LQ181V

Mannitol Salt Broth LQ181V
Intended Use
Recommended for selective isolation of presumptive pathogenic Staphylococci.
Composition**
Ingredients Gms / Litre
Proteose peptone 10.000
HM peptone B # 1.000
Sodium chloride 75.000
D-Mannitol 10.000
Phenol red 0.025
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Label the ready to use LQ181V bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation

Mannitol Salt Broth is prepared as suggested by Chapman (2) and is used for the selective isolation of pathogenic
Staphylococci. This medium is recommended for the detection and enumeration of coagulase-positive Staphylococci in milk
(6) food (1) and other specimens. Mannitol Salt Broth is used for the isolation of presumptive pathogenic staphylococci.
Pathogenic staphylococci ferment mannitol and produce a yellow coloured medium.
The medium contains HM peptone B and proteose peptone which makes it very nutritious as they provide essential growth
factors and trace nutrients. Many other bacteria except Staphylococci are inhibited by 7.5% sodium chloride. Mannitol is the
fermentable carbohydrate source. The differential action of the medium is attributed to D-Mannitol. Staphylococcus aureus
ferments mannitol to produce yellow coloured medium. Most coagulase-negative species of Staphylococci and Micrococci do
not ferment mannitol and therefore the medium remains red in colour. The colour of the medium is due to the reactivity of
phenol red to the pH of the medium; phenol red is red at pH 8.4 and yellow at 6.8. Presumptive Staphylococcus showing
yellow coloured medium should be further tested for production of coagulase.
A possible S. aureus must be confirmed by the coagulase test. Also the organism should be subcultured to a less inhibitory
medium not containing excess salt to avoid the possible interference of salt with coagulase testing or other diagnostic tests
(e.g. Nutrient Broth) (M002) (5). Few strains of S. aureus may exhibit delayed mannitol fermentation. Negative results
should therefore be re-incubated for an additional 24 hours before being discarded (5).
Type of specimen
Clinical samples: nasal and skin lesions & swabs, abscess, wound exudates, pus or discharge; Food & Dairy samples
Specimen Collection and Handling

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
For food and dairy samples follow appropriate techniques for handling specimens as per established guidelines (6,7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye
protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may
be referred in individual safety data sheets.
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
Limitations
1. A possible S.aureus must be confirmed by the coagulase test.
2. The organism should be subcultured to a less inhibitory medium not containing excess salt to avoid the possible
interference of salt with coagulase testing or other diagnostic tests (e.g. Nutrient Broth) (M002) (5).
3. Few strains of S.aureus may exhibit delayed mannitol fermentation. Negative results should therefore be re-incubated for
an additional 24 hours before being discarded (5).
Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
at recommended temperature.
Quality Control
Appearance
Sterile Mannitol Salt Broth in glass bottle
Colour
Red coloured clear solution
Quantity of Medium
5 ml of medium in glass bottle
pH
7.20-7.60
Sterility Testing
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
Organism Inoculum Growth Colour of
(CFU) medium
4
Escherichia coli ATCC >=10 Inhibited
25922 (00013*)
Staphylococcus aureus 50-100 good-luxuriant yellow
subsp. aureus ATCC
25923 (00034*)
Staphylococcus epidermidis 50-100 fair-good red
ATCC 12228 (00036*)
Key : (*) Corresponding WDCM numbers.
Storage and Shelf Life

On receipt store between 15-25°C. Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
Reference
1. Bacteriological Analytical Manual, 1995, Food and Drug Administration, 8th ed., AOAC, International, U.S.A.
2. Chapman G.H., 1945, J. Bact., 50:201.
3. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
4. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
5. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams
and Wilkins, Baltimore
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
6. Marshall R. (Ed.), 1992, Standard Methods for the Examination of Dairy Products, 16th ed., APHA, Washington, D.C.
7. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th
Ed., American Public Health Association, Washington, D.C.
Revision : 00 / 2019
In vitro diagnostic medical

IVD device
CE Marking
25°C Storage temperature
15°C
Do not use if package is

damaged
HiMedia Laboratories Pvt. Limited,

23 Vadhani Industrial Estate,
LBS Marg,Mumbai-86,MS,India
CE Partner 4U ,Esdoornlaan 13, 3951

EC REP
DB Maarn The Netherlands,
www.cepartner 4u.eu
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office :
A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
[email protected] Website: www.himedialabs.com

LQ181V

Uploaded by

Copyright:

Available Formats

LQ181V

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

LQ181V

Uploaded by

Copyright:

Available Formats

Mannitol Salt Broth LQ181V

Principle And Interpretation

Specimen Collection and Handling

Please refer disclaimer Overleaf.

Performance and Evaluation

Storage and Shelf Life

Please refer disclaimer Overleaf.

In vitro diagnostic medical

25°C Storage temperature

Do not use if package is

HiMedia Laboratories Pvt. Limited,

CE Partner 4U ,Esdoornlaan 13, 3951

You might also like