COVER LETTER

Dear Sir,

I am writing for the postdoctoral position and would like to continue my research career
in your reputed laboratory. Presently, I am working as an Assistant Professor pursuing both
academic and research work in Department of Biotechnology, Era’s Lucknow Medical College
& Hospital, Era University, Lucknow, India.
I have completed my Ph.D. degree under the supervision of Dr. Md Arshad (Associate
Professor), Molecular Endocrinology Laboratory, Department of Zoology, University of
Lucknow, Lucknow, India in 2016. My area of research interest is related to cancer biology and
bone cell research.
I have rich experience of Bone and Cancer cell biology including bone marrow
progenitor cells culture, primary osteoblasts, chondrocytes cells culture and different types of
cancer cells culture and relevant cellular and molecular techniques. I carried out in vitro and in
vivo studies using various cellular and molecular techniques such as ELISA techniques,
Immunocytochemical, Histochemical, Flow cytometric analysis, DNA, RNA extraction and real-
time PCR techniques, Protein blotting, and so on. I have self-sufficient, excellent
communication, writing and data interpretation skills. I have the ability to work independently
and independent scientific research investigations.
Please look at the CV and let me know if you need some more information about your research
career and interest.

Regards,

Sahabjada, Ph.D.

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 Curriculum Vitae

Dr. Sahabjada
(Assistant Professor)
Department of Biotechnology,
Era’s Lucknow Medical College & Hospital,
Era University, Lucknow-226003, India
Phone: +91-9452688313; 8317010035
Email: [email protected]
Skype ID: sahabjada

Education

 February 2011- January 2016: PhD from Molecular Endocrinology Laboratory,

Department of Zoology, University of Lucknow, Lucknow, India.
 Supervisor: Dr. Md Arshad, Assistant Professor, Molecular Endocrinology Laboratory,
Department of Zoology, University of Lucknow, Lucknow, India.
 Thesis title: “Osteogenic Prospective of Indian Medicinal Plants: Cellular and Molecular
Study”
 2007: Mater of Science (M.Sc.) in Biotechnology from Department of Biotechnology,
Chhatrapati Shahu Ji Maharaj University, Kanpur, India.
 2003: B.Sc. in ZBC from Deen Dayal Upadhyay, Gorakhpur University, Gorakhpur,
India.

Research and Professional Experience

 Assistant Professor (Feb 2018 – Till date): Tissue Culture Lab, Department of
Biotechnology, Era’s Lucknow Medical College & Hospital, Era University, Lucknow,
India.
 Post Doctoral Fellow (August, 2017–Jan, 2018): Tissue Culture Lab, Department of
Biochemistry, Era’s Lucknow Medical College & Hospital, Era University, Lucknow,
India.
 Research Associate (February, 2016–December, 2016): Indian Council of Medical
Research (ICMR), New Delhi, India.

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  Senior Research Fellowship (December, 2013–January, 2016): Indian Council of
Medical Research (ICMR), New Delhi, India.
 Project Fellow (April, 2010–March, 2013): University Grant Commission (UGC), New
Delhi, India.
 Junior Research Fellow (August, 2009–March, 2010): Indian Council of Medical
Research (ICMR), New Delhi, India.
 Project Assistant Level-II (June, 2008–June, 2009): Council of Scientific Industrial
Research (CSIR), New Delhi, India.
 Qualified UGC-NET, December- 2008- conducted by CSIR, New Delhi, India.
 Qualified Graduate Aptitude Test in Engineering (GATE)- 2007 and 2010, conducted by
Indian Institute of Technology (IIT), India.

