LOCAL DEVELOPMENT OF A HERMETIC STORAGE

CONTAINER FOR QUALITY PRESERVATION

OF RICE SEEDS IN THE PHILIPPINES
Ricardo F. Orge and John Eric O. Abon
Philippine Rice Research Institute
Maligaya, Science City of Muoz, Nueva Ecija, Philippines.
Corresponding author: [email protected]
Available at http://www.ssrn.com/link/OIDA-Intl-Journal-Sustainable-Dev.html
ISSN 1923-6654 (print) ISSN 1923-6662 (online).
Ontario International Development Agency, Canada. Author et al

Abstract: Experiences in the implementation of the Philippine governments Hybrid Rice

Commercialization Program (HRCP) showed that storing rice seeds under a hermetic (gas tight)
condition using an imported flexible plastic-lined container can effectively prevent storage pest
infestation as well as protect the seeds from the quality-deteriorating effect of high humidity
during rainy season. However, the available technology is expensive thus limiting its adoption by
the local rice seed producers (RSPs). Hence, this study was conducted to develop a low cost
alternative hermetic seed storage technology which can easily be mass fabricated locally. A
prototype of a hermetic seed storage container (HSSC), equipped with a simple zipping
mechanism, was developed and tested to evaluate its performance in comparison with the other
seed storage practices/technologies. Results of the three one-year storage trials showed that the
HSSC performed consistently and significantly better than the conventional practice (open-piled
bags under ambient condition) and comparably with the imported hermetic container (IHC) in
maintaining the viability and vigor of the stored rice seeds (hybrid and inbred varieties) as well as
in preventing storage pest infestation.
Keywords: hermetic storage; hermetic container; hybrid rice seeds; seed quality; seed storage
INTRODUCTION

he Philippines needs an increased supply of rice primarily because of its growing population. This is a
challenge that needs to be seriously taken especially that its rice lands are decreasing due to urbanization and
industrialization [1]. Realizing this fact, the government introduced the hybrid rice technology as a new
approach for increasing rice production, farmers productivity and competitiveness, mitigating the negative effects
of the El Nino phenomenon over the short term, and attaining national food security over the long term [2]. Hybrid
rice is known to have a yield advantage of 15% against the inbred varieties, under the same input levels [3].

In 2002, with the implementation of the Hybrid Rice Commercialization Program (HRCP), the
commercialization of the hybrid rice technology became the Philippine agricultures banner program in attaining
self-sufficiency and increasing productivity and profitability in rice, and generating rural employment [4]. Since the
implementation of the HRCP, more and more farmers are convinced that hybrid rice can indeed increase their yields
and incomes. In Northern Luzon, survey showed that farmers get an average net profit which is Php16,069 (~US$
380) ha-1 higher than what they get from inbred rice [5].
Ensuring the availability of good quality hybrid rice seeds at the time farmers need them for planting was
one of the critical problems encountered during the implementation of the HRCP. Because of some protocols that
had to be followed to ensure the production and distribution of good quality seeds (time isolation in planting, seed
certification process, etc.), the availability of the hybrid rice seeds was often delayed and did not match with the
time farmers needed them for planting. Because of this, majority of the produced seeds had to be stored for the next
planting season. Maintenance of seed quality during storage had been a persistent problem because of inadequate
storage facilities. Quality deterioration due to storage pest infestation and exposure of the seeds to high relative
humidity during the rainy seasons were the most common problems.
To help solve this problem, the government, through the Philippine Rice Research Institute (PhilRice)
being the procurer and the distributor of the hybrid rice seeds during the initial years of the HRCP implementation,
adopted, among other things, the use of hermetic storage technology in storing the excess seeds to augment its

