KILOBASER EMPOWERS

EVERYONE TO
CREATE DNA & RNA
Speed up your research with Kilobaser
DNA & RNA synthesizers and experience
efficiency in perfection. Synthesizing DNA
and RNA oligos has never been easier. Get
your ready-to-use custom oligos in less
than 2 hours.

THE FASTEST WAY

TO DNA & RNA
GREAT PRODUCTS
FOR GREAT MINDS

Index

Synthesizer 04 - 09 Customized Cartridge 26 - 27

Our Technology 04 - 05 Microfluidic Chips 28 - 39

Specifications 06 - 07 Standard Chip 28 - 29

Workflow 08 - 09 6-FAM Label Chip 30 - 31

DNA Oligos 10 - 11 BHQ-1 Chip 32 - 33

6-FAM DNA Probes 12 - 13 Amino Modifier C6 Label Chip 34 - 35

5’-Modifications 14 - 19 Biotin Label Chip 36 - 37

Amino Modifier C6 14 - 15 Phosphorylation Chip 38 - 39

Biotin 16 - 17 Supply 40 - 41

Phosphorylation 18 - 19 OliPure HIC Purification Kit 40 - 41

Fully Modified RNA Oligos 20 - 25

2’-MOE 20 - 21

2’-OMe 22 - 23

2’-F 24 - 25
KILOBASER
SYNTHESIZER

Our Technology

Create essential molecular tools in record time on your own

lab bench using Kilobaser DNA & RNA synthesizers.

Kilobaser one & one-XT (27x33x33cm) are the most com-

pact of their kind. Not only do our devices operate in a stand-
alone manner, but they can also be integrated seamlessly
into a fully automated work process.

Kilobaser’s revolutionary technology allows for independent

and rapid DNA & RNA synthesis without knowledge of oligo-
nucleotide synthesis chemistry.

Our key innovation is the combined use of a cartridge with

a microfluidic chip. The cartridge contains all the reagents
required for the synthesis, while the chip carries the reaction
column in which the synthesis takes place.

Microfluidic chips carry the

micro-column and form the
base of the synthesis system.

Each cartridge provides all

reagents for multiple syntheses, Get all specifications here:
depending on strand length. http://oligo.link/specifications

Synthesizer
4 5
KILOBASER one
& KILOBASER one-XT

Specifications

Kilobaser Kilobaser
one one-XT

Automated Synthesis

DNA Oligo

DNA Probe

Modified DNA

Modified RNA

Basic Support*

Extended Support*

Scientific Support*

Kilobaser one
*Basic Support: As soon as the Kilobaser arrives at your site, we assist EUR 30.000 / USD 35,500
you through connection and installation in the course of an online set-up
call. We are happy to support you in your initial DNA synthesis to prepare Kilobaser one-XT
the Kilobaser for your custom oligo syntheses. EUR 42.000 / USD 49,500

*Extended Support: We provide a detailed introduction to all the pos-

sible modifications and explain how to create your desired modified Which device should you choose?
oligonucleotides using the Kilobaser cartridge-and-chip system. This
includes a step-by-step introduction to our OliPure HIC Purification Kit Kilobaser one offers the best price-performance ratio for just DNA oligo
and manual cleaning in case the cartridge type is changed. synthesis and is thus ideal in case you mainly need primers or other un-
modified DNA oligonucleotides.
*Scientific Support: We are happy to help you evaluate your syntheses
and experiments with oligonucleotides synthesized with the Kilobaser Kilobaser one-XT - the ultimate synthesis tool that works seamlessly
and troubleshoot together, discussing further steps. We also offer to with all our existing cartridges and chips! Whether you need to synthesize
analyze your synthesized oligonucleotides in our laboratory to assure primers, probes or other modified oligos, the Kilobaser one-XT has you
their quality. fully supported.

6 7
READY-TO-USE OLIGOS
SIMPLE AND FAST

Workflow

Science is hard enough already,

let us make it a little easier for you!

Enter sequence
1
Type in or transfer the
sequence via USB or PC

Insert chip
2
Error-free handling due
to user-centered design

Press start
3
Get your ready-to-use
DNA/RNA within two hours

The product is automatically dried

in a PCR type vial for storage and
further processing.

