 2010: B. Tech 1st Year Modern Biology Course
3. 2011: B. Tech 3rd Year Bioinformatics Practical Course.
4. 2011: Operation of FESEM in Central Instrument Facility of the Institute.

Professional Society Memberships

1. Annual Member, American Society for Cell Biologists (ASCB).
2. Life Member, Society of Biological Chemists, India (SBCI).
3. Life Member, Indian Science Congress Association (ISCA).
4. Life Member, International Society of Infectious Diseases (ISID).
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References

1. Dr Jadranka Loncarek
(Postdoc Supervisor)
NIH Stadtman Investigator
Center for Cancer Research
National Cancer Institute
Building 560, Room 12-90
Frederick, MD, USA 21702.
E-mail: [email protected]
Phone: +1 301-846-1059

2. Prof. Vikash Kumar Dubey

(PhD Supervisor)
Professor
Department of Biosciences and Bioengeering
Indian Institute of Technology Guwahati
Guwahati, Assam, India-781039
E-mail: [email protected]
Phone: +91 361-2582203

3. Dr. Allan M. Weissman

Lab Chief, LPDS
Center for Cancer Research
National Cancer Institute
Building 560, Room 22-103
Frederick, MD 21702.
E-mail: [email protected]
Phone: +1 301-846-7540

4. Dr. Shyam K. Sharan

Deputy Program Director
Mouse Cancer Genetics Program
& Head, Genetics of Cancer Susceptibility Section
Center for Cancer Research
National Cancer Institute
Building 560, Room 32-31C
Frederick, MD 21702.
E-mail: [email protected]
Phone: +1 301-846-5140