User Manual

Binocular Compound Microscope

Model: M82ES Series

MicroscopeNet.com

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Table of Contents

i. Caution..1 ii. Care and Maintenance...2 1. Component Illustration...3 2. Installation.......4 3. Operation........5 4. Specifications......8 5. Optional Parts........9 6. Troubleshooting Guide...............................................................10 7. Dry Darkfield Condenser Installation & Operation Instruction.11

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i. Caution
1. Open the carton carefully with a knife or paper cutter. Find the UP sign and place the Styrofoam container on the side that makes the arrow upward. If the UP sign is missing, please open the Styrofoam container gently to prevent any accessory, i.e. objectives or eyepieces, from dropping and being damaged. 2. Do not discard the molded Styrofoam container; the container should be retained should the microscope ever requires reshipment. 3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty environments. Ensure the microscope is located on a smooth, level and firm surface. 4. If any specimen solutions or other liquids splash onto the stage, objective or any other component, disconnect the power cord immediately and wipe up the spillage. Otherwise, the instrument may be damaged. 5. All electrical connectors (power cord) should be inserted into an electrical surge suppressor to prevent damage due to voltage fluctuations. 6. Confirm that the input voltage indicated on your microscope corresponds to your line voltage. The use of a different input voltage other than that as indicated will cause severe damage to the microscope.

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ii. Care and Maintenance

1. Do not attempt to disassemble any component including eyepieces, objectives or focusing assembly. 2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal surfaces should be cleaned with a damp cloth. More persistent dirt should be removed using a mild soap solution. Do not use organic solvents for cleansing. 3. The outer surface of the optics should be inspected and cleaned periodically using an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth or cotton swab dampened with a lens cleaning solution (available at camera stores). All optical lenses should be swabbed using a circular motion. A small amount of absorbent cotton wound on the end of a tapered stick makes a useful tool for cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up grease making cleaning more difficult. Oil immersion objectives should be cleaned immediately after use by removing the oil with lens tissue or a clean, soft cloth. 4. Observe the specimen with the 4X, 10X and 40X objectives in order, then observe the specimen with the 100X objective. Apply the immersion oil on the slide cover with the 100X objective. Do not let the immersion oil to contact with the dry objectives lens (especially the 40X). Clean the dry objective lens using the camera cleaning kit if the immersion oil is on the dry objectives lens. Clean the 100X objective lens first using the camera cleaning kit after observing the specimen with the 100X objective, then clean the specimen. More persistent dirt should be removed using a little bit alcohol. Do not use organic solvents for cleansing. 5. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover when not in use.

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1 Components Illustration
1 2 13 3 4 5 6 7 8 18 9 10 20 11 21 19 14 15 16 17 12

22 23

24 25

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1 2 3 4 5 6 7 8 9 10 11 12 13

Eyepiece WF10X Diopter Adjusting Ring Eyepiece Tube Nosepiece Objective Slide Holder Mechanical Stage Light Collector Microscope Base Eyepiece WF20X Color Filters Viewing Head Microscope Body -3-

14 15 16 17 18 19 20 21 22 23 24 25 26

Focusing Rack Stop Screw Coarse Focus Knob Fine Focus Knob X-Y Stage Moving Knobs Power Switch Brightness Intensity Dial Immersion Oil Dust Cover Condenser Lock Thumb Screw Condenser Control Knob Condenser Color Filter Holder Iris Diaphragm Lever

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2 Installation
2.1 Installation of the binocular viewing head Head Lock Thumb Screw 1) Loosen the head lock thumb screw (Fig. 1) on the top of the microscope body. Note: The thumb screw may not be pre-installed. Please find it in the same package of the microscope. 2) Remove the cap on the circular dovetail of the binocular viewing head. 3) Insert the binocular viewing head into the top of the microscope body; ensure that the dovetail is completely seated into the socket and tighten the thumb screw. Caution: Fig. 1 Do not release the viewing head from your hand grip until you are sure the viewing head is installed securely. 2.2 Installation of the eyepieces 1) Remove the protective caps from the eyepiece tubes. 2) Insert the eyepieces into the eyepiece tubes. 2.3 Installation of the glass filter 1) Swing out the color filter holder under the condenser. 2) Place the filter into the holder as shown in Fig. 2, swing the holder in.

