DKSGACA Manual No.

01/2021

Protected Cultivation and Secondary Agriculture

A Laboratory Manual

Prepared by
Dr. Mahesh Prasad Tripathi

Dr. Khem Singh Gill Akal College of Agriculture

Eternal University, Baru Sahib,
Sirmour-173101, Himachal Pradesh, India
2021
 DKSGACA Manual No. 01/2021

Protected Cultivation and Secondary Agriculture

A Laboratory Manual

Prepared by
Dr. Mahesh Prasad Tripathi

Approved vide item no. 74/19/2021 in the Academic Council Meeting held on 15/06/2021

Dr. Khem Singh Gill Akal College of Agriculture

Eternal University, Baru Sahib,
Sirmour-173101, Himachal Pradesh, India
2021
 Preface

The present manual Protected Cultivation and Secondary Agriculture is for B.Sc. (Hons.) Agriculture
students of Indian Universities. Depending on the local climate and the bioclimatic requirements of the
species to be cultivated, once the proper site has been selected, it will be necessary to choose the
cladding material, the type of structure and the architectonic shape of the greenhouse. If the predictable
climate generated by the greenhouse is not appropriate complementary facilities and equipment for
climate control will have to be considered. Greenhouse design is very much influenced, in practice, by
the local climate and the latitude of the site and in many cases is limited by the availability of materials
for the construction. Protected Cultivation activities are also ideally situated for generating employment
for the unskilled workers of rural areas.

It is hoped that the present manual will be useful for the students, researchers, extension workers
as well as practicing engineers. Suggestions and comments are welcome for further improvement of this
manual.

Eternal University Mahesh P. Tripathi

2021
 INDEX

Ex.
No. Name of Exercise Page No.

1. Study of different type of Greenhouses based on shape.

1-3

2. Determine the rate of air exchange in an Active summer winter

cooling system. 4

3. Determination of drying rate of Agricultural products inside

Green house. 5-9

4. Study of Greenhouse equipment’s.

10-12

5. Determination of Moisture content of various grains by oven

drying & infrared moisture methods. 13-14

6. Determination of Engineering properties (shape and size, bulk

density and porosity of biomaterials). 15-18

7. Determination of Moisture content of various grains by

moisture meter. 19-20

8. Field visit to seed processing plant

21

9. Visit to various Post Harvest Laboratories. 22

 EXERCISE – 1

STUDY OF DIFFERENT TYPE OF GREENHOUSES BASED ON SHAPE

Green House:
A greenhouse is a framed or inflated design covered with a transparent or translucent material in
which crops could be developed under the conditions of at least a partially controlled
environment and which is sufficiently enough to allow people to work inside it to carry out
cultural operations.
Greenhouse type based on shape
Greenhouses can be classified based on their shape or style. For the purpose of classification, the
uniqueness of the cross-section of the greenhouses can be considered as a factor. As the
longitudinal area tend to be approximately the same for all types, the longitudinal area of the
greenhouse cannot be used for classification. The cross-sections portray the width and height of
the structure and the length is vertical to the plane of cross-section. Also, the cross-section
provides information on the overall shape of the structural members, such as truss or hoop, which
will be repeated on every day. The usually followed types of greenhouse dependent on shape are
lean-to, even span, uneven span, ridge and furrow, saw tooth and Quonset.
1. Lean-to type greenhouse
A lean-to design is used when a greenhouse is located against the side of an existing structure. It
is build against a building, using the existing structure for at least one of its sides. It is generally
connected to a house yet might be joined to different buildings. The roof of the building is
extended with appropriate greenhouse covering material and the area is properly enclosed. It is
typically facing south side. The lean-to type greenhouse is limited to single or double-row plant
benches with a total width of 7 to 12 feet. It can be as long as the building it is attached to. It
should experience the best direction for sufficient sun exposure. The advantage of the lean-to -
type greenhouse is that, it usually is close to accessible electricity, water, and heat. It is the most
affordable structure. This design makes the best use of daylight and minimizes the requirement of
ceiling supports. It has the following disadvantages: limited space, limited light, limited
ventilation and temperature control. The height of the supporting wall limits the potential size of
the design. Temperature control is more difficult because the wall that the greenhouse is built on,