8
STANDARD DNA
REAGENT CARTRIDGE DMTO Base Ndef NHAc O
H3C
O

O N N
N N NH NH

N N N N N N
O Ndmf O

Phosphoramidite Adenosine Cytidine Guanosine Thymidine

Technical Specifications
Phosphoramidite for building DNA oligos

Volume 150nt Chemistry

To synthesize the DNA strand, phoshoramidites with the respective
Nucleotides Deoxyribonucleotides bases are used as building blocks. These blocks contain a base, a
DMT group, and a phosphoramidite bound to a ribose sugar ring.
Bases Quantity 75 x A, C, G, T
How does it work?
5’-Modification No DNA synthesis is performed from 3’ to 5’ by coupling one phospho-
ramidite after another to the microcolumn. For each coupling step,
Usage DNA oligo synthesis first the DMT group is cleaved, providing a free 5’-hydroxy group to
react with the 3’-phosphoramidite. The resulting phosphite is then
oxidized to phosphate. In the final step of the synthesis, the oligonu-
Shelf Life 1 year
cleotide is cleaved from the column and the protective groups are
removed to yield functional DNA.
Shelf Life after Activation 2 weeks

Short-Term Storage 2 months at room temperature

Long-Term Storage 2-8°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one or Kilobaser one-XT

What’s inside?
Price
The cartridge contains all the
reagents required for chemical EUR 90,00 / USD 110.00
DNA synthesis. This includes DNA Bases
the bases A, C, G and T, which
are positioned in the center of A C G T
the cartridge with colored caps. Chip Combination
The presence of an excess
amount of bases ensures the
availability of all four phosphor-
amidites until the final synthesis.

DNA Oligos
Standard Chip (p.28)

10 11
6-FAM DNA
REAGENT CARTRIDGE 495nm 517nm

Fluorescein

N
H Phosphoramidite
Technical Specifications
Phosphoramidite for building fluorescein labeled oligonucleotides

Volume 100nt Chemistry

The 6-carboxyfluorescein (6-FAM) DNA phosphoramidite contains a
Nucleotides Deoxyribonucleotides 6-FAM on a chain consisting of 6 carbon atoms and is coupled to the
last base of the DNA strand, resulting in a 6-FAM at the 5’-end.
Bases Quantity 75 x A, C, G, T
Advantages
5’-Modification 6-FAM dye 6-FAM has an emission wavelength of 517nm, which a wide variety
of detection devices can measure. Due to the additional C6 linker, the
Usage Un-/single-/dual-labeled fluorophore affects neither the DNA strand nor the duplexes it forms.
DNA synthesis
Potential applications
6-FAM labeled oligos can be widely used as single-labeled probes and
Shelf Life 1 year
as dual-labeled probes with an additional quencher. Find out more about
single-labeled oligos on page 30 and dual-labeled oligos on page 32.
Shelf Life after Activation 1 week

Short-Term Storage 2 months at 2-8°C

Long-Term Storage -20°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one-XT

5’-modification
What’s inside?
Price
The cartridge contains all the rea-
gents required for chemical DNA EUR 90,00 / USD 110.00
synthesis. This includes the bases DNA Bases
A, C, G and T, which are positioned
in the center of the cartridge with A C G T
colored caps. Additionally, 6-FAM is Chip Combination

6-FAM DNA Probes

available for the 5’-modification and
is positioned in the top left corner.

Standard Chip (p.28) BHQ-1 Quencher Chip (p.32) 6-FAM Label Chip (p.30)

12 13
AMINO MODIFIER C6
REAGENT CARTRIDGE HN
Protective group
O
H
N
Phosphoramidite
O
Technical Specifications
Phosphoramidite for building amino-modified oligonucleotides

Volume 100nt Chemistry

The 5’-amino modifier C6 phosphoramidite contains a protected primary
Nucleotides Deoxyribonucleotides amine on a chain of 6 carbon atoms. After coupling to the last base of the
oligonucleotide, all protective groups are cleaved off in the final step of the
Bases Quantity 75 x A, C, G, T synthesis, resulting in a primary amine at the 5’-terminus of the DNA strand.

