Chapter 5 - Cell Structure

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Cell Structure

Chapter 5

c
Cell Theory

‡ All organisms are composed of one or more


cells.
‡ Cells are the smallest living units of all living
organisms.
‡ Cells arise only by division of a previously
existing cell.

a
Cell Characteristics
‡ Genetic material
± single circular molecule of DNA in

prokaryotes
± double helix located in nucleus in

eukaryotes ± nuclear envelope (double


membrane
‡ Cytoplasm fills cell interior ±
ð  
± sugars, amino acids,

± proteins - organelles

‡ Plasma membrane encloses


± the cell ± phospholipid bilayer


  
˜
Generalized Eukaryotic Cell

^
Cell Size

‡ Most cells are relatively small because as


size increases, volume increases much
more rapidly.
± longer diffusion time

÷
ºisualizing Cells

‡ Resolution - minimum distance two points


can be apart and still be distinguished as two
separate points
± Compound microscopes - magnify in

stages using multiple lenses


± Transmission electron microscope -

electrons transmitted through specimen


± Scanning electron microscope - electrons

beamed onto surface of the specimen

£
ºisualizing Cells

ÿ
Prokaryotic Cells
‡ Simplest organisms
± Cytoplasm is surrounded by plasma membrane and

encased in a rigid cell wall composed of


peptidoglycan.
D no distinct interior compartments

gram-positive ± thick single layer wall that


retains a violet dye from Gram stain procedure
gram-negative ± multilayered wall does not
retain dye
 Susceptibility of bacteria to antibiotics

depends on cell wall structure.



Prokaryotic Cells

‡ Some use flagellum for locomotion


± threadlike structures protruding from cell

surface      

 


    

‰
Eukaryotic Cells

‡ Characterized by compartmentalization by
an endomembrane system, and the
presence of membrane-bound organelles.
± Central vacuole ± plants, storage

± ºesicles (smaller)

± Chromosomes - DNA and protein

± Cytoskeleton (internal protein scaffolding)

± Cell walls ± plants and fungi

c
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ca
Nucleus
‡ Repository for genetic material
‡ Directs activities of the cell
‡ Usually single, some cells several, RBC none
± Nucleolus - region of intensive ribosomal

RNA synthesis
‡ Surface of nucleus bound by two phospholipid
bilayer membranes
± nuclear membrane

± Nuclear pores ± protein gatekeepers

D Usually proteins going in and RNA going

out

Nucleus

c^
Chromosomes

‡ DNA of eukaryotes is divided into linear


chromosomes.
± exist as strands of chromatin, except

during cell division


± associated with packaging histones,

packaging proteins
D nucleosomes


Endomembrane System
‡ Compartmentalizes cell, channeling passage
of molecules through cell¶s interior.
± Endoplasmic reticulum

D Rough ER - studded with ribosomes

D Smooth ER - few ribosomes


Endoplasmic reticulum
‡ Largest internal membrane
‡ Composed of Lipid bilayer
‡ Serves as system of channels from the
nucleus
‡ Functions in storage and secretion
‡ Rough ER is ³rough´ because of associated
ribosomes (sites of protein synthesis
‡ Smooth ER - lack associated ribosomes ±
contained embedded enzymes, catalyze
synthesis of carbohydrate and lipid
molecules
cÿ
Endomembrane System
‡ Golgi apparatus
± collection of Golgi bodies
D collect, package, and distribute molecules

synthesized at one location in the cell and


utilized at another location
D Front - m , Back ± 

D Cisternae ± stacked membrane folds

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Endomembrane System
‡ ºesicles
± Lysosomes - membrane-bound vesicles

containing digestive enzymes ± from Golgi


± Microbodies - enzyme-bearing,

membrane-enclosed vesicles.
D Peroxisomes - contain enzymes that

catalyze the removal of electrons and


associated hydrogen atoms
D Peroxisome ± named for hydrogen

peroxide produced as a by-product


D Enzyme breaks down to water and

oxygen a
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Ribosomes

‡ Ribosomes are RNA-protein complexes


composed of two subunits that join and
attach to messenger RNA.
± site of protein synthesis

± assembled in nucleoli

aa
ërganelles With DNA

‡ Mitochondria
± bounded by exterior and interior

membranes
± interior partitioned by cristae

‡ Chloroplasts
± have enclosed internal compartments of

stacked grana, containing thylakoids


± found in photosynthetic organisms


Mitochondria
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a^
Chloroplasts


      



    
 

   
     

   


  


   



Endosymbiosis

‡ Endosymbiotic theory suggests engulfed


prokaryotes provided hosts with advantages
associated with specialized metabolic
activities.


Theory of Endosymbiosis

aÿ
'    
    
        V

- Are appropriate size to be descendants of eubacteria.


- Have inner membranes similar to those on prokaryotic
plasma membranes.
- Replicate by splitting, as in prokaryotes.
- DNA is circular and different from the DNA of the cell's
nucleus.
- Contain their own components for DNA transcription and
translation into proteins .
- Have ribosomes similar to prokaryotic ribosomes.
- Molecular systematics lend evidence to support this
theory.
- Many extant organisms are involved in endosymbiotic
relationships.
a
Cytoskeleton
‡ Network of protein fibers supporting cell shape and
anchoring organelles
± Actin filaments

D cell movement



± Microtubules

 

D Hollow tubes  

D Facilitate cell movement

D Centrioles ± barrel shaped

D organelles occur in pairs ±

D help assemble animal cell¶s microtubules


O

± Intermediate filaments

D Stable - don¶t break down


Cytoskeleton

˜
Plant Cells

‡ Central vacuole
± often found in the center of a plant, and

serves as a storage facility for water and


other materials
‡ Cell wall
± primary walls ± laid down while cell is

growing
± middle lamella ± glues cells together

± secondary walls ± inside the primary cell

walls after growth


˜c
Plant Cell

˜a
Animal Cells

‡ Animal cells lack cell walls.


± form extracellular matrix

D provides support, strength, and resilience

˜˜
˜^
˜÷
˜£

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