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limited cold storage facilities. This technology makes use of an imported plastic-lined container where the 20-kg
bags of hybrid rice seeds were stored inside. It was first adopted in the Philippines by the National Food Authority
after being evaluated to effectively preserve the quality of stored milled rice without employing fumigants [6].
Studies also had been conducted for outdoor hermetic storage of corn and paddy and showed that the quality and
viability of the seeds was preserved up to six months [7]. Follow-up studies [8] and experiences from actual use of
this technology by the authors in storing hybrid rice seeds under the HRCP further confirmed these findings.
Hermetic storage is an environment-friendly technology, involving no hazard to the storage workers,
consumers, and non-target organisms. With it, the use of insecticides particularly fumigants, which are being
questioned because of their effect on humans and the environment [9] are eliminated. Using this technology,
because the container is sealed and impermeable to gases, the stored seeds are freed from the quality-deteriorating
effects of humid environments. Moreover, the respiration of the seeds and of the infesting insects lowers down the
oxygen concentration to a level where it becomes insecticidal. For the majority of stored product insects and their
developmental stages, this oxygen level is within the range of 2% to 4% [10]. The low oxygen environment could
control insect infestations but preserve the quality of the grains [11]. On the other hand, high oxygen level tends to
hasten viability loss, especially in seeds with high MC [12].
Since the governments intervention on the HRCP was only short term, there was a need to enhance the
capability of the hybrid RSPs to store their excess seeds. The establishment of cold storage facilities requires high
investments and operating costs such that it is not practical to be used at the level of most local RSPs. In contrast,
hermetic storage requires little or no maintenance cost, however, the current technology available in the market is
not affordable to the local RSPs as very few has so far invested on it in spite of the known benefits it offers.
While a lot of literatures related to hermetic storage had already been published [13, 14, 15, 16], only few
deal on the design and development aspect of the technology, particularly on the kind of material used in a storage
container to effect a hermetic condition. One example of these hermetic storage technologies is the one developed
by Villers [17] for long term storage of a bulk commodity which utilized a flexible, low permeability sheet material.
Further enhancements and variants of this hermetic storage technology was done in succeeding years [18, 19, 20,
21]. On the other hand, Navarro et al. [22] incorporated a compound in the manufacture of a packaging material
suitable for use in hermetic storage enclosures. While these technologies have been proven to be effective, these are
developed in advanced countries and their adoption in developing countries like the Philippines may be limited due
to the low purchasing capacity of the local RSPs who are the target users of the technology. Adhikarinayake et al.
[23], on the other hand, attempted to develop an air tight storage bin made of ferro-cement which may be
economically feasible for farmers in developing countries. The bin worked well for storing commercial paddy,
however, further studies need to be done since the germination rate of stored paddy dropped from 85% to 0% after 6
months of storage.
This paper generally aimed to design and develop a low cost alternative for hermetic storage technology.
Specifically, it aimed to:
1. Come up with a design of a flexible HSSC that can be fabricated using locally available materials and
fabrication equipment and can perform comparably with the imported hermetic container (IHC).
2. Test the performance of the final prototype of the HSSC together with the existing seed storage methods.
MATERIALS AND METHODS
Prototype design and fabrication. To be able to come up with a low cost technology on hermetic storage, the
development of the local HSSC was based on the following design criteria:
1. Allows local fabrication. There were two concerns seriously considered so that the hermetic requirement
and ease of mass fabrication could be satisfied: (a) the use of a locally available, inexpensive but durable
material, and (b) a simple but durable zipping mechanism;
2. Easy to operate and maintain. The HSSC was designed to be easy to set up as well as repair and maintain by
the target users (RSPs) themselves.
Performance evaluation of storage technologies/practices. The technical feasibility of the HSSC was evaluated
together with the existing seed storage practices/technologies (Table 1). In all of the storage trials, the rice seeds
were contained in the commonly used 20-kg closely woven polypropylene plastic sacks with 0.1mm thick internal
polyethylene plastic liner.
1. Conventional storage (CS). In this method, six bags of rice seeds were piled on top of a 0.15m x 1.2m x
1.2m wooden pallet and stored openly under ambient conditions inside a warehouse.
2. Imported hermetic container (IHC). Rice seeds were piled and stored inside a 2.95m x 1.70m x 1.5m
(length, width, and height, respectively) flexible container made of 0.83mm Polyvinyl Chloride (PVC)

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13

plastic liner equipped with a multiple tongue and groove zipping mechanism. The pile consisted of eleven
layers of bags with 21 bags in each layer.
3. Cold room storage (CRS). The bags of rice seeds were piled on top of a wooden pallet and stored inside a
6m wide x 9m long x3m height cold storage room with digital temperature and RH recorder set at 15oC and
50%, respectively.
Table 1. Storage trials conducted to evaluate the performance of the prototype HSSC
together with other existing seed storage practices/technologies.
Trial No.
1
2
3