1
may collect the sun's heat while the translucent cover of the greenhouse may lose heat rapidly. It
is a half greenhouse, split along the peak of the roof.
2. Even span type greenhouse
The even-span is the standard type and full-size structure, the two roof slopes are of equivalent
pitch and width. This design is utilized for the greenhouse of small size, and it is constructed on
tabulated ground. It is connected to a house at one gable end. It can provide accommodation for 2
or 3 rows of plant benches. The cost of an even-span greenhouse is more than the cost of a lean-
to- type, however it has more noteworthy adaptability in design and provides for more plants.
Because of its size and greater amount of exposed glass area, the even-span will cost more to
heat. The design has a preferable shape over a lean-to type for air circulation to maintain uniform
temperatures during the winter heating season. A separate heating system is essential unless the
structure is near to a heated building. It will house 2 side benches, 2 walks, and a wide center
bench. A few single and multiple span types are accessible for use in various regions of India.
For single span type the span in general, varies from 5 to 9 m, whereas the length is around 24 m
with 2.5 to 4.3 m height.
3. Uneven span type greenhouse
This type of greenhouse is constructed on hilly terrain. The ceilings are of different width; make
the structure adjustable to the side slopes of hill. This type of greenhouses is seldom used now-a-
days as it is not adaptable for automation.
4. Ridge and furrow type greenhouse
Designs of this type use two or more A-frame greenhouses conjoined to each other along the
length of the eave. The eave serves as furrow or gutter to carry rain and melted snow away. The
side wall is eliminated between the greenhouses, which fallout in a structure with a single large
interior, Consolidation of interior space reduces labour, lowers the cost of automation, improves
personal management and decreases fuel consumption as there is less exposed wall area through
which heat escapes. The snow loads must be taken into the frame specifications of these
greenhouses since the snow cannot slide off the roofs as in case of individual free standing
greenhouses, but melts away. In spite of snow loads, ridge and furrow greenhouses are
effectively used in northern countries of Europe and in Canada and are well suited to the Indian
conditions.

2
5. Saw tooth type Greenhouse

These are likewise indistinguishable from the ridge and furrow type greenhouses accept that,
there is prerequisite for natural ventilation in this type. Specific natural ventilation flow path
creates in a saw- tooth type greenhouse.
6. Quonset greenhouses
This is a greenhouse, where the pipe arches or trusses are supported by pipe purling running
along the length of the greenhouse. In general, the covering material utilized for this type of
greenhouses is polyethylene. Such greenhouses are normally less expensive than gutter
connected greenhouses and are useful when a small isolated cultural area is required. These
houses are linked either in free, standing style or arranged in an interlocking ridge and furrow. In
the interlocking type, truss members overlap sufficiently to allow a bed of plants to grow
between the overlapping portions of adjacent houses. A single large cultural space thus exists for
a set of houses in this type, an arrangement that is better adapted to the automation and
movement of labour.

3
 EXERCISE - 2
DETERMINE THE RATE OF AIR EXCHANGE IN AN ACTIVE SUMMER WINTER
COOLING SYSTEM
Air flow calculation
The airflow leaving an inflated tube through a smooth circular opening can be considered
according to the equation: Airflow (cfm) = Constant * Coefficient of Discharge * Area in Square Feet
* the square root of the static pressure within the tube in inches of water.
Q = 4005X CX AX P1/2
Q = Airflow in cfm for circular openings (0.60)
C = Coefficient of discharge for circular openings (0.60)
A = Area of opening in square feet (for a circle: πd2/4)
P = Static pressure in inches of water
4005: Constant for air at standard temperature and pressure

Air Exchange Capacity of a Ventilation System

One way to calculate the air volume being stimulated by a ventilation system is to determine air
speed and cross sectional area during which air is moving.
Air speed (feet per minute, fpm) x Area (sq. ft.) = ventilation rate (cubic feet per minute, cfm).
The air speed moving through the fan and/or inlets should be calculated To determine cross-
sectional area, the fan airflow opening should be measured, which is usually the fan diameter, or
sum the total inlet areas.

