Q.P. Code: 262951

THE TAMIL NADU DR. M.G.R.
MEDICAL UNIVERSITY
[LL 951] NOVEMBER 2017 Sub. Code: 2951
M.PHARM. DEGREE EXAMINATION

(PCI New regulations 2016)
SEMESTER-I
PHARMACEUTICAL ANALYSIS  MPA
PAPER I – MODERN PHARMACEUTICAL ANALYTICAL
TECHNIQUES
Q.P. Code : 262951
Time : Three hours Maximum : 75 Marks
I. Elaborate on: (2 x 20 = 40)
1. a) Explain the theory and principle of Nuclear magnetic resonance.

b) Explain the terms equivalence of protons, chemical shift and coupling.
c) Draw the NMR spectrum expected for the compound 1, 3-dichloropropane.
Indicate the equivalent protons, number of signals expected, relative positions
of the signals and the splitting pattern of the signals.
2. a) Explain the principle of Mass Spectrometry.

b) Describe the working of a Mass Spectrometer.
c) Explain the McLafferty rearrangement.
II. Write notes on: (7 x 5 = 35)
1. Heat Flux differential scanning calorimetry.

2. Rotating crystal method of studying crystal structures.
3. Factors affecting fluoresence.
4. Affinity chromatography.
5. Derivatisation techniques used in chromatography.
6. Ion selective glass electrodes.
7. Explain the finger print region and functional group regions in an IR spectrum.
*********
THE TAMIL NADU DR. M.G.R. MEDICAL UNIVERSITY
[LM 951] MAY 2018 Sub. Code: 2951

SEMESTER-I
TECHNIQUES
Q.P. Code : 262951
1. a) Discuss the different columns and detectors used in Gas chromatography.

b) Explain the terms Retention time and Retention volume.
c) Explain Head Space Analysis.
2. a) Explain the different methods of ionization employed in Mass Spectrometry.

b) Discuss the different methods of fragmentation.
1. Different types of quenching.

2. Derivative spectroscopy.
3. Gel electrophoresis.
4. Differential Thermal analysis.
5. Draw the NMR spectrum expected for the compound Ethyl bromide, indicating
the number of signals, their positions and the splitting of the signals. Justify the
relative positions of the signals with respect to TMS.
6. Calculate the vibrational degrees of freedom for a linear molecule like carbon
dioxide.
7. Explain the terms chemical shift and coupling.
*********
[LN 951] NOVEMBER 2018 Sub. Code: 2951

SEMESTER-I
TECHNIQUES
Q.P. Code : 262951
1. a) What are the factors that influence vibrational frequencies? Explain.

b) What are the different modes of molecular vibrations? Discuss.
c) How many fundamental bands will be observed in the IR spectrum of
benzene?
2. a) Explain the theory and principles of NMR spectroscopy.

b) What is meant by relaxation? Explain.
c) For the compound Butanone -2, what will be the number of signals, splitting of
signals and position of the signals relative to TMS? Draw the spectrum.
1. Factors affecting Thermogravimetric analysis.

2. X-ray powder diffraction technique.
3. Matrix Assisted Laser Desorption Ionisation (MALDI).
4. Height equivalent of a theoretical plate.
5. Ion exchange chromatography.
6. Homolytic cleavage in Mass Spectrometry.
7. Woodward fisher rule.
*******
[LO 951] MAY 2019 Sub. Code: 2951

SEMESTER-I
TECHNIQUES
Q.P. Code : 262951
1. a) Write in detail on various principles of separation and chromatographic

parameters of HPLC.
b) Calculate the Hydrogen deficiency for the compound C6H5COOH.
c) Discuss the 1HNMR signals of = Toluene.
2. a) Explain the types of ionization in Mass spectrometry. Draw and label single
quadrupole mass instrument.
b) Write on peaks and application of Mass spectrometry.
1. Write differential scanning calorimetric interpretation of two compounds.

