Experiment 5 UV VIS Post Lab
Experiment 5 UV VIS Post Lab
Experiment 5 UV VIS Post Lab
Aa : 0 Ab : 0.808
2. From the spectrum of the measuring bottle (9), find the wavelength at which
the absorption is maximum as the detection wavelength (λ1), and use pH
versus absorption degree [A] Drawing. Such as schematic diagram (1).
Fig 1. pH vs [A] graph
pH vs [A]
0.9
0.8
0.7
0.6
[A] at λ1
0.5
0.4
0.3
0.2
0.1
0
1.65 4.49 6.18 6.5 7.14 7.6 7.9 9.01 11.33
pH
3. Plotting the pH value as horizontal axis and log([In-] / [HIn]) on the vertical
axis to find the linear relationship.
1 y = 0.3275x - 0.3487
R² = 0.9891
0.5
0
7.14 7.6 7.9 9.01
-0.5
pH
Week 2
1. For acidic environment, pH is constant, and the dye concentration changes.
Calculation:
[C]
Mw: 624.38 g/mol
𝐶 = 4 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 6.4 × 10-6
𝐶 = 6 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 9.6 × 10-6
𝐶 = 8 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 1.28 × 10-5
𝐶 = 10 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 1.6 × 10-5
To calculate [X]:
Hln ↔ H+ + ln-
1-x x x
(1-x) x x
To Calculate [ln-]* :
[ln-]* = x[C]
(1) [ln-]* = x[C] = 2.428 x 6.4 x 10-6 = 1.554 x 10-5
(2) [ln-]* = x[C] = 2.052 x 9.6 x 10-6 = 1.970 x 10-5
(3) [ln-]* = x[C] = 2.124 x 1.28 x 10-5 = 2.718 x 10-5
(4) [ln-]* = x[C] = 1.8 x 1.6 x 10-5 = 2.88 x 10-5
To Calculate [Hln]* :
[Hln]* : (1-x)[C]
(1) [Hln]* : (1-x)[C] = (1-2.428) x 6.4 x 10-6 = -9.14 x 10-6
(2) [Hln]* : (1-x)[C] = (1-2.052) x 9.6 x 10-6 = -1.010 x 10-5
(3) [Hln]* : (1-x)[C] = (1- 2.124) x 1.28 x 10-5 = -1.439 x 10-5
(4) [Hln]* : (1-x)[C] = (1-1.8) x 1.6 x 10-5 = -1.28 x 10-5
Fig 3. Results for absorption at maximum wavelength λ2
0.06
0.04
0.02
0
0 6.4 9.6 12.8 16
Concentration * 10^-6 (mol/L)
Calculation:
[C]
Mw: 624.38 g/mol
𝐶 = 4 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 6.4 × 10-6
𝐶 = 6 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 9.6 × 10-6
𝐶 = 8 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 1.28 × 10-5
𝐶 = 10 𝑚𝑔/𝐿 × 1 𝑔/1000 𝑚𝑔 × 1 𝑚𝑜𝑙 /624.38 𝑔 = 1.6 × 10-5
To calculate [X]:
Hln ↔ H+ + ln-
1-x x x
(1-x) x x
To Calculate [ln-]* :
[ln-]* = x[C]
(5) [ln-]* = x[C] = 0.0127 x 6.4 x 10-6 = 8.128 x 10-8
(6) [ln-]* = x[C] = 0.0241 x 9.6 x 10-6 = 2.313 x 10-7
(7) [ln-]* = x[C] = 0.0321 x 1.28 x 10-5 = 4.109 x 10-7
(8) [ln-]* = x[C] = 0.0488 x 1.6 x 10-5 = 7.808 x 10-7
To Calculate [Hln]* :
[Hln]* : (1-x)[C]
(5) [Hln]* : (1-x)[C] = (1-0.0127) x 6.4 x 10-6 = 6.319 x 10-6
(6) [Hln]* : (1-x)[C] = (1-0.0241) x 9.6 x 10-6 = 9.368 x 10-6
(7) [Hln]* : (1-x)[C] = (1- 0.0321) x 1.28 x 10-5 = 1.239 x 10-5
(8) [Hln]* : (1-x)[C] = (1-0.0488) x 1.6 x 10-5 = 1.522 x 10-5
Fig 5. Results for absorption at maximum wavelength λ1
0.02
0.015
0.01
0.005
0
0 6.4 9.6 12.8 16
Concentration * 10^-6 (mol/L)
From my experience I think this experiment is very cool because I get to learn
about this new machine, the UV-Vis spectrometer and the pH meter. Furthermore,
the things that I learnt during my instrumental analysis classes help me a lot to do
this experiment. During the experiment, we also still dealing with some problems
and error when we use the instruments. I think those error is caused by our own
negligence during mixing the mixture. Also, I think this is one of the longest
experiments that I have done this semester.
The worst things that happen when we do this experiment is we have done some
error during the experiment, that makes our results in the reports very strange too.
I think it is because we didn’t change the range to 615 nm, instead we still use 500
nm.
2. In the experiment, when the dye concentration is constant and the pH value is
changed, there is an obvious intersection point near the wavelength of 500 nm
in the spectrum diagram. What is the physical significance of this point?
• A reagent called bromothymol blue is used to determine the weak acids and
bases in a pH range of 6.4 to 8.0. In an acidic environment, it turns yellow,
while in a basic environment, it turns blue. The figure below shows that at a
wavelength of 500 nm, there is a clear intersection point. This indicates that
the absorbance remains constant at all pH values, indicating that the molar
absorptivity of [In-] and [Hln] is equal.
IV. References
[1] College, S. M. (n.d.), LibreTexts Chemistry, Detection and Absorption of
Ultraviolet Light (Experiment):
https://chem.libretexts.org/Ancillary_Materials/Laboratory_Experiments/Wet_Lab
_Experiments/General_Chemistry_Labs/Online_Chemistry_Lab_Manual/Chem_9
_Experiments/04%3A_Detection_and_Absorption_of_Ultraviolet_Light_(Experi
ment