5’-Modification Amino Modifier C6 Advantages

The main advantage of the amine is its reactivity to numerous functional
Usage Un-/single-labeled DNA synthesis groups. Due to the C6 linker, the interaction of the amine with other groups
affects neither the DNA strand nor the duplexes it forms.
Shelf Life 1 year
Potential applications
Because of the large number of different immobilization reagents available,
Shelf Life after Activation 1 week various surfaces can be functionalized with amino-modified oligos. Find out
more on page 34.
Short-Term Storage 2 months at 2-8°C

Long-Term Storage -20°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one-XT

5’-modification
What’s inside?
Price
The cartridge contains all the reagents
required for chemical DNA synthesis. EUR 90,00 / USD 110.00
This includes the bases A, C, G and T, DNA Bases
which are positioned in the center of the
cartridge with colored caps. Additionally, A C G T
amino modifier C6 is available for the Chip Combination
5’-modification and is positioned in the

5’-Modifications
left corner.

Standard Chip (p.28) Amino Modifier C6 Chip (p.34)

14 15
BIOTIN
REAGENT CARTRIDGE
O

DMTN NH
Protective group

Biotin H
N
S Phosphoramidite

Technical Specifications O

Phosphoramidite for building biotin-labeled oligonucleotides

Volume 100nt Chemistry

The 5’-biotin phosphoramidite contains one biotin group on a chain of 6
Nucleotides Deoxyribonucleotides carbon atoms. The biotin is protected by a DMT group, which is cleaved off
in the final step of the synthesis.
Bases Quantity 75 x A, C, G, T
Advantages
5’-Modification Biotin The advantage of biotin is its strong binding affinity to streptavidin or avidin.
Due to the C6 linker, the binding of biotin affects neither the DNA strand nor
Usage Un-/single-labeled DNA synthesis the duplexes it forms.

Potential applications
Shelf Life 1 year
Biotinylated oligos are ideal to functionalize other surfaces with the features
of DNA oligonucleotides, such as hybridization with a complimentary strand.
Shelf Life after Activation 1 week Find out more on page 36.

Short-Term Storage 2 months at 2-8°C

Long-Term Storage -20°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one-XT

5’-modification
What’s inside?
Price
The cartridge contains all the reagents re-
quired for chemical DNA synthesis. This EUR 90,00 / USD 110.00
includes the bases A, C, G and T, which are DNA Bases
positioned in the center of the cartridge with
colored caps. Additionally, biotin is available A C G T
for the 5’-modification and is positioned in Chip Combination
the top left corner.

Standard Chip (p.28) Biotin Label Chip (p.36)

16 17
PHOSPHORYLATION CN

REAGENT CARTRIDGE Protective group

O NH O
P
O O O N

HN O

Technical Specifications O

Phosphoramidite for building 5‘-phosphorylated oligonucleotides

Volume 100nt Chemistry

The phosphate group for the phosphorylation comes from the phosphor-
Nucleotides Deoxyribonucleotides amidite itself, which consists of the phosphate and protective groups. The
protective groups are cleaved off in the final step of the synthesis, resulting
Bases Quantity 75 x A, C, G, T in a free phosphate group at the 5’-terminus of the DNA strand.

5’-Modification Phosphate Advantages

Using this phosphoramidite directly in the DNA synthesis, the phosphorylation
delivers higher yields than using kinases.
Usage Un-/single-labeled DNA synthesis
Potential applications
Shelf Life 1 year
Phosphorylation converts the synthetic DNA strands to natural ones as
they miss a phosphate at the 5’-end. This phosphate is essential for several
Shelf Life after Activation 1 week innovative applications. Find out more on page 38.

Short-Term Storage 2 months at 2-8°C

Long-Term Storage -20°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one-XT

5’-modification
What’s inside?
Price
The cartridge contains all the reagents re-
quired for chemical DNA synthesis. This EUR 90,00 / USD 110.00
includes the bases A, C, G and T, which are DNA Bases
positioned in the center of the cartridge with
colored caps. Additionally, a phosphorylation A C G T
reagent is available for the 5’-modification Chip Combination
and is positioned in the top left corner.

