Papers by Kumrop Ratanasut
Philippine Journal of Science, 2018
This study aimed to examine a promising procedure for in vitro direct organogenesis from floral t... more This study aimed to examine a promising procedure for in vitro direct organogenesis from floral tissues of Dendrobium Sonia 'Earsakul' as a new potential regeneration system for transformation in orchids. Petal and sepal explants from floral buds (2.0–2.3 cm) survived on ½ MS solid medium supplemented with or without 1-naphthaleneacetic acid (NAA) or/and benzylaminopurine (BA), but only petal explants generated protruding meristemoid tissues when they were cultured on ½ MS medium supplemented with both NAA and BA. A significant increase in the number of protruding meristemoid tissues was observed in the liquid medium. The ½ MS liquid medium supplemented with 0.5 mg L –1 NAA and 1.0 mg L –1 BA was successfully used to induce meristemoids of petal tissues transiently transformed by A. tumefaciens strain EHA105 carrying pCAMBIA–1301 via agroinfiltration and the subsequent use of 20 mg L –1 meropenem was effective in eliminating Agrobacterium from the infected explants. The transformed status of the meristemoid tissues was confirmed by GUS expression analysis, indicating that the T-DNA of the vector used had been successfully incorporated into the genome. For the first time, the researchers have demonstrated the potential methodology for direct organogenesis from agroinfiltration-transformed petal tissues of D. Sonia 'Earsakul'.
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Papers by Kumrop Ratanasut