Storage Practices Evaluated

HSSC and IHC
CS, HSSC and IHC
CS, HSSC and IHC

Rice Varieties Used

PSB Rc72H
PSB Rc72H
NSIC Rc116H & PSB Rc82

Conduct of storage trials. All of the storage practices/technologies evaluated were set up inside a warehouse of the
Philippine Rice Research Institute, Munoz Science City, Nueva Ecija, Philippines (approximately 15.71oN latitude
and 120.90oE longitude). A total of three one-year storage trials were conducted, one after the other, incorporating
prototype design improvements in every succeeding trial based on problems and weaknesses observed in the
preceding trial. Seeds of a local hybrid rice variety, PSB Rc72H, were used in the first and second storage trials,
being the most abundant hybrid rice seeds available at that time. One kg samples for seed quality analysis were
taken from the randomly selected bags representing the top, middle, and bottom portion of the pile. Sampling was
done at 0, 3, 6, 9, and 12 months of storage. These samples were analyzed in the PhilRice seed laboratory for
various performance parameters discussed below.
Performance parameters. The performance of the prototype HSSC was evaluated and compared with the other
storage technologies/practices in terms of the following parameters:
1. Hermetic capability. In the absence of appropriate facility for conducting laboratory tests to evaluate the
gas tightness of a container, the capability of the prototype HSSC to maintain a hermetic condition was
determined by simply monitoring two parameters, namely, the oxygen concentration of the air and the MC
of the seeds inside the container. This is based on the theory that, for a hermetic container, there would be
no (if not very minimal) exchange of gases between the inside and outside environments thus leading to the
reduction in the oxygen level of the air inside the container due to seed and insect (if present) respiration.
Likewise, there would also be no significant change in the seed MC since the stocks are isolated from the
influence of the humidity of the ambient air. Hence, the hermetic property of the container would have
direct influence on the oxygen concentration of the air as well as the MC of the seeds inside the container.
In each of the storage trial, monitoring of the oxygen level of the HSSC and of the IHC (as basis of
comparison) was done at least three times a week, throughout the one-year storage duration, using a
GrainPro oxygen meter. Wireless and programmable data logger (ERTCO MicroRHTemp) was installed
inside the warehouse to measure the ambient air temperature and RH. On the other hand, seed MC was
gathered after every three months where sampling is regularly done to monitor the status of the quality of
the seeds. Seed MC was measured immediately after collecting the samples using a G-Won grain
moisture meter earlier calibrated through oven drying method. Three readings were taken per sample and
the average was calculated and expressed in percentage of the weight of the original sample (wet basis).
2. Seed germination and vigor. The germination percentage of seeds was determined using the rolled paper
method while the seed vigor using the accelerated aging test described in the International Seed Testing
Association Manual [24].
3. Degree of insect infestation. This was evaluated by counting the number of insects, regardless of species,
present in a collected sample. For each storage practice, one kg samples were drawn from designated
sampling bags that were randomly situated at the top, middle, and bottom layers in the piled bags of seeds.
Five hundred-gram samples were gathered from each sampling bag and then put in the freezer so as to kill
the live insects and facilitate their easy separation by sieving. Sifted insects were then counted and
expressed in terms of their number per kg of seeds.