Color Filter

Color Filter Holder

Fig. 2

2.4 Installation of the camera (optional, may not included in your package) 1) Take off the eyepiece from the eyepiece tube. Camera 2) Insert the camera into the eyepiece tube, and then connect the camera to a computer via USB2.0 cable. See Fig. 3. 3) The manual for the camera is on a CD (or mini CD). Refer to the manual to install the driver and software on to the computer. 4) The camera is optional and may have different color and shape from the one in the figure, depending on the Fig. 3
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www.microscopenet.com model purchased. 2.5 Installation of the dry darkfield condenser (optional, may not included in your
package)

See section 7 (Dry Darkfield Condenser Installation & Operation Instruction)

3 Operation
3.1 Adjusting illumination 1) Plug the power cord into the power socket on the microscope and connect it to the power outlet. 2) Turn on the power switch. 3) Rotate the brightness intensity dial to increase or decrease the brightness. Caution: A diffusion filter is attached beneath the condenser to get uniform light and protect your eyes from strong LED light when a low power objective applies. The diffusion filter can be swung out to make the view field brighter when observing with a high power objective, such as 100X objective. 3.2 Placing specimen 1) Place the slide on the mechanical stage. 2) Use the slide holder to gently secure the slide. 3) Turn the X and Y stage moving knobs to position the specimen in the center of viewing field. Caution: Be sure not to allow an objective to touch a specimen slide when changing objectives. 3.3 Adjusting interpupillary distance While observing with both eyes, hold the left and right eyepiece tubes and slide the tubes in and out. The interpupillary distance is correct when the left and right fields of view converge completely into one image. 3.4 Adjusting eyepiece diopter 1) Rotate the 10x objective into position. 2) Rotate the diopter ring on the right eyepiece tube until its numerical value is the same as your interpupillary distance, for example, 70 in the right figure (See Fig. 4). 3) Close your left eye and bring the specimen into focus following the focusing procedures in 3.5. 4) Close your right eye and bring the same specimen into clear sharp focus by adjusting the diopter ring on left eyepiece tube only. Do not use focus knobs at this step. 5) Since both sides are adjustable, you may also do the above in the opposite way, in 70 other words, left eye first and right eye
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Fig. 4

www.microscopenet.com second. 3.5 Focusing 1) With the 10x objective in position, raise the mechanical stage using the coarse focus knob until the specimen is close to the objective Focusing Rack Stop Screw 2) Turn the coarse focus knob until the specimen is in focus. Use the fine focus knob to obtain a sharp image. You may now switch to another magnification objective. 3) To get a good focused image, you may need to combine the focus knob adjustment and interpupillary distance adjustment, along with eyepiece diopter adjustment stated in 3.3 and 3.4. Tips: To prevent your specimen slide from making contact with an objective, turn the 100X objective in position and adjust the focusing rack stop screw (Fig. 5) so that the 100X objective will not contact the specimen while the stage is adjusted to its highest position. Give the stage a tiny extra moving space to ensure the objective can be focused every time. 3.6 Applying the immersion oil 1) Rotate the objective nosepiece to seat the observing position between the 40X and 100X objectives as shown in Fig. 6 (a). 2) Place a drop of immersion oil on the slide cover as shown in Fig. 6 (b). 3) Rotate the objective nosepiece to seat the 100X objective to the observing position until you hear a click sound. 4) After observing the specimen, use the camera cleaning kit to clean the 100X objective lens gently and the specimen in time. 5) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens and the specimen.
Fig. 5