Research Summary

Present Research Work

Presently, I am working on anticancer activities of different herbal extracts and
phytocomponents on various cancer cell lines to detect the different apoptotic pathways and
signaling molecules involved in apoptosis cell death (in-vitro study). Moreover, my research
work is also focused on the antitumor activity of the herbal extracts and compounds in tumor
model of mice (in-vivo study).
Ph.D. work
The focal aim of my Ph.D. work was based on osteogenic prospective of Indian medicinal plants
using different cellular and molecular parameters in primary rat calvaria osteoblasts and bone
marrow progenitor cells. For this study, I examined phytochemical constituents of medicinal
plants extract using GC-MS technique. I used different biochemical and cytochemical parameters
including cell proliferation assay, alkaline phosphatase assay (ALP), collagenation,
mineralization, ROS generation, nuclear apoptosis, caspase-3/7 activity and cell cycle
progression in primary rat osteoblasts and human osteosarcoma MG63, SaOS2 cell lines.
Moreover, I carried out molecular study of different osteogenic genes expression such as Runx2,
osteocalcin and BMP-2 by real-time qPCR. I also studied on induction of osteoclasts from bone
marrow progenitor cells (BMCs) of mice using TRAP stain and immunofluorescence stain of
Cathepsin K expression. In addition, I isolated and cultured primary chondrocyte from cartilage

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region of rib, femur and tibia of rat. I also learned in vivo study on modulation of bone turn-over
in alloxan induced type I diabetic rat model and chondroprotective effect of selected medicinal
plant extracts on collagenase induced osteoarthritic rat model. Blood glucose measurements,
serum ALP, cholesterol, creatinine, glutamic oxaloacetate transaminase (GOT), glutamic
pyruvate transaminase (GPT) and triacyl glycerides were measured. Histology of pancreas,
uterus, ovary and microtomy of femur and tibia bones were also carried out.
Moreover, I also carried out anticancer activity of different phytochemicals, synthetic
drugs and nanomaterials on different cancer cell lines. For this, I evaluated cell cytotoxicity
assay, cellular apoptosis, ROS generation, mitochondrial membrane potential, cell cycle study by
flow cytometry, DNA fragmentation, cyctochrome-C release, western blot analysis of Bax, Bcl2,
p53 and immunocytochemical analysis of caspase3/7 activities. I have self-sufficient, excellent
communication, writing and data interpretation skills. I have the ability to work independently
and independent scientific research investigations.

Technical Proficiency

 Cell and tissue culture: Isolation and culture calvaria osteoblasts and chondrocyte cells
from rat model and induction of osteoclasts from bone marrow progenitor cells. Culture and
preservation of different osteoblast and different cancer cell lines viz. SaOS2, MG63.
 Animal handling: Rats/Mice
 In vitro studies: Evaluation of cell proliferation, cell cycle study, mineralization assay,
collagenation, alkaline phosphatase activity and anti-resorptive activity in bone cells.
Anticancer activity including nuclear apoptosis, MMP reduction, DNA fragmentation,
Annexin V/FITC, ROS generation and caspase 3 estimation of promising anticancer agents
in different cancer cell lines (HepG2, MCF7, PC3, A549, HeLa, A431, HEK293, KB, etc.).
 Molecular analysis: Isolation and estimation of genomic DNA/RNA, cDNA synthesis, PCR
amplification, Restriction digestion, carried out agarose and polyacrylamide gel
electrophoresis, quantitative real-time PCR of expression analysis of bone cells specific
genes and apoptotic markers of cancer cell lines. Estimation of pro-apototic and anti-apototic
markers of cancer cells and immunoblot analysis of different protein markers in bone cells
and cancer cells.
 In vivo osteogenic/toxicity studies: RBC lysis assay, LDH release assay, Blood glucose

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 measurements, serum ALP, cholesterol, creatinine, serum glutamic oxaloacetate
transaminase (SGOT), serum glutamic pyruvate transaminase (SGPT) and triacyl glycerides.
Histology of pancreas, uterus, ovary and microtomy of femur and tibia bones. Estimation of
biochemical markers of bone turn over including serum osteocalcin, estradiol and
progesterone. Estimation of antioxidant enzymes and bone mineral contents.
 Histochemistry and Immunocytochemistry: Histochemical and immunocytochemical
localization of osteoclasts markers in decalcified bone tissues.
 Microbial techniques: Isolation and identification of antibiotic sensitive bacteria,
maintenance of microbiological strains, plasmid isolation from microbial cells, cloning and
transformation.
 Proteomics: Isolation and estimation of proteins, PAGE-SDS & Native.
 Bioinformatics and computer skills: Knowledge of computational and statistical analysis of
biological systems by using softwares viz. SPSS-17, and GraphPad Prism 5, BLAST, Primer
design using NCBI and other web based bioinformatics tools.