2. Concepts of capillary electrophoresis.
3. With neat diagram give working of Katherometer and Electron capture detector.
4. Factors affecting vibration frequencies of IR spectra.
5. Write the principles of fluorimetry.
6. Write about elemental analysis by Flame emission spectroscopy.
7. Construction and working of a double beam spectrophotometer.
******
[LP 951] NOVEMBER 2019 Sub. Code: 2951

SEMESTER-I
BRANCH III – PHARMACEUTICAL ANALYSIS  MPA
TECHNIQUES
Q.P. Code : 262951
1. Write on the principle, relaxation process, chemical shift and its factors affecting,
coupling constant and applications of NMR spectroscopy.
2. a) Elaborate on principles, instrumentation and deletion used in Gas
chromatography.
b) Give the IR Interpretation for:
i) C6H5CHO ii) 2 – Hexene.
1. Principles of analysis of drugs by UV absorption.

2. Biomolecular applications of gel chromatography.
3. Theory and types of fluorescence quenching.
4. Atomic absorption spectroscopy-principles and interferences.
5. Write on electrophoresis types.
6. Write about X-ray powder diffraction in structural analysis.
7. Describe the principles of TGA, DTA and DSC.
*******
[LQ 0121] JANUARY 2021 Sub. Code: 2951

(APRIL 2020 EXAM SESSION)
M.PHARMACY DEGREE EXAMINATION
SEMESTER-I (PCI New regulations 2016)
PAPER I – MODERN PHARMACEUTICAL ANALYSIS
Q.P. Code : 262951
Time : Three hours Answer ALL Questions Maximum : 75 Marks
1. a) Write the principle, diffraction methods and applications of X-ray diffraction

analysis.
b) Write a note on moving boundary electrophoresis.
2. a) Explain the principle and instrumentation of AAS.
b) Indicate what NMR spectra you expect from the following compounds.
a) CH3-CH2-CH2-CH3
CH3
b) CH3-C-CH3
CH3
c) Write the principle and instrumentation on Flame photometry.
1. Explain about the relaxation process in NMR spectroscopy.

2. Write about the columns used in HPLC.
3. Write about the ions produced in Mass Spectroscopy.
4. Write a note on NMDR.
5. Write the ion collectors used in Mass Spectroscopy. Explain with a neat labeled diagram.
6. Isotopic peaks in Mass Spectroscopy.
7. Write the detectors used in HPLC. Explain about UV-Visible detector.
*******
[MPHARM 0921] SEPTEMBER 2021 Sub. Code: 2951

(OCTOBER 2020 EXAM SESSION)

PHARMACEUTICAL ANALYSIS - MPA
PAPER I – MODERN PHARMACEUTICAL ANALYTICAL TECHNIQUES
Q.P. Code : 262951
1. a) Write the instrumentation and solvent requirement in NMR spectroscopy.

b) Write a detailed note on 13C NMR.
c) Predict the number of peaks with relative intensity in the spectra of Toluene.
2. a) Write the principle and instrumentation involved in HPLC.

b) Discuss in detail about the developmental techniques and detection techniques
in TLC.
1. Explain the thermal transitions in DTA curve.

2. Write about the electronic transitions in UV spectroscopy.
3. Explain the sampling techniques in IR Spectroscopy.
4. Explain the theory of fluorescence with Jablonsky diagram.
5. Write a note on types of ion exchangers and the mechanism involved in ion
exchange chromatography.
6. Write notes on any two mass analyzers.
7. Explain capillary electrophoresis.
*******
[MPHARM 0122] JANUARY 2022 Sub. Code: 2951


Q.P. Code : 262951
1. a) Explain different types of ionization techniques in Mass Spectrometry. Draw

and label a single quadrupole mass instrument.
b) Discuss the Woodward Fieser rule and its application.
2. a) Discuss the theory of fluorescence and factors affecting fluorescence.
b) Write the instrumentation and applications of fluorimetry.
1. Calculate the vibrational modes for linear molecule ethaneC2H6 and nonlinear
molecule C6H6.
2. Differentiate dispersive I.R from F.T. IR.
3. Write short notes on Spin-Spin coupling.
4. Derive Beer lamberts law.
5. The molar absorption co-efficient of tyrosine in water is 1280 M-1 cm-1 at 280 nm.
Calculate the concentration of tyrosine solution in water if the absorbance of the
solution is 0.34 measured in a 1 cm path length cell.
6. Enumerate the detectors used in Gas chromatography. Explain any one in detail.
7. Write a note on Bragg’s law.
*******
[MPHARM 0422] APRIL 2022 Sub. Code: 2951


Q.P. Code : 262951
1. a) Explain the different modes of molecular vibrations in I.R .Spectroscopy.

b) Discuss the factors affecting vibrational frequencies in I.R.
c) Explain sample handling Techniques in IR Spectroscopy.
2. a) Draw a neat labeled diagram of Double beam UV spectrophotometer and

explain construction and working.
b) Calculate the concentration of compound X using A (1%, 1cm) 9 at wavelength
257nm in a 1.5cm cell with absorbance 0.4527.
1. Explain the different types of interferences in Flame photometry.