Fig. 6 (a) (b) Caution (important): When you use the 100X objective to observe the specimen, you have to finish observing the specimen with the 4X, 10X, 40X objectives. When you use the 100X objective to observe the specimen, you have to apply the immersion oil on the top of the slide cover. When you apply the immersion oil with the 100X objective, do not let the immersion oil to contact with the dry objective lenses (especially the 40x). If the immersion oil is on the dry objectives lens, please use the camera cleaning kit to
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www.microscopenet.com clean the objectives lens in time. The oil will damage the dry objective lenses. After observing the specimen with the 100X objective, clean the 100X objective lens first.

3.7 Adjusting condenser 1) Turn the condenser focus knob to raise or lower the condenser. 2) Raise the condenser when using high power objectives and lower it when using low power objectives. Note: The centering of the condenser and the light axis of the objective are factory adjusted. Do not attempt re-adjustment. The highest position of the condenser has been factory adjusted. Do not attempt re-adjustment. 3.8 Adjusting iris aperture diaphragm Swing the iris diaphragm lever (Fig. 7) left or right to adjust the aperture size. Note: The iris diaphragm is designed to adjust the aperture size, not to adjust the brightness although the Iris Diaphragm Lever brightness will be changed when it's adjusted. When aperture is adjusted to smaller size, the contrast will be Fig. 7 increased and the depth of field will be increased as well. Turn up the intensity of the light if the image is too dim. 3.9 Camera (optional, may not included in your package) 1) Bring the microscope into focus by following the procedures in 3.5. 2) Install the camera by following the procedures in 2.4. 3) Open image observing sofeware to examine. 4) You also can capture images or record live videos through the software, depending on the functions provided by the software. Note: Please refer to the manuals in the cameras CD for the details of installation and operation of the camera. 3.10 Dry Darkfield condensers observation (optional, may not included in your
package)

See section 7 (Dry Darkfield Condenser Installation & Operation Instruction)

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4 Specifications
General
Model Total Magnification Viewing Head Interpupillary Distance Diopter Adjustment M82ES series 40X, 80X, 100X, 200X, 400X, 800X, 1000X, 2000X Binocular, inclined 45, swiveling 360 Sliding adjustment, 2-3/16 ~ 2-15/16 (55mm ~ 75mm) On both eyepiece tubes 1 pair of wild field WF10X/18 1 pair of wild field WF20X Revolving quadruple Achromatic DIN 4X/0.10 160/0.17 10X/0.25 160/0.17 40X/0.65 160/0.17(spring), 100X/1.25 160/0.17(spring, oil) Abbe, NA=1.25, w/ iris diaphragm and diffusion filter Rack and pinion adjustment Coaxial coarse and fine focusing knobs on both sides with rack stop Double layer, dimension: 4-1/2"x 4-15/16" (115mm x 125mm) X-Y translational range: 2-13/16 x 1-3/16 (70 mm x 30 mm) Transmitted, LED, variable intensity Refer to the cameras specifications Refer to the Dry Darkfield Condensers Installation & Operation Instruction AC/DC adapter, 7.5V/7.5W (UL approved) - Input: 100-240VAC, 50/60Hz, 0.5A Max - Output: DC7.5V, 1000mA 7-1/8 x 9-1/16 x 13 (18cm x 23cm x 33cm) 7 lbs 2 oz (3.25 kg)

Eyepieces Nosepiece

Objectives

Condenser Focus Mechanism Mechanical Stage Illumination Camera (optional) Dry Darkfield Condensers (optional)

Power Supply Dimension Net weight

Eyepieces
Designation Wide Field Magnification 10X Field of View 18mm Mount Size 23.2mm

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Wide Field 20X 10mm 23.2mm

Magnifications
Eyepiece Objective Magnification 40X 10X 4X 80X 100X 20X 10X 10X 200X 400X 20X 10X 40X 800X 1000X 20X 10X 100X 2000X 20X

