Peptone Water M028

Intended Use:
Peptone Water is used as a growth medium and as a base for carbohydrate fermentation media.
Composition**
Ingredients Gms / Litre
Peptone 10.000
Sodium chloride 5.000
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 15.0 grams in 1000 ml distilled water. Add the test carbohydrate in desired quantity and dissolve completely.
Dispense in tubes with or without inverted Durhams tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15
minutes.
Principle And Interpretation
Peptone Water is particularly suitable as a substrate in the study of indole production. Peptone used in Peptone Water is
rich in tryptophan content. Presence of indole can be demonstrated using either Kovacs or Ehlrich reagent. Peptone Water
is also utilized as a base for carbohydrate fermentation studies with the addition of sugar and indicators such as
bromocresol purple, phenol red or bromothymol blue.
Peptone Water is recommended (3,6,7) for studying the ability of an organism to ferment a specific carbohydrate which
aid in differentiation of genera and species. Peptone water is formulated as per Shread, Donovan and Lee (9). Peptone
Water with pH adjusted to 8.4 is suitable for the cultivation and enrichment of Vibrio species. Peptone
provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins provides essential nutrients.
Sodium chloride maintains the osmotic balance of the medium. To study the fermentation ability of
carbohydrates, saccharose, rhamnose, salicin are generally added in 0.5% amount separately to the basal medium before or
after sterilization. The acidity formed during fermentation can be detected by addition of phenol red indicator, which
shows a colour change of the medium from red to yellow under acidic conditions. If desired, Durham's tube may be used
to detect the gas production if produced.

Type of specimen
Isolated microrganism from clinical specimen , food, dairy and water samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,8,10).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(2)
After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye
protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets.

Limitations
1. Due to nutritional variations , some strains may show poor growth.

Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
at recommended temperature.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured clear solution without any precipitate
Reaction
Reaction of 1.5% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
Organism Inoculum Growth Indole test
(CFU)

Staphylococcus aureus 50-100 luxuriant negative reaction, no red ring at

subsp. aureus ATCC the interface of the medium on
25923 (00034*) addition of Kovac's reagent (R008)

Escherichia coli ATCC 50-100 luxuriant positive reaction, red ring at the
25922 (00013*) interface of the medium on
addition of Kovac's reagent (R008)
Salmonella Typhimurium 50-100 luxuriant negative reaction, no red ring at the
ATCC 14028 (00031*) interface of the medium on
addition of Kovac's reagent (R008)

Key : *Corresponding WDCM numbers.

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the
label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation
due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry
ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before
expiry date on the label. Product performance is best if used within stated expiry period.

Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).

References
1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington
D.C.
2. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and
Wastewater, 23rd ed., APHA, Washington, D.C.
3. Finegold and Baron, 1986, Bailey and Scotts Diagnostic Microbiology, 7th ed., The C.V. Mosby Co., St. Louis.
nd
4. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2 Edition.
5. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
6. Lennette and others (Eds.), 1985, Manual of Clinical Microbiology, 4th ed, ASM, Washington, D.C.
7. MacFaddin J., 1980, Biochemical Tests for Identification of Medical Bacteria, 2nd ed., Williams and Wilkins, Baltimore.

Please refer disclaimer Overleaf.

 HiMedia Laboratories Technical Data

8. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th
Ed., American Public Health Association, Washington, D.C.
9. Shread P., Donovan T.J, and Lee J.V, (1981), Soc. Gen, Microbiol. Q., 8, 184.
10. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,
APHA Inc., Washington, D.C.

Revision : 04 / 2019

In vitro diagnostic medical

IVD device

CE Marking

30°C Storage temperature

10°C

Do not use if package is

damaged

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Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

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