Centre Number Candidate Number Name

UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS

General Certificate of Education Advanced Level
BIOLOGY 9700/05
Paper 5 Practical Test A2
May/June 2006

1 hour 30 minutes
Candidates answer on the Question Paper.
Additional Materials: As listed in the Instructions to Supervisors.

READ THESE INSTRUCTIONS FIRST

Write your Centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use a pencil for any diagrams, graphs or rough working.
Do not use staples, paper clips, highlighters, glue or correction fluid.

Answer both questions.

You are advised to spend 45 minutes on each question.

At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.

For Examiner’s Use

TOTAL

This document consists of 8 printed pages.

SP (SC/CGW) T10866/2
© UCLES 2006 [Turn over
 2 For
Examiner’s
Use
1 You are required to investigate some aspects of respiration in germinating seeds using the
apparatus shown in Fig. 1.1.

0 1 2 3 4 5 6 7 8 9 10
soda lime
plastic or rubber tubing

germinating seeds gauze capillary tube drop of coloured

liquid

Fig. 1.1

You are provided with some germinating seeds and a syringe in which some soda lime has
been placed and a length of glass capillary tube attached.

Warning: do not remove the soda lime from the syringe as it will burn your skin.

Remove the plunger and insert the germinating seeds into the syringe. Replace the plunger
so that it touches the seeds.

Dip the end of the glass capillary tube into the coloured liquid, labelled L1, and very gently
pull on the syringe plunger so that a drop of liquid enters the capillary tube. Remove any
excess liquid with a paper towel. The soda lime will not be affected by small amounts of
coloured liquid.

Place the apparatus on a sheet of white paper alongside a mm ruler.

Your apparatus should now look like that shown in Fig. 1.1.

Wait until the drop of coloured liquid starts to move.

If the drop does not move within one minute, add more germinating seeds to the syringe and
set up the apparatus again.

© UCLES 2006 9700/05/M/J/06

 3 For
Examiner’s
Use
(a) Mark the position of the coloured liquid on the capillary tube.

Measure how far the liquid moves in one minute.

Repeat the measurement every minute for the next four minutes.

If the drop reaches the end of the capillary tube, remove the plunger from the syringe
and reset the apparatus with a fresh drop of coloured liquid.

Construct a table in the space below and record your results, showing the mean distance
moved per minute by the drop of coloured liquid.

[4]

(b) A student carried out the same experiment using the apparatus shown in Fig. 1.2.

syringe

screw-clip
scale valve

compensation
germinating tube
seeds

glass
beads
soda lime

manometer

Fig. 1.2

© UCLES 2006 9700/05/M/J/06 [Turn over

 4 For
Examiner’s
Use
The following data was obtained with the apparatus as shown in Fig. 1.2.

Droplet moved 1.8 mm per min–1 towards the syringe.

The soda line was removed from the test tube containing the seeds and replaced with
an equal volume of glass beads.

Droplet moved 1.0 mm in 5 minutes away from syringe.

Each 10 mm of capillary tube has a volume of 8 mm3.

(i) Calculate the volume of oxygen produced.

Show your working.

..............................................................................................................................[1]

(ii) Calculate the volume of carbon dioxide consumed.

Show your working.

..............................................................................................................................[3]

(iii) Calculate the RQ of the germinating seed from the data provided.
Show your working.

..............................................................................................................................[1]

(c) Explain the advantage of using the apparatus shown in Fig. 1.2, rather than the simpler
respirometer that you used in Fig. 1.1.

..........................................................................................................................................

..........................................................................................................................................

..........................................................................................................................................

......................................................................................................................................[1]

© UCLES 2006 9700/05/M/J/06

 5 For
Examiner’s
Use
(d) A student carried out a similar experiment using green pond weed. The soda lime and
glass beads were replaced with a series of different concentrations of sodium hydrogen
carbonate solution. The apparatus is shown in Fig. 1.3.

syringe

bright
light scale screw-clip
valve

compensation
pond weed tube

sodium sodium
hydrogen hydrogen
carbonate carbonate
solution solution
manometer

Fig. 1.3

(i) Fig. 1.4 is a graph showing the rate of movement of the manometer fluid, against
sodium hydrogen carbonate concentration.

rate of movement of
manometer fluid

sodium hydrogen carbonate concentration

Fig. 1.4

Fully explain the shape of the graph.

..................................................................................................................................

..................................................................................................................................

..................................................................................................................................

..................................................................................................................................

..............................................................................................................................[3]

© UCLES 2006 9700/05/M/J/06 [Turn over

 6 For
Examiner’s
Use
(ii) The student knows that the equation for photosynthesis is:

6H2O + 6CO2 C6H12O6 + 6O2

The student thought that as the volume of carbon dioxide used equalled the volume
of oxygen produced, the manometer fluid should remain still.
Suggest why the manometer fluid did not remain still.

..................................................................................................................................

..................................................................................................................................

..............................................................................................................................[2]

[Total: 15]

© UCLES 2006 9700/05/M/J/06

 7 For
Examiner’s
Use
2 S1 is a slide of a transverse section of a leaf.

(a) Make a large, plan diagram to show the distribution of the tissues using the eyepiece
graticule to help you represent the proportions of the structures.

[6]

(b) Using the ruler to measure both specimen and drawing, calculate the magnification of
your drawing. Indicate clearly on your drawing where you took the measurements.
Show your working.

magnification ………………………………………[2]

© UCLES 2006 9700/05/M/J/06 [Turn over

 8 For
Examiner’s
Use
(c) Use the high-power of your microscope and make a large, annotated drawing of
one of the stomata and surrounding guard cells, indicating those features which are
characteristic of xerophytes.

[5]

(d) Describe two other features visible in the transverse section of the leaf which are
characteristic of xerophytes.

1 .......................................................................................................................................

..........................................................................................................................................

..........................................................................................................................................

2 .......................................................................................................................................

..........................................................................................................................................

......................................................................................................................................[2]

[Total : 15]

© UCLES 2006 9700/05/M/J/06