Papers by Jorge Sahagun
Philippine Journal of Science
Phenotypic screening in plant breeding is essential for identifying superior mutant lines for orn... more Phenotypic screening in plant breeding is essential for identifying superior mutant lines for ornamental plant variety development. However, the process is tedious and time-consuming involving both qualitative and quantitative methods. To fast-track phenotypic screening, artificial intelligence (AI) presents a promising tool with higher efficiency and minimal standard error in identifying mutant lines for registration. Thus, in this study, an algorithm for classifying novel ornamental plant varieties is proposed. After training a convolutional neural network (CNN) model with the images of radiation mutant BG regale and control, Sansevieria rorida, comparisons of model loss and accuracy were done with Adam, SGD, RMSprop, and Adadelta optimization techniques. Among the optimization techniques, RMSProp achieved the lowest model loss (3.68%), the highest accuracy (98.44%), precision (98.44%), recall (98.39%), and F1-score (98.39 %). In this “proof-of-concept” study on the Philippines’ P...
Philippine Journal of Science
Phenotypic screening in plant breeding is essential for identifying superior mutant lines for orn... more Phenotypic screening in plant breeding is essential for identifying superior mutant lines for ornamental plant variety development. However, the process is tedious and time-consuming involving both qualitative and quantitative methods. To fast-track phenotypic screening, artificial intelligence (AI) presents a promising tool with higher efficiency and minimal standard error in identifying mutant lines for registration. Thus, in this study, an algorithm for classifying novel ornamental plant varieties is proposed. After training a convolutional neural network (CNN) model with the images of radiation mutant BG regale and control, Sansevieria rorida, comparisons of model loss and accuracy were done with Adam, SGD, RMSprop, and Adadelta optimization techniques. Among the optimization techniques, RMSProp achieved the lowest model loss (3.68%), the highest accuracy (98.44%), precision (98.44%), recall (98.39%), and F1-score (98.39 %). In this “proof-of-concept” study on the Philippines’ P...
Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Ph... more Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Philippines is described and illustrated. The foliage may appear similar to those of Hoya camphorifolia and Hoya bicolensis, but its flower does not resemble any known Hoya species to date.
Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Ph... more Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Philippines is described and illustrated. The foliage may appear similar to those of Hoya camphorifolia and Hoya bicolensis, but its flower does not resemble any known Hoya species to date.
Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Ph... more Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Philippines is described and illustrated. The foliage may appear similar to those of Hoya camphorifolia and Hoya bicolensis, but its flower does not resemble any known Hoya species to date.
Philippine Journal of Science, 2018
This study aimed to examine a promising procedure for in vitro direct organogenesis from floral t... more This study aimed to examine a promising procedure for in vitro direct organogenesis from floral tissues of Dendrobium Sonia 'Earsakul' as a new potential regeneration system for transformation in orchids. Petal and sepal explants from floral buds (2.0–2.3 cm) survived on ½ MS solid medium supplemented with or without 1-naphthaleneacetic acid (NAA) or/and benzylaminopurine (BA), but only petal explants generated protruding meristemoid tissues when they were cultured on ½ MS medium supplemented with both NAA and BA. A significant increase in the number of protruding meristemoid tissues was observed in the liquid medium. The ½ MS liquid medium supplemented with 0.5 mg L –1 NAA and 1.0 mg L –1 BA was successfully used to induce meristemoids of petal tissues transiently transformed by A. tumefaciens strain EHA105 carrying pCAMBIA–1301 via agroinfiltration and the subsequent use of 20 mg L –1 meropenem was effective in eliminating Agrobacterium from the infected explants. The transformed status of the meristemoid tissues was confirmed by GUS expression analysis, indicating that the T-DNA of the vector used had been successfully incorporated into the genome. For the first time, the researchers have demonstrated the potential methodology for direct organogenesis from agroinfiltration-transformed petal tissues of D. Sonia 'Earsakul'.
Avonia, 2018
Hoya deleoniorum, a new species from Mindanao, Philippines, is formally described and the differe... more Hoya deleoniorum, a new species from Mindanao, Philippines, is formally described and the differences from H. cutisporcelana W. Suarez, J. R. Sahagun & Aurigue
are reported.
Journal of Nature Studies, 2013
Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Ph... more Hoya cutis-porcelana Suarez, Sahagun & Aurigue sp. nov., endemic to Samar and Biliran Islands, Philippines is described and illustrated. The foliage may appear similar to those of Hoya camphorifolia and Hoya bicolensis, but its flower does not resemble any known Hoya species to date.
Conference Presentations by Jorge Sahagun
Philippine Alocasias were tissue cultured for conservation and commercial purposes. Explants were... more Philippine Alocasias were tissue cultured for conservation and commercial purposes. Explants were obtained from both tip and axillary buds. Commercial bleach (Zonrox) was used to sanitize tissues. Two (2) mm. terminal buds and 3-5mm. axillary buds were excised and cultured in modified MS medium supplemented with 2 ppm 6-Benzylanimopurine (6-BA) and 2g/l peptone for 2 to 3 weeks followed by subcultures every 3 weeks. Alocasia micholitziana, A. sanderana, A. boyseana, A. heterophylla, A. scalprum, A. wenzelli, A. zebrina and 3 other Philippine species (no taxonomic nomenclature yet pending botanic description) were isolated and grown. The same technique was used on some other commercial varieties of Alocasia successfully. Three months after isolation, regenerated plantlets were ready for planting in plugs or in community pots.
Metabolic engineering of flavonoid biosynthesis provides a very promising opportunity for develop... more Metabolic engineering of flavonoid biosynthesis provides a very promising opportunity for developing new flower colours in orchids. However, molecular basis of genes involved in anthocyanin production needs to be established first for easy manipulation. In preparation to production of yellow flavonoid in orchids, we report here the expression profile of flavanone-3-hydroxylase (DseF3H) gene at different floral developmental stages of Dendrobium Sonia ‘Earsakul’ and the transient RNA silencing of DseF3H, which can be the first step for redirecting anthocyanin production into other pathways such as the aurone biosynthetic pathway. Semi-quantitative RT-PCR analysis revealed that the expression of DseF3H was developmentally increased from the early bud to flower opening while slightly decreased only at the fully opened flower stage. This indicates that F3H is a key gene in anthocyanin production in D. Sonia ‘Earsakul’ flowers. Transient silencing of DseF3H in the floral tissues of D. Sonia ‘Earsakul’ was induced by delivering 261-bp DseF3H hairpin RNA (hpRNA) into the petal and sepal tissues of flower buds at early developmental stage (2.0-2.3 cm.) via agroinfiltration. Down-regulation of endogenous DseF3H mRNA transcript and colour changes were observed in infiltrated areas of petal and sepal tissues as a result of impaired anthocyanin accumulation. Our experiments demonstrate that DseF3H gene suppression through hpRNA-induced silencing system can be utilized for blocking the endogenous anthocyanin production and therefore, a potential tool for redirecting to the yellow aurone biosynthesis in D. Sonia ‘Earsakul’ and other Dendrobium orchid species.
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Papers by Jorge Sahagun
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Conference Presentations by Jorge Sahagun
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