Survey of protein engineering strategies (Q56895981)

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Protein stability promotes evolvability

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Catalytic promiscuity in biocatalysis: using old enzymes to form new bonds and follow new pathways

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Directed evolution of an amine oxidase for the preparative deracemisation of cyclic secondary amines

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Enhancement, relaxation, and reversal of the stereoselectivity for phosphotriesterase by rational evolution of active site residues

21 January 2018

1 reference

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Directed evolution of a fungal peroxidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Sitagliptin manufacture: a compelling tale of green chemistry, process intensification, and industrial asymmetric catalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).

21 January 2018

1 reference

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An enzyme library approach to biocatalysis: development of nitrilases for enantioselective production of carboxylic acid derivatives

21 January 2018

1 reference

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Bioinformatics-driven, rational engineering of protein thermostability

21 January 2018

1 reference

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Improving catalytic function by ProSAR-driven enzyme evolution

21 January 2018

1 reference

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21 January 2018

1 reference

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Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations

21 January 2018

1 reference

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Directed enzyme evolution via small and effective neutral drift libraries

21 January 2018

1 reference

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Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate

21 January 2018

1 reference

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De novo computational design of retro-aldol enzymes

21 January 2018

1 reference

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Natural diversity to guide focused directed evolution

21 January 2018

1 reference

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Converting an esterase into an epoxide hydrolase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Thermostabilization of an esterase by alignment-guided focussed directed evolution

21 January 2018

1 reference

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Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration

21 January 2018

1 reference

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Enhancing catalytic promiscuity for biocatalysis

21 January 2018

1 reference

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Finding better protein engineering strategies

21 January 2018

1 reference

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Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

21 January 2018

1 reference

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From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase

21 January 2018

1 reference

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The consensus concept for thermostability engineering of proteins

21 January 2018

1 reference

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Combinatorial alanine substitution enables rapid optimization of cytochrome P450BM3 for selective hydroxylation of large substrates

21 January 2018

1 reference

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Engineering proteinase K using machine learning and synthetic genes

21 January 2018

1 reference

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Novel methods for directed evolution of enzymes: quality, not quantity

21 January 2018

1 reference

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Kinetic stabilization of Bacillus licheniformis alpha-amylase through introduction of hydrophobic residues at the surface

21 January 2018

1 reference

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An S-selective lipase was created by rational redesign and the enantioselectivity increased with temperature

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding

21 January 2018

1 reference

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Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

21 January 2018

1 reference

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Improving enzyme properties: when are closer mutations better?

21 January 2018

1 reference

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Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

21 January 2018

1 reference

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Directed evolution: selecting today's biocatalysts

21 January 2018

1 reference

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Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations

21 January 2018

1 reference

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Improving the Catalytic Activity of Candida antarctica Lipase B by Circular Permutation

21 January 2018

1 reference

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Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes

21 January 2018

1 reference

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Constructing and analyzing the fitness landscape of an experimental evolutionary process

21 January 2018

1 reference

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Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Enhancing the enantioselectivity of an epoxide hydrolase by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space

21 January 2018

1 reference

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Addressing the numbers problem in directed evolution

21 January 2018

1 reference

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Kemp elimination catalysts by computational enzyme design

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Biocatalytic asymmetric synthesis of chiral amines from ketones applied to sitagliptin manufacture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Directed evolution of an esterase from Pseudomonas fluorescens yields a mutant with excellent enantioselectivity and activity for the kinetic resolution of a chiral building block

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Molecular breeding of carotenoid biosynthetic pathways

21 January 2018

1 reference

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Conversion of a PLP-dependent racemase into an aldolase by a single active site mutation

21 January 2018

1 reference

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Selection and evolution of enzymes from a partially randomized non-catalytic scaffold

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

21 January 2018

1 reference

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Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Engineering an enzyme to resist boiling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Evolutionary potential of (beta/alpha)8-barrels: in vitro enhancement of a "new" reaction in the enolase superfamily

21 January 2018

1 reference

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Darwinian evolution can follow only very few mutational paths to fitter proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Engineering delta 9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor delta 9-18:0-ACP desaturase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

A statistical analysis of random mutagenesis methods used for directed protein evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Improvement of the Thermostability and Activity of a Pectate Lyase by Single Amino Acid Substitutions, Using a Strategy Based on Melting-Temperature-Guided Sequence Alignment

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F0471140864.PS2607S66