4
 EXERCISE - 3
DETERMINATION OF DRYING RATE OF AGRICULTURAL PRODUCTS INSIDE
GREEN HOUSE

Drying process can be divided in to three periods:

(i) Constant drying rate period and
(ii) (ii) First falling drying rate period and (iii) (iii) Second falling
rate period.

(i) Constant drying rate period

In a constant drying rate period, a material or mass of material contain so much water
that liquid surface exists will dry in a manner comparable to an open faced body of water.
Diffusion of moisture from within the droplet maintains saturated surface conditions and as
long as these last, evaporation takes place at constant rate. When a solid is dried under
constant drying conditions, the moisture content ×t typically falls the graph is linear at first,
then curves and eventually levels off. Constant rate drying period will proceed until free
moisture appears from the surface, the moisture removal rate will then become progressively
less. The moisture content at which the drying rate ceases to be constant is known as the
critical moisture content. During the constant rate period, the moisture from interior migrates
to the surface by various means and is vaporized. As the moisture content is lowered, the rate
of migration to the surface is lowered. If drying occurs at too high temperatures, the surface
forms the layer of closely packed shrunken cells which are sealed together. This presents a
barrier to moisture migration and tends to keep the moisture sealed within. This condition is
known as „case hardening‟.
The constant rate period is characterized by a rate of drying independent of moisture content.
During this period, the solid is so wet that a continuous film of water exists over the entire
drying surface, and this water acts as if solids were not there. The temperature of the wetted
surface attained the wet bulb temperature.

5
 Web bulb Temperature (WBT)
WBT is the steady state temp shown by the thermometer whose bulb is covered with a wet
wick and from which water is evaporating into a high velocity air stream. The quantity of
water evaporated is not high enough to alter the temperature and humidity of the air stream.
The air blown at high velocity (minimum recommended is 300 m/min). It causes evaporation
of water from the wick. Evaporation requires latent heat. This heat comes from surface of
glass bulb of thermometer. So the temperature of the glass bulb decreases. The the heat
comes from the temperature difference between Tw and Ta (large). It is the case of
simultaneous heat and mass transfer. This heat is latent heat for phase change of water to
water vapor.

q = amount of latent heat transfer

q = Mw Nw λw A ----------------- (i)

Mw = mol. Mass of water kg/kg mol

Nw = molar flu× of water vapour, kg mol m-2 s-1

λw = Latest heat of vaporization kJ/kg

A = surface area of the bumb m2

6
Nw = ky (yw-y)

ky = mass transfer coefficient kg mol m-2 s-1

yw = mole fraction of water vapour in the stagnant air layer adjacent to the wet cloth

y = mole fraction of water vapour in the air stream, some distance away from the wet cloth

(yw-y) is the driving force.

Where,

w = kg of moisture Ø = time h = heat transfer coefficient between

air and moisture kcal/ kg hr °C ta = dry bulb temperature of air, °C
ts = surface temperature, °C A= area, m2
ΔHv = heat of vapourisation at ts ,Kcal/ kg ka
= mass transfer coefficient (kg/ hr m)
Hs = humidity of saturated air at the surface temperature
Ha = humidity of air

The constant rate period ends when the migration rate of water from the interior of the surface
becomes less than the rate of evaporation from the surface. The period subsequent to the critical
point is called „the falling rate period‟. Beyond this point, the surface temperature rises, and the
drying rate falls off rapidly. The falling rate period takes a far longer time than the constant rate
period, even though the moisture removal may be much less. The drying rate approaches zero at
some equilibrium moisture content.