5 Optional Parts
(The optional parts may be included in some models or sold separately.) 1) Darkfield Condensers
Model A191 Darkfield Condenser Dry Numerical Aperture 0.7-0.9 Objective Mounting Size(diameter) 37mm

2) Cameras
Model Resolution Operating System MS Windows (32/64-bit) Mac OS Software

A1502

640 x 480 (0.3MP)

A1510

1280 x 1024 (1.3MP)

MS Windows (32/64-bit) MS Windows (32/64-bit) Mac OS

Included

A1520C

1600 x 1200 (2.0MP)

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6 Troubleshooting Guide
Problem
Lamp does not light when switched on

Cause
No electrical power LED or power unit dead Revolving nosepiece not in click stop position

Solution
Check power cord connection Contact seller for service Revolve the nosepiece to click-stop position by swinging the objective correctly into the optical path Check the diffusion filter and make sure its in the light path Swing out the diffusion filter when using 100X objective

Darkness at the periphery

Uneven brightness in the field of view Insufficient brightness

The diffusion filter is not in the light path Diffusion filter used when using 100X objective Dirt or dust on the lens eyepiece, condenser, objective, collector lens or specimen Dirt or dust on the specimen No slide cover attached to the slide Slide cover is too thick or thin Slide may be upside down (specimen at the bottom) Diopter adjustment is not set properly Immersion oil is on a dry objective (especially the 40x)

Clean the lens with a camera cleaning kit

Dirt or dust on the view

Clean the specimen Attach a 0.17mm slide cover Use a slide cover of the appropriate thickness (0.17mm) Turn slide over so the cover-glass faces up Readjust the diopter settings Check the objectives, clean if necessary Use immersion oil Remove bubbles Secure the specimen in the slide holder Open or close properly

Poor image quality or not able to get focused image

No immersion oil used with 100x Air bubbles in immersion oil Specimen rises from stage surface Condenser aperture is closed or open too much Condenser is positioned too low or too high Lamp intensity is too high or low

Move condenser upper or lower Adjust the light intensity by rotating the intensity control dial Check the camera manual

Image is black screen through camera (optional)

No enough lighting or driver not installed properly

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Image is totally white through camera (optional) The light is too bright Manually adjust the exposure in the software. See manual for details.

7 Dry Darkfield Condenser Installation & Operation Instruction

1) Mounting the dry darkfield condenser Condenser Holder Rotate the nosepiece and set the 4x objective in Condenser Lock position. Thumb Screw Loosen and raise the focusing rack stop screw to the highest position. Turn the coarse focus knob to raise the mechanical stage to the highest position without contacting the 4X objective. Centering Screws Turn the condenser control knob to raise the condenser to the highest position. Fig. 8 Loosen the condenser lock thumb screw, pull down the condenser and take it off, insert the dry darkfield condenser in, and tighten the condenser lock thumb screw (See Fig. 8). Re-adjust the condenser height to its normal position. Re-adjust the mechanical stage height to its normal position. Lower the focusing rack stop screw back and use the bolt to lock it when needed. Note: When raising the mechanical stage, do not make contact with the objective. 2) Centering the dry darkfield condenser Turn the 4X objective to the light path. Turn the condenser focus knob to lower the condenser till a dark spot showed in the viewing field as shown in Fig. 9 (a). Turn the condenser translational centering screws to move the dark spot to the center (a) (b) Fig. 9 as shown in Fig. 9 (b). 3) Place the slide on the stage. 4) Raise the condenser all the way to the top and then lower it a little bit. 5) Follow the procedures in this manual to bring the sample on slide in focus and observe. 6) Move the condenser up or down slightly to get the best darkfield viewing. Note The dry darkfield condenser is used with the dry objectives only. The dry darkfield condenser works with the 4X, 10X, 40X objectives. The dry darkfield condenser does not work with the 100X oil immersion objective.

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