Standard Chip (p.28) Phosphorylation Chip (p.38)

18 19
2‘-MOE RNA DMTO Base
REAGENT CARTRIDGE O
NHBz
H3C
NHBz O
H3C
O

N N
N N NH NH

N N N N N N
O NHiBu O

O-MOE
Technical Specifications Phosphoramidite Adenosine 5-Methyl-Cytidine Guanosine 5-Methyl-Uridine

Phosphoramidite for building 2’-MOE RNA oligonucleotides

Volume 150nt Chemistry

In 2’-O-methoxyethyl (2’-MOE) RNA phosphoramidites, the proton on the
Nucleotides 2’-O-methoxyethyl ribonucleotides 2’-ribose position is substituted with a methoxyethyl group. This substitution
stabilizes an A-shaped helix during hybridization due to its electronegativity,
Bases Quantity 75 x A, C, G, mU making a 2’-MOE RNA/RNA duplex significantly more stable.

5’-Modification No Advantages
In comparison to natural RNA, RNA strands synthesized using 2′-MOE phos-
Usage Fully modified RNA synthesis phoramidites exhibit improved duplex stability and significantly increased
nuclease resistance.
Shelf Life 1 year
Potential applications
Combining the properties of nuclease resistance and its ability to bind more
Shelf Life after Activation 1 week efficiently to the target sequence, the 2’-MOE modification is a highly advanta-
geous approach for the development of RNA-affecting therapeutics.
Short-Term Storage 2 months at 2-8°C

Long-Term Storage -20°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one-XT

What’s inside?
Price
The cartridge contains all the reagents
required for chemical RNA synthesis. This EUR 900,00 / USD 1,100.00
includes the bases A, C, G and U, which are RNA Bases

Fully Modified RNA Oligos

positioned in the center of the cartridge with
colored caps. The presence of an excess A C G mU
amount of bases ensures the availability of Chip Combination
all four phosphoramidites until the final
synthesis.

Standard Chip (p.28)

20 21
2‘-OMe RNA DMTO Base
REAGENT CARTRIDGE O
NHBz NHAc O O

N N
N N NH NH

N N N N N N
O Ndmf O

O-Me
Technical Specifications Phosphoramidite Adenosine Cytidine Guanosine Uridine

Phosphoramidite for building 2’-OMe RNA oligonucleotides

Volume 150nt Chemistry

In 2’-O-methyl (2’-OMe) RNA phosphoramidites the proton on the 2’-ribose
Nucleotides 2’-O-methyl ribonucleotides position is substituted with a methyl group. This substitution stabilizes an
A-shaped helix during hybridization due to its electronegativity, making a
Bases Quantity 75 x A, C, G, U 2’-OMe RNA/RNA duplex significantly more stable.

5’-Modification No Advantages
RNA strands synthesized using 2’-OMe phosphoramidites exhibit improved
Usage Fully modified RNA synthesis duplex stability and significantly increased nuclease resistance.

Potential applications
Shelf Life 1 year
Combining the properties of nuclease resistance and their ability to bind more
efficiently to the target sequence, the 2’-OMe modification is a highly advanta-
Shelf Life after Activation 1 week geous approach for the development of RNA-affecting therapeutics.

Short-Term Storage 2 months at 2-8°C

Long-Term Storage -20°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one-XT

What’s inside?
The cartridge contains all the reagents required
Price
for chemical RNA synthesis. This includes the EUR 900,00 / USD 1,100.00
bases A, C, G and U, which are positioned in the RNA Bases
center of the cartridge with colored caps. The
presence of an excess amount of bases ensures A C G U
the availability of all four phosphoramidites until Chip Combination
the final synthesis.