Publications

First author/corresponding authors/equal contribution research articles

1. Siddiqui Sahabjada*, Ahmad R, Khan MA, Srivastava AN. Cytostatic and anti-tumor
activity of Ajwa date against human hepatic carcinoma HepG2 cells: SREP-18-31479R1;
Scientific Reports, 2018 (Under Minor Revision).
2. Siddiqui Sahabjada, Md Sultan Ahamad, Asif Jafri, Afzal M, Arshad M. Piperine triggers
apoptosis of human oral squamous carcinoma through cell cycle arrest and mitochondrial
oxidative stress. Nutrition and Cancer 2017; 20:1-9. IF-2.24, ISSN:0163-5581.
3. Siddiqui Sahabjada, Ahmad E, Gupta M, Rawat V, Shivnath N, Banerjee M, Khan MS,
Arshad M. Cissus quadrangularis Linn exerts dose dependent biphasic effects: osteogenic
and antiproliferative through modulating ROS, cell cycle and Runx2 gene expression in
primary rat osteoblasts. Cell proliferation 2015; 48, 443-454. IF-4.12, ISSN:1365-2184
4. Kaleem S*, Siddiqui Sahabjada*, Hussain A, Arshad M, Akhtar J, Rizvi A, Siddiqui HH.
Eupalitin induces apoptosis in prostate carcinoma cells through ROS generation and increase
of caspase-3 activity. Cell Biology International 2015; 40:196-203 IF-1.93 ISSN:1065-
6995 (*Equal contribution and Corresponding Author)

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5. Siddiqui Sahabjada and Arshad M. Osteogenic potential of Punica granatum through
matrix mineralization, cell cycle progression and Runx2 gene expression in primary rat
osteoblasts. DARU Journal of Pharmaceutical Sciences 2014; 22:72. IF-1.64, ISSN:2008-
2231.
6. Ahamad MS*, Siddiqui S*, Jafri A, Ahmad S, Afzal M, Arshad M. Induction of apoptosis
and antiproliferative activity of naringenin in human epidermoid carcinoma cell through
ROS generation and cell cycle arrest. PLoS ONE, 2014; 9(10): e110003. IF-3.23,
ISSN:1932-6203 (*Equal contribution).
7. Jigyasu AK, Siddiqui S*, Lohani M, Khan IA, Arshad M. Chemically synthesized CdSe
quantum dots inhibit growth of human lung carcinoma cells via ROS generation. EXCLI
Journal 2016; 15:54-63. IF-1.29, ISSN:1611-2156. (*Corresponding author).
Co-author research articles
1. Rais J, Jafri A, Siddiqui Sahabjada, Tripathi M, Arshad M. Phytochemicals in the
treatment of ovarian cancer. Frontiers in Bioscience (Elite Ed) 2017; 9:67-75. IF-2.48,
ISSN:1093-9946.
2. Ahmad U, Akhtar J, Singh SP, Ahmad FJ, Sahabjada Siddiqui. Silymarin nanoemulsion
against human hepatocellular carcinoma: development and optimization. Artificial Cells,
Nanomedicine and Biotechnology 2017; 14:1-11. IF-5.02, ISSN: 2169-1401.
3. Arshad M, Siddiqui Sahabjada, Ali D. In vitro anti-proliferative and apoptotic effects of
ethanolic extract of Cissus quadrangularis. Caryologia: International Journal of Cytology,
Cytosystematics and Cytogenetics 2016; 69:128-132. IF-0.74, ISSN:2165-5391.
4. Iram S, Khan S, Ansary AA, Arshad M, Siddiqui Sahabjada, Ahmad E, Khan RH, Khan
MS. Biogenic Terbium Oxide Nanoparticles as the Vanguard against Osteosarcoma.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2016; 168:123-
131. IF- 2.65, ISSN:1386-1425
5. Singh AK, Saxena G, Sahabjada, Arshad M. Synthesis, characterization and biological
evaluation of ruthenium flavanol complexes against breast cancer. Spectrochimica Acta
Part A: Molecular and Biomolecular Spectroscopy. 2017; 180:97-104. IF- 2.65,
ISSN:1386-1425
6. Rahman MA, Akhtar J, Sahabjada, Arshad M. Evaluation of cytotoxic potential and
apoptotic effect of a methanolic extract of Bauhinia racemosa Lam against a human cancer

