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    Presentation ON: Fluorescence

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    Bandita Datta
    The document presents a presentation on fluorescence done by Suman Chhetri. It contains sections on how fluorescence works involving the absorption and re-emission of light, the Jablonski diagram depicting electronic transitions, differences between fluorescence and phosphorescence, examples of biofluorescence and bioluminescence in nature, naturally fluorescent molecules such as proteins and pigments, and applications of fluorescence including fluorescent lighting and fluorescence spectroscopy.

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    Presentation ON: Fluorescence

    Uploaded by

    Bandita Datta
    403 views14 pages
    The document presents a presentation on fluorescence done by Suman Chhetri. It contains sections on how fluorescence works involving the absorption and re-emission of light, the Jablonski diagram depicting electronic transitions, differences between fluorescence and phosphorescence, examples of biofluorescence and bioluminescence in nature, naturally fluorescent molecules such as proteins and pigments, and applications of fluorescence including fluorescent lighting and fluorescence spectroscopy.

    Original Description:

    Properties of flourescence

    Original Title

    Fluorescence

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    The document presents a presentation on fluorescence done by Suman Chhetri. It contains sections on how fluorescence works involving the absorption and re-emission of light, the Jablonski diagram depicting electronic transitions, differences between fluorescence and phosphorescence, examples of biofluorescence and bioluminescence in nature, naturally fluorescent molecules such as proteins and pigments, and applications of fluorescence including fluorescent lighting and fluorescence spectroscopy.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    403 views14 pages

    Presentation ON: Fluorescence

    Uploaded by

    Bandita Datta
    The document presents a presentation on fluorescence done by Suman Chhetri. It contains sections on how fluorescence works involving the absorption and re-emission of light, the Jablonski diagram depicting electronic transitions, differences between fluorescence and phosphorescence, examples of biofluorescence and bioluminescence in nature, naturally fluorescent molecules such as proteins and pigments, and applications of fluorescence including fluorescent lighting and fluorescence spectroscopy.

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    At a glance
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    The key takeaways are that fluorescence involves the absorption and re-emission of light at a lower energy level. It occurs rapidly and stops when the light source is removed, while phosphorescence involves transitions from triplet states and persists slightly after removal of the light source.

    Fluorescence is the emission of light from a substance that has absorbed light or other electromagnetic radiation of a higher frequency. It is a form of luminescence and involves electronic excitation from light absorption.

    Fluorescence occurs rapidly when molecules drop from singlet excited states to the ground state, while phosphorescence also involves triplet states, has a longer lifetime, and persists slightly after removal of the light source. The energy released is also lower in phosphorescence compared to fluorescence.

    PRESENTATION ON

    FLUORESCENCE

    DONE BY:
    SUMAN CHHETRI

    CONTENTS:
    INTRODUCTION
    HOW

    FLUORESCENCE WORKS

    HOW

    FLUORESCENCE CAN BE SEEN

    JABLONSKI

    DIAGRAM

    DIFFERENCES

    BETWEEN PHOSPHORESCENCE
    AND FLUORESCENCE

    LUMINESCENCE
    BIOFLUORESCENCE
    BIOLUMINESCENCE
    NATURALLY

    FLUORESCENT MOLECULES

    APPLICATIONS

    What is fluorescence?
    Fluorescenceisthepropertyofemittingelectromagnetic
    radiationintheformoflightastheresultof(andonly
    during)theabsorptionoflightfromanothersource.
    Importantcharacteristicsoffluorescence:
    1.Itistheresultoftheabsorptionoflight.
    2.Itoccursduringabsorptiononly.
    3.Itinvolvestheemissionoflight.
    4.Anoutsidesourceofenergyisrequired.

    Energetic Transitions of Electrons


    During Fluorescence

    Lifetimeofelectronsinexcitedstateisshort:1013
    (absorption)and109s(emission).

    HOW FLUORESCENCE WORKS

    Dual nature of light.

    Photon(packet of energy)comes into contact with an electron.


    Thereby the electron absorb energy and jumps into a higher
    energy level.

    But it stay there for some period and come back to lower energy
    level with release of energy.

    If its a photon in uv region striking an atom, and if the atom


    emits a visible photon in return, the material is UV sensitive. This
    is the glow effect associated with black light.

    HOW FLUORESCENCE CAN BE SEEN

    Jablonski Energy Diagram


    S2, S1 = Singlet States
    T1 = Triplet State

    Numerous vibrational energy levels for each electronic state

    Resonance Radiation - reemission at same


    usually reemission at higher (lower energy)
    Forbidden transition: no direct excitation of triplet state
    because change in multiplicity selection rules.

    DIFFERENCES BETWEEN PHOSPHORESCENCE


    AND FLUORESCENCE

    FLUORESCENCE

    PHOSPHORESCENCE

    Fluorescence stops as soon as we take


    away the light source .

    Phosphorescence tend to stay little


    longer even after irradiating is
    removed.

    Fluorescence takes place when excited


    energy is released and the molecule
    comes back to ground state from the
    singlet state.
    The energy released in fluorescence is
    higher.

    Phosphorescence takes place when a


    molecule is coming back to the ground
    state from the triplet state.
    The energy released in
    phosphorescence is lesser than
    fluorescence.

    In fluorescence , the absorbed amount In phosphorescence, the released


    of energy is released back.
    amount of energy is lower than what is
    absorbed.

    LUMINESCENCE

    Matter is luminescent if it gives off energy in the form of


    light.

    Chemiluminescence is light which results from a chemical


    reaction

    The light emitted in such reactions has differing degrees


    of intensity and lifetime.

    FLUORESCENCE IN NATURE

    BIOFLUORESCENCE

    It is absorption of electromagnetic wavelengths from the visible light


    spectrum by fluorescent proteins in living organism and reemission of that
    light at lower energy level.

    The energized electrons returned to a stable state with release of excess


    energy in the form of fluorescent light.

    Jellyfish is the one of the example of Biofluorescence.

    BIOLUMINESCENCE

    It is the production and emission of light by a living organism.

    It is different from biofluorescence is that it is natural


    production of light by chemical reactions within an organism.

    The chemical reaction in bioluminescence involves the light


    emitting pigment and enzyme ,assisted by other proteins.

    Naturally Fluorescent Molecules


    Proteins:
    Heme proteins (hemoglobin, myoglobin, cytochrome C,
    hemocyanin)
    Redox & photo proteins (phytochromes, phycoerythrin,
    phycocyanin, green fluorescent protein, phycobiliproteins,
    rhodopsin, ferredoxins)

    Pigments:
    flavins, stilbenes, tryptophan, tyrosine, purines..
    Minerals, metallic chelates

    APPLICATIONS

    Fluorescence is used in the fluorescent tubes used for light


    purposes.

    The presence of invisible ultraviolet rays can be detected


    by their fluorescent effect.

    Fluorescent proteins in corals may contribute to


    photosynthesis by converting unusable wavelength of
    light .

    Fluorescence spectroscopy.

    It is used in signage , forensics and optical brightener.

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