Standard Chip (p.28)

22 23
2‘-F RNA DMTO Base
REAGENT CARTRIDGE O
NHBz NHAc O O

N N
N N NH NH

N N N N N N
O NHiBu O

F
Technical Specifications Phosphoramidite Adenosine Cytidine Guanosine Uridine

Phosphoramidite for building 2’-fluoro RNA oligonucleotides

Volume 150nt Chemistry

2’-fluoro (2’-F) RNA phosphoramidites contain a fluorine at the 2’-ribose posi-
Nucleotides 2’-fluoro ribonucleotides tion instead of a 2’-hydroxyl group. Because of their similar sizes, the substitu-
tion with fluorine does not affect the strand conformation, but it stabilizes an
Bases Quantity 75 x A, C, G, U A-shaped helix during hybridization due to its electronegativity, making a 2’-F
RNA/RNA duplex significantly more stable.
5’-Modification No
Advantages
Usage Fully modified RNA synthesis RNA strands synthesized using 2′-F phosphoramidites exhibit improved duplex
stability and significantly increased nuclease resistance.
Shelf Life 1 year
Potential applications
Combining the properties of nuclease resistance and their ability to bind more
Shelf Life after Activation 1 week efficiently to the target sequence, the 2’-F modification is a highly advanta-
geous approach for the development of RNA-affecting therapeutics.
Short-Term Storage 2 months at 2-8°C

Long-Term Storage -20°C

Scope of Delivery 1 reagent cartridge

Required Kilobaser one-XT

What’s inside?
The cartridge contains all the reagents required
Price
for chemical RNA synthesis. This includes the EUR 900,00 / USD 1,100.00
bases A, C, G and U, which are positioned in the RNA Bases
center of the cartridge with colored caps. The
presence of an excess amount of bases ensures A C G T
the availability of all four phosphoramidites until Chip Combination
the final synthesis.

Standard Chip (p.28)

24 25
CUSTOMIZED
REAGENT CARTRIDGE Strand elongation
3’- CABBTBTBGBCATCGACCTATC -5’

5’- ATTCAGTCGAAGTSSASASCSG -3’

Strand elongation
Technical Specifications
Example: A different base pair was added into the sequence for hybridization

Volume 100 - 150nt Possible modifications

There are different ways to customize the cartridge according to your needs:
Nucleotides As requested
• Replacing the bases
Bases Quantity 75 x A, C, G, T/U • Adding an additional reagent
- Adding a 5th base for internal modification
5’-Modification As requested - Adding a 5’-modification
• Replacing the oxidizing agent to obtain thiolated DNA or RNA oligonucleotides
Usage Custom modified DNA/RNA
This cartridge is compatible with various chip types for further modifications.
Custom cartridges are freshly prepared for each order and sent out with the corre-
Shelf Life Depends
sponding chips. For inquiries about using your own reagents with Kilobaser, please
contact us directly for further details.
Shelf Life after Activation Depends

Short-Term Storage Depends

Long-Term Storage Depends

Scope of Delivery As requested

Required Kilobaser one-XT

Additional base or 5’-modification

What’s inside?
Price
The cartridge contains all the reagents required
for chemical DNA and RNA synthesis, respectively. on request
It will be filled with customized reagents, including Bases
the bases in the center of the cartridge as well as a
further modification typically positioned in the top

Customized Cartridge
left corner. Chip Combination

Depends

26 27
STANDARD RFU x 10⁵
12
MICROFLUIDIC CHIP
10

6
Technical Specifications
4

Properties Single-use; microcolumn incl. 2

Chip Type Microfluidic 0

Cycle
-2
End Product Unlabeled DNA oligo 0 5 10 15 20 25 30 35 40

Material Polymer Kilobaser primers Service provider primers

Amplification curves of SYBR green qPCR performed with Kilobaser primers (purple) and service
Shelf Life 1 year provider primers (blue) show nearly identical progress and signal intensity.

Short-Term Storage Dry; room temperature What’s in it for you?

A typical application of the standard chip is the synthesis of PCR primers (=unmodified
Long-Term Storage Dry; room temperature DNA strands). The resulting primers can be applied directly after synthesis without
any additional purification. However, if the desired application requires DNA or RNA of
Scope of Delivery 1 microfluidic chip increased purity, we highly recommend the Kilobaser OliPure HIC Purification Kit (p.40).

Required Any cartridge; Kilobaser one or What is the quality of the synthesized oligonucleotides?
Kilobaser one-XT In a direct qPCR comparison, as shown in the plot above, typically no differences can
be detected to primers ordered from a service provider.

