Crystal structure of HIV-1 Tat complexed with human P-TEFb (Q24634569)

2

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Katayoun Varzavand

3

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Jeffrey J Cooper

4

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Stanley C Sedore

5

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David H Price

The structure of P-TEFb (CDK9/cyclin T1), its complex with flavopiridol and regulation by phosphorylation

6

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language of work or name

English

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publication date

10 June 2010

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published in

Nature

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volume

465

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issue

7299

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page(s)

747-51

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cites work

1 reference

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Inhibition of P-TEFb (CDK9/Cyclin T) kinase and RNA polymerase II transcription by the coordinated actions of HEXIM1 and 7SK snRNA

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MAQ1 and 7SK RNA interact with CDK9/cyclin T complexes in a transcription-dependent manner

7 April 2017

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Identification of multiple cyclin subunits of human P-TEFb

7 April 2017

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Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor bound to the cyclin A-Cdk2 complex

7 April 2017

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Structural and functional characterization of human immunodeficiency virus tat protein

7 April 2017

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Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV

7 April 2017

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Structural basis for transcription elongation by bacterial RNA polymerase

7 April 2017

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A 1.3-A resolution crystal structure of the HIV-1 trans-activation response region RNA stem reveals a metal ion-dependent bulge conformation

7 April 2017

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WebLogo: A Sequence Logo Generator

7 April 2017

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Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination

7 April 2017

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Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription

7 April 2017

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Controlling the elongation phase of transcription with P-TEFb

7 April 2017

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Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186

7 April 2017

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Cyclin K functions as a CDK9 regulatory subunit and participates in RNA polymerase II transcription

7 April 2017

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P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro

7 April 2017

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Transcription elongation factor P-TEFb is required for HIV-1 Tat transactivation in vitro

7 April 2017

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Control of RNA polymerase II elongation potential by a novel carboxyl-terminal domain kinase

7 April 2017

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The remarkable frequency of human immunodeficiency virus type 1 genetic recombination

7 April 2017

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What does the structure-function relationship of the HIV-1 Tat protein teach us about developing an AIDS vaccine?

27 September 2017

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Role of the HIV-1 positive elongation factor P-TEFb and inhibitors thereof

27 September 2017

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Tat acetylation modulates assembly of a viral-host RNA-protein transcription complex

27 September 2017

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RNA-driven cyclin-dependent kinase regulation: when CDK9/cyclin T subunits of P-TEFb meet their ribonucleoprotein partners.

27 September 2017

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Manipulation of P-TEFb control machinery by HIV: recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR.

27 September 2017

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Tat competes with HEXIM1 to increase the active pool of P-TEFb for HIV-1 transcription.

27 September 2017

1 reference

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Global and regional distribution of HIV-1 genetic subtypes and recombinants in 2004.

27 September 2017

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The Yin and Yang of P-TEFb regulation: implications for human immunodeficiency virus gene expression and global control of cell growth and differentiation

27 September 2017

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TAR RNA loop: a scaffold for the assembly of a regulatory switch in HIV replication

27 September 2017

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Flavopiridol inactivates P-TEFb and blocks most RNA polymerase II transcription in vivo

27 September 2017

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Interactions between equine cyclin T1, Tat, and TAR are disrupted by a leucine-to-valine substitution found in human cyclin T1.

27 September 2017

1 reference

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Multifaceted activities of the HIV-1 transactivator of transcription, Tat.

27 September 2017

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Mechanisms of cyclin-dependent kinase regulation: structures of Cdks, their cyclin activators, and Cip and INK4 inhibitors

27 September 2017

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Interactions between Tat and TAR and human immunodeficiency virus replication are facilitated by human cyclin T1 but not cyclins T2a or T2b.

27 September 2017

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Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specificity of HIV-1 Tat.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

The interaction between HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1, and Tat.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

Multiple functional domains of Tat, the trans-activator of HIV-1, defined by mutational analysis

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

Mutational analysis of the conserved cysteine-rich region of the human immunodeficiency virus type 1 Tat protein

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

HIV-1 Tat interacts with cyclin T1 to direct the P-TEFb CTD kinase complex to TAR RNA.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

Conservative mutations in the putative metal-binding region of human immunodeficiency virus tat disrupt virus replication

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

Phosphorylated positive transcription elongation factor b (P-TEFb) is tagged for inhibition through association with 7SK snRNA.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

Mutagenesis of EIAV TAT reveals structural features essential for transcriptional activation and TAR element recognition.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2885016

Virology. Moving forward in HIV vaccine development

28 November 2018

1 reference

12 December 2020

Processing of X-ray diffraction data collected in oscillation mode

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12 December 2020

Specific versus non-specific contacts in protein crystals

1 reference

12 December 2020

High-Resolution Crystals of Methionine Aminopeptidase fromPyrococcus furiosusObtained by Water-Mediated Transformation

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12 December 2020

Identifiers

DOI

10.1038/NATURE09131

1 reference

Dimensions Publication ID

1040895886

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1 reference

1 reference

PubMed publication ID

20535204

1 reference