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 cell line, HeLa. European Journal of Integrative Medicine 2016; 8:513-518. IF-0.84,
ISSN:1876-3820
7. Sheikh S, Siddiqui Sahabjada, Dhasmana A, Safia, Haque E, Kamil M, Lohani M, Arshad
M, Mir SS. Cissus quadrangularis Linn stem ethanolic extract liberates reactive oxygen
species (ROS) and induces mitochondria mediated apoptosis in KB cells. Pharmacogosy
Magzine 2015; 11:365-374. IF-1.26, ISSN:0976-4062.
8. Mishra N, Siddiqui Sahabjada, Arshad M, Kumar D. In vitro anticancer activities of
Schiff base and its lanthanum complex. Spectrochimica Acta Part A: Molecular and
Biomolecular Spectroscopy 2016; 155:146-154. IF-2.65, ISSN:1386-1425
9. Shanmugaprakash M, Jayashree C, Vinothkumar V, Siddiqui Sahabjada, Rawat V,
Arshad M. Biochemical characterization and antitumor activity of three phase partitioned L-
asparaginase from Capsicum annuum L. Separation and Purification Technology 2015;
142:258-267. IF-3.09, ISSN:1383-5866.
10. Usmani S, Hussain A, Farooqui AHA, Arshad M, Siddiqui Sahabjada, Ahmad M, Wahab
S. Anti-proliferative activity of crude extract and fractions obtained from Digera muricata
on HeLa cell lines of human cervix and A549 cell lines of human lung. Pharmacognosy
Journal, 2014; 6:32-38. IF-1.26, ISSN:0975-3575.
11. Iqbal B, Ghildiyal A, Sahabjada, Singh S, Arshad M, Mahdi AA, Tiwari S.
Antiproliferative and Apoptotic Effect of Curcumin and TRAIL (TNF related apoptosis
inducing ligand) in Chronic Myeloid Leukemic Cells. Journal of Clinical and Diagnostic
Research; 2016; 10:XC01-XC05. IF-0.3, ISSN:0973-709X.
12. Ahmad M, Sahabjada, Akhtar J, Hussain A, Badaruddeen, Arshad M, Mishra A.
Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3
cell line. EXCLI Journal 2017; 16:810-823. IF-1.29, ISSN:1611-2156. (Accepted).
13. Kirwen EM, Batra T, Karthikeyan C, Deora GS, Rathore V, Sahabjada, Arantes V,
Shivnath N, Chaurasia D, Arshad M, Trivedi P, Tiwari AK. 2,3-Diaryl-3H-imidazo[4,5-
b]pyridine derivatives as potential anticancer & anti-inflammatory agents. Acta
Pharmaceutica Sinica B 2017; 7:73-79. ISSN: 2211-3835.
14. Rahman MA, Sahabjada, Akhtar J. Evaluation of anticancer activity of Cordia dichotoma
leaves against a human prostate carcinoma cell line, PC3. Journal of Traditional and
Complementary Medicine, 2016; 7:1-7. ISSN:2225-4110.