Custom sample oligos of a desired sequence can be ordered from our webshop to
verify the quality of the oligonucleotides we provide.

Heart of the synthesis: the chip

The microfluidic chip provides the reaction chamber as well as channels to trans-
port the reagents. To regulate the reagent flow, gas pressure is applied to the chip
membrane. The membrane covers the entire chip and must not be removed.
Price
EUR 15,00 / USD 18.00
A fresh chip each time
The chip is built for single use to ensure the yield and to avoid cross-contamination
with former syntheses.

Microfluidic Chips
What can you do with it?
The standard chip is used to yield oligonucleotides without any terminal modifica-
tions and can be combined with any cartridge, as it is suitable for both DNA and
RNA synthesis.

28 29
6-FAM LABEL
MICROFLUIDIC CHIP

Technical Specifications

Properties Single-use; microcolumn incl.

Chip Type Microfluidic

End Product 5’-6-FAM labeled DNA oligo Single-labeled PCR primer for fragment analysis

Material Polymer What’s in it for you?

A typical application of the 6-FAM label chip is the synthesis of fluorescent PCR primers.
Shelf Life 1 year These primers can be used for PCR, resulting in PCR amplicons that are labeled with
fluorophore. This labeling allows the detection of the PCR amplicons during capillary
Short-Term Storage Dry; room temperature electrophoresis, as seen in the figure above.

Long-Term Storage Dry; room temperature The 6-FAM labeled primers can be applied directly after synthesis without any additional
purification. However, if the desired application requires oligonucleotides of increased
purity, use the Kilobaser OliPure HIC Purification Kit (p.40).
Scope of Delivery 1 microfluidic chip
What is the quality of the synthesized oligonucleotides?
Required 6-FAM cartridge; Kilobaser one-XT
Custom sample oligos of a desired sequence can be ordered from our webshop to
verify the quality of the oligonucleotides we provide.

Heart of the synthesis: the chip

The microfluidic chip provides the reaction chamber as well as channels to transport
the reagents. To regulate the reagent flow, gas pressure is applied to the chip membrane.
Price
The membrane covers the entire chip and must not be removed. EUR 75,00 / USD 90.00

A fresh chip each time

The chip is built for single use to ensure the yield and to avoid cross-contamination
with former syntheses.

What can you do with it?

The 6-FAM label chip is used in combination with the 6-FAM cartridge (p.12) to yield
oligonucleotides with the 6-FAM at the 5’-end.

30 31
BHQ-1 QUENCHER RFU x 10⁴
250
MICROFLUIDIC CHIP
200

150

Technical Specifications 100

50
Properties Single-use; microcolumn incl.
0
Chip Type Microfluidic
Cycle
-50
End Product 5’-6-FAM / 3’-BHQ-1 labeled DNA oligo 0 5 10 15 20 25 30 35 40

Kilobaser oligos Service provider oligos

Material Polymer
Amplification curves of qPCR performed with Kilobaser primer/probe sets (purple) and service
Shelf Life 6 months provider primer/probe sets (blue) show nearly identical progress and signal intensity.

Short-Term Storage Dry; 2-8°C; dark What’s in it for you?

The BHQ-1 quencher chip enables synthesis of dual-labeled oligos, such as TaqMan®
Long-Term Storage Dry; 2-8°C; dark probes for qPCR. As non-coupled 6-FAM can interfere with qPCR signal levels, we
recommend to purify the probes with the Kilobaser OliPure HIC Purification Kit (p.40).
Scope of Delivery 1 microfluidic chip; OliPure HIC Purification Kit
What is the quality of the synthesized oligonucleotides?
Required 6-FAM cartridge; Kilobaser one-XT In a typical qPCR reaction, as shown in the figure above, no differences to primers and
probes ordered from a service provider can be detected.

Custom sample oligos of a desired sequence can be ordered from our webshop to
verify the quality of the oligonucleotides we provide.

Heart of the synthesis: the chip

The microfluidic chip provides the reaction chamber as well as channels to transport
the reagents. To regulate the reagent flow, gas pressure is applied to the chip membrane.
The membrane covers the entire chip and must not be removed.
Price
EUR 95,00 / USD 115.00
A fresh chip each time
The chip is built for single use to ensure the yield and to avoid cross-contamination
with former syntheses.