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RESULTS AND DISCUSSION

The test prototypes. The identified locally available material for the prototype HSSC was 0.8mm thick PVC
tarpaulin sheet. Based on manufacturers specifications, this material has the following characteristics: waterproof,
high intensity and flexibility, good tensile/tear and peeling strength, with ultra-violet protection, and with resistance
to high temperature. No data on gas permeability was available. For PVC materials, however, the O2 permeability
coefficient is 5 cm3 mm/m2day Atm 20-25oC [25] which is the lowest among plastic lined materials available in the
local market. This material can easily be found in most local stores and sold in per length (width=1.8m) or per roll
(total length=50m) basis. Fabrication of the prototypes was done by a supplier of PVC tarpaulin sheets which was
also fabricating tarpaulin-based products like outdoor tents, car covers, boat covers, and many others. Fabrication
consisted of cutting the sheet into the desired shapes and then welding the edges using an electrically heated
tarpaulin sealer.
Table 2 briefly describes the yearly progress made in the development of the HSSC, starting from the first
prototype (trial 1) up to the final one (trial 3). For the first and second storage trials, the test prototypes were
composed of identical top and bottom components with a simple zipping mechanism that made use of a corrugated
flexible plastic tube with longitudinal slit, enabling it to clamp and hold the two joined edges of the two components
around one cm distance from the folded section. In trial 1, modeling clay was added at the clamped edges to seal
any possible leak, however, this was removed in the second trial since it was found out from a separate laboratory
experiments that even without sealing the edges with clay, the clamping tube could already establish a sustained low
oxygen condition inside the container. For the third trial, further design improvement was done after noting that the
corrugated plastic tube used for the zipping mechanism did not last long. Thus, other alternative materials were
being considered which finally lead to the use of Velcro strips. The Velcro strip is popularly used in the garment
industry as a good substitute for buttons or zippers. The use of the Velcro strip for the prototypes zipping
mechanism demanded a change in the design, from two components (top and bottom) to only one so as to facilitate
easy opening and closing. Although a different zipping material was used, the sealing principle adopted in the
previous prototypes (i.e. folding and clamping the joined edges together) remained the same. The resulting
prototype, which was considered as the final prototype, was also reduced in size to accommodate 50 bags (20
kg/bag) which is the size of 1 seed lot, basing from the Philippine seed certification standards. With this size, the
filled container can already be put on top of a standard 1.2m x 1.2m pallet to facilitate movement from one place to
another. Figure 1 shows how the final prototype is used.
Table 2. Descriptions of the HSSC prototypes used in each storage trial.
Prototype/
Trial No.

Dimension, m
(LxWxH)

No. of
components

1.75 x 1.75 x 1.75m*

Clamping the edges with the

longitudinal slit of a flexible
pipe then sealing the joined
edges with modeling clay

1.75 x 1.75 x 1.2m*

Clamping the edges with the

longitudinal slit of a flexible
pipe, no sealing with
modeling clay

3
1.35 x 1.35 x 2.05m
*Dimension of each component

Velcro strip

Sealing mechanism

Capacity,
(number of 20-kg
bags)
200

144

48-50

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15

Figure 1. Diagram showing how the HSSC (final prototype) is used.

Prototype performance. In general, basing from the three storage trials conducted, the HSSC prototypes tested
were able to maintain a low O2 concentration as well as low seed MC and insect count which were comparable to
that of the IHC and better that the CS. The following discussions however focus more on the results of the third
storage trial wherein the performance of the third and final prototype of the HSSC was evaluated and compared with
the other storage practices/technologies.
Hermetic capability. Figure 2 shows a profile of the oxygen level inside the two hermetic containers. As shown,
the oxygen level of the air inside HSSC is comparable to that of the IHC. The graph shows some peaks (at normal
oxygen level of 21%) which were the time the containers were opened for sampling after every 3 months.

Figure 2. Comparison between the oxygen level inside the prototype HSSC and its imported counterpart.
In all of the three trials conducted, the MC of the rice seeds kept inside the two hermetic containers (IHC
and HSSC) did not significantly change after a year of storage, as analyzed statistically. However, for those stored in
the CS, particularly referring to the data gathered in the third trial, there was already a significant increase in the
seed MC of PSB Rc82 after 6 months of storage (Figure 3). Finally, after 12 months, the seed MC rose 21.6%, an
average increase of 1.8% per month starting from an initial MC of 12.73%. The rise in MC for the seeds stored

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Orge and Abon OIDA International Journal of Sustainable Development 07:09 (2014)

under CS can be attributed to two factors, namely, (a) moisture absorption by the seeds since they were exposed to
humidity of as high as 100% during the rainy days, and (b) insect pest infestation.

Figure 3. Moisture content profile of PSB Rc72H hybrid rice seed samples taken from
the three storage methods every after 3 months.

Seed viability. Overall results of the three storage trials conducted showed that the locally developed HSSC could
preserve the viability of stored seeds significantly better than the CS and comparably with the IHC and the CRS.
For the third trial in particular, as shown in Table 3, there was already a statistically significant decline in the
germination percentage of the conventionally stored seeds after 6 months of storage for the hybrid variety (NSIC
Rc116) and 9 months for the inbred variety (PSB Rc 82). This finding conforms to that of Sabio et al. [26] and
Chang [12].
Table 3. Seed germination rate of the two rice varieties stored under different storage methods*.
a. NSIC Rc116H (hybrid)
STORAGE
METHOD
0
HSSC
86.13a
IHC
86.02a
CS
85.93a
CRS
85.57a