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15. Usmani S, Ahmad M, Sahabjada, Hussain A, Arshad M, Ali M. Cellular oxidative stress
and antiproliferative effects of Cordia dichotoma (Linn.) seeds extract and their fractions on
human cervix epitheloid (HeLa) and human lung (A549) carcinoma cells. European
Journal of Integrative Medicine. 2018; 21:1-10. IF-0.84, ISSN:1876-3820.
16. Iqbal B, Ghildiyal A, Singh S, Siddiqui Sahabjada, Kumari P, Arshad M, Mahdi AA.
Combinatorial effect of curcumin and tumor necrosis factor-α-related apoptosis-inducing
ligand in induction of apoptosis via inhibition of nuclear factor kappa activity and
enhancement of caspase-3 activity in chronic myeloid cells: An in vitro study. Journal of
Cancer Research and Therapeutics, 2018; ISSN: 1998-4138; IF-0.84, (Accepted).
17. Trivedi A, Ahmad R, Sahabjada, Misra A. Effect of alkaline pH on cytotoxicity profile of
Azadirachta indica ethanolic extract against human breast cancer cell line MDA-MB-23
European Journal of Integrative Medicine, 2018; ISSN:1876-3820. IF-0.84, (In press).

Manuscript under Communication

1. Siddiqui Sahabjada*, Mahdi AA, Arshad M. "Soy isoflavone genistein contributes to

enhanced osteogenesis via activating cell cycle progression, regulating oxidative stress and
attenuates osteoclastogenesis". CTIN-D-18-00335; Calcified Tissue International, 2018
(Under Review).
2. Jigyasu AK, Siddiqui Sahabjada, Jafri A, Arshad M, Lohani M, Khan IA. “Biologically
synthesized CdTe quantum dots induce G2/M cell cycle arrest and inhibit the growth of
human prostate carcinoma cells” BTER-D-18-00766; Biological Trace Element Research,
2018 (Under Review).
3. Harshita, Barkat MA, Rizwanullah M, Beg S, Siddiqui Sahabjada, Ahmad FJ. Dietary
Lipid-Based Nano Carriers of Paclitaxel with Improved Anticancer activity against Human
Liver Carcinoma. NNM-2018-0191. Nanomedicine, 2018 (Under Review).
4. Rahman MA, Sahabjada, Arshad M, Akhtar J. Inhibition of proliferation of human prostate
carcinoma cell, PC3 by Bauhinia racemosa Lam via induction of apoptosis. LDCT-2018-
0419. Drug and Chemical Toxicology, 2018 (Under Review).

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Conference abstracts/presentation
I published approx. 40 abstracts in both international and national conferences.

Member of organizing committee

1. 4th Seminar cum Training Workshop on “Recent Techniques in Molecular & Cell
Biology” November 23-26, 2016, Department of Zoology, University of Lucknow,
Lucknow, sponsored by DST-PURSE, New Delhi and Indian Society of Cell Biology, BHU,
Varanasi, India.
2. 3rd Hands-on training workshop on “Recent Techniques in Molecular & Cell Biology”
November 11-13, 2014, Department of Zoology, University of Lucknow, Sponsored by
Indian Society of Cell Biology, BHU and Centre of excellence, New Delhi, India.
3. 2nd Hands-on training workshop on “Recent Techniques in Molecular & Cell Biology”
March 11-13, 2013, Department of Zoology, University of Lucknow, Sponsored by Indian
Society of Cell Biology, BHU, Varanasi, India.
4. 1st Seminar cum Training Workshop on “Recent Techniques in Molecular & Cell Biology”
February 27-29, 2012, Department of Zoology, University of Lucknow, Lucknow, sponsored
by DST-PURSE, New Delhi and Indian Society of Cell Biology, BHU, Varanasi, India.

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References

Dr. Md Arshad Dr. Monisha Banerjee

(PhD Supervisor) (Professor)
Molecular Endocrinology Laboratory Molecular Human Genetics Lab
Department of Zoology Department of Zoology
University of Lucknow University of Lucknow
Lucknow-226007, India Lucknow-226007
E-mail- [email protected] Email- [email protected]

Dr. Aamir Nazir

(Senior Scientist)
Laboratory of Functional Genomics and Molecular
Toxicology, Division of Toxicology, CSIR-Central
Drug Research Institute, Lucknow-226001, India
E-mail- [email protected]

Declaration: I hereby declare that the particulars furnished herein by me are true to the best of
my knowledge and belief.

Place: (Sahabjada)
Date:

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