What can you do with it?

The BHQ-1 quencher chip is used in combination with the 6-FAM cartridge (p.12) to
synthesize dual-labeled oligonucleotides with the quencher at the 3’-end and the
6-FAM at the 5’-end.
Visit our application note here:
http://oligo.link/application-bhq1

32 33
AMINO MODIFIER C6
MICROFLUIDIC CHIP H2N

Isothiocyanate
group
Carboxyl Aldehyde Sulfonic Epoxy
group group group group S
Technical Specifications O O O H2C O C
OH
OH H
C C S CH N

O
Properties Single-use; microcolumn incl.

Chip Type Microfluidic

End Product 5’-amino C6 labeled DNA oligo Covalent immobilization of amino-modified DNA via different functionalized groups

Material Polymer What’s in it for you?

The amino-group can react with a wide range of functional groups such as carboxyl,
Shelf Life 1 year aldehyde, sulfonic, epoxy and isothiocyanate. Such groups can be prepared on surfaces
allowing covalent immobilization of amino-modified oligonucleotides as shown in the
Short-Term Storage Dry; room temperature figure above.

Long-Term Storage Dry; room temperature 5‘-amino C6 labeled DNA oligonucleotides can be applied directly after synthesis. None-
theless, additional purification with the Kilobaser OliPure HIC Purification Kit (p.40) may
improve the results of the experiments.
Scope of Delivery 1 microfluidic chip
What is the quality of the synthesized oligonucleotides?
Required Amino Modifier C6 cartridge;
Custom sample oligos of a desired sequence can be ordered from our webshop to
Kilobaser one-XT
verify the quality of the oligonucleotides we provide.

Heart of the synthesis: the chip

The microfluidic chip provides the reaction chamber as well as channels to transport
the reagents. To regulate the reagent flow, gas pressure is applied to the chip membrane.
Price
The membrane covers the entire chip and must not be removed. EUR 35,00 / USD 42.00

Use a fresh chip each time!

The chip is built for single use to ensure the yield and to avoid cross-contamination
with former syntheses.

What can you do with it?

The Amino Modifier C6 chip is used in combination with the Amino Modifier C6 cartridge
(p.14) to synthesize amino-modified DNA oligonucleotides.

34 35
BIOTIN LABEL
MICROFLUIDIC CHIP

Technical Specifications

Properties Single-use; microcolumn incl.

Chip Type Microfluidic

End Product 5’-biotin labeled DNA oligo Biotin-labeled DNA on magnetic beads bind target DNA strands

Material Polymer What’s in it for you?

Biotin binds highly specific to streptavidin or avidin, which can be prepared on the
Shelf Life 1 year surface of e.g. multi-well plates or magnetic beads, allowing the immobilization of bi-
otinylated oligonucleotides. The immobilized oligonucleotides can still hybridize with
Short-Term Storage Dry; room temperature complementary strands, as shown in the figure above. This hybridization can be used
for DNA screening and purification.
Long-Term Storage Dry; room temperature
5’-biotin labeled DNA oligonucleotides can be applied directly after synthesis. How-
ever, an additional purification with Kilobaser OliPure HIC Purification Kit (p.40) might
Scope of Delivery 1 microfluidic chip
improve the results of the experiments.
Required Biotin cartridge; Kilobaser one-XT
What is the quality of the synthesized oligonucleotides?
Custom sample oligos of a desired sequence can be ordered from our webshop to
verify the quality of the oligonucleotides we provide.

Heart of the synthesis: the chip

The microfluidic chip provides the reaction chamber as well as channels to transport
the reagents. To regulate the reagent flow, gas pressure is applied to the chip membrane.
Price
The membrane covers the entire chip and must not be removed. EUR 60,00 / USD 72.00

Use a fresh chip each time!

The chip is built for single use to ensure the yield and to avoid cross-contamination
with former syntheses.

What can you do with it?

The Biotin label chip is used in combination with the Biotin cartridge (p.16) to synthesize
5’-biotinylated DNA oligonucleotides.

