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Army Public School & College (Boys) Saddar Karachi

APSIS Session 2023-24
Subject: Biology P4

Name: _ M. Marks: 40
Class/Sec: OIII A/D Time allowed: 1 hr
1 Some students investigated the activity of catalase.

Catalase is an enzyme found in plant and animal tissues. It catalyses the breakdown of hydrogen
peroxide into water and oxygen. The activity of this enzyme can be measured by collecting the oxygen
produced.

2H2O2  2H2O  O2
hydrogen peroxide water oxygen

The students used the apparatus shown in Fig.1.1 to compare the activity of catalase using
piecesof sweet potato of different sizes.

delivery tube
bung

foam water bubbles

of oxygen
sweet potato cube

hydrogen peroxide solution

Fig. 1.1

Three cubes of 10 mm  10 mm  10 mm of sweet potato were used.

Cube 1 was placed into the test-tube and 5 cm3 of hydrogen peroxide was added.The bung was quickly
replaced into the top of the test-tube. The students carefully counted the number of bubbles of oxygen
delivered into the water in oneminute and then measured the final height of foam in the test-tube.
For cube 1, they counted 12 bubbles in one minute. The height of foam is shown in Fig. 1.2.They then
discarded the contents of the test-tube and rinsed it with clean water.
Cube 2 was cut into two pieces of approximately the same size.Both of these pieces were placed into the
test-tube and 5 cm3 of hydrogen peroxide was added asbefore.
For cube 2, they counted 23 bubbles in one minute. The height of foam is shown in Fig. 1.2.They then
discarded the contents of the test-tube and rinsed it with clean water.
Cube 3 was cut into eight small pieces which were all placed into the test-tube and 5 cm3 ofhydrogen
peroxide was added as before.
For cube 3, they counted 38 bubbles in one minute. The height of foam is shown in Fig. 1.2.
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height of foam

foam

cube 1 cube 2 cube 3

drawn to scale

Fig. 1.2

(a) In the space below, prepare a table for the results from this investigation.
Use a ruler to measure the height of foam in Fig. 1.2 for each cube.
Record the height of foam for each cube in your table.

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B (i) State two variables that the students kept constant in this investigation.

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(ii) Suggest how you could improve the method that they followed.

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C What conclusion can be drawn from these results.

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(i) The students were given a hypothesis that said

“the activity of catalase increases with increasing temperature”.

Describe a similar investigation to the one carried out in (a) to test this hypothesis.

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 (iii) Outline one safety precaution that you would need to take when performing thisexperiment.

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[Total: 19]

2 Fig. 2.1 shows the lower surface of a leaf from a dicotyledonous plant, on a 1 cm2 grid.

(i) Make a large, labelled drawing of the leaf in Fig. 2.1

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(ii)Calculate the surface area of the leaf in Fig. 2.1.

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(iii) Describe the method you used to determine the surface area of the leaf.

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(a) Fig. 2.2 shows the upper and lower surfaces of the same leaf of another dicotyledonous plant.

upper surface lower surface

Fig. 2.2

Describe two ways, visible in Fig. 2.2, in which the upper surface of the leaf is different fromthe lower
surface.

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(b) A student investigated the effect of different wind speeds on the rate of transpiration of some
leaves.
The student took five leaves from a tree and weighed each of them on a balance.
Each of the leaves was then hung from a different piece of wire.
Fans were used to blow air at different speeds over each leaf.
After 12 hours, the student weighed each leaf again. The results are shown in Table 2.1.

Table 2.1

wind speed mass of leaf atthe mass of leaf atthe loss in mass
/ m per s start / g end / g /g
0 5.7 3.8
1 5.3 3.3
3 5.9 3.7
6 5.1 2.6
8 5.3 2.6

(i) Calculate the loss in mass for each leaf. Complete Table 2.1. [2]

(ii) Construct a graph to show the loss in mass against wind speed.

(iii) Use your graph to determine the loss in mass at a wind speed of 10 m per s. Show on the
graph how you obtained your answer.

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(iv) A student criticised the results by saying that the loss in mass does not allow for a fair
comparison between leaves.

Suggest a more appropriate calculation and explain why it gives a fairer comparison.

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[Total: 21]
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