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    Anticancer Effects of The Combined Thai Noni Juice

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    Muhammad Ali Khumaini

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    © All Rights Reserved
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    Anticancer Effects of The Combined Thai Noni Juice

    Uploaded by

    Muhammad Ali Khumaini
    58 views14 pages

    Original Title

    Anticancer Effects of the Combined Thai Noni Juice

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    Copyright:

    © All Rights Reserved
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    Download as pptx, pdf, or txt
    58 views14 pages

    Anticancer Effects of The Combined Thai Noni Juice

    Uploaded by

    Muhammad Ali Khumaini

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 14

    Anticancer effects of the combined Thai noni juice

    ethanolic extracts and 5‐fluorouracil against


    cholangiocarcinoma cells in vitro and in vivo
    Prompipak et al., 2021

    Muh. Ali Khumaini M.B.


    6110730013 1
    Introduction
    Treatment
    • Surgical resection (early stage)
    - Less likely to be diagnosed.

    • Chemoteraphy (advanced stage)


    - 5-fluorouracil (5-FU), cisplatin (CIS), and gemcitabine
    (GEM) 5.

    2
    Objectives

    Thai noni juice cholangiocarcinoma cells


    extract

    5‐fluorouracil
    nude mouse xenograft model 3
    Methods

    1. Cell culture 2. Thai noni juice extract

    Cell line Name Medium Thai noni juice Chiangrai (TNJ-


    Cr) and Buasri (TNJ-Bs)
    cholangiocarcinoma KKU-100 RPMI-1640
    cholangiocarcinoma KKU-213B RPMI-1640
    normal human H69 DMEM
    cholangiocyte
    Extracted with absolute ethanol
    (7:3) and the extract freeze dried

    4
    3. Cell viability assay 4. Cell cycle analysis

    8 × 103 cells/well 1 × 106 cells cultured for 24 h

    Single agent or combination of Single agent or combination


    5-FU & TNJ (synergistic) of 5-FU & TNJ (48
    h)

    24, 48, and 72 h incubation Cells harvested & washed with


    cold PBS + 1% glucose and Flow cytometer
    fixed in 70% ethanol for 1 h on
    2 h incubation with ice
    medium+MTT
    washed with PBS then incubated Stained through incubation with
    in RNase A 30 min Propidium Iodide (45 min)
    Added DMSO and measure
    absorbance

    5
    5. Apoptosis detection by flow cytometry

    1 × 106 cells cultured for 24 h

    Single agent or combination


    (synergistic) of 5-FU & TNJ (48
    h)

    Cells harvested & washed with


    cold PBS Flow cytometer

    Resuspended in Annexin V- Stained with annexin V-FITC


    binding buffer and PI (15 min)

    6
    6. Anti‑CCA in nude mice xenograft
    model
    i.p. administration single agent
    Tumor or combination (synergistic) of
    5-FU & TNJ (14 days)

    Organ anatomy and morphology


    + % tumor inhibition

    administered subcutaneously into the right axillary


    with KKU-100 cells (2 × 106 cells in 0.1 mL of
    serum-free medium mixed with Matrigel)

    7
    Results
    1. Antiproliferative effects of
    the single agent treatment

    8
    2. Antiproliferative effects of
    the combination treatment

    9
    3. Effects on cell cycle distribution

    10
    4. Effects on cell apoptosis induction

    11
    5. Antitumor effect on CCA xenograft mice

    12
    5. Histological examonatio on CCA xenograft mice

    13
    Conclusion
    Combination treatments of 5-FU and TNJ-Cr or TNJ-Bs ethanolic extracts
    showed the synergistic antitumor effects on the human CCA cell lines, KKU-
    100 and KKU-213B. Moreover, the combined treatment of 5-FU and TNJ-Bs
    ethanolic extract was not toxic to the non-cancer H69 cells in vitro.
    Additionally, TNJ ethanolic extracts enhanced the antitumor growth of 5-FU
    on the mouse KKU-100 xenograft model through apoptosis induction.

    14

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