36 37
PHOSPHORYLATION
MICROFLUIDIC CHIP Phosphate

Technical Specifications

Properties Single-use; microcolumn incl.

Chip Type Microfluidic

End Product 5’-phosphorylated DNA oligo Phosphorylated oligonucleotide guides endonuclease

Material Polymer What’s in it for you?

In ligation reactions, the ligase enzyme can only connect two strands efficiently if
Shelf Life 1 year the phosphate group is present. 5’-phosphorylated oligos are directly suitable for
ligation. Another application are prokaryotic argonaute endonucleases. Phospho-
Short-Term Storage Dry; room temperature rylated oligos are applied as guides to cut DNA strands at a desired site, as shown
in the figure above. Oligos made with this chip type are ideally suited for cutting and
Long-Term Storage Dry; room temperature ligation in vector editing.

The resulting oligos can be applied directly after synthesis without any additional
Scope of Delivery 1 microfluidic chip
purification. If the desired application does require DNA or RNA of increased purity,
Kilobaser OliPure HIC Purification Kit (p.40) allows for quick and convenient purification.
Required Phosphorylation cartridge;
Kilobaser one-XT
What is the quality of the synthesized oligonucleotides?
Custom sample oligos of a desired sequence can be ordered from our website to
verify the quality of the oligonucleotides we provide.

Heart of the synthesis: the chip

The microfluidic chip provides the reaction chamber as well as channels to transport
the reagents. To regulate the reagent flow, gas pressure is applied to the chip membrane. Price
The membrane covers the entire chip and must not be removed. EUR 25,00 / USD 30.00

Use a fresh chip each time!

The chip is built for single use to ensure the yield and to avoid cross-contamination
with former syntheses.

What can you do with it?

The Phosphorylation chip is used in combination with the Phosphorylation cartridge
(p.18) to synthesize 5’-phosphorylated DNA oligonucleotides.

38 39
OliPure HIC Absorption [OD]
2
PURIFICATION KIT
1,6

1,2
Technical Specifications
0,8

Properties HIC column based oligonucleotide purification

0,4
Throughput 20μL – 120μL sample volume
0
Min Oligo Length 12nt 400 440 480 520 560 600 640
Wavelength [nm]
Max Oligo Concentration 250ng/μL
Unpurified Kilobaser probe Purified Kilobaser probe Service provider probe
Recovery Rate 75% (±5%)

Shelf Life 1 year Absorbance spectra of three double-labeled (6-FAM+BHQ-1) DNA probes at the same concentration,
but purified in different ways: probe synthesized with Kilobaser one-XT unpurified (black) and purified
with Kilobaser OliPure HIC Purification Kit (purple) and a HPLC purified probe from a service provider
Storage 2-8°C
(blue). The unpurified probe shows the highest signal at 495nm, indicating unbound dye from oligo-
nucleotide synthesis. Purification of the probe using the OliPure HIC Purification Kit reduces unbound
Application Purification of oligonucleotides after synthesis 6-FAM to the same low levels seen in HPLC-purified probes.
or post-synthesis modifications

Scope of Delivery 10 x Spin-down Tubes with HIC column;

1 x Instruction

Price
Required Microcentrifuge
EUR 90,00 / USD 110.00
(fixed-angle or swing-out bucket rotors)

What do you get?

The OliPure HIC Purification Kit is a hydrophobic interaction chromatography (HIC) purification
column that utilizes the properties of phenyl agarose to bind impurities (e.g.: salt, unbound
dyes). Oligonucleotides pass directly through the column in a single spin-down.

What can you do with it?

The OliPure HIC Purification Kit is optimized for the purification of oligonucleotides synthesized
with a Kilobaser device. It works for both DNA and RNA oligonucleotides and thus with all our
cartridges. If you plan to use the purification kit for other samples, contact us for support.

Supply
40 41
 Get in Touch

[email protected]
+43 660 3939 772
www.kilobaser.com
Reininghausstrasse 13a,
8020 Graz, Austria, Europe

UNLOCKING THE POWER

OF DNA & RNA SYNTHESIS FOR ALL