Research PPT - Dtu

ANUPAM
A
AWADHES
H PANDEY
TITLE
Investigating Novel Plastic-

Degrading Bacteria From
Dumped Soil Areas for
Sustainable Plastic Waste
Management
INTRODUCTION
 Plastic has become an indispensable part of our lives and cutting down
plastic consumption entirely is difficult to achieve.
 The recalcitrant and non-biodegradable nature of plastic leads to
accumulation of tons of plastic in landfills and water bodies which further risks
marine life and human life too causing serious health issues.
 Plastic pollution is a global environmental crisis, with millions of tons of plastic
waste generated annually.
 Traditional methods of plastic disposal are inadequate, leading to an urgent need for
innovative solutions.
 Recent studies have identified specific bacteria capable of degrading plastics,
presenting a promising avenue for bioremediation.
 Understanding the genetic and metabolic pathways involved in plastic
degradation
is crucial for bioremediation strategies.
 This research aims to explore novel plastic-degrading bacteria, characterize their
enzymatic mechanisms, and assess their potential for practical applications in waste
management.
REVIEW OF LITERATURE
 The literature on plastic-degrading bacteria in waste management highlights
the urgent need for advancements in plastic waste management due to the
significant environmental concerns posed by plastic litter
accumulation (Chigwada & Tekere, 2023).
 Additionally, microbial consortia or cocultures are recognized as cost-

effective and eco-friendly alternatives to conventional plastic waste
management methods, with the potential to convert by-products of microbial
degradation into bio-energy sources (Skariyachan et al., 2021).
 Contradictorily, while bacterial degradation is deemed the most effective

biodegradation approach, our understanding of the mechanisms and strategies
to enhance this process remains limited (He et al., 2023).
 Moreover, despite the identification of microorganisms capable of degrading plastic
waste, the implementation of these biological treatment strategies on a commercial scale is still
in its infancy (Fernández et al., 2022; Shah, 2020).
 However, further research is necessary to optimize these biological processes and translate
them into viable commercial applications (Fernández et al., 2022; He et al., 2023).
 The future of plastic waste management is likely to be shaped by advancements in our

understanding of microbial degradation mechanisms and the strategic enhancement of
biodegradation capabilities (He et al., 2023; Yang et al., 2023).
 An excellent but perhaps overlooked tool for the study of microbial activity in the soil,
nutrient cycling, microbial succession and ecology is the Winogradsky column (Pigage et al.,
1985).
AIMS AND OBJECTIVES
 Isolation and Characterization: Isolate novel plastic-degrading
bacteria from contaminated environments thriving in both aerobic and
anaerobic conditions.
 Enzymatic Analysis: Characterize the enzymes involved in plastic

degradation.
 Metabolic Pathway Elucidation: Investigate metabolic pathways

associated with plastic degradation.
 Bioremediation Potential: Evaluate the effectiveness of isolated

bacteria in real-world plastic waste scenarios.
METHODOLOGY
1. Sample Collection
▶ Locations: Collect soil and water samples from plastic-polluted sites, such as
landfills, rivers, and coastal areas.
2. Isolation of Bacteria
▶ Serial Dilution
▶ Spread Plate Technique
3. Screening for Degradation Activity

• Agar cup Method
• Clear zone Method
• Streak
Method
4. Winogradsky Column
Evaluate bacterial growth on plastic substrates and measure degradation rates
using weight loss and visual inspection over time under anaerobic conditions.
5. Identification
• Morphological: i) Gram Staining
ii) Colony Characteristics
• Biochemical Test
6. Enzymatic Characterization
▶ Enzyme Assays: Identify and characterize enzymes involved in plastic degradation through
biochemical assays.
▶ Molecular Techniques: Perform gene sequencing and analysis to identify key plastic-
degrading enzymes.
7. Metabolic Pathway Analysis
▶ Bioinformatics Tools: Utilize genomic and transcriptomic data to reconstruct metabolic

pathways involved in plastic degradation using databases like KEGG and MetaCyc.
 Expected Outcomes
▶ Identification of novel plastic-degrading bacteria with high degradation efficiency.

▶ Characterization of key enzymes and metabolic pathways involved in plastic breakdown.
▶ Practical recommendations for using these bacteria in bioremediation strategies for plastic
waste management.
 Significance of the Study
▶ This research will contribute significantly to the field of environmental microbiology and
bioremediation by providing insights into effective biological solutions for plastic pollution.
The findings could lead to the development of sustainable practices for managing plastic
waste and restoring contaminated environments.
CONCLUSION
▶ This research proposal outlines a comprehensive approach to

investigating plastic-degrading bacteria, aiming to contribute
valuable insights and practical solutions to the plastic pollution
crisis. Through isolation, characterization, and application of these
microorganisms, we hope to advance the field of environmental
biotechnology and promote sustainable waste management
practices.
▶ Recent advancements in the study of plastic-degrading bacteria

demonstrate significant progress in identifying new species,
characterizing their enzymes, and applying this knowledge to
practical solutions for plastic pollution. Continued research in this
field is crucial for developing sustainable strategies to combat
plastic waste effectively.
REFRENCES
▶ Yoshida, S., et al. (2016). "A bacterium that degrades and assimilates poly(ethylene
terephthalate). Science.“
▶ Ryu, H. W., et al. (2018). "Biodegradation of plastics by Pseudomonas."
Environmental Microbiology Reports.
▶ Kawai, F., et al. (2014). "Isolation of bacteria capable of degrading polyurethane."
Applied Microbiology and Biotechnology
▶ Hadad, D., Geresh, S., & Sivan, A. (2005). Biodegradation of polyethylene by the
thermophilic bacterium Brevibacillusborstelensis. Journal of applied microbiology,
98(5), 1093-1100. 4.
▶ Russell JR, Huang J, Anand P, Kucera K, Sandoval AG, Dantzler KW,
Hickman D, Jee J, Kimovec FM, Koppstein D, Marks DH,( 2011), Biodegradation
of polyester polyurethane by endophytic fungi. Appl Environ Microbiol., 77(17):
6076-6084
THANK YOU

Research PPT - Dtu

Uploaded by

Copyright:

Available Formats

Research PPT - Dtu

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Research PPT - Dtu

Uploaded by

Copyright:

Available Formats

ANUPAM

Investigating Novel Plastic-

 Additionally, microbial consortia or cocultures are recognized as cost-

 Contradictorily, while bacterial degradation is deemed the most effective

 The future of plastic waste management is likely to be shaped by advancements in our

 Enzymatic Analysis: Characterize the enzymes involved in plastic

 Metabolic Pathway Elucidation: Investigate metabolic pathways

 Bioremediation Potential: Evaluate the effectiveness of isolated

3. Screening for Degradation Activity

7. Metabolic Pathway Analysis

▶ Bioinformatics Tools: Utilize genomic and transcriptomic data to reconstruct metabolic

▶ Identification of novel plastic-degrading bacteria with high degradation efficiency.

 Significance of the Study

▶ This research proposal outlines a comprehensive approach to

▶ Recent advancements in the study of plastic-degrading bacteria

You might also like