2. Write about capillary electrophoresis.
3. Write a short note on Coupling constants.
4. Enumerate the different ionization techniques used in mass spectrometry. Explain
MALDI.
5. Explain the applications of HPLC.
6. Iso-electric focusing.
7. Predict the number of NMR peaks with relative intensity in the spectra of
Acetaldehyde.
*******
[M.PHARM 0922] SEPTEMBER 2022 Sub. Code: 2951


SEMESTER - I (PCI New regulations 2016)
Q.P. Code : 262951
1. (a) Describe the principle and working of HPLC with a neat labeled diagram.
(b) Calculate the concentration of compound X using A (1%, 1cm) 9 at wave
length 257nm, at a 1.5cm Cell, with absorbance 0.4527.
2. (a) With neat labeled diagram, explain the principle and instrumentation of
Double beam UV Spectrophotometer.
(b) Write a note on 13C NMR and spin-spin coupling.
1. Write about the McLafferty rearrangement.

2. Write a note on any two mass analyzers.
3. Describe Bragg’s law and applications of x-Ray diffraction.
4. Explain the sampling techniques in IR spectroscopy.
5. Give an IR interpretation for
(i) C6H5CHO (ii) 2-butanone.
6. Discuss the factors affecting fluorescence.
7. Write a note on Electrophoresis techniques.
*******
[M.PHARM 0423] APRIL 2023 Sub. Code: 2951


SEMESTER - I (PCI New regulations 2016)
Q.P. Code: 262951
1. a) Explain in detail about the fragmentation rules in electron impact mass

Spectrometry. Give suitable examples.
b) Explain different analysers used in mass spectroscopy.
2. a) Explain the principle and instrumentation of a spectrofluorimeter with a neat
diagram.
b) Discuss the Woodward Feiser rule and its applications.
1. Give the construction and working principle of Time of Flight Mass analyser.
2. Define the following with equations:
a) HETP b) Capacity factor c) Retention time.
3. Write the principle and working of flame emission spectrophotometer.
4. Differentiate dispersive IR and FT IR.
5. Discuss the factors affecting vibrational frequencies in IR.
6. Explain the applications of HPLC.
7. Explain the applications of NOESY and COSY.
*******
[M.PHARM 0823] AUGUST 2023 Sub. Code: 2951


SEMESTER - I (PCI New Regulations 2016)
Q.P. Code: 262951
1. a) What are the basic requirements for absorption of IR radiation? Explain with
suitable examples.
b) Write an account on chromophores with suitable examples. Discuss the choice
of Solvents and solvent effects in absorption.
2. (a) What do you mean by nuclear Overhauser effect (NOE)? What are its
applications?
(b) Explain the chemical shift data of the following compounds:
1. Benzene-C6H6 δ =7.37 2. Acetylene –C2H2 δ=2.9
3. Toluene C6H5CH3 δ=2.32 4. Cyclohexane C6H12 δ=5.57
5. Aniline C6H5NH2 δ=6.44.
1. Discuss spin-spin coupling and factors affecting spin-spin coupling.

