Centre Number Candidate Number Name

UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS

General Certificate of Education
Advanced Subsidiary Level and Advanced Level
BIOLOGY 9700/03
Paper 3 Practical Test AS
May/June 2005

1 hour 15 minutes
Candidates answer on the Question Paper.
Additional Materials: As listed in Instructions to Supervisors.

READ THESE INSTRUCTIONS FIRST

Write your Centre number, candidate number and name in the spaces provided at the top of this page.
Write in dark blue or black pen in the spaces provided on the Question Paper.
You may use a soft pencil for any diagrams, graphs or rough working.
Do not use staples, paper clips, highlighters, glue or correction fluid.

Answer both questions.

The number of marks is given in brackets [ ] at the end of each question or part question.
You are advised to spend 40 minutes on Question 1 and 35 minutes on Question 2.

For Examiners Use

Total

This document consists of 7 printed pages and 1 blank page.

SP (CW/KS) S84445/4
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1 You are required to investigate the effect of a bacterial digestive enzyme on protein.

You are provided with one test-tube containing a solution labelled S1 and three test-tubes
containing a solution labelled S2. Both solutions, S1 and S2, contain the bacterial enzyme at
pH7, but solution S1 has been boiled.

You are also provided with four lengths of glass capillary tubing that contain solidified egg
white. Egg white contains the protein albumin.

Place one length of capillary tubing into each test-tube as shown in Fig. 1.1. Avoid getting
any solution on your skin.

Fig. 1.1

Place the test-tube labelled S1 into a water bath at approximately 35 C.

Place one of the test-tubes labelled S2 into a water bath at approximately 45 C.

Place two of the test-tubes labelled S2 into a water bath at approximately 35 C.

To one of the 35 C test-tubes labelled S2 add dilute acid drop by drop until it has a pH of
less than 3. Count the drops. Use universal indicator paper to check the pH. Label this test-
tube pH3.

Add the same number of drops of distilled water to each of the other three test-tubes.

(a) (i) Explain why is was necessary to add the same number of drops of distilled water to
the other three test-tubes.

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...............................................................................................................................[1]

Leave the solutions for at least thirty minutes.

While you are waiting, you may start question 1 (b).

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(ii) Gently shake each test-tube. Carefully examine the capillary tubes, still within the
test-tubes, in front of the dark coloured card. Draw what you see in the boxes
below.

capillary tube in S1 at 35 C at pH7 capillary tube in S2 at 35 C at pH7

capillary tube in S2 at 45 C at pH7 capillary tube in S2 at 35 C at <pH3

[2]

(iii) Give a detailed explanation of your results.

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...............................................................................................................................[3]

UCLES 2005 9700/03/M/J/05 [Turn over

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(b) A student carried out a similar experiment for a longer period of time, using a range of
buffer solutions. The results are shown in Table 1.1.
Table 1.1

pH length of egg white remaining / mm length of clear tube / mm

6 20 10

7 17 13

8 15 15

9 23 7

10 26 4

11 28 2

(i) Plot a graph of the pH against one of the other variables on the grid below.

[3]

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(ii) Explain why the rate of reaction changes with pH.

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...................................................................................................................................

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...............................................................................................................................[3]

(c) Explain how the experiment could be modified to determine the effect of enzyme
concentration on the rate of the reaction.

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......................................................................................................................................[3]

[Total : 15]

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2 K1 is a stained transverse section through part of a dicotyledonous plant.
Examine the specimen using the low-power of your microscope.

(a) (i) Draw a large, labelled, plan diagram to show the distribution of tissues.

[5]

(ii) State from which part of the plant the section was taken.
Explain your answer.

...................................................................................................................................

...................................................................................................................................

...............................................................................................................................[2]

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(iii) Make a high-power drawing to show a group of four cells from the region nearest
the centre of the specimen.
Labels are not required.

[3]

[Total : 10]

[Paper total : 25]

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BLANK PAGE

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University of Cambridge International Examinations is part of the University of Cambridge Local Examinations Syndicate (UCLES), which is itself a department of
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9700/03/M/J/05