Drying in falling rate period involves two processes:

a) movement of moisture within the material to the surface
b) removal of the moisture from the surface

7
 The method used to estimate drying rates and drying times in the falling rate period depends on
whether the solid is porous or non-porous. In a non-porous material, once there is no superficial
moisture, further drying can occur only at a rate governed by diffusion of internal moisture to the
surface. In a porous material other mechanism appears, and drying may even takes place inside the
solid instead of at the surface.

(ii) First falling drying rate period

Point B, the moisture content at the end of the constant rate period, is the „critical moisture content‟.
At this point the surface of the solid is no longer saturated, and the rate of drying decreases with the
decrease in moisture content. At point C, the surface moisture film has evaporated fully, and with the
further decrease in moisture content, the drying rate is controlled by the rate of moisture movement
through the solid.
(iii) Second falling drying rate period

Period C to D represents conditions when the drying rate is largely independent of conditions outside
the solid. The moisture transfer may be by any combination of liquid diffusion, capillary movement,
and vapor diffusion.
11.2 Estimation of Drying Time

In order to determine the time required to achieve the desired reduction in product moisture content, the
rate of moisture removal or drying rate must be predicted. The rate of drying depends on properties of
drying air (the dry bulb temperature, RH, and velocity of air and the surface heat transfer coefficient), the
properties of food (moisture content, surface to volume ratio and the surface temperature) and rate of
moisture loss. The size of the pieces has an important effect on the drying rate in both the constant and
falling rate periods. In the constant rate period, smaller pieces have a larger surface area available for
evaporation where as in falling rate period smaller pieces have a shorter distance for moisture to travel
through the food. Other factors which influence the rate of drying include:

8
1. The fat content of the food (higher fat contents generally results in slower drying, as water is
trapped within the food).

2. The method of preparation of food (cut pieces lose moisture more quickly than losses through
skin.
3. The amount of food placed in a dryer in relation to its size (in a given dryer faster drying is
achieved with smaller quantities of food).

For constant rate drying period the following general expression would apply:
Rc = dw / dt = wo – wc / tc--------------- (1)

Where,
wc = Critical moisture content (kg water / kg dry solid ) and

tc = Time for constant rate drying

During falling rate drying, the following analysis would apply.

- dw/dt = Rc / wc (w) or
wc / Rc ò - dw /w = ò dt
Where the limits of integration are between critical moisture content wc or end of constant rate
drying, tc and some desired final moisture content, w.

On integration:

t –tc = wc/Rc ln(wc/w) or time for falling rate becomes

tf = wc/Rc × ln (wc/w) ------------- (3) and

The total drying times becomes

t = (wo - wc) /Rc + Wc/Rc × ln (wc/w) ---------- (4)

The above equation indicates that the time for complete drying from some initial moisture content
wo to a desirable final moisture content w depends on knowledge of critical moisture content wc,
the time for constant rate drying tc, and the rate for constant drying Rc.

9
 EXERCISE - 4
STUDY OF GREENHOUSE EQUIPMENTS

List of greenhouse equipments:

1. Screens: It helps control the amount of light, humidity and temperature inside the facility,
which turns to an improvement of the crop conditions and a reduction of the energy costs.

The screen has a flexible and easily-folding structure that once folded takes up minimum space
and allows entering the maximum amount of light.

It may be automatically run by means of a solar radiation sensor.

Hot air heating: The hot air generators are especially recommended in those cases where there
is not an important requirement for continuous heating and as an occasional defense against
freezing temperatures. The purpose of this system is to increase the productivity of the crops and
their maturity in cold weather, using medium level technology.

The distribution of hot air is carried out using fans and hoses.

Water heating: This is a Centralized heat generation system using natural gas, diesel, biomass,
geothermal heat. Water circulates through metal or PVC-Polyethylene pipes as a heat
transporting agent, depending on the temperature of the hot water source, the temperature
increase requirements and the crop.