3
91.10a
89.53a
91.43a
92.23a

Months of storage
6
93.67a
89.90a
57.77b
93.10a

9
94.90a
88.53a
19.23b
92.90a

12
94.67a
84.02a
3.43b
98.00a

b. PSB Rc82 (inbred)

Months of storage
STORAGE
METHOD
0
3
6
9
12
HSSC
96.59a
95.00a
95.53a
95.66a
95.92a
IHC
96.75a
94.82a
92.90a
90.26a
85.56a
CS
96.58a
94.32a
86.02a
76.05b
45.03b
CRS
96.70a
95.65a
95.89a
97.13a
97.78a
*Values in a column with the same letter are not statistically different according to Duncans Multiple
Range Test at p < 0.01.

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17

Seed vigor. Vigor is recognized as an important seed quality factor distinct from germination [27]. Results of
accelerated aging tests to evaluate seedling vigor (Table 4) also showed the same trend as in the percentage
germination.

Table 4. Germination of two rice seed varieties stored under different storage methods
after being subjected to accelerated ageing tests
a. NSIC Rc116H
STORAGE
METHOD
HSSC
IHC
CS
CRS

0
76.33a
75.44a
78.78a
71.44a

MONTHS OF STORAGE
3
6
9
91.00a
93.44a
94.56a
86.89a
88.78a
86.56a
89.22a
60.22b
23.44b
88.22a
91.78a
93.11a

12
87.78a
86.33a
7.33b
93.89a

b. PSB Rc82
STORAGE
METHOD
HSSC
IHC
CS
CRS

0
94.81a
94.70a
93.82a
94.84a

MONTHS OF STORAGE
3
6
9
93.09a
94.91a
96.18a
92.59a
92.83a
90.57a
93.09a
79.98b
77.07b
94.21a
96.06a
96.69a

12
93.07a
89.74a
38.98b
94.53a

Insect count. A combination of rice weevils (Sitophilus oryzae L.) and lesser grain borers (Rhizopertha dominica
F.) was observed in all of the taken seed samples. Among the storage practices/technologies evaluated, CRS did
not exhibit a significant increase in insect count for the one year storage period (Table 5). A significant increase in
seed count was observed in the hermetically stored seeds starting at the third month of storage but it did not
progressed much as compared to the conventionally stored seeds.
Table 5. Average insect count per 500 g sample of seeds from two rice varieties stored
under different storage methods.
a. NSIC Rc 16H
STORAGE
METHOD
Prototype
Imported
Conventional
Cold storage

0
0.33a
0.33a
0.20a
0.33a

MONTHS OF STORAGE
3
6
9
3.33a
10.67a
13.33b
7.33a
17.33a
75.00c
319.00b
523.67b
749.33d
1.00a
2.00a
1.67a

12
22.67b
63.67c
899.67d
5.33a

0
0.67a
0.40a
0.67a
0.67a

MONTHS OF STORAGE
3
6
9
4.00b
3.00b
24.33b
12.00b
10.00b
38.67b
225.33c
266.00c
311.67c
1.33a
0.67a
1.33a

12
34.00b
41.00b
302.67c
3.00a

b. PSB Rc 82
STORAGE
METHOD
Prototype
Imported
Conventional
Cold storage

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Orge and Abon OIDA International Journal of Sustainable Development 07:09 (2014)

SUMMARY, CONCLUSION, AND RECOMMENDATIONS

This study was conducted to locally develop a hermetic seed storage technology for preserving the quality
of rice seeds during long term storage.
Results of storage trials conducted to evaluate the performance of the locally developed HSSC in
comparison with the imported counterpart (IHC) as well as the other storage technologies/practices (CS and CRS)
showed that the HSSC could preserve the quality of stored inbred and hybrid rice seeds comparable with that of the
IHC and significantly better than the CS method. The study only focused on evaluating the performance of the
HSSC based on the practical aspects particularly on how they perform at the target users perspectives. Follow up
laboratory trials need to be done to have a deeper understanding of its capability and performance relative to the
imported counterpart particularly in terms withstanding the leak tests, determining both the O2 and CO2
concentration of the air inside it during actual usage.
ACKNOWLEDGEMENT
This research was funded by the Philippine Department of Agriculture.
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