Cryopreservation Technique for Conservation of Plant Germplasm: Its Possible Use in Indonesia. Semuel Leunufna. Increasing rate of plant germplasm lost in Indonesia has promoted the implementation of various methods for their...
moreCryopreservation Technique for Conservation of Plant Germplasm: Its Possible Use in Indonesia. Semuel Leunufna. Increasing rate of plant germplasm lost in Indonesia has promoted the implementation of various methods for their conservation. Cryopreservation is a technique applicable for a long-term preservation (base collection) of plants possessing non-orthodox (recalcitrant and semi-recalcitrant) seeds and those propagated vegetatively. The technique can be used as an alternative method for orthodox seed plants preservation in the ex situ conservation system. Although field and in vitro collection methods can be applied for the non-orthodox seed plants, a number of disadvantages possesed by these methods, especially in the tropics or the developing countries, deny their use for the establishment of a long-term germplasm collection. Successful implementation of the cryopreservation technique is supported by the development of protocols, which are able to provide a high recovery rate for species understudy, using vitrification based methods which are simple, economical, applicable to complex organs, and able to implement a high number of explants per experiment. The availability of infra-structures including in vitro culture laboratories, continue supply of liquid nitrogen is highly supporting the use of cryopreservation technique in Indonesia.