We offer a wide range of solutions in this area, from basic systems, to the most sophisticated
systems with Open Buffer heat storage systems and use of CO2 coming from combustion gases.

Extractor fan: The extractor fans allow forcing the ventilation inside greenhouses when the
natural ventilation using roof and/or perimeter vents, does not allow reaching the desired rate of
air renewal, which is an innate need for producing crops as well as livestock farms.

They are often used in combination with evaporative cooling panels or water misting systems for
the purpose of obtaining a certain level of cooling.

10
Air circulation fan: The air circulation fans or recirculation fans help obtain a suitable air
movement contributing to maintain a homogeneous interior climate, avoiding hot air
accumulation at the upper section of the greenhouse, reducing substantially the degree of water
condensation and favouring the crops‟ transpiration and CO2 absorption.

They may be used as support for the extractor fans or as humidifier systems or for applying
treatments.

Cooling: This water evaporation cooling system is comprised of extractor fans and cooling
panels installed on opposite walls of the greenhouse to create a negative pressure area inside the
greenhouse. This forces the outside air flowing through the dampened panels becoming charged
with water molecules and cooled down and thus decreases the temperature inside the greenhouse.
Fertigation: Fertigation consists of applying simultaneously water and fertilizers through the
irrigation system, supplying the nutrients required by the crops to the soil or substrate.
Fertigation is especially useful in the case of drip irrigation. By means of automatic high
technology fertigation equipment the water and the nutrients are perfectly placed in the
absorption area of the roots, improving the rate of growth and quality of the crops.

This system allows carrying out a more rational use of the water and fertilizers, respecting the
environment and minimizing the environmental impact.

Growing benches: The growing benches may be fixed, mobile or transportable.

Fix benches have a standard working height of 80cm.

The transportable benches include multi-direction wheels with brakes. Mobile benches allow
moving the bench platform sideways on the structure, which facilitates creating aisles to access
the benches, thus optimizing the surface used for the growing.

Depending on the purpose for which the bench is used, any of the above-mentioned models can
be selected with metallic grid bottom or an ebb and flow bottom.

Fog system: Consists of incorporating a large number of micro-particles of water to the ambient
air, which remain suspended in the air inside the greenhouse long enough to evaporate without
wetting the crops. The water is added in the form of fog using special nozzles distributed
uniformly all over the surface of the greenhouse.

11
The Fog system is very useful for humidifying and cooling down the greenhouse in a controlled
manner and carrying out disinfection treatments using soluble plant protection products.

Inflatable roof: The double inflated film system consists of creating an air chamber between two
layers of plastic. The air chamber is kept inflated using small fans that inject air into the chamber
via PVC and flexible pipes.

This air chamber reduces the heat transmission coefficient towards the outside, achieving a
considerable energy savings and temperature control.

This system may be used on roofs as well as along the perimeter.

Climate control: offers a wide range of climate controllers for the automated management of all
the systems that are installed in our greenhouses.

The controllers are guided by the information collected by the different sensors installed, in order
to maintain suitable levels of solar radiation, temperature, relative humidity and CO2
concentration for the crop; thus achieving the best evolution of the crops regarding their
performance, early maturity and quality.

12
 EXERCISE - 5
DETERMINATION OF MOISTURE CONTENT OF VARIOUS GRAINS BY OVEN
DRYING & INFRARED MOISTURE METHODS

Moisture: Moisture content was estimated using A.O.A.C. (2006) method. Accurately weighed 5
g sample was taken in previously dried and weighed petri dish. The petri dish along with sample
was placed in the oven maintained at 85±1°C for 3 to 4 h, by repeating the process of drying,
cooling in the desiccator and weighing at 30 min intervals, until the difference between
consecutive weights was less than 1mg. Then it was transferred to desiccators and cooled and
weighed. The percent moisture content was calculated as:

W2−W
Moisture content (%) = –––––––––––– × 100
W1−W

Where; W = Weight of empty petri dish

W1 = Weight of petri dish with sample before drying
W2 = Weight of petri dish with sample after drying to constant weight Infra-Red
moisture meter:

The near infrared reflectance, NIR or IR, technique is a widely used technology for online
moisture testing. Its popularity is due in large part to the ease with which it can be applied.

