Integrating Seaweeds Into South African Abalone Aquaculture
Integrating Seaweeds Into South African Abalone Aquaculture
Integrating Seaweeds Into South African Abalone Aquaculture
John J. Bolton1, Deborah M. Robertson-Andersson1, Max Troell2, Robert J. Anderson3, Gavin Maneveldt4, Christina Halling2, AJ Smit5, Trevor Probyn3 & Sue Peall1
1University 2Stockholm 3Marine
of Cape Town, South Africa University, Sweden of the Western Cape, South Africa of KwaZulu-Natal, South Africa
4University 5University
Overview
Abalone farming in South Africa Importance of seaweeds (kelp) as feed in SA abalone farming Prospects for aquacultured seaweeds as feed
Tons
3000 2500 2000 1500 1000 500 0 1948 1951 1954 1957 1960 1963 1966 1969 1972 1975 1978 1981 1984 1987 1990 1993 1996 1999 2002 2005
Abalone farming in South Africa has developed rapidly and the country is now the second largest producer outside Asia.
World Aquaculture March 2003
Durban
West Coast Abalone Seige Investments Blue Cap General Trading Jacobsbaai Sea Products
Cape Town
Foster Keith John Avuca Abalone Fish Bay Mariculture HIKAbahowe Farm Aquafarm Development Hermanus Abalone Dezzo Trading
Port Elizabeth
Photos: RJ Anderson
600 T o n s a b a lo n e 500 400 300 200 100 0 1986 1987 1988 1989 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003
Abalone production
T o n s k elp
Concession MSY Harvest Total Area (t f wt) (t f wt) Harvest as % of MSY 5 (3) 1165 696 60 6 (3) 2680 897 33 7 (3) 644 348 54 8 (3) 956 951 99 11 (2) 1550 1158 75 Totals 10399 4050 52
ARTIFICIAL FEED: ca. 6% - mostly ABFEED: - (Marifeed Property Ltd., South Africa) - Formulated feed containing fishmeal, soya bean meal, starch, vitamins and minerals
Initial research on south coast, where there is no kelp (University of Port Elizabeth, now NMMU), more recently in current project Our research has shown that feeding cultivated seaweeds to abalone had a number of benefits: Increased growth rates, Improved vigour and stress resistance
Seaweed cultivation
Abalone weight (g) on different diets after 9 months ( Naidoo et al. J. Appl . Phycol ., in press) (Naidoo Appl. Phycol.,
30 28 26 24
22 20 18 16 14 12 10 8 6
-0 2
-0 2
-0 2
03
A ug -0
ar -0 3
3 A pr -0
Fe b-
N ov
D ec
Se p
Ja
ay -0
ct -0
n0
Growing abalone feed in South Africa? A reality: Wild Coast Abalone (south coast, near East London)
Trace concentrations of a volatile sulphur compound, Dimethylsulfide (DMS), were discovered in the Earth's atmosphere about three decades ago. The gas was also found to occur in ocean surface waters. Some phytoplankton synthesize the molecule dimethylsulfoniopropionate (DMSP) DMS is released by algal cells following death and sloppy feeding by zooplankton. DMS a naturally produced biogenic gas essential for the Earth's biogeochemical cycles.
H C C
OH DMSP
DMS can be formed by the enzymatic cleavage of DMSP to DMS and acrylate, as well as by the oxidation of DMSP with OH-, oxygen or hydrogen peroxide
DMSP is not produced by animals DMSP may accumulate in some herbivores following feeding on a DMSP-rich diet DMSP is taste- and odourless Offensive, off tastes and smells are associated with DMS Animals lack the enzyme DMSP-lyase that converts DMSP to DMS DMS may be produced from DMSP post mortem in animals via: bacterial DMSP-lyase activity (decay) heat treatment (e.g. canning)
Preliminary investigations
Giant clams (which have zooxanthellae) developed off-odours after death due to DMSP
Preliminary investigations
Is DMS was detectable in abalone? 1 2 g of tissue was removed from 5 abalone fed a mixed (kelp, Ulva and Gracilaria) diet. Measurement of DMS: Quantitative conversion of DMSP to DMS through alkali hydrolysis DMS analysis via gas chromatography
p To
ot o f f o
Preliminary investigations
DMSP values from various tissue types in mixed diet cultivated abalone
35 30
AM BF F
DMSP ( g.g-1)
25 20 15 10 5 0 BF AM
TF
TF
Diet experiment
Diet DMSP
(mmol.kg
-1)
Abalone DMSP
(mmol.kg
-1
Kelp Kelp + Ulva +Gracilaria Abfeed Ulva Kelp + Abfeed Gracilaria + Ulva Gracilara + kelp
0.2 + 0.1
20 5 10 10 10 5 5
0 35.3 + 10.6
0.1 (grac)
Diet experiment
Values for tridacnid clams are 30 mmol.kg -1 (can be 5060 mmolkg1), (previously the highest recorded in animals: Hill et al. 2004)) Typical values for fish, crustaceans and other molluscs are below 3 4 mmol.kg-1 (Hill et al. 2000)
Diet DMSP
(mmol.kg
-1)
Abalone DMSP
(mmol.kg
-1
n 5 5 10
Kelp + Ulva +Gracilaria Gracilaria + Ulva Ulva 0.1 (grac) 35.3 + 10.6
Depuration experiment
250
200
150
100
50
Time (days)
Wild abalone
1500 1250
DMSP (g.g-1)
medium
small
large
Wild abalone and cultured abalone that eat seaweed have levels of DMSP Is DMS an important component in abalone for optimal taste? Taste tests ..
THANK YOU
ACKNOWLEDGEMENTS
Sweden/South Africa Collaborative Programme (SIDA/NRF) I & J Mariculture farm JSP Mariculture farm Abagold
JSP