2. Write about ion exchange chromatography.
3. Define the following terms:
a) Accuracy b) Precision c) LOD d) LOQ.
4. Explain about factors affecting fluorescence intensity.
5. Write about affinity chromatography and its applications.
6. Write construction and working of Electron Capture Detector.
7. Calculate the concentration of methyltestosterone in an ethanolic solution of which
the absorbance in a 1 cm cell at its ʎ max, 241nm, was found to be 0.890.
The A (1%, 1cm) in the B.P.monograph of methyltestosterone is given as 540 at
241nm.
*******
[M.PHARM 1223] DECEMBER 2023 Sub. Code: 2951


SEMESTER - I (PCI New Regulations 2016)
Q.P. Code: 262951
Time: Three hours Answer ALL Questions Maximum: 75 Marks
1. a) Explain the principle, instrumentation, working and application of UV-Visible

spectrophotometry.
b) Write about types electronic transitions in UV – spectroscopy.
2. a) Explain the theory of mass spectrometry.

b) Write the different types of ionisation technique in mass spectrometry.
c) Write about the time of flight mass analyzer.
1. Write the different types of molecular vibration by absorption of IR radiation by

molecules.
2. Write the interferences of flame photometry. How will you overcome that?
3. Write a note on spin – spin coupling.
4. Predict the structural formula for the compounds with the following molecular
formulae showing only one PMR signal each.
a) C8H18 b) C2H6O c) C5H12
5. How will you determine the crystal structure by Bragg’s spectrometric method?
6. Explain factors affecting the intensity of fluorescence.
7. Write a note on Iso electric focusing electrophoresis.
*******

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THE TAMIL NADU DR. M.G.R.

[LL 951] NOVEMBER 2017 Sub. Code: 2951

M.PHARM. DEGREE EXAMINATION

Q.P. Code : 262951

Time : Three hours Maximum : 75 Marks

I. Elaborate on: (2 x 20 = 40)

1. a) Explain the theory and principle of Nuclear magnetic resonance.

2. a) Explain the principle of Mass Spectrometry.

II. Write notes on: (7 x 5 = 35)

1. Heat Flux differential scanning calorimetry.

[LM 951] MAY 2018 Sub. Code: 2951

M.PHARM. DEGREE EXAMINATION

Q.P. Code : 262951

Time : Three hours Maximum : 75 Marks

I. Elaborate on: (2 x 20 = 40)

1. a) Discuss the different columns and detectors used in Gas chromatography.

2. a) Explain the different methods of ionization employed in Mass Spectrometry.

II. Write notes on: (7 x 5 = 35)

1. Different types of quenching.

[LN 951] NOVEMBER 2018 Sub. Code: 2951

M.PHARM. DEGREE EXAMINATION

Q.P. Code : 262951

Time : Three hours Maximum : 75 Marks

I. Elaborate on: (2 x 20 = 40)

1. a) What are the factors that influence vibrational frequencies? Explain.

2. a) Explain the theory and principles of NMR spectroscopy.

II. Write notes on: (7 x 5 = 35)

1. Factors affecting Thermogravimetric analysis.

[LO 951] MAY 2019 Sub. Code: 2951

M.PHARM. DEGREE EXAMINATION

Q.P. Code : 262951

Time : Three hours Maximum : 75 Marks

I. Elaborate on: (2 x 20 = 40)

1. a) Write in detail on various principles of separation and chromatographic

II. Write notes on: (7 x 5 = 35)

1. Write differential scanning calorimetric interpretation of two compounds.

[LP 951] NOVEMBER 2019 Sub. Code: 2951

M.PHARM. DEGREE EXAMINATION

Q.P. Code : 262951

Time : Three hours Maximum : 75 Marks

I. Elaborate on: (2 x 20 = 40)

II. Write notes on: (7 x 5 = 35)

1. Principles of analysis of drugs by UV absorption.

[LQ 0121] JANUARY 2021 Sub. Code: 2951

Time : Three hours Answer ALL Questions Maximum : 75 Marks

I. Elaborate on: (2 x 20 = 40)

1. a) Write the principle, diffraction methods and applications of X-ray diffraction

II. Write notes on: (7 x 5 = 35)

1. Explain about the relaxation process in NMR spectroscopy.

[MPHARM 0921] SEPTEMBER 2021 Sub. Code: 2951

M.PHARMACY DEGREE EXAMINATION

Time : Three hours Answer ALL Questions Maximum : 75 Marks

I. Elaborate on: (2 x 20 = 40)

1. a) Write the instrumentation and solvent requirement in NMR spectroscopy.

2. a) Write the principle and instrumentation involved in HPLC.

II. Write notes on: (7 x 5 = 35)

1. Explain the thermal transitions in DTA curve.

[MPHARM 0122] JANUARY 2022 Sub. Code: 2951

M.PHARMACY DEGREE EXAMINATION