Fluoroskan FL PDF

Thermo Scientific Fluoroskan Ascent FL
The Thermo Scientific Fluoroskan Ascent FL is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, and immuno and cell based assays. The Fluoroskan Ascent FL is a compact and robust instrument with excellent optical performance for a variety of fluorometric and luminometric research applications, offering versatile plate formats, fast reading speeds, up to three dispensers and top/bottom reading of plates.
Fast reading speed The fast reading time, just 15 seconds for a 96-well plate, is essential for kinetic applications, such as enzyme kinetics and phagocytosis assays. Even the combination of a fluorometric GFP measurement and a luminometric luciferase measurement from the same well is fast due to the versatility of assay programming in the same protocol. Onboard dispensers for exact follow-up of kinetic reactions Equipped with up to three onboard reagent dispensers, the Fluoroskan Ascent FL enables fast kinetic assays, such as Ca2+ flux and ATP assays. The instrument supports simultaneous dispensing and reading, enabling monitoring of fast
kinetic measurements from the very start of the reaction. A very low dead volume and the backflush capability of the dispensers are important issues when using expensive reagents. High sensitivity for both top and bottom reading Fiberless direct illumination optics for both top and bottom reading ensures a high sensitivity, a wide dynamic range, low crosstalk and accurate and precise results in both luminometric and fluorometric assays. Specially designed for automation To increase assay throughput, the Fluoroskan Ascent FL can read up to 384-well plates and easily be integrated with automated systems.
Fluoroskan Ascent FL Applications: Ca2+ flux assays Cell proliferation Cytotoxicity Cell adhesion DNA quantitation Reporter gene assays Hybridization assays Quantitation of PCR products FRET assays BRET assays Molecular beacon assays Immunoassays Enzyme activity Bacterial quantitation Phagocytosis Oligonucleotide assays
A compact and robust microplate reader with excellent optical performance for a variety of fluorometric and luminometric research applications.
Compatible with Thermo Scientific RapidStak
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Detection Instruments | Fluoroskan Ascent FL
Technical Specifications and Ordering Information

Thermo Scientific Fluoroskan Ascent, Luminoskan Ascent and Fluoroskan Ascent FL Fluoroskan Ascent Fluorometry Excitation wavelength range Emission wavelength range Excitation filters 320 - 700 nm 360 - 800 nm Up to eight filters in the excitation filter wheel. 355 nm and 485 nm filters included as standard. Other filters available upon request. Up to eight filters in the emission filter wheel. 460 nm and 538 nm filters included as standard. Other filters available upon request. 2 fmol fluorescein/well in a black 96-well plate > 6 decades Luminoskan Ascent Fluoroskan Ascent FL 320 - 700 nm 360 - 670 nm Up to eight filters in the excitation filter wheel. 355 nm and 485 nm filters included as standard. Other filters available upon request. Up to six filters in the emission filter wheel. 460 nm and 538 nm filters included as standard. Other filters available upon request. 2 fmol fluorescein/well in a black 96-well plate > 6 decades 270 - 670 nm 40 amol ATP/well using flash reaction, white 384well plate > 9 decades over whole gain setting area Up to 3 1 - 1000 l in 1 l increments 25 s, 96-well plate, 5 l/well 1 - 384-well plates 15 s, 96-well plate Filters Quartz-halogen lamp Photomultiplier tube From ambient + 3C to 45C, at ambient 25C Orbital shaker Requires , but does not include a personal computer Serial RS-232C port 340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in. options included Basic unit 21 kg (46 lbs.). 3 optional dispensers add 3.5 kg to the weight
Emission filters
Up to six filters in the filter wheel. Filters available upon request.
Sensitivity Dynamic range Luminometry Spectral range Sensitivity Dynamic range Dispensing No of dispensers Dispensing volume Dispensing speed General Features Plate types Measurement speed Wavelength selection Light source Detector Incubator Shaking User interface Computer interface Dimensions (H x W x D)
270 - 670 nm 10 amol ATP/well using flash reaction, white 384-well plate > 9 decades over whole gain setting area Up to 3 1 - 1000 l in 1 l increments 25 s, 96-well plate, 5 l/well 1 - 384-well plates 15 s, 96-well plate Filters Quartz-halogen lamp Photomultiplier tube From ambient + 3C to 45C, at ambient 25C Orbital shaker Requires , but does not include a personal computer Serial RS-232C port 340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in. options included Basic unit 21 kg (46 lbs.). 3 optional dispensers add 3.5 kg to the weight Up to 3 1 - 1000 l in 1 l increments 25 s, 96-well plate, 5 l/well 1 - 384-well plates 15 s, 96-well plate Filters Photomultiplier tube From ambient + 3C to 45C, at ambient 25C Orbital shaker Requires , but does not include a personal computer Serial RS-232C port 340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in. options included Basic unit 21 kg (46 lbs.). 3 optional dispensers add 3.5 kg to the weight
Weight Ordering Information
Cat. No Description Thermo Scientific Fluoroskan Ascent 5210470 Fluoroskan Ascent 100 - 240 V, 50/60 Hz *) 5210480 Fluoroskan Ascent 100 - 240 V, 50/60 Hz, with one dispenser *) 5210482 Fluoroskan Ascent 100 - 240 V, 50/60 Hz, with two dispensers *) 5210483 Fluoroskan Ascent 100 - 240 V, 50/60 Hz, with three dispensers *) *) Includes PC Software and filter pairs: Ex 355 nm / Em 460 nm, Ex 485 nm / Em 538 nm. Other filters available upon request. Thermo Scientific Luminoskan Ascent 5300160 Luminoskan Ascent 100 - 240 V, 50/60 Hz**) 5300170 Luminoskan Ascent 100 - 240 V, 50/60 Hz, with one dispenser**) 5300172 Luminoskan Ascent 100 - 240 V, 50/60 Hz, with two dispensers**) 5300173 Luminoskan Ascent 100 - 240 V, 50/60 Hz, with three dispensers**)
**) Includes PC Software
Thermo Scientific Fluoroskan Ascent FL 5210450 Fluoroskan Ascent FL 100 - 240 V, 50/60 Hz ***) 5210460 Fluoroskan Ascent FL 100 - 240 V, 50/60 Hz with one dispenser***) 5210462 Fluoroskan Ascent FL 100 - 240 V, 50/60 Hz with two dispensers***) 5210463 Fluoroskan Ascent FL 100 - 240 V, 50/60 Hz, with three dispensers ***) ) *** Includes PC Software and filter pairs: Ex 355 nm / Em 460 nm, Ex 485 nm / Em 538 nm. Other filters available upon request.
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Detection Instruments | Ordering Information
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Thermo Scientific Fluoroskan Ascent FL

Compatible with Thermo Scientific RapidStak

Detection Instruments | Fluoroskan Ascent FL

Technical Specifications and Ordering Information

Up to six filters in the filter wheel. Filters available upon request.

Weight Ordering Information

Detection Instruments | Ordering Information

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