A light source (typically quartz halogen bulb) is collimated and filtered into specific
wavelengths. The filters, mounted in a rotating wheel, chop the light into a series of pulses of
specific wavelength. The filtered beam is directed onto the surface of the product to be measured.
A portion of the light is reflected back to a detector (usually lead sulfide). Specific wavelengths
of light are absorbed by water. If the filters are chosen such that one wavelength will be absorbed
by water (sample beam) and one wavelength will be unaffected by water (reference beam), then

13
the amplitude ratio of the two reflected wavelengths will be proportionate to the amount of water
in the product. The ratio technique eliminates effects of product distance and source aging.
• Ease of application. Typically mounted 6 to 10 inches above product. Moderate product
height variations have little influence on measurement.
• Small spot measurement area in conjunction with scanning frame provides product profile.
• Specific wavelengths may be chosen to measure variables other than moisture.

14
 EXERCISE - 6
DETERMINATION OF ENGINEERING PROPERTIES (SHAPE AND SIZE, BULK
DENSITY AND POROSITY OF BIOMATERIAL)

1. Grain size and shape

Grain size and shape (length-width ratio) is a very stable varietal property that can be used to
measure the varietal purity of a sample. Comparing the length-width ratio of the sample with a
published ratio for the variety will give an indication of varietal purity of the grain sample. A
significant deviation means that the sample is impure – that is, it is either a different variety or a
mixture of varieties.

Obtain a random sample from the seed batch.

Collect 20 grains at random from this sample of seed.

Use a Vernier caliper or photographic enlarger to measure the dimensions of each grain.

15
 2. 1000 grain weight

Each variety has a published weight for 1000 grains. If the 1000-grain weight calculated from
the sample departs from this, it may be an indication that the sample contains a mixture of
varieties.

Select a random sample from the seed batch Count

1,000 whole grains from the sample.

Weigh the 1,000 grains.

3. Bulk Density
Bulk density was determined by liquid displacement method. Fifty ml distilled water was
taken in the measuring cylinder and volume was noted. Fifty g seeds were weighed accurately
and transferred to the cylinder. Increase in volume of water was recorded to calculate bulk
density and expressed as g/cc.
4. Hydration Capacity
Seeds weighing 10 g were counted and transfer to a measuring cylinder. To this 30 ml water
was added and cylinder was enclosed with aluminum foil and left overnight at room temperature.
Next day, seeds were drained, superfluous water was removed with filter paper and swollen
seeds were reweighed.
Increase in weight x100
Hydration capacity (%) =
Weight (g) of seeds

Increase in weight
Hydration capacity (per seed) =
Number of seeds

16
 5. Hydration index
Hydration index was calculated using the following formula:
Hydration capacity per seed
Hydration index =
Weight (g) of one seed
6. Swelling capacity
Seeds weighing 10 gm were counted and their volume was noted and soaked overnight.
The volume of soaked seeds were noted in graduated cylinder.

Increase in volume x100

Swelling capacity (%) =
Weight (g) of seeds

Volume after soaking-Volume before soaking

Swelling capacity (per seed) =
Number of seeds

7. Swelling index
Swelling index was calculated using the formula:
Swelling capacity per seed
Swelling index =
Seed volume (ml)

