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    T1W04 Mutations WS

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    tigernath06
    A gene mutation is a permanent change in the DNA sequence that codes for a protein. There are several types of mutations including substitutions, insertions, deletions, and more. According to the document, a substitution mutation changed a base but did not alter the amino acid sequence. A deletion mutation removed a base, changing all subsequent codons and amino acids. An insertion mutation added a base, altering all following codons. The effect was to introduce a stop codon, truncating the protein.

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    T1W04 Mutations WS

    Uploaded by

    tigernath06
    21 views6 pages
    A gene mutation is a permanent change in the DNA sequence that codes for a protein. There are several types of mutations including substitutions, insertions, deletions, and more. According to the document, a substitution mutation changed a base but did not alter the amino acid sequence. A deletion mutation removed a base, changing all subsequent codons and amino acids. An insertion mutation added a base, altering all following codons. The effect was to introduce a stop codon, truncating the protein.

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    T1W04 Mutations WS (2)

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    A gene mutation is a permanent change in the DNA sequence that codes for a protein. There are several types of mutations including substitutions, insertions, deletions, and more. According to the document, a substitution mutation changed a base but did not alter the amino acid sequence. A deletion mutation removed a base, changing all subsequent codons and amino acids. An insertion mutation added a base, altering all following codons. The effect was to introduce a stop codon, truncating the protein.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    21 views6 pages

    T1W04 Mutations WS

    Uploaded by

    tigernath06
    A gene mutation is a permanent change in the DNA sequence that codes for a protein. There are several types of mutations including substitutions, insertions, deletions, and more. According to the document, a substitution mutation changed a base but did not alter the amino acid sequence. A deletion mutation removed a base, changing all subsequent codons and amino acids. An insertion mutation added a base, altering all following codons. The effect was to introduce a stop codon, truncating the protein.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 6

    EXPLAIN Mutations Name:_______________

    Term Definition
    Mutation

    Gene mutation
    Mutagen
    Chromosome
    mutation
    Somatic
    mutation
    Germ line
    mutation

    Read the information on Gene Mutations and answer the questions.

    Type of Gene Description of Mutation Effect/s on amino acid sequence


    (Point) Mutation
    Substitution
    Silent
    substitution
    Insertion

    Deletion

    mRNA Codon and corresponding amino acid table

    Normal Gene
    Non- mutated DNA AAA ATG TTT CTC CAA GAT

    mRNA UUU UAC AAA GAG GUU CUA

    Amino acid sequence coded for

    Gene Mutation Problem 1: Substitution


    In the following DNA sequence replace the G of the second codon with an A to create a new mutant DNA.
    Determine the new mRNA sequence and the amino acid sequence.

    Mutant DNA AAA ATA TTT CTC CAA GAT

    mRNA UUU UAU AAA GAG GUU CUA

    Amino acid sequence coded for

    a) Describe the effect of the substitution mutation on the amino acid sequence of the protein
    Gene Mutation Problem 2: Deletion
    In the following DNA sequence, a mutation of the same gene from the last example, a deletion mutation has
    occurred. Refer to the “normal gene” on the previous page.

    Mutant DNA AAA ATA TTC TCC AAG ATA

    mRNA UUU UAU AAG AGG UUC UAU

    Amino acid sequence coded for

    b) Annotate (draw an arrow) showing the location of the deletion mutation.

    c) Describe the effect on the amino acid sequence.

    Gene Mutation Problem 3: Determine the type and effect


    In the following DNA sequence, a mutation of the same gene from the last example has occurred. Refer to the
    “normal gene” on the previous page.

    Mutant DNA AAA ATG TTT ACT CCA AGA

    mRNA UUU UAC AAA UGA GGU UCU

    Amino acid sequence coded for

    d) Annotate (draw an arrow) showing the location of the mutation.

    e) Name this type of mutation.

    f) Describe the effect of this mutation.

    g) Is this mutation likely to change the protein coded for in this gene? Explain why/why not.
    EXPLAIN Mutations Name:_______________

    Term Definition
    Mutation A permanent change in the sequence of bases in the DNA

    Gene mutation A permanent change in the sequence of bases in the DNA that code
    for a protein.
    Mutagen Anything that causes a mutation, e.g.: radiation, chemicals, viruses
    Chromosome A mutation that affects the whole chromosome or a large part of it.
    mutation
    Somatic A mutation that occurs in a body cell.
    mutation
    Germ line A mutation that occurs in the sex cells
    mutation

    Read the information on Gene Mutations and answer the questions.


    Type of Gene Description of Mutation Effect/s on amino acid sequence in protein
    (Point) Mutation being coded for
    Substitution One base replaces another in May change one amino acid in a protein.
    the sequence

    Silent One base replaces another in the None


    sequence with no change in the
    substitution sequence of amino acids.

    Insertion An extra base is added to the All of the codons are changed from that point on, many
    sequence. or all of the following amino acids will change

    Deletion A base is removed from the All of the codons are changed from that point on, many
    sequence or all of the following amino acids will change

    mRNA Codon and corresponding amino acid table

    Normal Gene
    Non- mutated DNA AAA ATG TTT CTC CAA GAT

    mRNA UUU UAC AAA GAG GUU CUA

    Amino acid sequence coded for Phe Tyr Asn Asp Val Leu

    Gene Mutation Problem 1: Substitution


    In the following DNA sequence replace the G of the second codon with an A to create a new mutant DNA.
    Determine the new mRNA sequence and the amino acid sequence.

    Mutant DNA AAA ATA TTT CTC CAA GAT

    mRNA UUU UAU AAA GAG GUU CUA

    Amino acid sequence coded for Phe Tyr Asn Asp Val Leu

    a) Describe the effect of the substitution mutation on the amino acid sequence of the protein
    None, it is a silent mutation
    Gene Mutation Problem 2: Deletion
    In the following DNA sequence, a mutation of the same gene from the last example, a deletion mutation has
    occurred. Refer to the “normal gene” on the previous page.

    Mutant DNA AAA ATA TTC TCC AAG ATA

    mRNA UUU UAU AAG AGG UUC UAU

    Amino acid sequence coded for Phe tyr lys arg phe Tyr

    b) Annotate (draw an arrow) showing the location of the deletion mutation.

    c) Describe the effect on the amino acid sequence.


    That ammino acid, and everyone after, is changed

    Gene Mutation Problem 3: Determine the type and effect


    In the following DNA sequence, a mutation of the same gene from the last example has occurred. Refer to the
    “normal gene” on the previous page.

    Mutant DNA AAA ATG TTT ACT CCA AGA

    mRNA UUU UAC AAA UGA GGU UCU

    Amino acid sequence coded for phe tyr lys stop gly ser

    d) Annotate (draw an arrow) showing the location of the mutation.

    e) Name this type of mutation.


    insertion

    f) Describe the effect of this mutation.

    The bene is stopped from being read at that point. The protein is therefore cut short, as it is a shorter ammino acid
    sequence.

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