8. Porosity: (inter granular space):

Introduction and Definition: Total porosity is defined as the fraction of the bulk rock volume V
that is not occupied by solid matter. If the volume of solids is denoted by Vs, and the pore
volume as Vp = V - Vs, we can write the porosity as:
ϕ= V – VS/V = VP/V
Pore Volume/Total Bulk Volume
The porosity can be expressed either as a fraction or as a percentage. Two out of the three terms
are required to calculate porosity. It should be noted that the porosity does not give any
information concerning pore sizes, their distribution, and their degree of connectivity.
17
Thus, rocks of the same porosity can have widely different physical properties. An example of
this might be a carbonate rock and a sandstone. Each could have a porosity of 0.2, but carbonate
pores are often much unconnected resulting in its permeability being much lower than that of the
sandstone.
Laboratory Determinations: There are many methods for measuring porosity, a few of which
will be discussed below. Several standard techniques are used. In themselves these are basic
physical measurements of weight, length, and pressures. The precision with which these can be
made on plugs is affected by the nature (particularly surface texture) of the plugs.

1 Direct Measurement Here the two volumes V and Vs are determined directly and used in Eq.
(1). This method measures the total porosity, but is rarely used on rocks because Vs can only be
measured if the rock is totally disaggregated, and cannot, therefore, be used in any further petro
physical studies. This measurement is the closest laboratory measurement to density log derived
porosities.
2 Imbibition Method the rock sample is immersed in a wetting fluid until it is fully saturated.
The sample is weighed before and after the imbibition, and if the density of the fluid r is known,
then the difference in weight is r Vp , and the pore volume Vp can be calculated. The bulk
volume V is measured using either vernier calipers and assuming that the sample is perfectly
cylindrical, or by Archimedes Method (discussed later), or by fluid displacement using the
saturated sample. Vp and V can then be used to calculate the connected porosity. This is an
accurate method that leaves the sample fully saturated and ready for further petrophysical tests.
The time required for saturation depends upon the rock permeability.

18
 EXERCISE - 7
DETERMINATION OF MOISTURE CONTENT OF VARIOUS GRAINS BY MOISTURE
METER

Moisture meters: All usually used methods are stemmed on electrical property of beans. An
electrical current unit, resistance or capacitance, is considered and then transformed into
moisture content.

Resistance: The meter measures the electrical resistance of beans when a current is apply
between two electrodes. Beans are positioned in a constant and known volume.
Capacitance: the meter measures an electrical current between two plates of a condenser
which constitute the walls of a recipient A precise weight of sample is essential. In both
techniques, temperature rectifying is necessary for accurate measurements. Most of moisture
meters are equipped with temperature correction software.
Limits of the method: calibration charts must be reputable for each grain type. This means that
a meter must be calibrated individually for robusta beans and arabica beans, but also for
cherries and parchment to attain accurate measurements. Accurate measurements are obtained
within a range given by the manufacturer. Over this range, readings are pointless.

LOSS ON DRYING MOISTURE METER METHOD (LOD):

The original primary moisture measurement method was Loss on Drying (LOD). In an LOD
test, the sample is weighed, dried, and weighed again. The variation in the two weights (Loss
on Drying) is then collated with either the original weight (Wet-base test) or final weight (Dry-
base test) and the moisture content calculated. Tests can be physically conducted (weigh, oven
dry, weigh) or automated (integrated weight and heating unit) with systems called Moisture
Determination Balances. Depending on the balance and heating mechanism, a wide array of
precision and accuracy is available. Today there are even micro moisture analyzers, using
microbalances that can provide moisture measurement to the PPM level, consistent with the
limits of KF testing.
19
Drawbacks of LOD test method are that it is destructive, meaning the sample is altered by the
heating. In addition, it may be time-consuming with some tests taking 30 minutes or more to
complete. Further, the test makes the assumption that all weight loss is due to water. In cases
where substantial other volatiles (organics) are also available, this may not be the truth.
Therefore, the results will overstate the moisture content and the test temperatures must be kept
low to avoid excessive loss of these “non-water” based components. Lowering the temperature
increases the test time. Finally, the samples are dried with people involved. Where there is heat
involved, safety and labor costs may become a concern.

20
 EXERCISE - 8
FIELD VISIT TO SEED PROCESSING PLANT VISIT

21
 EXERCISE - 9
VISIT TO VARIOUS POST HARVEST LABORATORIES

22